Antitumor effects in vitro and in vivo and mechanisms of protection against melanoma B16F10-Nex2 cells by fastuosain, a cysteine proteinase from Bromelia fastuosa
Autor(a) principal: | |
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Data de Publicação: | 2007 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1593/neo.07427 http://hdl.handle.net/11449/34176 |
Resumo: | In the present work, the antitumor effect of fastuosain, a cysteine proteinase from Bromelia fastuosa, was investigated. In the intravenous model of lung colonization in C57Bl/6 mice, fastuosain and bromelain injected intraperitoneally were protective, and very few nodules of B16F10-Nex2 melanoma cells were detected. Tumor cells treated with fastuosain showed reduced expression of CD44 and decreased invasion through Matrigel, lost their cytoplasmic extensions and substrate adherence, and became round and detached, forming strongly bound cell clusters in suspension. Peritoneal cells recruited and activated by fastuosain treatment ( mainly monocytic cells and lymphocytes) migrated to the lung, where pulmonary melanoma metastases grew. Adoptive transference of peritoneal cells recruited by fastuosain had no protective effect against lung metastases in recipient mice. Treatment of green fluorescent protein - chimeric animals with fastuosain did not change the number of cells that migrated to the lung, compared to PBS-injected control mice, but the number of positive major histocompatibility complex class II cells increased with fastuosain treatment. Murine antibodies against fastuosain, bromelain, and cathepsins B and L cross-reacted in ELISA and recognized surface and cytoplasmic components expressed on B16F10-Nex2 cells. Anti-fastuosain antibodies were cytotoxic/lytic to B16F10-Nex2 cells. Antitumor effects of fastuosain involve mainly the direct effect of the enzyme and elicitation of protective antibodies. |
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Antitumor effects in vitro and in vivo and mechanisms of protection against melanoma B16F10-Nex2 cells by fastuosain, a cysteine proteinase from Bromelia fastuosafastuosainbromelainB16F10-Nex2 tumor cellscathepsins B/Lprotective antibodiesIn the present work, the antitumor effect of fastuosain, a cysteine proteinase from Bromelia fastuosa, was investigated. In the intravenous model of lung colonization in C57Bl/6 mice, fastuosain and bromelain injected intraperitoneally were protective, and very few nodules of B16F10-Nex2 melanoma cells were detected. Tumor cells treated with fastuosain showed reduced expression of CD44 and decreased invasion through Matrigel, lost their cytoplasmic extensions and substrate adherence, and became round and detached, forming strongly bound cell clusters in suspension. Peritoneal cells recruited and activated by fastuosain treatment ( mainly monocytic cells and lymphocytes) migrated to the lung, where pulmonary melanoma metastases grew. Adoptive transference of peritoneal cells recruited by fastuosain had no protective effect against lung metastases in recipient mice. Treatment of green fluorescent protein - chimeric animals with fastuosain did not change the number of cells that migrated to the lung, compared to PBS-injected control mice, but the number of positive major histocompatibility complex class II cells increased with fastuosain treatment. Murine antibodies against fastuosain, bromelain, and cathepsins B and L cross-reacted in ELISA and recognized surface and cytoplasmic components expressed on B16F10-Nex2 cells. Anti-fastuosain antibodies were cytotoxic/lytic to B16F10-Nex2 cells. Antitumor effects of fastuosain involve mainly the direct effect of the enzyme and elicitation of protective antibodies.Univ Fed São Paulo, UNONEX, Unidade Oncol Expt, Dept Microbiol Immunol & Parasitol, BR-04023062 São Paulo, BrazilUniv Fed São Paulo, Discipline Parasitol, Dept Microbiol Immunol & Parasitol, BR-04023062 São Paulo, BrazilUniv Fed São Paulo, IBILCE, Dept Quim & Ciências Ambientais, BR-04023062 São Paulo, BrazilFiocruz MS, Ctr Pesquisas Goncalo Moniz, Salvador, BA, BrazilUniv Fed São Paulo, IBILCE, Dept Quim & Ciências Ambientais, BR-04023062 São Paulo, BrazilNeoplasia PressUniversidade Federal de São Paulo (UNIFESP)Universidade Estadual Paulista (Unesp)Fiocruz MSGuimaraes-Ferreira, Carla A.Rodrigues, Elaine G.Mortaray, Renato A.Cabralz, HamiltonSerrano, Fabiana A.Ribeiro-dos-Santos, RicardoTravassos, Luiz R.2014-05-20T15:23:23Z2014-05-20T15:23:23Z2007-09-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article723-733http://dx.doi.org/10.1593/neo.07427Neoplasia. Ann Arbor: Neoplasia Press, v. 9, n. 9, p. 723-733, 2007.1522-8002http://hdl.handle.net/11449/3417610.1593/neo.07427WOS:000249552100004Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengNeoplasia2,133info:eu-repo/semantics/openAccess2021-10-22T17:11:50Zoai:repositorio.unesp.br:11449/34176Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-22T17:11:50Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Antitumor effects in vitro and in vivo and mechanisms of protection against melanoma B16F10-Nex2 cells by fastuosain, a cysteine proteinase from Bromelia fastuosa |
title |
Antitumor effects in vitro and in vivo and mechanisms of protection against melanoma B16F10-Nex2 cells by fastuosain, a cysteine proteinase from Bromelia fastuosa |
spellingShingle |
Antitumor effects in vitro and in vivo and mechanisms of protection against melanoma B16F10-Nex2 cells by fastuosain, a cysteine proteinase from Bromelia fastuosa Guimaraes-Ferreira, Carla A. fastuosain bromelain B16F10-Nex2 tumor cells cathepsins B/L protective antibodies |
title_short |
Antitumor effects in vitro and in vivo and mechanisms of protection against melanoma B16F10-Nex2 cells by fastuosain, a cysteine proteinase from Bromelia fastuosa |
title_full |
Antitumor effects in vitro and in vivo and mechanisms of protection against melanoma B16F10-Nex2 cells by fastuosain, a cysteine proteinase from Bromelia fastuosa |
title_fullStr |
Antitumor effects in vitro and in vivo and mechanisms of protection against melanoma B16F10-Nex2 cells by fastuosain, a cysteine proteinase from Bromelia fastuosa |
title_full_unstemmed |
Antitumor effects in vitro and in vivo and mechanisms of protection against melanoma B16F10-Nex2 cells by fastuosain, a cysteine proteinase from Bromelia fastuosa |
title_sort |
Antitumor effects in vitro and in vivo and mechanisms of protection against melanoma B16F10-Nex2 cells by fastuosain, a cysteine proteinase from Bromelia fastuosa |
author |
Guimaraes-Ferreira, Carla A. |
author_facet |
Guimaraes-Ferreira, Carla A. Rodrigues, Elaine G. Mortaray, Renato A. Cabralz, Hamilton Serrano, Fabiana A. Ribeiro-dos-Santos, Ricardo Travassos, Luiz R. |
author_role |
author |
author2 |
Rodrigues, Elaine G. Mortaray, Renato A. Cabralz, Hamilton Serrano, Fabiana A. Ribeiro-dos-Santos, Ricardo Travassos, Luiz R. |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Federal de São Paulo (UNIFESP) Universidade Estadual Paulista (Unesp) Fiocruz MS |
dc.contributor.author.fl_str_mv |
Guimaraes-Ferreira, Carla A. Rodrigues, Elaine G. Mortaray, Renato A. Cabralz, Hamilton Serrano, Fabiana A. Ribeiro-dos-Santos, Ricardo Travassos, Luiz R. |
dc.subject.por.fl_str_mv |
fastuosain bromelain B16F10-Nex2 tumor cells cathepsins B/L protective antibodies |
topic |
fastuosain bromelain B16F10-Nex2 tumor cells cathepsins B/L protective antibodies |
description |
In the present work, the antitumor effect of fastuosain, a cysteine proteinase from Bromelia fastuosa, was investigated. In the intravenous model of lung colonization in C57Bl/6 mice, fastuosain and bromelain injected intraperitoneally were protective, and very few nodules of B16F10-Nex2 melanoma cells were detected. Tumor cells treated with fastuosain showed reduced expression of CD44 and decreased invasion through Matrigel, lost their cytoplasmic extensions and substrate adherence, and became round and detached, forming strongly bound cell clusters in suspension. Peritoneal cells recruited and activated by fastuosain treatment ( mainly monocytic cells and lymphocytes) migrated to the lung, where pulmonary melanoma metastases grew. Adoptive transference of peritoneal cells recruited by fastuosain had no protective effect against lung metastases in recipient mice. Treatment of green fluorescent protein - chimeric animals with fastuosain did not change the number of cells that migrated to the lung, compared to PBS-injected control mice, but the number of positive major histocompatibility complex class II cells increased with fastuosain treatment. Murine antibodies against fastuosain, bromelain, and cathepsins B and L cross-reacted in ELISA and recognized surface and cytoplasmic components expressed on B16F10-Nex2 cells. Anti-fastuosain antibodies were cytotoxic/lytic to B16F10-Nex2 cells. Antitumor effects of fastuosain involve mainly the direct effect of the enzyme and elicitation of protective antibodies. |
publishDate |
2007 |
dc.date.none.fl_str_mv |
2007-09-01 2014-05-20T15:23:23Z 2014-05-20T15:23:23Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1593/neo.07427 Neoplasia. Ann Arbor: Neoplasia Press, v. 9, n. 9, p. 723-733, 2007. 1522-8002 http://hdl.handle.net/11449/34176 10.1593/neo.07427 WOS:000249552100004 |
url |
http://dx.doi.org/10.1593/neo.07427 http://hdl.handle.net/11449/34176 |
identifier_str_mv |
Neoplasia. Ann Arbor: Neoplasia Press, v. 9, n. 9, p. 723-733, 2007. 1522-8002 10.1593/neo.07427 WOS:000249552100004 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Neoplasia 2,133 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
723-733 |
dc.publisher.none.fl_str_mv |
Neoplasia Press |
publisher.none.fl_str_mv |
Neoplasia Press |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1803650283574657024 |