Role of cytokines, NO, and H2O2 on the immunopathology of Leptospirosis in genetically selected mice

Detalhes bibliográficos
Autor(a) principal: Marinho, M. [UNESP]
Data de Publicação: 2005
Outros Autores: Langoni, H. [UNESP], Oliveira, S. L. [UNESP], Lima, V. M. F. [UNESP], Peiró, J. R. [UNESP], Perri, S. H. V. [UNESP], Carreira, R. [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1590/S1678-91992005000200009
http://hdl.handle.net/11449/211855
Resumo: Immune response to leptospirosis is mainly humorally mediated, and involves opsonization of leptospires for phagocytosis by macrophages and neutrophils. However, some aspects are still unknown. For a more detailed analysis of the cellular immune response to leptospirosis infection, trials were carried out in order to determine the hydrogen peroxide and nitric oxide (H2O2 and NO) production stimulated or not by Interferon-gamma. The participation of some specific cytokines, such as Tumor Necrosis Factor-alpha (TNF-alpha); Interferon-gamma (IFN-gamma); Interleukin-6 (IL-6); and Interleukin-4 (IL-4), in the immunopathology of this infection was also investigated. For this purpose, we analyzed the supernatant from peritoneal macrophage cell culture and the splenic cells of mice genetically selected as High (H) and Low (L) antibody producers, and inbred Balb/c mice infected with Leptospira interrogans serovar icterohaemorrhagiae. The IL-6 production varied from release peaks to inhibition in H, L, and Balb/c mice. Similar behavior was observed for IL-4, produced only by H and Balb/c mice. The three strains presented constant and elevated production of TNF-alpha until day 14, suggesting its effective participation in the initial phase of the infection. Meanwhile, all the three strains presented a constant and irregular IFN-gamma production, with release peaks between the 7th and 14th days in L mice. The H and Balb/c mice strains presented a higher tendency to Th2 response pattern, whereas L mice tended towards Th1 response.
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spelling Role of cytokines, NO, and H2O2 on the immunopathology of Leptospirosis in genetically selected miceleptospirosiscytokinescellular immunityImmune response to leptospirosis is mainly humorally mediated, and involves opsonization of leptospires for phagocytosis by macrophages and neutrophils. However, some aspects are still unknown. For a more detailed analysis of the cellular immune response to leptospirosis infection, trials were carried out in order to determine the hydrogen peroxide and nitric oxide (H2O2 and NO) production stimulated or not by Interferon-gamma. The participation of some specific cytokines, such as Tumor Necrosis Factor-alpha (TNF-alpha); Interferon-gamma (IFN-gamma); Interleukin-6 (IL-6); and Interleukin-4 (IL-4), in the immunopathology of this infection was also investigated. For this purpose, we analyzed the supernatant from peritoneal macrophage cell culture and the splenic cells of mice genetically selected as High (H) and Low (L) antibody producers, and inbred Balb/c mice infected with Leptospira interrogans serovar icterohaemorrhagiae. The IL-6 production varied from release peaks to inhibition in H, L, and Balb/c mice. Similar behavior was observed for IL-4, produced only by H and Balb/c mice. The three strains presented constant and elevated production of TNF-alpha until day 14, suggesting its effective participation in the initial phase of the infection. Meanwhile, all the three strains presented a constant and irregular IFN-gamma production, with release peaks between the 7th and 14th days in L mice. The H and Balb/c mice strains presented a higher tendency to Th2 response pattern, whereas L mice tended towards Th1 response.Universidade Estadual Paulista, Department of Animal Health and ProductionUniversidade Estadual Paulista, Araçatuba School of DentistryUniversidade Estadual Paulista, Institute of BiosciencesUniversidade Estadual Paulista, Department of Animal Health and ProductionUniversidade Estadual Paulista, Araçatuba School of DentistryUniversidade Estadual Paulista, Institute of BiosciencesCentro de Estudos de Venenos e Animais PeçonhentosUniversidade Estadual Paulista (Unesp)Marinho, M. [UNESP]Langoni, H. [UNESP]Oliveira, S. L. [UNESP]Lima, V. M. F. [UNESP]Peiró, J. R. [UNESP]Perri, S. H. V. [UNESP]Carreira, R. [UNESP]2021-07-14T10:30:42Z2021-07-14T10:30:42Z2005-06info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article198-212application/pdfhttp://dx.doi.org/10.1590/S1678-91992005000200009Journal of Venomous Animals and Toxins including Tropical Diseases. Botucatu, SP, Brazil: Centro de Estudos de Venenos e Animais Peçonhentos, v. 11, n. 2, p. 198-212, 2005.1678-9199http://hdl.handle.net/11449/21185510.1590/S1678-91992005000200009S1678-91992005000200009S1678-91992005000200009.pdfSciELOreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Venomous Animals and Toxins including Tropical Diseasesinfo:eu-repo/semantics/openAccess2023-12-31T06:16:18Zoai:repositorio.unesp.br:11449/211855Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T21:45:11.511274Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Role of cytokines, NO, and H2O2 on the immunopathology of Leptospirosis in genetically selected mice
title Role of cytokines, NO, and H2O2 on the immunopathology of Leptospirosis in genetically selected mice
spellingShingle Role of cytokines, NO, and H2O2 on the immunopathology of Leptospirosis in genetically selected mice
Marinho, M. [UNESP]
leptospirosis
cytokines
cellular immunity
title_short Role of cytokines, NO, and H2O2 on the immunopathology of Leptospirosis in genetically selected mice
title_full Role of cytokines, NO, and H2O2 on the immunopathology of Leptospirosis in genetically selected mice
title_fullStr Role of cytokines, NO, and H2O2 on the immunopathology of Leptospirosis in genetically selected mice
title_full_unstemmed Role of cytokines, NO, and H2O2 on the immunopathology of Leptospirosis in genetically selected mice
title_sort Role of cytokines, NO, and H2O2 on the immunopathology of Leptospirosis in genetically selected mice
author Marinho, M. [UNESP]
author_facet Marinho, M. [UNESP]
Langoni, H. [UNESP]
Oliveira, S. L. [UNESP]
Lima, V. M. F. [UNESP]
Peiró, J. R. [UNESP]
Perri, S. H. V. [UNESP]
Carreira, R. [UNESP]
author_role author
author2 Langoni, H. [UNESP]
Oliveira, S. L. [UNESP]
Lima, V. M. F. [UNESP]
Peiró, J. R. [UNESP]
Perri, S. H. V. [UNESP]
Carreira, R. [UNESP]
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Marinho, M. [UNESP]
Langoni, H. [UNESP]
Oliveira, S. L. [UNESP]
Lima, V. M. F. [UNESP]
Peiró, J. R. [UNESP]
Perri, S. H. V. [UNESP]
Carreira, R. [UNESP]
dc.subject.por.fl_str_mv leptospirosis
cytokines
cellular immunity
topic leptospirosis
cytokines
cellular immunity
description Immune response to leptospirosis is mainly humorally mediated, and involves opsonization of leptospires for phagocytosis by macrophages and neutrophils. However, some aspects are still unknown. For a more detailed analysis of the cellular immune response to leptospirosis infection, trials were carried out in order to determine the hydrogen peroxide and nitric oxide (H2O2 and NO) production stimulated or not by Interferon-gamma. The participation of some specific cytokines, such as Tumor Necrosis Factor-alpha (TNF-alpha); Interferon-gamma (IFN-gamma); Interleukin-6 (IL-6); and Interleukin-4 (IL-4), in the immunopathology of this infection was also investigated. For this purpose, we analyzed the supernatant from peritoneal macrophage cell culture and the splenic cells of mice genetically selected as High (H) and Low (L) antibody producers, and inbred Balb/c mice infected with Leptospira interrogans serovar icterohaemorrhagiae. The IL-6 production varied from release peaks to inhibition in H, L, and Balb/c mice. Similar behavior was observed for IL-4, produced only by H and Balb/c mice. The three strains presented constant and elevated production of TNF-alpha until day 14, suggesting its effective participation in the initial phase of the infection. Meanwhile, all the three strains presented a constant and irregular IFN-gamma production, with release peaks between the 7th and 14th days in L mice. The H and Balb/c mice strains presented a higher tendency to Th2 response pattern, whereas L mice tended towards Th1 response.
publishDate 2005
dc.date.none.fl_str_mv 2005-06
2021-07-14T10:30:42Z
2021-07-14T10:30:42Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/S1678-91992005000200009
Journal of Venomous Animals and Toxins including Tropical Diseases. Botucatu, SP, Brazil: Centro de Estudos de Venenos e Animais Peçonhentos, v. 11, n. 2, p. 198-212, 2005.
1678-9199
http://hdl.handle.net/11449/211855
10.1590/S1678-91992005000200009
S1678-91992005000200009
S1678-91992005000200009.pdf
url http://dx.doi.org/10.1590/S1678-91992005000200009
http://hdl.handle.net/11449/211855
identifier_str_mv Journal of Venomous Animals and Toxins including Tropical Diseases. Botucatu, SP, Brazil: Centro de Estudos de Venenos e Animais Peçonhentos, v. 11, n. 2, p. 198-212, 2005.
1678-9199
10.1590/S1678-91992005000200009
S1678-91992005000200009
S1678-91992005000200009.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Journal of Venomous Animals and Toxins including Tropical Diseases
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 198-212
application/pdf
dc.publisher.none.fl_str_mv Centro de Estudos de Venenos e Animais Peçonhentos
publisher.none.fl_str_mv Centro de Estudos de Venenos e Animais Peçonhentos
dc.source.none.fl_str_mv SciELO
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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