Photodynamic inactivation of a multispecies biofilm using curcumin and LED light
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1007/s10103-016-1942-7 http://hdl.handle.net/11449/172879 |
Resumo: | This study evaluated the potential of curcumin-mediated antimicrobial photodynamic inactivation (API) on multispecies biofilms of Candida albicans, Candida glabrata, and Streptococcus mutans of different ages. Acrylic samples (n = 480) were made with standardized rough surfaces and incubated with bacteria and yeast for 24 or 48 h. API was performed with curcumin (80, 100, 120 μM) and LED light. Additional acrylic samples were treated with curcumin or LED light only. Positive control samples received neither light nor curcumin. After API, colony counts were quantified (CFU/mL), cell metabolism was determined by means of XTT assay, and the total biofilm biomass was evaluated using Crystal Violet (CV) staining assay and images were obtained by confocal laser scanning microscopy (CLSM). The data were analyzed by nonparametric two-way ANOVA and post hoc Tukey tests (α < 0.05). For 24-h biofilm, API resulted in statistically significant difference (ρ < 0.001) of viability of C. albicans compared with control (P−L−) for all Cur concentrations. For 48-h biofilm, API resulted in statistically significant difference (ρ < 0.001) compared with control only when Cur at 120 μM was used. API promoted statistically significant difference (ρ ≤ 0.001) in the viability of S. mutans and C. glabrata for all Cur concentrations in the two biofilm ages. In addition, API produced a statistically significant difference (ρ < 0.001) of metabolic activity and of total biomass (ρ < 0.001) of multispecies biofilms compared with control for all Cur concentrations. It can be concluded that both 24- and 48-h biofilms were susceptible to API mediated by Cur; however, 24-h biofilm was more sensitive than the 48-h biofilm. |
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Repositório Institucional da UNESP |
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Photodynamic inactivation of a multispecies biofilm using curcumin and LED lightBacteriaFungiMultispecies biofilmPhotodynamic inactivationThis study evaluated the potential of curcumin-mediated antimicrobial photodynamic inactivation (API) on multispecies biofilms of Candida albicans, Candida glabrata, and Streptococcus mutans of different ages. Acrylic samples (n = 480) were made with standardized rough surfaces and incubated with bacteria and yeast for 24 or 48 h. API was performed with curcumin (80, 100, 120 μM) and LED light. Additional acrylic samples were treated with curcumin or LED light only. Positive control samples received neither light nor curcumin. After API, colony counts were quantified (CFU/mL), cell metabolism was determined by means of XTT assay, and the total biofilm biomass was evaluated using Crystal Violet (CV) staining assay and images were obtained by confocal laser scanning microscopy (CLSM). The data were analyzed by nonparametric two-way ANOVA and post hoc Tukey tests (α < 0.05). For 24-h biofilm, API resulted in statistically significant difference (ρ < 0.001) of viability of C. albicans compared with control (P−L−) for all Cur concentrations. For 48-h biofilm, API resulted in statistically significant difference (ρ < 0.001) compared with control only when Cur at 120 μM was used. API promoted statistically significant difference (ρ ≤ 0.001) in the viability of S. mutans and C. glabrata for all Cur concentrations in the two biofilm ages. In addition, API produced a statistically significant difference (ρ < 0.001) of metabolic activity and of total biomass (ρ < 0.001) of multispecies biofilms compared with control for all Cur concentrations. It can be concluded that both 24- and 48-h biofilms were susceptible to API mediated by Cur; however, 24-h biofilm was more sensitive than the 48-h biofilm.Department of Dental Materials and Prosthodontics Institute of Science and Technology UNESP Univ Estadual Paulista, São José dos Campos, School of Dentistry – Avenida Engenheiro Francisco José Longo 777Department of Dental Materials and Prosthodontics Araraquara Dental School UNESP Univ Estadual Paulista, Rua Humaitá 1680Physics Institute of São Carlos USP University of São Paulo, Av. Trabalhador São-Carlense, 400Department of Dental Materials and Prosthodontics Institute of Science and Technology UNESP Univ Estadual Paulista, São José dos Campos, School of Dentistry – Avenida Engenheiro Francisco José Longo 777Department of Dental Materials and Prosthodontics Araraquara Dental School UNESP Univ Estadual Paulista, Rua Humaitá 1680Universidade Estadual Paulista (Unesp)Universidade de São Paulo (USP)Quishida, Cristiane Campos Costa [UNESP]De Oliveira Mima, Ewerton Garcia [UNESP]Jorge, Janaina Habib [UNESP]Vergani, Carlos Eduardo [UNESP]Bagnato, Vanderlei SalvadorPavarina, Ana Cláudia [UNESP]2018-12-11T17:02:32Z2018-12-11T17:02:32Z2016-07-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article997-1009application/pdfhttp://dx.doi.org/10.1007/s10103-016-1942-7Lasers in Medical Science, v. 31, n. 5, p. 997-1009, 2016.1435-604X0268-8921http://hdl.handle.net/11449/17287910.1007/s10103-016-1942-72-s2.0-849645103682-s2.0-84964510368.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengLasers in Medical Science0,713info:eu-repo/semantics/openAccess2023-11-20T06:14:09Zoai:repositorio.unesp.br:11449/172879Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T18:15:39.996053Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Photodynamic inactivation of a multispecies biofilm using curcumin and LED light |
title |
Photodynamic inactivation of a multispecies biofilm using curcumin and LED light |
spellingShingle |
Photodynamic inactivation of a multispecies biofilm using curcumin and LED light Quishida, Cristiane Campos Costa [UNESP] Bacteria Fungi Multispecies biofilm Photodynamic inactivation |
title_short |
Photodynamic inactivation of a multispecies biofilm using curcumin and LED light |
title_full |
Photodynamic inactivation of a multispecies biofilm using curcumin and LED light |
title_fullStr |
Photodynamic inactivation of a multispecies biofilm using curcumin and LED light |
title_full_unstemmed |
Photodynamic inactivation of a multispecies biofilm using curcumin and LED light |
title_sort |
Photodynamic inactivation of a multispecies biofilm using curcumin and LED light |
author |
Quishida, Cristiane Campos Costa [UNESP] |
author_facet |
Quishida, Cristiane Campos Costa [UNESP] De Oliveira Mima, Ewerton Garcia [UNESP] Jorge, Janaina Habib [UNESP] Vergani, Carlos Eduardo [UNESP] Bagnato, Vanderlei Salvador Pavarina, Ana Cláudia [UNESP] |
author_role |
author |
author2 |
De Oliveira Mima, Ewerton Garcia [UNESP] Jorge, Janaina Habib [UNESP] Vergani, Carlos Eduardo [UNESP] Bagnato, Vanderlei Salvador Pavarina, Ana Cláudia [UNESP] |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
Quishida, Cristiane Campos Costa [UNESP] De Oliveira Mima, Ewerton Garcia [UNESP] Jorge, Janaina Habib [UNESP] Vergani, Carlos Eduardo [UNESP] Bagnato, Vanderlei Salvador Pavarina, Ana Cláudia [UNESP] |
dc.subject.por.fl_str_mv |
Bacteria Fungi Multispecies biofilm Photodynamic inactivation |
topic |
Bacteria Fungi Multispecies biofilm Photodynamic inactivation |
description |
This study evaluated the potential of curcumin-mediated antimicrobial photodynamic inactivation (API) on multispecies biofilms of Candida albicans, Candida glabrata, and Streptococcus mutans of different ages. Acrylic samples (n = 480) were made with standardized rough surfaces and incubated with bacteria and yeast for 24 or 48 h. API was performed with curcumin (80, 100, 120 μM) and LED light. Additional acrylic samples were treated with curcumin or LED light only. Positive control samples received neither light nor curcumin. After API, colony counts were quantified (CFU/mL), cell metabolism was determined by means of XTT assay, and the total biofilm biomass was evaluated using Crystal Violet (CV) staining assay and images were obtained by confocal laser scanning microscopy (CLSM). The data were analyzed by nonparametric two-way ANOVA and post hoc Tukey tests (α < 0.05). For 24-h biofilm, API resulted in statistically significant difference (ρ < 0.001) of viability of C. albicans compared with control (P−L−) for all Cur concentrations. For 48-h biofilm, API resulted in statistically significant difference (ρ < 0.001) compared with control only when Cur at 120 μM was used. API promoted statistically significant difference (ρ ≤ 0.001) in the viability of S. mutans and C. glabrata for all Cur concentrations in the two biofilm ages. In addition, API produced a statistically significant difference (ρ < 0.001) of metabolic activity and of total biomass (ρ < 0.001) of multispecies biofilms compared with control for all Cur concentrations. It can be concluded that both 24- and 48-h biofilms were susceptible to API mediated by Cur; however, 24-h biofilm was more sensitive than the 48-h biofilm. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-07-01 2018-12-11T17:02:32Z 2018-12-11T17:02:32Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/s10103-016-1942-7 Lasers in Medical Science, v. 31, n. 5, p. 997-1009, 2016. 1435-604X 0268-8921 http://hdl.handle.net/11449/172879 10.1007/s10103-016-1942-7 2-s2.0-84964510368 2-s2.0-84964510368.pdf |
url |
http://dx.doi.org/10.1007/s10103-016-1942-7 http://hdl.handle.net/11449/172879 |
identifier_str_mv |
Lasers in Medical Science, v. 31, n. 5, p. 997-1009, 2016. 1435-604X 0268-8921 10.1007/s10103-016-1942-7 2-s2.0-84964510368 2-s2.0-84964510368.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Lasers in Medical Science 0,713 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
997-1009 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128911842738176 |