Composition and leishmanicidal activity of the essential oil of Vernonia polyanthes Less (Asteraceae)
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1080/14786419.2017.1299723 http://hdl.handle.net/11449/174424 |
Resumo: | The composition of the essential oil (EO) from leaves of Vernonia polyanthes and the evaluation of its leishmanicidal potential are reported here for the first time. The oil obtained by hydrodistillation was analysed by combination of GC and GC/MS. Thirty-five compounds were identified, representing 91.8% of the oil composition. The oil consists primarily of monoterpenes (37.1%), sesquiterpenes (26.3%) and oxygenated sesquiterpenes (23.9%), myrcene (34.3%), zerumbone (15.8%), bicyclogermacrene (8.9%), α-humulene (4.8%) and germacrene D (4.3%) being the major constituents. Activity against Leishmania infantum was determined using the tetrazolium dye (MTT) colorimetric method. The oil, as well as zerumbone, one of its major constituents, showed significant leishmanicidal activity, with IC50 values of 19.4 and 9.0 μg/ml, respectively. Cytotoxicity in macrophages cells was evaluated using the MTT colorimetric assay. The EO showed the CC50 < 10 μg/ml to macrophages cells. |
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Composition and leishmanicidal activity of the essential oil of Vernonia polyanthes Less (Asteraceae)essential oilLeishmania infantumleishmanicidal activityVernonia polyantheszerumboneThe composition of the essential oil (EO) from leaves of Vernonia polyanthes and the evaluation of its leishmanicidal potential are reported here for the first time. The oil obtained by hydrodistillation was analysed by combination of GC and GC/MS. Thirty-five compounds were identified, representing 91.8% of the oil composition. The oil consists primarily of monoterpenes (37.1%), sesquiterpenes (26.3%) and oxygenated sesquiterpenes (23.9%), myrcene (34.3%), zerumbone (15.8%), bicyclogermacrene (8.9%), α-humulene (4.8%) and germacrene D (4.3%) being the major constituents. Activity against Leishmania infantum was determined using the tetrazolium dye (MTT) colorimetric method. The oil, as well as zerumbone, one of its major constituents, showed significant leishmanicidal activity, with IC50 values of 19.4 and 9.0 μg/ml, respectively. Cytotoxicity in macrophages cells was evaluated using the MTT colorimetric assay. The EO showed the CC50 < 10 μg/ml to macrophages cells.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Faculdade de Ciências Farmacêuticas Departamento de Princípios Ativos Naturais e Toxicologia Universidade Estadual Paulista (UNESP)Faculdade de Farmácia Fundação Educacional de Barretos UNIFEBFaculdade de Farmácia Universidade Estadual de MaringáCentro de Neurociências e Biologia Celular Universidade de CoimbraFaculdade de Farmácia Universidade de CoimbraFaculdade de Ciências Farmacêuticas Departamento de Princípios Ativos Naturais e Toxicologia Universidade Estadual Paulista (UNESP)Universidade Estadual Paulista (Unesp)Fundação Educacional de Barretos UNIFEBUniversidade Estadual de Maringá (UEM)Universidade de CoimbraMoreira, Raquel Regina Duarte [UNESP]Martins, Gilmarcio ZimmermannVarandas, RaquelCogo, JulianaPerego, Caio Humberto [UNESP]Roncoli, GiovanaSousa, Maria do CéuNakamura, Celso VataruSalgueiro, LígiaCavaleiro, Carlos2018-12-11T17:11:03Z2018-12-11T17:11:03Z2017-12-17info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article2905-2908application/pdfhttp://dx.doi.org/10.1080/14786419.2017.1299723Natural Product Research, v. 31, n. 24, p. 2905-2908, 2017.1478-64271478-6419http://hdl.handle.net/11449/17442410.1080/14786419.2017.12997232-s2.0-850169613582-s2.0-85016961358.pdf77103444618649240000-0002-4253-0320Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengNatural Product Research0,671info:eu-repo/semantics/openAccess2024-06-24T14:51:53Zoai:repositorio.unesp.br:11449/174424Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T21:00:30.390903Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Composition and leishmanicidal activity of the essential oil of Vernonia polyanthes Less (Asteraceae) |
title |
Composition and leishmanicidal activity of the essential oil of Vernonia polyanthes Less (Asteraceae) |
spellingShingle |
Composition and leishmanicidal activity of the essential oil of Vernonia polyanthes Less (Asteraceae) Moreira, Raquel Regina Duarte [UNESP] essential oil Leishmania infantum leishmanicidal activity Vernonia polyanthes zerumbone |
title_short |
Composition and leishmanicidal activity of the essential oil of Vernonia polyanthes Less (Asteraceae) |
title_full |
Composition and leishmanicidal activity of the essential oil of Vernonia polyanthes Less (Asteraceae) |
title_fullStr |
Composition and leishmanicidal activity of the essential oil of Vernonia polyanthes Less (Asteraceae) |
title_full_unstemmed |
Composition and leishmanicidal activity of the essential oil of Vernonia polyanthes Less (Asteraceae) |
title_sort |
Composition and leishmanicidal activity of the essential oil of Vernonia polyanthes Less (Asteraceae) |
author |
Moreira, Raquel Regina Duarte [UNESP] |
author_facet |
Moreira, Raquel Regina Duarte [UNESP] Martins, Gilmarcio Zimmermann Varandas, Raquel Cogo, Juliana Perego, Caio Humberto [UNESP] Roncoli, Giovana Sousa, Maria do Céu Nakamura, Celso Vataru Salgueiro, Lígia Cavaleiro, Carlos |
author_role |
author |
author2 |
Martins, Gilmarcio Zimmermann Varandas, Raquel Cogo, Juliana Perego, Caio Humberto [UNESP] Roncoli, Giovana Sousa, Maria do Céu Nakamura, Celso Vataru Salgueiro, Lígia Cavaleiro, Carlos |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Fundação Educacional de Barretos UNIFEB Universidade Estadual de Maringá (UEM) Universidade de Coimbra |
dc.contributor.author.fl_str_mv |
Moreira, Raquel Regina Duarte [UNESP] Martins, Gilmarcio Zimmermann Varandas, Raquel Cogo, Juliana Perego, Caio Humberto [UNESP] Roncoli, Giovana Sousa, Maria do Céu Nakamura, Celso Vataru Salgueiro, Lígia Cavaleiro, Carlos |
dc.subject.por.fl_str_mv |
essential oil Leishmania infantum leishmanicidal activity Vernonia polyanthes zerumbone |
topic |
essential oil Leishmania infantum leishmanicidal activity Vernonia polyanthes zerumbone |
description |
The composition of the essential oil (EO) from leaves of Vernonia polyanthes and the evaluation of its leishmanicidal potential are reported here for the first time. The oil obtained by hydrodistillation was analysed by combination of GC and GC/MS. Thirty-five compounds were identified, representing 91.8% of the oil composition. The oil consists primarily of monoterpenes (37.1%), sesquiterpenes (26.3%) and oxygenated sesquiterpenes (23.9%), myrcene (34.3%), zerumbone (15.8%), bicyclogermacrene (8.9%), α-humulene (4.8%) and germacrene D (4.3%) being the major constituents. Activity against Leishmania infantum was determined using the tetrazolium dye (MTT) colorimetric method. The oil, as well as zerumbone, one of its major constituents, showed significant leishmanicidal activity, with IC50 values of 19.4 and 9.0 μg/ml, respectively. Cytotoxicity in macrophages cells was evaluated using the MTT colorimetric assay. The EO showed the CC50 < 10 μg/ml to macrophages cells. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-12-17 2018-12-11T17:11:03Z 2018-12-11T17:11:03Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1080/14786419.2017.1299723 Natural Product Research, v. 31, n. 24, p. 2905-2908, 2017. 1478-6427 1478-6419 http://hdl.handle.net/11449/174424 10.1080/14786419.2017.1299723 2-s2.0-85016961358 2-s2.0-85016961358.pdf 7710344461864924 0000-0002-4253-0320 |
url |
http://dx.doi.org/10.1080/14786419.2017.1299723 http://hdl.handle.net/11449/174424 |
identifier_str_mv |
Natural Product Research, v. 31, n. 24, p. 2905-2908, 2017. 1478-6427 1478-6419 10.1080/14786419.2017.1299723 2-s2.0-85016961358 2-s2.0-85016961358.pdf 7710344461864924 0000-0002-4253-0320 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Natural Product Research 0,671 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
2905-2908 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1808129272817123328 |