Deprivation of dietary fiber enhances susceptibility of mice to cryptosporidiosis

Detalhes bibliográficos
Autor(a) principal: Oliveira, Bruno César Miranda [UNESP]
Data de Publicação: 2019
Outros Autores: Bresciani, Katia Denise Saraiva [UNESP], Widmer, Giovanni
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1371/journal.pntd.0007411
http://hdl.handle.net/11449/201214
Resumo: Based on our initial observations showing that mice consuming a probiotic product develop more severe cryptosporidiosis, we investigated the impact of other dietary interventions on the intracellular proliferation of Cryptosporidium parvum and C. tyzzeri in the mouse. Mice were orally infected with oocysts and parasite multiplication measured by quantifying fecal oocyst output. High-throughput sequencing of 16S ribosomal RNA amplicons was used to correlate oocyst output with diet and with the composition of the intestinal microbiota. On average, mice fed a diet without fiber (cellulose, pectin and inulin) developed more severe infections. As expected, a diet without fibers also significantly altered the fecal microbiota. Consistent with these observations, mice fed a prebiotic product sold for human consumption excreted significantly fewer oocysts. The fecal microbiota of mice consuming no plant polysaccharides was characterized by a lower relative abundance of Bacteroidetes bacteria. Since bacterial metabolites play an important role in the physiology of intestinal enterocytes, we hypothesize based on these observations that the impact of diet on parasite proliferation is mediated primarily by the metabolic activity of the anaerobic microbiota, specifically by the effect of certain metabolites on the host. This model is consistent with the metabolic dependence of intracellular stages of the parasite on the host cell. These observations underscore the potential of dietary interventions to alleviate the impact of cryptosporidiosis, particularly in infants at risk of recurrent enteric infections.
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spelling Deprivation of dietary fiber enhances susceptibility of mice to cryptosporidiosisBased on our initial observations showing that mice consuming a probiotic product develop more severe cryptosporidiosis, we investigated the impact of other dietary interventions on the intracellular proliferation of Cryptosporidium parvum and C. tyzzeri in the mouse. Mice were orally infected with oocysts and parasite multiplication measured by quantifying fecal oocyst output. High-throughput sequencing of 16S ribosomal RNA amplicons was used to correlate oocyst output with diet and with the composition of the intestinal microbiota. On average, mice fed a diet without fiber (cellulose, pectin and inulin) developed more severe infections. As expected, a diet without fibers also significantly altered the fecal microbiota. Consistent with these observations, mice fed a prebiotic product sold for human consumption excreted significantly fewer oocysts. The fecal microbiota of mice consuming no plant polysaccharides was characterized by a lower relative abundance of Bacteroidetes bacteria. Since bacterial metabolites play an important role in the physiology of intestinal enterocytes, we hypothesize based on these observations that the impact of diet on parasite proliferation is mediated primarily by the metabolic activity of the anaerobic microbiota, specifically by the effect of certain metabolites on the host. This model is consistent with the metabolic dependence of intracellular stages of the parasite on the host cell. These observations underscore the potential of dietary interventions to alleviate the impact of cryptosporidiosis, particularly in infants at risk of recurrent enteric infections.Cummings School of Veterinary Medicine Tufts UniversityUniversidade Estadual Paulista (Unesp) Faculdade de Medicina VeterináriaUniversidade Estadual Paulista (Unesp) Faculdade de Medicina VeterináriaTufts UniversityUniversidade Estadual Paulista (Unesp)Oliveira, Bruno César Miranda [UNESP]Bresciani, Katia Denise Saraiva [UNESP]Widmer, Giovanni2020-12-12T02:27:00Z2020-12-12T02:27:00Z2019-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1371/journal.pntd.0007411PLoS Neglected Tropical Diseases, v. 13, n. 9, 2019.1935-27351935-2727http://hdl.handle.net/11449/20121410.1371/journal.pntd.00074112-s2.0-85073125137Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPLoS Neglected Tropical Diseasesinfo:eu-repo/semantics/openAccess2024-09-04T19:15:11Zoai:repositorio.unesp.br:11449/201214Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-04T19:15:11Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Deprivation of dietary fiber enhances susceptibility of mice to cryptosporidiosis
title Deprivation of dietary fiber enhances susceptibility of mice to cryptosporidiosis
spellingShingle Deprivation of dietary fiber enhances susceptibility of mice to cryptosporidiosis
Oliveira, Bruno César Miranda [UNESP]
title_short Deprivation of dietary fiber enhances susceptibility of mice to cryptosporidiosis
title_full Deprivation of dietary fiber enhances susceptibility of mice to cryptosporidiosis
title_fullStr Deprivation of dietary fiber enhances susceptibility of mice to cryptosporidiosis
title_full_unstemmed Deprivation of dietary fiber enhances susceptibility of mice to cryptosporidiosis
title_sort Deprivation of dietary fiber enhances susceptibility of mice to cryptosporidiosis
author Oliveira, Bruno César Miranda [UNESP]
author_facet Oliveira, Bruno César Miranda [UNESP]
Bresciani, Katia Denise Saraiva [UNESP]
Widmer, Giovanni
author_role author
author2 Bresciani, Katia Denise Saraiva [UNESP]
Widmer, Giovanni
author2_role author
author
dc.contributor.none.fl_str_mv Tufts University
Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Oliveira, Bruno César Miranda [UNESP]
Bresciani, Katia Denise Saraiva [UNESP]
Widmer, Giovanni
description Based on our initial observations showing that mice consuming a probiotic product develop more severe cryptosporidiosis, we investigated the impact of other dietary interventions on the intracellular proliferation of Cryptosporidium parvum and C. tyzzeri in the mouse. Mice were orally infected with oocysts and parasite multiplication measured by quantifying fecal oocyst output. High-throughput sequencing of 16S ribosomal RNA amplicons was used to correlate oocyst output with diet and with the composition of the intestinal microbiota. On average, mice fed a diet without fiber (cellulose, pectin and inulin) developed more severe infections. As expected, a diet without fibers also significantly altered the fecal microbiota. Consistent with these observations, mice fed a prebiotic product sold for human consumption excreted significantly fewer oocysts. The fecal microbiota of mice consuming no plant polysaccharides was characterized by a lower relative abundance of Bacteroidetes bacteria. Since bacterial metabolites play an important role in the physiology of intestinal enterocytes, we hypothesize based on these observations that the impact of diet on parasite proliferation is mediated primarily by the metabolic activity of the anaerobic microbiota, specifically by the effect of certain metabolites on the host. This model is consistent with the metabolic dependence of intracellular stages of the parasite on the host cell. These observations underscore the potential of dietary interventions to alleviate the impact of cryptosporidiosis, particularly in infants at risk of recurrent enteric infections.
publishDate 2019
dc.date.none.fl_str_mv 2019-01-01
2020-12-12T02:27:00Z
2020-12-12T02:27:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1371/journal.pntd.0007411
PLoS Neglected Tropical Diseases, v. 13, n. 9, 2019.
1935-2735
1935-2727
http://hdl.handle.net/11449/201214
10.1371/journal.pntd.0007411
2-s2.0-85073125137
url http://dx.doi.org/10.1371/journal.pntd.0007411
http://hdl.handle.net/11449/201214
identifier_str_mv PLoS Neglected Tropical Diseases, v. 13, n. 9, 2019.
1935-2735
1935-2727
10.1371/journal.pntd.0007411
2-s2.0-85073125137
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv PLoS Neglected Tropical Diseases
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv repositoriounesp@unesp.br
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