Application of Autologous Platelet-Rich Plasma on Tooth Extraction Site Prevents Occurence of Medication-Related Osteonecrosis of the Jaws in Rats
Autor(a) principal: | |
---|---|
Data de Publicação: | 2019 |
Outros Autores: | , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1038/s41598-018-37063-y http://hdl.handle.net/11449/190032 |
Resumo: | This study evaluated the effects of local application of autologous platelet-rich plasma (PRP) on the tooth extraction site of rats presenting the main risk factors for medication-related osteonecrosis of the jaw (MRONJ). For seven weeks, senile rats were submitted to systemic treatment with vehicle (VEH and VEH-PRP) or 100 μg/Kg of zoledronate (ZOL and ZOL-PRP) every three days. After three weeks, the first lower molar was extracted. VEH-PRP and ZOL-PRP received PRP at the tooth extraction site. Euthanasia was performed at 28 days postoperatively. Clinical, histopathological, histometric and immunohistochemical analyses were carried out in histological sections from the tooth extraction site. ZOL showed lower percentage of newly formed bone tissue (NFBT), higher percentage of non-vital bone tissue (NVBT), as well as higher immunolabeling for TNFα and IL-1β. In addition, ZOL presented lower immunolabeling for PCNA, VEGF, BMP2/4, OCN and TRAP. VEH and ZOL-PRP showed improvement in the tooth extraction site wound healing and comparable percentage of NFBT, VEGF, BMP2/4 and OCN. Local application of autologous PRP proved a viable preventive therapy, which is safe and effective to restore tissue repair capacity of the tooth extraction site and prevent the occurrence of MRONJ following tooth extraction. |
id |
UNSP_f1f7cf1f4d80c3e77cd4a959c2bf8483 |
---|---|
oai_identifier_str |
oai:repositorio.unesp.br:11449/190032 |
network_acronym_str |
UNSP |
network_name_str |
Repositório Institucional da UNESP |
repository_id_str |
2946 |
spelling |
Application of Autologous Platelet-Rich Plasma on Tooth Extraction Site Prevents Occurence of Medication-Related Osteonecrosis of the Jaws in RatsThis study evaluated the effects of local application of autologous platelet-rich plasma (PRP) on the tooth extraction site of rats presenting the main risk factors for medication-related osteonecrosis of the jaw (MRONJ). For seven weeks, senile rats were submitted to systemic treatment with vehicle (VEH and VEH-PRP) or 100 μg/Kg of zoledronate (ZOL and ZOL-PRP) every three days. After three weeks, the first lower molar was extracted. VEH-PRP and ZOL-PRP received PRP at the tooth extraction site. Euthanasia was performed at 28 days postoperatively. Clinical, histopathological, histometric and immunohistochemical analyses were carried out in histological sections from the tooth extraction site. ZOL showed lower percentage of newly formed bone tissue (NFBT), higher percentage of non-vital bone tissue (NVBT), as well as higher immunolabeling for TNFα and IL-1β. In addition, ZOL presented lower immunolabeling for PCNA, VEGF, BMP2/4, OCN and TRAP. VEH and ZOL-PRP showed improvement in the tooth extraction site wound healing and comparable percentage of NFBT, VEGF, BMP2/4 and OCN. Local application of autologous PRP proved a viable preventive therapy, which is safe and effective to restore tissue repair capacity of the tooth extraction site and prevent the occurrence of MRONJ following tooth extraction.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)São Paulo State University (UNESP) School of Dentistry Department of Basic Sciences Rua José Bonifácio 1193 CEPSão Paulo State University (UNESP) Institute of Biosciences Rua Prof. Dr. Antônio Celso Wagner Zanin 250 CEPSão Paulo State University (UNESP) School of Dentistry Department of Surgery and Integrated Clinic Rua José Bonifácio 1193 CEPSão Paulo State University (UNESP) School of Dentistry Department of Restorative Dentistry Rua José Bonifácio 1193 CEPSão Paulo University (USP) School of Dentistry Department of Morphology Physiology and Basic Pathology Avenida do Café s/n CEPSão Paulo State University (UNESP) School of Dentistry Department of Basic Sciences Rua José Bonifácio 1193 CEPSão Paulo State University (UNESP) Institute of Biosciences Rua Prof. Dr. Antônio Celso Wagner Zanin 250 CEPSão Paulo State University (UNESP) School of Dentistry Department of Surgery and Integrated Clinic Rua José Bonifácio 1193 CEPSão Paulo State University (UNESP) School of Dentistry Department of Restorative Dentistry Rua José Bonifácio 1193 CEPFAPESP: 2013/26779-4Universidade Estadual Paulista (Unesp)Universidade de São Paulo (USP)Toro, Luan Felipe [UNESP]de Mello-Neto, João Martins [UNESP]Santos, Fernanda Furuse Ventura dos [UNESP]Ferreira, Letícia Chaves [UNESP]Statkievicz, Cristian [UNESP]Cintra, Luciano Tavares Ângelo [UNESP]Issa, João Paulo MardeganDornelles, Rita Cássia Menegati [UNESP]de Almeida, Juliano Milanezi [UNESP]Nagata, Maria José Hitomi [UNESP]Garcia, Valdir Gouveia [UNESP]Theodoro, Leticia Helena [UNESP]Casatti, Cláudio Aparecido [UNESP]Ervolino, Edilson [UNESP]2019-10-06T17:00:02Z2019-10-06T17:00:02Z2019-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1038/s41598-018-37063-yScientific Reports, v. 9, n. 1, 2019.2045-2322http://hdl.handle.net/11449/19003210.1038/s41598-018-37063-y2-s2.0-8505982919544080955173468468399870097572073543590242278488984874626269318770000-0003-4859-05830000-0003-0783-66120000-0001-5650-7343Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengScientific Reportsinfo:eu-repo/semantics/openAccess2021-12-15T10:34:18Zoai:repositorio.unesp.br:11449/190032Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-12-15T10:34:18Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Application of Autologous Platelet-Rich Plasma on Tooth Extraction Site Prevents Occurence of Medication-Related Osteonecrosis of the Jaws in Rats |
title |
Application of Autologous Platelet-Rich Plasma on Tooth Extraction Site Prevents Occurence of Medication-Related Osteonecrosis of the Jaws in Rats |
spellingShingle |
Application of Autologous Platelet-Rich Plasma on Tooth Extraction Site Prevents Occurence of Medication-Related Osteonecrosis of the Jaws in Rats Toro, Luan Felipe [UNESP] |
title_short |
Application of Autologous Platelet-Rich Plasma on Tooth Extraction Site Prevents Occurence of Medication-Related Osteonecrosis of the Jaws in Rats |
title_full |
Application of Autologous Platelet-Rich Plasma on Tooth Extraction Site Prevents Occurence of Medication-Related Osteonecrosis of the Jaws in Rats |
title_fullStr |
Application of Autologous Platelet-Rich Plasma on Tooth Extraction Site Prevents Occurence of Medication-Related Osteonecrosis of the Jaws in Rats |
title_full_unstemmed |
Application of Autologous Platelet-Rich Plasma on Tooth Extraction Site Prevents Occurence of Medication-Related Osteonecrosis of the Jaws in Rats |
title_sort |
Application of Autologous Platelet-Rich Plasma on Tooth Extraction Site Prevents Occurence of Medication-Related Osteonecrosis of the Jaws in Rats |
author |
Toro, Luan Felipe [UNESP] |
author_facet |
Toro, Luan Felipe [UNESP] de Mello-Neto, João Martins [UNESP] Santos, Fernanda Furuse Ventura dos [UNESP] Ferreira, Letícia Chaves [UNESP] Statkievicz, Cristian [UNESP] Cintra, Luciano Tavares Ângelo [UNESP] Issa, João Paulo Mardegan Dornelles, Rita Cássia Menegati [UNESP] de Almeida, Juliano Milanezi [UNESP] Nagata, Maria José Hitomi [UNESP] Garcia, Valdir Gouveia [UNESP] Theodoro, Leticia Helena [UNESP] Casatti, Cláudio Aparecido [UNESP] Ervolino, Edilson [UNESP] |
author_role |
author |
author2 |
de Mello-Neto, João Martins [UNESP] Santos, Fernanda Furuse Ventura dos [UNESP] Ferreira, Letícia Chaves [UNESP] Statkievicz, Cristian [UNESP] Cintra, Luciano Tavares Ângelo [UNESP] Issa, João Paulo Mardegan Dornelles, Rita Cássia Menegati [UNESP] de Almeida, Juliano Milanezi [UNESP] Nagata, Maria José Hitomi [UNESP] Garcia, Valdir Gouveia [UNESP] Theodoro, Leticia Helena [UNESP] Casatti, Cláudio Aparecido [UNESP] Ervolino, Edilson [UNESP] |
author2_role |
author author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
Toro, Luan Felipe [UNESP] de Mello-Neto, João Martins [UNESP] Santos, Fernanda Furuse Ventura dos [UNESP] Ferreira, Letícia Chaves [UNESP] Statkievicz, Cristian [UNESP] Cintra, Luciano Tavares Ângelo [UNESP] Issa, João Paulo Mardegan Dornelles, Rita Cássia Menegati [UNESP] de Almeida, Juliano Milanezi [UNESP] Nagata, Maria José Hitomi [UNESP] Garcia, Valdir Gouveia [UNESP] Theodoro, Leticia Helena [UNESP] Casatti, Cláudio Aparecido [UNESP] Ervolino, Edilson [UNESP] |
description |
This study evaluated the effects of local application of autologous platelet-rich plasma (PRP) on the tooth extraction site of rats presenting the main risk factors for medication-related osteonecrosis of the jaw (MRONJ). For seven weeks, senile rats were submitted to systemic treatment with vehicle (VEH and VEH-PRP) or 100 μg/Kg of zoledronate (ZOL and ZOL-PRP) every three days. After three weeks, the first lower molar was extracted. VEH-PRP and ZOL-PRP received PRP at the tooth extraction site. Euthanasia was performed at 28 days postoperatively. Clinical, histopathological, histometric and immunohistochemical analyses were carried out in histological sections from the tooth extraction site. ZOL showed lower percentage of newly formed bone tissue (NFBT), higher percentage of non-vital bone tissue (NVBT), as well as higher immunolabeling for TNFα and IL-1β. In addition, ZOL presented lower immunolabeling for PCNA, VEGF, BMP2/4, OCN and TRAP. VEH and ZOL-PRP showed improvement in the tooth extraction site wound healing and comparable percentage of NFBT, VEGF, BMP2/4 and OCN. Local application of autologous PRP proved a viable preventive therapy, which is safe and effective to restore tissue repair capacity of the tooth extraction site and prevent the occurrence of MRONJ following tooth extraction. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-10-06T17:00:02Z 2019-10-06T17:00:02Z 2019-12-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1038/s41598-018-37063-y Scientific Reports, v. 9, n. 1, 2019. 2045-2322 http://hdl.handle.net/11449/190032 10.1038/s41598-018-37063-y 2-s2.0-85059829195 4408095517346846 8399870097572073 5435902422784889 8487462626931877 0000-0003-4859-0583 0000-0003-0783-6612 0000-0001-5650-7343 |
url |
http://dx.doi.org/10.1038/s41598-018-37063-y http://hdl.handle.net/11449/190032 |
identifier_str_mv |
Scientific Reports, v. 9, n. 1, 2019. 2045-2322 10.1038/s41598-018-37063-y 2-s2.0-85059829195 4408095517346846 8399870097572073 5435902422784889 8487462626931877 0000-0003-4859-0583 0000-0003-0783-6612 0000-0001-5650-7343 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Scientific Reports |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799964676205838336 |