Identification of a molecular marker associated with lignotuber in Eucalyptus ssp

Detalhes bibliográficos
Autor(a) principal: Bortoloto, Tânia M. [UNESP]
Data de Publicação: 2020
Outros Autores: Fuchs-Ferraz, Maria C. P. [UNESP], Kettener, Karine [UNESP], Martins Rubio, Lígia [UNESP], González, Esteban R., de Souza, Izabel C. G., Oda, Shinitiro, Rossini, Bruno C. [UNESP], Marino, Celso L. [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1038/s41598-020-60308-8
http://hdl.handle.net/11449/198588
Resumo: About 95% of Eucalyptus species present an organ known as a lignotuber, a basal woody swelling that holds a large number of dormant buds in a protected position along with carbohydrates and other nutrients. The importance of this trait in Eucalyptus species relates to its regenerative capacity, particularly in the context of coppicing practices and survival in regions of high abiotic stress, especially fire. In this study, we identified and characterized a genomic region associated with the lignotuber trait in commercially important Eucalyptus species by developing a polymorphic marker that co-segregates with lignotuber presence. The marker was then converted into a SCAR (Sequence Characterized Amplified Region) marker, validated in four other Eucalyptus species and hybrids and analyzed in silico. Our investigation presents a marker (ELig) that is effective in identifying individuals with lignotuber. In silico and Southern blot analyses show that the marker is present in a single copy region and is related to auxilin/cyclin-G associated kinase, containing a DnaJ domain. The ELig marker is an important tool that can be used to manage crosses in Eucalyptus breeding programs and inform studies involving lignotuber development and genetics.
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spelling Identification of a molecular marker associated with lignotuber in Eucalyptus sspAbout 95% of Eucalyptus species present an organ known as a lignotuber, a basal woody swelling that holds a large number of dormant buds in a protected position along with carbohydrates and other nutrients. The importance of this trait in Eucalyptus species relates to its regenerative capacity, particularly in the context of coppicing practices and survival in regions of high abiotic stress, especially fire. In this study, we identified and characterized a genomic region associated with the lignotuber trait in commercially important Eucalyptus species by developing a polymorphic marker that co-segregates with lignotuber presence. The marker was then converted into a SCAR (Sequence Characterized Amplified Region) marker, validated in four other Eucalyptus species and hybrids and analyzed in silico. Our investigation presents a marker (ELig) that is effective in identifying individuals with lignotuber. In silico and Southern blot analyses show that the marker is present in a single copy region and is related to auxilin/cyclin-G associated kinase, containing a DnaJ domain. The ELig marker is an important tool that can be used to manage crosses in Eucalyptus breeding programs and inform studies involving lignotuber development and genetics.Departamento de Genética Instituto de Biociências UNESP - Univ Estadual Paulista R. Prof. Dr. Antônio Celso Wagner Zanin s/nSuzano Papel e Celulose SA Av. Dr. José Lembo 1010Instituto de Biotecnologia (IBTEC) UNESP - Univ Estadual Paulista Alameda das Tecomarias s/nDepartamento de Genética Instituto de Biociências UNESP - Univ Estadual Paulista R. Prof. Dr. Antônio Celso Wagner Zanin s/nInstituto de Biotecnologia (IBTEC) UNESP - Univ Estadual Paulista Alameda das Tecomarias s/nUniversidade Estadual Paulista (Unesp)Av. Dr. José Lembo 1010Bortoloto, Tânia M. [UNESP]Fuchs-Ferraz, Maria C. P. [UNESP]Kettener, Karine [UNESP]Martins Rubio, Lígia [UNESP]González, Esteban R.de Souza, Izabel C. G.Oda, ShinitiroRossini, Bruno C. [UNESP]Marino, Celso L. [UNESP]2020-12-12T01:16:54Z2020-12-12T01:16:54Z2020-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1038/s41598-020-60308-8Scientific Reports, v. 10, n. 1, 2020.2045-2322http://hdl.handle.net/11449/19858810.1038/s41598-020-60308-82-s2.0-8508097209201653487382083190000-0003-4524-954XScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengScientific Reportsinfo:eu-repo/semantics/openAccess2021-10-22T17:11:42Zoai:repositorio.unesp.br:11449/198588Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T17:06:07.763703Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Identification of a molecular marker associated with lignotuber in Eucalyptus ssp
title Identification of a molecular marker associated with lignotuber in Eucalyptus ssp
spellingShingle Identification of a molecular marker associated with lignotuber in Eucalyptus ssp
Bortoloto, Tânia M. [UNESP]
title_short Identification of a molecular marker associated with lignotuber in Eucalyptus ssp
title_full Identification of a molecular marker associated with lignotuber in Eucalyptus ssp
title_fullStr Identification of a molecular marker associated with lignotuber in Eucalyptus ssp
title_full_unstemmed Identification of a molecular marker associated with lignotuber in Eucalyptus ssp
title_sort Identification of a molecular marker associated with lignotuber in Eucalyptus ssp
author Bortoloto, Tânia M. [UNESP]
author_facet Bortoloto, Tânia M. [UNESP]
Fuchs-Ferraz, Maria C. P. [UNESP]
Kettener, Karine [UNESP]
Martins Rubio, Lígia [UNESP]
González, Esteban R.
de Souza, Izabel C. G.
Oda, Shinitiro
Rossini, Bruno C. [UNESP]
Marino, Celso L. [UNESP]
author_role author
author2 Fuchs-Ferraz, Maria C. P. [UNESP]
Kettener, Karine [UNESP]
Martins Rubio, Lígia [UNESP]
González, Esteban R.
de Souza, Izabel C. G.
Oda, Shinitiro
Rossini, Bruno C. [UNESP]
Marino, Celso L. [UNESP]
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Av. Dr. José Lembo 1010
dc.contributor.author.fl_str_mv Bortoloto, Tânia M. [UNESP]
Fuchs-Ferraz, Maria C. P. [UNESP]
Kettener, Karine [UNESP]
Martins Rubio, Lígia [UNESP]
González, Esteban R.
de Souza, Izabel C. G.
Oda, Shinitiro
Rossini, Bruno C. [UNESP]
Marino, Celso L. [UNESP]
description About 95% of Eucalyptus species present an organ known as a lignotuber, a basal woody swelling that holds a large number of dormant buds in a protected position along with carbohydrates and other nutrients. The importance of this trait in Eucalyptus species relates to its regenerative capacity, particularly in the context of coppicing practices and survival in regions of high abiotic stress, especially fire. In this study, we identified and characterized a genomic region associated with the lignotuber trait in commercially important Eucalyptus species by developing a polymorphic marker that co-segregates with lignotuber presence. The marker was then converted into a SCAR (Sequence Characterized Amplified Region) marker, validated in four other Eucalyptus species and hybrids and analyzed in silico. Our investigation presents a marker (ELig) that is effective in identifying individuals with lignotuber. In silico and Southern blot analyses show that the marker is present in a single copy region and is related to auxilin/cyclin-G associated kinase, containing a DnaJ domain. The ELig marker is an important tool that can be used to manage crosses in Eucalyptus breeding programs and inform studies involving lignotuber development and genetics.
publishDate 2020
dc.date.none.fl_str_mv 2020-12-12T01:16:54Z
2020-12-12T01:16:54Z
2020-12-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1038/s41598-020-60308-8
Scientific Reports, v. 10, n. 1, 2020.
2045-2322
http://hdl.handle.net/11449/198588
10.1038/s41598-020-60308-8
2-s2.0-85080972092
0165348738208319
0000-0003-4524-954X
url http://dx.doi.org/10.1038/s41598-020-60308-8
http://hdl.handle.net/11449/198588
identifier_str_mv Scientific Reports, v. 10, n. 1, 2020.
2045-2322
10.1038/s41598-020-60308-8
2-s2.0-85080972092
0165348738208319
0000-0003-4524-954X
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Scientific Reports
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
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