Endoglucanase activity in Neoteredo reynei (Bivalvia, Teredinidae) digestive organs and its content
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1007/s11274-018-2468-x http://hdl.handle.net/11449/171086 |
Resumo: | Cellulolytic enzymes have been studied in several organisms, such as insects, molluscs and other organisms, which can have enzymes endogenously produced or by symbiotic microorganisms. These enzymes are responsible for breaking down the cellulosic material upon which these organisms feed, probably with the aim of assimilating the sugars and nutrients. As Teredinidae bivalves grown in mangrove trees, this study aimed to measure endo-β-1,4-glucanase activity in different organs and its content. Endo-β-1,4-glucanase activity was detected in different organs of the Teredinidae bivalves, including gills and digestive organs tissues and its content. Moreover, organisms such as teredinids grow up inside wood and this process could perhaps be related to creating growth space. All the endoglucanase extracts, from organs tissues and contents, showed maximum activity at 40 °C. The maximum activity was observed at pH 5.5 for all the extracts, except for intestine tissue, which maximum was at pH 6. Moreover, some of the extracts showed a different profile of the activity as a pH influence, suggesting different distribution of enzymes over the digestive system of the teredinids. The results suggested that the endo-β-1,4-glucanase from Teredinidae could be applied in process that requires low temperature, such as, simultaneous saccharification and fermentation, since it presents lower optimum temperature in comparison to enzymes from terrestrial microorganisms. |
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Endoglucanase activity in Neoteredo reynei (Bivalvia, Teredinidae) digestive organs and its contentCellulasesEndo-β-1,4-glucanaseOptimum activityTeredinidaeWood digestionCellulolytic enzymes have been studied in several organisms, such as insects, molluscs and other organisms, which can have enzymes endogenously produced or by symbiotic microorganisms. These enzymes are responsible for breaking down the cellulosic material upon which these organisms feed, probably with the aim of assimilating the sugars and nutrients. As Teredinidae bivalves grown in mangrove trees, this study aimed to measure endo-β-1,4-glucanase activity in different organs and its content. Endo-β-1,4-glucanase activity was detected in different organs of the Teredinidae bivalves, including gills and digestive organs tissues and its content. Moreover, organisms such as teredinids grow up inside wood and this process could perhaps be related to creating growth space. All the endoglucanase extracts, from organs tissues and contents, showed maximum activity at 40 °C. The maximum activity was observed at pH 5.5 for all the extracts, except for intestine tissue, which maximum was at pH 6. Moreover, some of the extracts showed a different profile of the activity as a pH influence, suggesting different distribution of enzymes over the digestive system of the teredinids. The results suggested that the endo-β-1,4-glucanase from Teredinidae could be applied in process that requires low temperature, such as, simultaneous saccharification and fermentation, since it presents lower optimum temperature in comparison to enzymes from terrestrial microorganisms.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ)PetrobrasLaboratory of Biotechnology (Labio) Metrology Applied to Life Science Division - National Institute of Metrology Quality and Technology (Inmetro)Bioenergy Research Institute (IPBEN) Universidade Estadual Paulista (UNESP)Bioenergy Research Institute (IPBEN) Universidade Estadual Paulista (UNESP)CNPq: 550.105/2012-8Petrobras: PFRH-103Quality and Technology (Inmetro)Universidade Estadual Paulista (Unesp)de Moraes Akamine, Daniela Tomade Almeida Cozendey da Silva, Danielde Lima Câmara, GabrielaCarvalho, Thayane VieiraBrienzo, Michel [UNESP]2018-12-11T16:53:50Z2018-12-11T16:53:50Z2018-06-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://dx.doi.org/10.1007/s11274-018-2468-xWorld Journal of Microbiology and Biotechnology, v. 34, n. 6, 2018.1573-09720959-3993http://hdl.handle.net/11449/17108610.1007/s11274-018-2468-x2-s2.0-850481055732-s2.0-85048105573.pdf8251885707409794Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengWorld Journal of Microbiology and Biotechnology0,6040,604info:eu-repo/semantics/openAccess2023-11-08T06:07:43Zoai:repositorio.unesp.br:11449/171086Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T17:08:12.850601Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Endoglucanase activity in Neoteredo reynei (Bivalvia, Teredinidae) digestive organs and its content |
title |
Endoglucanase activity in Neoteredo reynei (Bivalvia, Teredinidae) digestive organs and its content |
spellingShingle |
Endoglucanase activity in Neoteredo reynei (Bivalvia, Teredinidae) digestive organs and its content de Moraes Akamine, Daniela Toma Cellulases Endo-β-1,4-glucanase Optimum activity Teredinidae Wood digestion |
title_short |
Endoglucanase activity in Neoteredo reynei (Bivalvia, Teredinidae) digestive organs and its content |
title_full |
Endoglucanase activity in Neoteredo reynei (Bivalvia, Teredinidae) digestive organs and its content |
title_fullStr |
Endoglucanase activity in Neoteredo reynei (Bivalvia, Teredinidae) digestive organs and its content |
title_full_unstemmed |
Endoglucanase activity in Neoteredo reynei (Bivalvia, Teredinidae) digestive organs and its content |
title_sort |
Endoglucanase activity in Neoteredo reynei (Bivalvia, Teredinidae) digestive organs and its content |
author |
de Moraes Akamine, Daniela Toma |
author_facet |
de Moraes Akamine, Daniela Toma de Almeida Cozendey da Silva, Daniel de Lima Câmara, Gabriela Carvalho, Thayane Vieira Brienzo, Michel [UNESP] |
author_role |
author |
author2 |
de Almeida Cozendey da Silva, Daniel de Lima Câmara, Gabriela Carvalho, Thayane Vieira Brienzo, Michel [UNESP] |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Quality and Technology (Inmetro) Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
de Moraes Akamine, Daniela Toma de Almeida Cozendey da Silva, Daniel de Lima Câmara, Gabriela Carvalho, Thayane Vieira Brienzo, Michel [UNESP] |
dc.subject.por.fl_str_mv |
Cellulases Endo-β-1,4-glucanase Optimum activity Teredinidae Wood digestion |
topic |
Cellulases Endo-β-1,4-glucanase Optimum activity Teredinidae Wood digestion |
description |
Cellulolytic enzymes have been studied in several organisms, such as insects, molluscs and other organisms, which can have enzymes endogenously produced or by symbiotic microorganisms. These enzymes are responsible for breaking down the cellulosic material upon which these organisms feed, probably with the aim of assimilating the sugars and nutrients. As Teredinidae bivalves grown in mangrove trees, this study aimed to measure endo-β-1,4-glucanase activity in different organs and its content. Endo-β-1,4-glucanase activity was detected in different organs of the Teredinidae bivalves, including gills and digestive organs tissues and its content. Moreover, organisms such as teredinids grow up inside wood and this process could perhaps be related to creating growth space. All the endoglucanase extracts, from organs tissues and contents, showed maximum activity at 40 °C. The maximum activity was observed at pH 5.5 for all the extracts, except for intestine tissue, which maximum was at pH 6. Moreover, some of the extracts showed a different profile of the activity as a pH influence, suggesting different distribution of enzymes over the digestive system of the teredinids. The results suggested that the endo-β-1,4-glucanase from Teredinidae could be applied in process that requires low temperature, such as, simultaneous saccharification and fermentation, since it presents lower optimum temperature in comparison to enzymes from terrestrial microorganisms. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-12-11T16:53:50Z 2018-12-11T16:53:50Z 2018-06-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/s11274-018-2468-x World Journal of Microbiology and Biotechnology, v. 34, n. 6, 2018. 1573-0972 0959-3993 http://hdl.handle.net/11449/171086 10.1007/s11274-018-2468-x 2-s2.0-85048105573 2-s2.0-85048105573.pdf 8251885707409794 |
url |
http://dx.doi.org/10.1007/s11274-018-2468-x http://hdl.handle.net/11449/171086 |
identifier_str_mv |
World Journal of Microbiology and Biotechnology, v. 34, n. 6, 2018. 1573-0972 0959-3993 10.1007/s11274-018-2468-x 2-s2.0-85048105573 2-s2.0-85048105573.pdf 8251885707409794 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
World Journal of Microbiology and Biotechnology 0,604 0,604 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1808128759898832896 |