Treatment of partial injury of the calcaneus tendon with heterologous fibrin biopolymer and/or photobiomodulation in rats

Detalhes bibliográficos
Autor(a) principal: de Freitas Dutra Júnior, Enéas
Data de Publicação: 2021
Outros Autores: Hidd, Sílvia Maria Cardoso Magalhães, Amaral, Marcello Magri, Filho, Antonio Luiz Martins Maia, Assis, Livia, Ferreira, Rui Seabra [UNESP], Barraviera, Benedito [UNESP], Martignago, Cintia Cristina Santi, Figueredo-Silva, José, de Oliveira, Rauirys Alencar, Tim, Carla Roberta
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1007/s10103-021-03341-x
http://hdl.handle.net/11449/207773
Resumo: The present study aimed to evaluate the new heterologous fibrin biopolymer associated, or not, with photobiomodulation therapy for application in tendon injuries, considered a serious and common orthopedic problem. Thus, 84 Rattus norvegicus had partial transection of the calcaneus tendon (PTCT) and were randomly divided into: control (CG); heterologous fibrin biopolymer (HFB); photobiomodulation (PBM); heterologous fibrin biopolymer + photobiomodulation (HFB + PBM). The animals received HFB immediately after PTCT, while PBM (660 nm, 40 mW, 0.23 J) started 24 h post injury and followed every 24 h for 7, 14, and 21 days. The results of the edema volume showed that after 24 h of PTCT, there was no statistical difference among the groups. After 7, 14, and 21 days, it was observed that the treatment groups were effective in reducing edema when compared to the control. The HFB had the highest edema volume reduction after 21 days of treatment. The treatment groups did not induce tissue necrosis or infections on the histopathological analysis. Tenocyte proliferation, granulation tissue, and collagen formation were observed in the PTCT area in the HFB and HFB + PBM groups, which culminated a better repair process when compared to the CG in the 3 experimental periods. Interestingly, the PBM group revealed, in histological analysis, major tendon injury after 7 days; however, in the periods of 14 and 21 days, the PBM had a better repair process compared to the CG. In the quantification of collagen, there was no statistical difference between the groups in the 3 experimental periods. The findings suggest that the HFB and PBM treatments, isolated or associated, were effective in reducing the volume of the edema, stimulating the repair process. However, the use of HFB alone was more effective in promoting the tendon repair process. Thus, the present study consolidates previous studies of tendon repair with this new HFB. Future clinical trials will be needed to validate this proposal.
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spelling Treatment of partial injury of the calcaneus tendon with heterologous fibrin biopolymer and/or photobiomodulation in ratsCalcaneus tendonHeterologous fibrin biopolymerPhotobiomodulationRatsTissue repairThe present study aimed to evaluate the new heterologous fibrin biopolymer associated, or not, with photobiomodulation therapy for application in tendon injuries, considered a serious and common orthopedic problem. Thus, 84 Rattus norvegicus had partial transection of the calcaneus tendon (PTCT) and were randomly divided into: control (CG); heterologous fibrin biopolymer (HFB); photobiomodulation (PBM); heterologous fibrin biopolymer + photobiomodulation (HFB + PBM). The animals received HFB immediately after PTCT, while PBM (660 nm, 40 mW, 0.23 J) started 24 h post injury and followed every 24 h for 7, 14, and 21 days. The results of the edema volume showed that after 24 h of PTCT, there was no statistical difference among the groups. After 7, 14, and 21 days, it was observed that the treatment groups were effective in reducing edema when compared to the control. The HFB had the highest edema volume reduction after 21 days of treatment. The treatment groups did not induce tissue necrosis or infections on the histopathological analysis. Tenocyte proliferation, granulation tissue, and collagen formation were observed in the PTCT area in the HFB and HFB + PBM groups, which culminated a better repair process when compared to the CG in the 3 experimental periods. Interestingly, the PBM group revealed, in histological analysis, major tendon injury after 7 days; however, in the periods of 14 and 21 days, the PBM had a better repair process compared to the CG. In the quantification of collagen, there was no statistical difference between the groups in the 3 experimental periods. The findings suggest that the HFB and PBM treatments, isolated or associated, were effective in reducing the volume of the edema, stimulating the repair process. However, the use of HFB alone was more effective in promoting the tendon repair process. Thus, the present study consolidates previous studies of tendon repair with this new HFB. Future clinical trials will be needed to validate this proposal.Department of Biomedical Engineering Instituto Científico E Tecnológico University BrazilBiotechnology and Biodiversity Research Center UESPICenter for the Study of Venoms and Venomous Animals (CEVAP) São Paulo State University (UNESP – Univ Estadual Paulista)Department of Physiotherapy Federal University of São CarlosDepartment of Health Sciences Piauí State University (UESPI)Department of Physiotherapy, Faculdade UninovafapiCenter for the Study of Venoms and Venomous Animals (CEVAP) São Paulo State University (UNESP – Univ Estadual Paulista)University BrazilUESPIUniversidade Estadual Paulista (Unesp)Universidade Federal de São Carlos (UFSCar)Piauí State University (UESPI)de Freitas Dutra Júnior, EnéasHidd, Sílvia Maria Cardoso MagalhãesAmaral, Marcello MagriFilho, Antonio Luiz Martins MaiaAssis, LiviaFerreira, Rui Seabra [UNESP]Barraviera, Benedito [UNESP]Martignago, Cintia Cristina SantiFigueredo-Silva, Joséde Oliveira, Rauirys AlencarTim, Carla Roberta2021-06-25T11:00:45Z2021-06-25T11:00:45Z2021-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1007/s10103-021-03341-xLasers in Medical Science.1435-604X0268-8921http://hdl.handle.net/11449/20777310.1007/s10103-021-03341-x2-s2.0-85106475185Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengLasers in Medical Scienceinfo:eu-repo/semantics/openAccess2024-04-11T15:28:26Zoai:repositorio.unesp.br:11449/207773Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T20:58:02.892794Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Treatment of partial injury of the calcaneus tendon with heterologous fibrin biopolymer and/or photobiomodulation in rats
title Treatment of partial injury of the calcaneus tendon with heterologous fibrin biopolymer and/or photobiomodulation in rats
spellingShingle Treatment of partial injury of the calcaneus tendon with heterologous fibrin biopolymer and/or photobiomodulation in rats
de Freitas Dutra Júnior, Enéas
Calcaneus tendon
Heterologous fibrin biopolymer
Photobiomodulation
Rats
Tissue repair
title_short Treatment of partial injury of the calcaneus tendon with heterologous fibrin biopolymer and/or photobiomodulation in rats
title_full Treatment of partial injury of the calcaneus tendon with heterologous fibrin biopolymer and/or photobiomodulation in rats
title_fullStr Treatment of partial injury of the calcaneus tendon with heterologous fibrin biopolymer and/or photobiomodulation in rats
title_full_unstemmed Treatment of partial injury of the calcaneus tendon with heterologous fibrin biopolymer and/or photobiomodulation in rats
title_sort Treatment of partial injury of the calcaneus tendon with heterologous fibrin biopolymer and/or photobiomodulation in rats
author de Freitas Dutra Júnior, Enéas
author_facet de Freitas Dutra Júnior, Enéas
Hidd, Sílvia Maria Cardoso Magalhães
Amaral, Marcello Magri
Filho, Antonio Luiz Martins Maia
Assis, Livia
Ferreira, Rui Seabra [UNESP]
Barraviera, Benedito [UNESP]
Martignago, Cintia Cristina Santi
Figueredo-Silva, José
de Oliveira, Rauirys Alencar
Tim, Carla Roberta
author_role author
author2 Hidd, Sílvia Maria Cardoso Magalhães
Amaral, Marcello Magri
Filho, Antonio Luiz Martins Maia
Assis, Livia
Ferreira, Rui Seabra [UNESP]
Barraviera, Benedito [UNESP]
Martignago, Cintia Cristina Santi
Figueredo-Silva, José
de Oliveira, Rauirys Alencar
Tim, Carla Roberta
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv University Brazil
UESPI
Universidade Estadual Paulista (Unesp)
Universidade Federal de São Carlos (UFSCar)
Piauí State University (UESPI)
dc.contributor.author.fl_str_mv de Freitas Dutra Júnior, Enéas
Hidd, Sílvia Maria Cardoso Magalhães
Amaral, Marcello Magri
Filho, Antonio Luiz Martins Maia
Assis, Livia
Ferreira, Rui Seabra [UNESP]
Barraviera, Benedito [UNESP]
Martignago, Cintia Cristina Santi
Figueredo-Silva, José
de Oliveira, Rauirys Alencar
Tim, Carla Roberta
dc.subject.por.fl_str_mv Calcaneus tendon
Heterologous fibrin biopolymer
Photobiomodulation
Rats
Tissue repair
topic Calcaneus tendon
Heterologous fibrin biopolymer
Photobiomodulation
Rats
Tissue repair
description The present study aimed to evaluate the new heterologous fibrin biopolymer associated, or not, with photobiomodulation therapy for application in tendon injuries, considered a serious and common orthopedic problem. Thus, 84 Rattus norvegicus had partial transection of the calcaneus tendon (PTCT) and were randomly divided into: control (CG); heterologous fibrin biopolymer (HFB); photobiomodulation (PBM); heterologous fibrin biopolymer + photobiomodulation (HFB + PBM). The animals received HFB immediately after PTCT, while PBM (660 nm, 40 mW, 0.23 J) started 24 h post injury and followed every 24 h for 7, 14, and 21 days. The results of the edema volume showed that after 24 h of PTCT, there was no statistical difference among the groups. After 7, 14, and 21 days, it was observed that the treatment groups were effective in reducing edema when compared to the control. The HFB had the highest edema volume reduction after 21 days of treatment. The treatment groups did not induce tissue necrosis or infections on the histopathological analysis. Tenocyte proliferation, granulation tissue, and collagen formation were observed in the PTCT area in the HFB and HFB + PBM groups, which culminated a better repair process when compared to the CG in the 3 experimental periods. Interestingly, the PBM group revealed, in histological analysis, major tendon injury after 7 days; however, in the periods of 14 and 21 days, the PBM had a better repair process compared to the CG. In the quantification of collagen, there was no statistical difference between the groups in the 3 experimental periods. The findings suggest that the HFB and PBM treatments, isolated or associated, were effective in reducing the volume of the edema, stimulating the repair process. However, the use of HFB alone was more effective in promoting the tendon repair process. Thus, the present study consolidates previous studies of tendon repair with this new HFB. Future clinical trials will be needed to validate this proposal.
publishDate 2021
dc.date.none.fl_str_mv 2021-06-25T11:00:45Z
2021-06-25T11:00:45Z
2021-01-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1007/s10103-021-03341-x
Lasers in Medical Science.
1435-604X
0268-8921
http://hdl.handle.net/11449/207773
10.1007/s10103-021-03341-x
2-s2.0-85106475185
url http://dx.doi.org/10.1007/s10103-021-03341-x
http://hdl.handle.net/11449/207773
identifier_str_mv Lasers in Medical Science.
1435-604X
0268-8921
10.1007/s10103-021-03341-x
2-s2.0-85106475185
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Lasers in Medical Science
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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