Mechanisms of toxic action of copper and copper nanoparticles in two Amazon fish species: Dwarf cichlid (Apistogramma agassizii) and cardinal tetra (Paracheirodon axelrodi)
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.scitotenv.2018.02.216 http://hdl.handle.net/11449/170734 |
Resumo: | Copper oxide nanoparticles (nCuO) are widely used in boat antifouling paints and are released into the environment, potentially inducing toxicity to aquatic organisms. The present study aimed to understand the effects of nCuO and dissolved copper (Cu) on two ornamental Amazon fish species: dwarf cichlid (Apistogramma agassizii) and cardinal tetra (Paracheirodon axelrodi). Fish were exposed to 50% of the LC50 for nCuO (dwarf cichlid 58.31 μg L−1 and cardinal tetra 69.6 μg L−1) and Cu (dwarf cichlid 20 μg L−1 and cardinal tetra 22.9 μg L−1) for 24, 48, 72 and 96 h. Following exposure, aerobic metabolic rate (ṀO2), gill osmoregulatory physiology and mitochondrial function, oxidative stress markers, and morphological damage were evaluated. Our results revealed species specificity in metabolic stress responses. An increase of ṀO2 was noted in cardinal tetra exposed to Cu, but not nCuO, whereas ṀO2 in dwarf cichlid showed little change with either treatment. In contrast, mitochondria from dwarf cichlid exhibited increased proton leak and a resulting decrease in respiratory control ratios in response to nCuO and Cu exposure. This uncoupling was directly related to an increase in reactive oxygen species (ROS) levels. Our findings reveal different metabolic responses between these two species in response to nCuO and Cu, which are probably caused by the differences between species natural histories, indicating that different mechanisms of toxic action of the contaminants are associated to differential osmoregulatory strategies among species. |
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Mechanisms of toxic action of copper and copper nanoparticles in two Amazon fish species: Dwarf cichlid (Apistogramma agassizii) and cardinal tetra (Paracheirodon axelrodi)CuO nanoparticlesMetabolic rateMitochondria respirationOrnamental Amazon fishesOxidative stressReactive oxygen speciesCopper oxide nanoparticles (nCuO) are widely used in boat antifouling paints and are released into the environment, potentially inducing toxicity to aquatic organisms. The present study aimed to understand the effects of nCuO and dissolved copper (Cu) on two ornamental Amazon fish species: dwarf cichlid (Apistogramma agassizii) and cardinal tetra (Paracheirodon axelrodi). Fish were exposed to 50% of the LC50 for nCuO (dwarf cichlid 58.31 μg L−1 and cardinal tetra 69.6 μg L−1) and Cu (dwarf cichlid 20 μg L−1 and cardinal tetra 22.9 μg L−1) for 24, 48, 72 and 96 h. Following exposure, aerobic metabolic rate (ṀO2), gill osmoregulatory physiology and mitochondrial function, oxidative stress markers, and morphological damage were evaluated. Our results revealed species specificity in metabolic stress responses. An increase of ṀO2 was noted in cardinal tetra exposed to Cu, but not nCuO, whereas ṀO2 in dwarf cichlid showed little change with either treatment. In contrast, mitochondria from dwarf cichlid exhibited increased proton leak and a resulting decrease in respiratory control ratios in response to nCuO and Cu exposure. This uncoupling was directly related to an increase in reactive oxygen species (ROS) levels. Our findings reveal different metabolic responses between these two species in response to nCuO and Cu, which are probably caused by the differences between species natural histories, indicating that different mechanisms of toxic action of the contaminants are associated to differential osmoregulatory strategies among species.Fundação de Amparo à Pesquisa do Estado do AmazonasConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Brazilian National Institute for Research of the Amazon Laboratory of Ecophysiology and Molecular Evolution, Ave André Araújo, 2936 AleixoMount Allison University Dept. of Chemistry and Biochemistry, 63C York St.São Paulo State University (UNESP) Institute of BiosciencesSão Paulo State University (UNESP) Institute of BiosciencesFundação de Amparo à Pesquisa do Estado do Amazonas: N° 3159/08CNPq: N° 573976/2008-2Laboratory of Ecophysiology and Molecular EvolutionDept. of Chemistry and BiochemistryUniversidade Estadual Paulista (Unesp)Braz-Mota, SusanaCampos, Derek F.MacCormack, Tyson J.Duarte, Rafael M. [UNESP]Val, Adalberto L.Almeida-Val, Vera M.F.2018-12-11T16:52:13Z2018-12-11T16:52:13Z2018-07-15info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1168-1180application/pdfhttp://dx.doi.org/10.1016/j.scitotenv.2018.02.216Science of the Total Environment, v. 630, p. 1168-1180.1879-10260048-9697http://hdl.handle.net/11449/17073410.1016/j.scitotenv.2018.02.2162-s2.0-850429071032-s2.0-85042907103.pdf30557957777876120000-0001-5649-0692Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengScience of the Total Environment1,546info:eu-repo/semantics/openAccess2023-12-11T06:19:04Zoai:repositorio.unesp.br:11449/170734Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-12-11T06:19:04Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Mechanisms of toxic action of copper and copper nanoparticles in two Amazon fish species: Dwarf cichlid (Apistogramma agassizii) and cardinal tetra (Paracheirodon axelrodi) |
title |
Mechanisms of toxic action of copper and copper nanoparticles in two Amazon fish species: Dwarf cichlid (Apistogramma agassizii) and cardinal tetra (Paracheirodon axelrodi) |
spellingShingle |
Mechanisms of toxic action of copper and copper nanoparticles in two Amazon fish species: Dwarf cichlid (Apistogramma agassizii) and cardinal tetra (Paracheirodon axelrodi) Braz-Mota, Susana CuO nanoparticles Metabolic rate Mitochondria respiration Ornamental Amazon fishes Oxidative stress Reactive oxygen species |
title_short |
Mechanisms of toxic action of copper and copper nanoparticles in two Amazon fish species: Dwarf cichlid (Apistogramma agassizii) and cardinal tetra (Paracheirodon axelrodi) |
title_full |
Mechanisms of toxic action of copper and copper nanoparticles in two Amazon fish species: Dwarf cichlid (Apistogramma agassizii) and cardinal tetra (Paracheirodon axelrodi) |
title_fullStr |
Mechanisms of toxic action of copper and copper nanoparticles in two Amazon fish species: Dwarf cichlid (Apistogramma agassizii) and cardinal tetra (Paracheirodon axelrodi) |
title_full_unstemmed |
Mechanisms of toxic action of copper and copper nanoparticles in two Amazon fish species: Dwarf cichlid (Apistogramma agassizii) and cardinal tetra (Paracheirodon axelrodi) |
title_sort |
Mechanisms of toxic action of copper and copper nanoparticles in two Amazon fish species: Dwarf cichlid (Apistogramma agassizii) and cardinal tetra (Paracheirodon axelrodi) |
author |
Braz-Mota, Susana |
author_facet |
Braz-Mota, Susana Campos, Derek F. MacCormack, Tyson J. Duarte, Rafael M. [UNESP] Val, Adalberto L. Almeida-Val, Vera M.F. |
author_role |
author |
author2 |
Campos, Derek F. MacCormack, Tyson J. Duarte, Rafael M. [UNESP] Val, Adalberto L. Almeida-Val, Vera M.F. |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Laboratory of Ecophysiology and Molecular Evolution Dept. of Chemistry and Biochemistry Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Braz-Mota, Susana Campos, Derek F. MacCormack, Tyson J. Duarte, Rafael M. [UNESP] Val, Adalberto L. Almeida-Val, Vera M.F. |
dc.subject.por.fl_str_mv |
CuO nanoparticles Metabolic rate Mitochondria respiration Ornamental Amazon fishes Oxidative stress Reactive oxygen species |
topic |
CuO nanoparticles Metabolic rate Mitochondria respiration Ornamental Amazon fishes Oxidative stress Reactive oxygen species |
description |
Copper oxide nanoparticles (nCuO) are widely used in boat antifouling paints and are released into the environment, potentially inducing toxicity to aquatic organisms. The present study aimed to understand the effects of nCuO and dissolved copper (Cu) on two ornamental Amazon fish species: dwarf cichlid (Apistogramma agassizii) and cardinal tetra (Paracheirodon axelrodi). Fish were exposed to 50% of the LC50 for nCuO (dwarf cichlid 58.31 μg L−1 and cardinal tetra 69.6 μg L−1) and Cu (dwarf cichlid 20 μg L−1 and cardinal tetra 22.9 μg L−1) for 24, 48, 72 and 96 h. Following exposure, aerobic metabolic rate (ṀO2), gill osmoregulatory physiology and mitochondrial function, oxidative stress markers, and morphological damage were evaluated. Our results revealed species specificity in metabolic stress responses. An increase of ṀO2 was noted in cardinal tetra exposed to Cu, but not nCuO, whereas ṀO2 in dwarf cichlid showed little change with either treatment. In contrast, mitochondria from dwarf cichlid exhibited increased proton leak and a resulting decrease in respiratory control ratios in response to nCuO and Cu exposure. This uncoupling was directly related to an increase in reactive oxygen species (ROS) levels. Our findings reveal different metabolic responses between these two species in response to nCuO and Cu, which are probably caused by the differences between species natural histories, indicating that different mechanisms of toxic action of the contaminants are associated to differential osmoregulatory strategies among species. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-12-11T16:52:13Z 2018-12-11T16:52:13Z 2018-07-15 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.scitotenv.2018.02.216 Science of the Total Environment, v. 630, p. 1168-1180. 1879-1026 0048-9697 http://hdl.handle.net/11449/170734 10.1016/j.scitotenv.2018.02.216 2-s2.0-85042907103 2-s2.0-85042907103.pdf 3055795777787612 0000-0001-5649-0692 |
url |
http://dx.doi.org/10.1016/j.scitotenv.2018.02.216 http://hdl.handle.net/11449/170734 |
identifier_str_mv |
Science of the Total Environment, v. 630, p. 1168-1180. 1879-1026 0048-9697 10.1016/j.scitotenv.2018.02.216 2-s2.0-85042907103 2-s2.0-85042907103.pdf 3055795777787612 0000-0001-5649-0692 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Science of the Total Environment 1,546 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1168-1180 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1799965252974018560 |