An eco-friendly and low-cost method for the quantification of Cefazolin sodium in powder for injectable solution using thin-layer chromatography assisted by digital images
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1556/1006.2017.30.4.8 http://hdl.handle.net/11449/179029 |
Resumo: | Cefazolin sodium (CFZ) is a semi-synthetic first-generation cephalosporin used for the treatment of infections caused by Gram-positive and Gram-negative microorganisms. Although it is a drug used worldwide, there are only a few green analytical methods described in the literature for its quantification. This work proposes an eco-friendly, low-cost, and rapid analytical method to analyze CFZ in lyophilized powder for injectable solution based on digital images, using thin-layer chromatography (TLC). For the TLC method, the mobile phase was comprised of ethanol and water, and the spots revelation was performed using iodine vapors. The TLC spots were analyzed through digital images, obtained by flatbed scanner, and evaluated using the free software The Gimp (GNU Image Manipulation Program), by quantification of pixels in pictures. This method was validated according to the International Conference on Harmonization (ICH) guidelines and presented adequate linearity, accuracy, precision, and robustness. It also showed its selectivity and stability-indicating capability (under acid, alkaline, aqueous, oxidative, and photolytic stress conditions). This is an innovative, easy, and low-cost method that can be used for the analysis of cefazolin sodium in lyophilized powder. Considering that this method does not use toxic organic solvents for the environment and operators, it contributes to the development of green chemistry. |
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An eco-friendly and low-cost method for the quantification of Cefazolin sodium in powder for injectable solution using thin-layer chromatography assisted by digital imagesAnalytical methodsCefazolin sodiumDigital images in quality controlGreen chemistryStabilityThin-layer chromatographyCefazolin sodium (CFZ) is a semi-synthetic first-generation cephalosporin used for the treatment of infections caused by Gram-positive and Gram-negative microorganisms. Although it is a drug used worldwide, there are only a few green analytical methods described in the literature for its quantification. This work proposes an eco-friendly, low-cost, and rapid analytical method to analyze CFZ in lyophilized powder for injectable solution based on digital images, using thin-layer chromatography (TLC). For the TLC method, the mobile phase was comprised of ethanol and water, and the spots revelation was performed using iodine vapors. The TLC spots were analyzed through digital images, obtained by flatbed scanner, and evaluated using the free software The Gimp (GNU Image Manipulation Program), by quantification of pixels in pictures. This method was validated according to the International Conference on Harmonization (ICH) guidelines and presented adequate linearity, accuracy, precision, and robustness. It also showed its selectivity and stability-indicating capability (under acid, alkaline, aqueous, oxidative, and photolytic stress conditions). This is an innovative, easy, and low-cost method that can be used for the analysis of cefazolin sodium in lyophilized powder. Considering that this method does not use toxic organic solvents for the environment and operators, it contributes to the development of green chemistry.Institute of Biochemistry and Biotechnology Faculty of Natural Science I Martin-Luther-University Halle-WittenbergDepartment of Pharmaceutics School of Pharmaceutical Sciences of Araraquara Universidade Estadual Paulista - UNESPDepartment of Pharmaceutics School of Pharmaceutical Sciences of Araraquara Universidade Estadual Paulista - UNESPMartin-Luther-University Halle-WittenbergUniversidade Estadual Paulista (Unesp)Zimmermann, AlexanderTótoli, Eliane Gandolpho [UNESP]Fernandes, Felipe Hugo Alencar [UNESP]Salgado, Hérida Regina Nunes [UNESP]2018-12-11T17:33:14Z2018-12-11T17:33:14Z2017-08-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article285-290http://dx.doi.org/10.1556/1006.2017.30.4.8Journal of Planar Chromatography - Modern TLC, v. 30, n. 4, p. 285-290, 2017.1789-09930933-4173http://hdl.handle.net/11449/17902910.1556/1006.2017.30.4.82-s2.0-85023757213Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Planar Chromatography - Modern TLC0,249info:eu-repo/semantics/openAccess2024-06-24T13:45:18Zoai:repositorio.unesp.br:11449/179029Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T15:10:41.072603Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
An eco-friendly and low-cost method for the quantification of Cefazolin sodium in powder for injectable solution using thin-layer chromatography assisted by digital images |
title |
An eco-friendly and low-cost method for the quantification of Cefazolin sodium in powder for injectable solution using thin-layer chromatography assisted by digital images |
spellingShingle |
An eco-friendly and low-cost method for the quantification of Cefazolin sodium in powder for injectable solution using thin-layer chromatography assisted by digital images Zimmermann, Alexander Analytical methods Cefazolin sodium Digital images in quality control Green chemistry Stability Thin-layer chromatography |
title_short |
An eco-friendly and low-cost method for the quantification of Cefazolin sodium in powder for injectable solution using thin-layer chromatography assisted by digital images |
title_full |
An eco-friendly and low-cost method for the quantification of Cefazolin sodium in powder for injectable solution using thin-layer chromatography assisted by digital images |
title_fullStr |
An eco-friendly and low-cost method for the quantification of Cefazolin sodium in powder for injectable solution using thin-layer chromatography assisted by digital images |
title_full_unstemmed |
An eco-friendly and low-cost method for the quantification of Cefazolin sodium in powder for injectable solution using thin-layer chromatography assisted by digital images |
title_sort |
An eco-friendly and low-cost method for the quantification of Cefazolin sodium in powder for injectable solution using thin-layer chromatography assisted by digital images |
author |
Zimmermann, Alexander |
author_facet |
Zimmermann, Alexander Tótoli, Eliane Gandolpho [UNESP] Fernandes, Felipe Hugo Alencar [UNESP] Salgado, Hérida Regina Nunes [UNESP] |
author_role |
author |
author2 |
Tótoli, Eliane Gandolpho [UNESP] Fernandes, Felipe Hugo Alencar [UNESP] Salgado, Hérida Regina Nunes [UNESP] |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Martin-Luther-University Halle-Wittenberg Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Zimmermann, Alexander Tótoli, Eliane Gandolpho [UNESP] Fernandes, Felipe Hugo Alencar [UNESP] Salgado, Hérida Regina Nunes [UNESP] |
dc.subject.por.fl_str_mv |
Analytical methods Cefazolin sodium Digital images in quality control Green chemistry Stability Thin-layer chromatography |
topic |
Analytical methods Cefazolin sodium Digital images in quality control Green chemistry Stability Thin-layer chromatography |
description |
Cefazolin sodium (CFZ) is a semi-synthetic first-generation cephalosporin used for the treatment of infections caused by Gram-positive and Gram-negative microorganisms. Although it is a drug used worldwide, there are only a few green analytical methods described in the literature for its quantification. This work proposes an eco-friendly, low-cost, and rapid analytical method to analyze CFZ in lyophilized powder for injectable solution based on digital images, using thin-layer chromatography (TLC). For the TLC method, the mobile phase was comprised of ethanol and water, and the spots revelation was performed using iodine vapors. The TLC spots were analyzed through digital images, obtained by flatbed scanner, and evaluated using the free software The Gimp (GNU Image Manipulation Program), by quantification of pixels in pictures. This method was validated according to the International Conference on Harmonization (ICH) guidelines and presented adequate linearity, accuracy, precision, and robustness. It also showed its selectivity and stability-indicating capability (under acid, alkaline, aqueous, oxidative, and photolytic stress conditions). This is an innovative, easy, and low-cost method that can be used for the analysis of cefazolin sodium in lyophilized powder. Considering that this method does not use toxic organic solvents for the environment and operators, it contributes to the development of green chemistry. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-08-01 2018-12-11T17:33:14Z 2018-12-11T17:33:14Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1556/1006.2017.30.4.8 Journal of Planar Chromatography - Modern TLC, v. 30, n. 4, p. 285-290, 2017. 1789-0993 0933-4173 http://hdl.handle.net/11449/179029 10.1556/1006.2017.30.4.8 2-s2.0-85023757213 |
url |
http://dx.doi.org/10.1556/1006.2017.30.4.8 http://hdl.handle.net/11449/179029 |
identifier_str_mv |
Journal of Planar Chromatography - Modern TLC, v. 30, n. 4, p. 285-290, 2017. 1789-0993 0933-4173 10.1556/1006.2017.30.4.8 2-s2.0-85023757213 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Planar Chromatography - Modern TLC 0,249 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
285-290 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128475573256192 |