Pulmonary disease in hamsters infected with Leptospira interrogans: Histopathologic findings and cytokine mRNA expressions
Autor(a) principal: | |
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Data de Publicação: | 2009 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.4269/ajtmh.2009.80.832 http://hdl.handle.net/11449/225482 |
Resumo: | Our aim was to evaluate the pulmonary changes induced by Leptospira interrogans infection in hamsters, and the gene expression of endogenous mediators in lung fragments during 28 days of observation. The animals were euthanized on days 4, 7, 14, 21, and 28 post-inoculation. Histopathologic lung analysis showed hemorrhage, pneumonia, alveolar congestion, and infiltrated cellular areas, with increasing severity until day 21 post-inoculation. Tumor necrosis factor (TNF)-α mRNA expression enhanced in first days with peak on day 4 and slightly decreased in the final phase. The interleukin (IL)-10 remained relatively constant throughout the period, with the exceptions of days 4 and 14. The endothelial nitric-oxide synthesis (eNOS) showed an increased expression on day 4, followed by an augment on days 7 and 14, and remaining constant up to day 28 post-infection. Our results demonstrate that inoculation of L. interrogans sorovar Icterohaemorrhagiae induced pulmonary lesions, including pulmonary hemorrhage, supporting that the lung is a target organ. Copyright © 2009 by The American Society of Tropical Medicine and Hygiene. |
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Pulmonary disease in hamsters infected with Leptospira interrogans: Histopathologic findings and cytokine mRNA expressionsOur aim was to evaluate the pulmonary changes induced by Leptospira interrogans infection in hamsters, and the gene expression of endogenous mediators in lung fragments during 28 days of observation. The animals were euthanized on days 4, 7, 14, 21, and 28 post-inoculation. Histopathologic lung analysis showed hemorrhage, pneumonia, alveolar congestion, and infiltrated cellular areas, with increasing severity until day 21 post-inoculation. Tumor necrosis factor (TNF)-α mRNA expression enhanced in first days with peak on day 4 and slightly decreased in the final phase. The interleukin (IL)-10 remained relatively constant throughout the period, with the exceptions of days 4 and 14. The endothelial nitric-oxide synthesis (eNOS) showed an increased expression on day 4, followed by an augment on days 7 and 14, and remaining constant up to day 28 post-infection. Our results demonstrate that inoculation of L. interrogans sorovar Icterohaemorrhagiae induced pulmonary lesions, including pulmonary hemorrhage, supporting that the lung is a target organ. Copyright © 2009 by The American Society of Tropical Medicine and Hygiene.Department of Animal Health and Production Microbiology Laboratory FOA Unesp-Estadual Paulista University, Araçatuba, São PauloInflammatory Mediators Laboratory Geriatrics and Gerontology Division Federal University of São PauloInfectious Diseases Division Federal University of São PauloUniversidade Estadual Paulista-Unesp-FOA-Medicina Veterinária- Campus de Araçatuba, Rua Clóvis Pestana, 793, CEP: 16050-680, Araçatuba, São PauloMicrobiology Laboratory Estadual Paulista University Unesp-FOA-Campus Aracatuba, Rua Clóvis Pestana, 793, CEP: 16050-680, Araçatuba, São PauloInflammatory Mediators Laboratory Geriatrics and Gerontology Division Federal University, São PauloDepartment of Animal Health and Production Estadual Paulista University Unesp-FOA-Campus de Araçatuba, São PauloDepartment of Animal Health and Production Estadual Paulista University Unesp-FOA-Campus Araçatuba, São PauloInfectious Diseases Division Federal University, São PauloDepartment of Animal Health and Production Microbiology Laboratory FOA Unesp-Estadual Paulista University, Araçatuba, São PauloUniversidade Estadual Paulista-Unesp-FOA-Medicina Veterinária- Campus de Araçatuba, Rua Clóvis Pestana, 793, CEP: 16050-680, Araçatuba, São PauloMicrobiology Laboratory Estadual Paulista University Unesp-FOA-Campus Aracatuba, Rua Clóvis Pestana, 793, CEP: 16050-680, Araçatuba, São PauloDepartment of Animal Health and Production Estadual Paulista University Unesp-FOA-Campus de Araçatuba, São PauloDepartment of Animal Health and Production Estadual Paulista University Unesp-FOA-Campus Araçatuba, São PauloUniversidade Estadual Paulista (UNESP)Universidade de São Paulo (USP)Federal UniversityMarinho, Márcia [UNESP]Oliveira-Júnior, Itamar Souza [UNESP]Monteiro, Cristina Maria Rodrigues [UNESP]Perri, Silvia Helena [UNESP]Salomão, Reinaldo [UNESP]2022-04-28T20:52:15Z2022-04-28T20:52:15Z2009-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article832-836http://dx.doi.org/10.4269/ajtmh.2009.80.832American Journal of Tropical Medicine and Hygiene, v. 80, n. 5, p. 832-836, 2009.0002-9637http://hdl.handle.net/11449/22548210.4269/ajtmh.2009.80.8322-s2.0-66149131059Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengAmerican Journal of Tropical Medicine and Hygieneinfo:eu-repo/semantics/openAccess2022-04-28T20:52:15Zoai:repositorio.unesp.br:11449/225482Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462022-04-28T20:52:15Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Pulmonary disease in hamsters infected with Leptospira interrogans: Histopathologic findings and cytokine mRNA expressions |
title |
Pulmonary disease in hamsters infected with Leptospira interrogans: Histopathologic findings and cytokine mRNA expressions |
spellingShingle |
Pulmonary disease in hamsters infected with Leptospira interrogans: Histopathologic findings and cytokine mRNA expressions Marinho, Márcia [UNESP] |
title_short |
Pulmonary disease in hamsters infected with Leptospira interrogans: Histopathologic findings and cytokine mRNA expressions |
title_full |
Pulmonary disease in hamsters infected with Leptospira interrogans: Histopathologic findings and cytokine mRNA expressions |
title_fullStr |
Pulmonary disease in hamsters infected with Leptospira interrogans: Histopathologic findings and cytokine mRNA expressions |
title_full_unstemmed |
Pulmonary disease in hamsters infected with Leptospira interrogans: Histopathologic findings and cytokine mRNA expressions |
title_sort |
Pulmonary disease in hamsters infected with Leptospira interrogans: Histopathologic findings and cytokine mRNA expressions |
author |
Marinho, Márcia [UNESP] |
author_facet |
Marinho, Márcia [UNESP] Oliveira-Júnior, Itamar Souza [UNESP] Monteiro, Cristina Maria Rodrigues [UNESP] Perri, Silvia Helena [UNESP] Salomão, Reinaldo [UNESP] |
author_role |
author |
author2 |
Oliveira-Júnior, Itamar Souza [UNESP] Monteiro, Cristina Maria Rodrigues [UNESP] Perri, Silvia Helena [UNESP] Salomão, Reinaldo [UNESP] |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) Universidade de São Paulo (USP) Federal University |
dc.contributor.author.fl_str_mv |
Marinho, Márcia [UNESP] Oliveira-Júnior, Itamar Souza [UNESP] Monteiro, Cristina Maria Rodrigues [UNESP] Perri, Silvia Helena [UNESP] Salomão, Reinaldo [UNESP] |
description |
Our aim was to evaluate the pulmonary changes induced by Leptospira interrogans infection in hamsters, and the gene expression of endogenous mediators in lung fragments during 28 days of observation. The animals were euthanized on days 4, 7, 14, 21, and 28 post-inoculation. Histopathologic lung analysis showed hemorrhage, pneumonia, alveolar congestion, and infiltrated cellular areas, with increasing severity until day 21 post-inoculation. Tumor necrosis factor (TNF)-α mRNA expression enhanced in first days with peak on day 4 and slightly decreased in the final phase. The interleukin (IL)-10 remained relatively constant throughout the period, with the exceptions of days 4 and 14. The endothelial nitric-oxide synthesis (eNOS) showed an increased expression on day 4, followed by an augment on days 7 and 14, and remaining constant up to day 28 post-infection. Our results demonstrate that inoculation of L. interrogans sorovar Icterohaemorrhagiae induced pulmonary lesions, including pulmonary hemorrhage, supporting that the lung is a target organ. Copyright © 2009 by The American Society of Tropical Medicine and Hygiene. |
publishDate |
2009 |
dc.date.none.fl_str_mv |
2009-01-01 2022-04-28T20:52:15Z 2022-04-28T20:52:15Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.4269/ajtmh.2009.80.832 American Journal of Tropical Medicine and Hygiene, v. 80, n. 5, p. 832-836, 2009. 0002-9637 http://hdl.handle.net/11449/225482 10.4269/ajtmh.2009.80.832 2-s2.0-66149131059 |
url |
http://dx.doi.org/10.4269/ajtmh.2009.80.832 http://hdl.handle.net/11449/225482 |
identifier_str_mv |
American Journal of Tropical Medicine and Hygiene, v. 80, n. 5, p. 832-836, 2009. 0002-9637 10.4269/ajtmh.2009.80.832 2-s2.0-66149131059 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
American Journal of Tropical Medicine and Hygiene |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
832-836 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799964484027023360 |