Nanoporous silk films with capillary action and size-exclusion capacity for sensitive glucose determination in whole blood
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1039/d0lc00702a http://hdl.handle.net/11449/205874 |
Resumo: | In optical biosensing, silk fibroin (SF) appears as a promising alternative where other materials, such as paper, find limitations. Besides its excellent optical properties and unmet capacity to stabilize biomacromolecules, SF in test strips exhibits additional functions,i.e.capillary pumping activity of 1.5 mm s−1, capacity to filter blood cells thanks to its small, but tuneable, porosity and enhanced biosensing sensitivity. The bulk functionalization of SF with the enzymes glucose oxidase and peroxidase and the mediator ABTS produces colourless and transparent SF films that respond to blood glucose increasing 2.5 times the sensitivity of conventional ABTS-based assays. This enhanced sensitivity results from the formation of SF-ABTS complexes, where SF becomes part of the bioassay. Additionally, SF films triple the durability of most stable cellulose-based sensors. Although demonstrated for glucose, SF microfluidic test strips may incorporate other optical bioassays,e.g.immunoassays, with the aim of transferring them from central laboratories to the place of patient's care. |
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Repositório Institucional da UNESP |
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spelling |
Nanoporous silk films with capillary action and size-exclusion capacity for sensitive glucose determination in whole bloodIn optical biosensing, silk fibroin (SF) appears as a promising alternative where other materials, such as paper, find limitations. Besides its excellent optical properties and unmet capacity to stabilize biomacromolecules, SF in test strips exhibits additional functions,i.e.capillary pumping activity of 1.5 mm s−1, capacity to filter blood cells thanks to its small, but tuneable, porosity and enhanced biosensing sensitivity. The bulk functionalization of SF with the enzymes glucose oxidase and peroxidase and the mediator ABTS produces colourless and transparent SF films that respond to blood glucose increasing 2.5 times the sensitivity of conventional ABTS-based assays. This enhanced sensitivity results from the formation of SF-ABTS complexes, where SF becomes part of the bioassay. Additionally, SF films triple the durability of most stable cellulose-based sensors. Although demonstrated for glucose, SF microfluidic test strips may incorporate other optical bioassays,e.g.immunoassays, with the aim of transferring them from central laboratories to the place of patient's care.Instituto de Microelectrónica de Barcelona (IMB-CNM CSIC)São Carlos Institute of Physics University of São PauloDepartament de Química Universitat Autònoma de Barcelona Bellaterra (Barcelona)Instituto Murciano de Investigación y Desarrollo Agrario y Alimentario (IMIDA)Institute of Chemistry São Paulo State University (UNESP)Silklab Department of Biomedical Engineering Tufts UniversityDepartment of Electrical and Computer Engineering Tufts UniversityDepartment of Physics Tufts UniversityLaboratory for Living Devices Tufts UniversityInstitute of Chemistry São Paulo State University (UNESP)CSIC)Universidade de São Paulo (USP)Universitat Autònoma de Barcelona Bellaterra (Barcelona)Instituto Murciano de Investigación y Desarrollo Agrario y Alimentario (IMIDA)Universidade Estadual Paulista (Unesp)Tufts UniversityMárquez, AugustoSantos, Moliria V.Guirado, GonzaloMoreno, AlexAznar-Cervantes, Salvador D.Cenis, Jose LuisSantagneli, Silvia H. [UNESP]Domínguez, CarlosOmenetto, Fiorenzo G.Muñoz-Berbel, Xavier2021-06-25T10:22:39Z2021-06-25T10:22:39Z2021-02-07info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article608-615http://dx.doi.org/10.1039/d0lc00702aLab on a Chip, v. 21, n. 3, p. 608-615, 2021.1473-01891473-0197http://hdl.handle.net/11449/20587410.1039/d0lc00702a2-s2.0-85100812402Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengLab on a Chipinfo:eu-repo/semantics/openAccess2021-10-22T19:32:45Zoai:repositorio.unesp.br:11449/205874Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T18:41:37.668620Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Nanoporous silk films with capillary action and size-exclusion capacity for sensitive glucose determination in whole blood |
title |
Nanoporous silk films with capillary action and size-exclusion capacity for sensitive glucose determination in whole blood |
spellingShingle |
Nanoporous silk films with capillary action and size-exclusion capacity for sensitive glucose determination in whole blood Márquez, Augusto |
title_short |
Nanoporous silk films with capillary action and size-exclusion capacity for sensitive glucose determination in whole blood |
title_full |
Nanoporous silk films with capillary action and size-exclusion capacity for sensitive glucose determination in whole blood |
title_fullStr |
Nanoporous silk films with capillary action and size-exclusion capacity for sensitive glucose determination in whole blood |
title_full_unstemmed |
Nanoporous silk films with capillary action and size-exclusion capacity for sensitive glucose determination in whole blood |
title_sort |
Nanoporous silk films with capillary action and size-exclusion capacity for sensitive glucose determination in whole blood |
author |
Márquez, Augusto |
author_facet |
Márquez, Augusto Santos, Moliria V. Guirado, Gonzalo Moreno, Alex Aznar-Cervantes, Salvador D. Cenis, Jose Luis Santagneli, Silvia H. [UNESP] Domínguez, Carlos Omenetto, Fiorenzo G. Muñoz-Berbel, Xavier |
author_role |
author |
author2 |
Santos, Moliria V. Guirado, Gonzalo Moreno, Alex Aznar-Cervantes, Salvador D. Cenis, Jose Luis Santagneli, Silvia H. [UNESP] Domínguez, Carlos Omenetto, Fiorenzo G. Muñoz-Berbel, Xavier |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
CSIC) Universidade de São Paulo (USP) Universitat Autònoma de Barcelona Bellaterra (Barcelona) Instituto Murciano de Investigación y Desarrollo Agrario y Alimentario (IMIDA) Universidade Estadual Paulista (Unesp) Tufts University |
dc.contributor.author.fl_str_mv |
Márquez, Augusto Santos, Moliria V. Guirado, Gonzalo Moreno, Alex Aznar-Cervantes, Salvador D. Cenis, Jose Luis Santagneli, Silvia H. [UNESP] Domínguez, Carlos Omenetto, Fiorenzo G. Muñoz-Berbel, Xavier |
description |
In optical biosensing, silk fibroin (SF) appears as a promising alternative where other materials, such as paper, find limitations. Besides its excellent optical properties and unmet capacity to stabilize biomacromolecules, SF in test strips exhibits additional functions,i.e.capillary pumping activity of 1.5 mm s−1, capacity to filter blood cells thanks to its small, but tuneable, porosity and enhanced biosensing sensitivity. The bulk functionalization of SF with the enzymes glucose oxidase and peroxidase and the mediator ABTS produces colourless and transparent SF films that respond to blood glucose increasing 2.5 times the sensitivity of conventional ABTS-based assays. This enhanced sensitivity results from the formation of SF-ABTS complexes, where SF becomes part of the bioassay. Additionally, SF films triple the durability of most stable cellulose-based sensors. Although demonstrated for glucose, SF microfluidic test strips may incorporate other optical bioassays,e.g.immunoassays, with the aim of transferring them from central laboratories to the place of patient's care. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-06-25T10:22:39Z 2021-06-25T10:22:39Z 2021-02-07 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1039/d0lc00702a Lab on a Chip, v. 21, n. 3, p. 608-615, 2021. 1473-0189 1473-0197 http://hdl.handle.net/11449/205874 10.1039/d0lc00702a 2-s2.0-85100812402 |
url |
http://dx.doi.org/10.1039/d0lc00702a http://hdl.handle.net/11449/205874 |
identifier_str_mv |
Lab on a Chip, v. 21, n. 3, p. 608-615, 2021. 1473-0189 1473-0197 10.1039/d0lc00702a 2-s2.0-85100812402 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Lab on a Chip |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
608-615 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128967073333248 |