Nanoporous silk films with capillary action and size-exclusion capacity for sensitive glucose determination in whole blood

Detalhes bibliográficos
Autor(a) principal: Márquez, Augusto
Data de Publicação: 2021
Outros Autores: Santos, Moliria V., Guirado, Gonzalo, Moreno, Alex, Aznar-Cervantes, Salvador D., Cenis, Jose Luis, Santagneli, Silvia H. [UNESP], Domínguez, Carlos, Omenetto, Fiorenzo G., Muñoz-Berbel, Xavier
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1039/d0lc00702a
http://hdl.handle.net/11449/205874
Resumo: In optical biosensing, silk fibroin (SF) appears as a promising alternative where other materials, such as paper, find limitations. Besides its excellent optical properties and unmet capacity to stabilize biomacromolecules, SF in test strips exhibits additional functions,i.e.capillary pumping activity of 1.5 mm s−1, capacity to filter blood cells thanks to its small, but tuneable, porosity and enhanced biosensing sensitivity. The bulk functionalization of SF with the enzymes glucose oxidase and peroxidase and the mediator ABTS produces colourless and transparent SF films that respond to blood glucose increasing 2.5 times the sensitivity of conventional ABTS-based assays. This enhanced sensitivity results from the formation of SF-ABTS complexes, where SF becomes part of the bioassay. Additionally, SF films triple the durability of most stable cellulose-based sensors. Although demonstrated for glucose, SF microfluidic test strips may incorporate other optical bioassays,e.g.immunoassays, with the aim of transferring them from central laboratories to the place of patient's care.
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spelling Nanoporous silk films with capillary action and size-exclusion capacity for sensitive glucose determination in whole bloodIn optical biosensing, silk fibroin (SF) appears as a promising alternative where other materials, such as paper, find limitations. Besides its excellent optical properties and unmet capacity to stabilize biomacromolecules, SF in test strips exhibits additional functions,i.e.capillary pumping activity of 1.5 mm s−1, capacity to filter blood cells thanks to its small, but tuneable, porosity and enhanced biosensing sensitivity. The bulk functionalization of SF with the enzymes glucose oxidase and peroxidase and the mediator ABTS produces colourless and transparent SF films that respond to blood glucose increasing 2.5 times the sensitivity of conventional ABTS-based assays. This enhanced sensitivity results from the formation of SF-ABTS complexes, where SF becomes part of the bioassay. Additionally, SF films triple the durability of most stable cellulose-based sensors. Although demonstrated for glucose, SF microfluidic test strips may incorporate other optical bioassays,e.g.immunoassays, with the aim of transferring them from central laboratories to the place of patient's care.Instituto de Microelectrónica de Barcelona (IMB-CNM CSIC)São Carlos Institute of Physics University of São PauloDepartament de Química Universitat Autònoma de Barcelona Bellaterra (Barcelona)Instituto Murciano de Investigación y Desarrollo Agrario y Alimentario (IMIDA)Institute of Chemistry São Paulo State University (UNESP)Silklab Department of Biomedical Engineering Tufts UniversityDepartment of Electrical and Computer Engineering Tufts UniversityDepartment of Physics Tufts UniversityLaboratory for Living Devices Tufts UniversityInstitute of Chemistry São Paulo State University (UNESP)CSIC)Universidade de São Paulo (USP)Universitat Autònoma de Barcelona Bellaterra (Barcelona)Instituto Murciano de Investigación y Desarrollo Agrario y Alimentario (IMIDA)Universidade Estadual Paulista (Unesp)Tufts UniversityMárquez, AugustoSantos, Moliria V.Guirado, GonzaloMoreno, AlexAznar-Cervantes, Salvador D.Cenis, Jose LuisSantagneli, Silvia H. [UNESP]Domínguez, CarlosOmenetto, Fiorenzo G.Muñoz-Berbel, Xavier2021-06-25T10:22:39Z2021-06-25T10:22:39Z2021-02-07info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article608-615http://dx.doi.org/10.1039/d0lc00702aLab on a Chip, v. 21, n. 3, p. 608-615, 2021.1473-01891473-0197http://hdl.handle.net/11449/20587410.1039/d0lc00702a2-s2.0-85100812402Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengLab on a Chipinfo:eu-repo/semantics/openAccess2021-10-22T19:32:45Zoai:repositorio.unesp.br:11449/205874Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T18:41:37.668620Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Nanoporous silk films with capillary action and size-exclusion capacity for sensitive glucose determination in whole blood
title Nanoporous silk films with capillary action and size-exclusion capacity for sensitive glucose determination in whole blood
spellingShingle Nanoporous silk films with capillary action and size-exclusion capacity for sensitive glucose determination in whole blood
Márquez, Augusto
title_short Nanoporous silk films with capillary action and size-exclusion capacity for sensitive glucose determination in whole blood
title_full Nanoporous silk films with capillary action and size-exclusion capacity for sensitive glucose determination in whole blood
title_fullStr Nanoporous silk films with capillary action and size-exclusion capacity for sensitive glucose determination in whole blood
title_full_unstemmed Nanoporous silk films with capillary action and size-exclusion capacity for sensitive glucose determination in whole blood
title_sort Nanoporous silk films with capillary action and size-exclusion capacity for sensitive glucose determination in whole blood
author Márquez, Augusto
author_facet Márquez, Augusto
Santos, Moliria V.
Guirado, Gonzalo
Moreno, Alex
Aznar-Cervantes, Salvador D.
Cenis, Jose Luis
Santagneli, Silvia H. [UNESP]
Domínguez, Carlos
Omenetto, Fiorenzo G.
Muñoz-Berbel, Xavier
author_role author
author2 Santos, Moliria V.
Guirado, Gonzalo
Moreno, Alex
Aznar-Cervantes, Salvador D.
Cenis, Jose Luis
Santagneli, Silvia H. [UNESP]
Domínguez, Carlos
Omenetto, Fiorenzo G.
Muñoz-Berbel, Xavier
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv CSIC)
Universidade de São Paulo (USP)
Universitat Autònoma de Barcelona Bellaterra (Barcelona)
Instituto Murciano de Investigación y Desarrollo Agrario y Alimentario (IMIDA)
Universidade Estadual Paulista (Unesp)
Tufts University
dc.contributor.author.fl_str_mv Márquez, Augusto
Santos, Moliria V.
Guirado, Gonzalo
Moreno, Alex
Aznar-Cervantes, Salvador D.
Cenis, Jose Luis
Santagneli, Silvia H. [UNESP]
Domínguez, Carlos
Omenetto, Fiorenzo G.
Muñoz-Berbel, Xavier
description In optical biosensing, silk fibroin (SF) appears as a promising alternative where other materials, such as paper, find limitations. Besides its excellent optical properties and unmet capacity to stabilize biomacromolecules, SF in test strips exhibits additional functions,i.e.capillary pumping activity of 1.5 mm s−1, capacity to filter blood cells thanks to its small, but tuneable, porosity and enhanced biosensing sensitivity. The bulk functionalization of SF with the enzymes glucose oxidase and peroxidase and the mediator ABTS produces colourless and transparent SF films that respond to blood glucose increasing 2.5 times the sensitivity of conventional ABTS-based assays. This enhanced sensitivity results from the formation of SF-ABTS complexes, where SF becomes part of the bioassay. Additionally, SF films triple the durability of most stable cellulose-based sensors. Although demonstrated for glucose, SF microfluidic test strips may incorporate other optical bioassays,e.g.immunoassays, with the aim of transferring them from central laboratories to the place of patient's care.
publishDate 2021
dc.date.none.fl_str_mv 2021-06-25T10:22:39Z
2021-06-25T10:22:39Z
2021-02-07
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1039/d0lc00702a
Lab on a Chip, v. 21, n. 3, p. 608-615, 2021.
1473-0189
1473-0197
http://hdl.handle.net/11449/205874
10.1039/d0lc00702a
2-s2.0-85100812402
url http://dx.doi.org/10.1039/d0lc00702a
http://hdl.handle.net/11449/205874
identifier_str_mv Lab on a Chip, v. 21, n. 3, p. 608-615, 2021.
1473-0189
1473-0197
10.1039/d0lc00702a
2-s2.0-85100812402
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Lab on a Chip
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 608-615
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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