Explant culture: A relevant tool for the study of telocytes

Detalhes bibliográficos
Autor(a) principal: Sanches, Bruno D. A.
Data de Publicação: 2020
Outros Autores: Maldarine, Juliana D. S., Tamarindo, Guilherme H., Da Silva, Alana D. T. [UNESP], Lima, Maria L. D. [UNESP], Rahal, Paula [UNESP], Góes, Rejane M. [UNESP], Taboga, Sebastião R. [UNESP], Carvalho, Hernandes F.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1002/cbin.11446
http://hdl.handle.net/11449/200970
Resumo: Telocytes are cells present in the stroma of various tissues including the prostate. The detection of telocytes is still very much dependent on obtaining ultrastructural data that show the presence of telopodes, which are cytoplasmic projections that alternate between dilated regions, the podoms, and thin segments, the podomers. These structures are the distinctive characteristics of the telocytes. Thus, in vitro assays are important for the study of telocytes, which are more easily identified in culture, which also enables the experimental manipulation of these cells. The isolation of telocytes per se does not allow the analysis of the behavior of these cells in relation to other cell types in a given organ. In this sense, in the prostate, explants could be a useful tool for the study of telocytes. The present study obtained prostatic explants and evaluated the influence of recombinant proteins, scattering factor (SCF) and stromal-derived factor 1 (SDF-1), which could impact on the migration of CD34-positive cells. Telocytes migrate out of explants and SDF-1 stimulates the proliferation and formation of telocyte networks in vitro. Telocytes are not smooth muscle cell progenitors in the prostate; on the contrary, they are CD90- and CD44-negative cells and, hence, have limited progenitor capacity. The present study demonstrated that explants are useful tools to elucidate the nature of telocytes and their functions.
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spelling Explant culture: A relevant tool for the study of telocytesc-KitCD34prostateSCFSDF-1smooth muscle cellsTelocytes are cells present in the stroma of various tissues including the prostate. The detection of telocytes is still very much dependent on obtaining ultrastructural data that show the presence of telopodes, which are cytoplasmic projections that alternate between dilated regions, the podoms, and thin segments, the podomers. These structures are the distinctive characteristics of the telocytes. Thus, in vitro assays are important for the study of telocytes, which are more easily identified in culture, which also enables the experimental manipulation of these cells. The isolation of telocytes per se does not allow the analysis of the behavior of these cells in relation to other cell types in a given organ. In this sense, in the prostate, explants could be a useful tool for the study of telocytes. The present study obtained prostatic explants and evaluated the influence of recombinant proteins, scattering factor (SCF) and stromal-derived factor 1 (SDF-1), which could impact on the migration of CD34-positive cells. Telocytes migrate out of explants and SDF-1 stimulates the proliferation and formation of telocyte networks in vitro. Telocytes are not smooth muscle cell progenitors in the prostate; on the contrary, they are CD90- and CD44-negative cells and, hence, have limited progenitor capacity. The present study demonstrated that explants are useful tools to elucidate the nature of telocytes and their functions.Department of Structural and Functional Biology Institute of Biology State University of Campinas (UNICAMP)Department of Biology Laboratory of Microscopy and Microanalysis São Paulo State University (UNESP)Department of Biology Laboratory of Genome Studies São Paulo State University (UNESP)Department of Biology Laboratory of Microscopy and Microanalysis São Paulo State University (UNESP)Department of Biology Laboratory of Genome Studies São Paulo State University (UNESP)Universidade Estadual de Campinas (UNICAMP)Universidade Estadual Paulista (Unesp)Sanches, Bruno D. A.Maldarine, Juliana D. S.Tamarindo, Guilherme H.Da Silva, Alana D. T. [UNESP]Lima, Maria L. D. [UNESP]Rahal, Paula [UNESP]Góes, Rejane M. [UNESP]Taboga, Sebastião R. [UNESP]Carvalho, Hernandes F.2020-12-12T02:20:51Z2020-12-12T02:20:51Z2020-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1002/cbin.11446Cell Biology International.1095-83551065-6995http://hdl.handle.net/11449/20097010.1002/cbin.114462-s2.0-8508999272879910823626712120000-0001-5693-6148Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengCell Biology Internationalinfo:eu-repo/semantics/openAccess2024-04-11T19:33:50Zoai:repositorio.unesp.br:11449/200970Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T15:30:31.075481Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Explant culture: A relevant tool for the study of telocytes
title Explant culture: A relevant tool for the study of telocytes
spellingShingle Explant culture: A relevant tool for the study of telocytes
Sanches, Bruno D. A.
c-Kit
CD34
prostate
SCF
SDF-1
smooth muscle cells
title_short Explant culture: A relevant tool for the study of telocytes
title_full Explant culture: A relevant tool for the study of telocytes
title_fullStr Explant culture: A relevant tool for the study of telocytes
title_full_unstemmed Explant culture: A relevant tool for the study of telocytes
title_sort Explant culture: A relevant tool for the study of telocytes
author Sanches, Bruno D. A.
author_facet Sanches, Bruno D. A.
Maldarine, Juliana D. S.
Tamarindo, Guilherme H.
Da Silva, Alana D. T. [UNESP]
Lima, Maria L. D. [UNESP]
Rahal, Paula [UNESP]
Góes, Rejane M. [UNESP]
Taboga, Sebastião R. [UNESP]
Carvalho, Hernandes F.
author_role author
author2 Maldarine, Juliana D. S.
Tamarindo, Guilherme H.
Da Silva, Alana D. T. [UNESP]
Lima, Maria L. D. [UNESP]
Rahal, Paula [UNESP]
Góes, Rejane M. [UNESP]
Taboga, Sebastião R. [UNESP]
Carvalho, Hernandes F.
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual de Campinas (UNICAMP)
Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Sanches, Bruno D. A.
Maldarine, Juliana D. S.
Tamarindo, Guilherme H.
Da Silva, Alana D. T. [UNESP]
Lima, Maria L. D. [UNESP]
Rahal, Paula [UNESP]
Góes, Rejane M. [UNESP]
Taboga, Sebastião R. [UNESP]
Carvalho, Hernandes F.
dc.subject.por.fl_str_mv c-Kit
CD34
prostate
SCF
SDF-1
smooth muscle cells
topic c-Kit
CD34
prostate
SCF
SDF-1
smooth muscle cells
description Telocytes are cells present in the stroma of various tissues including the prostate. The detection of telocytes is still very much dependent on obtaining ultrastructural data that show the presence of telopodes, which are cytoplasmic projections that alternate between dilated regions, the podoms, and thin segments, the podomers. These structures are the distinctive characteristics of the telocytes. Thus, in vitro assays are important for the study of telocytes, which are more easily identified in culture, which also enables the experimental manipulation of these cells. The isolation of telocytes per se does not allow the analysis of the behavior of these cells in relation to other cell types in a given organ. In this sense, in the prostate, explants could be a useful tool for the study of telocytes. The present study obtained prostatic explants and evaluated the influence of recombinant proteins, scattering factor (SCF) and stromal-derived factor 1 (SDF-1), which could impact on the migration of CD34-positive cells. Telocytes migrate out of explants and SDF-1 stimulates the proliferation and formation of telocyte networks in vitro. Telocytes are not smooth muscle cell progenitors in the prostate; on the contrary, they are CD90- and CD44-negative cells and, hence, have limited progenitor capacity. The present study demonstrated that explants are useful tools to elucidate the nature of telocytes and their functions.
publishDate 2020
dc.date.none.fl_str_mv 2020-12-12T02:20:51Z
2020-12-12T02:20:51Z
2020-01-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1002/cbin.11446
Cell Biology International.
1095-8355
1065-6995
http://hdl.handle.net/11449/200970
10.1002/cbin.11446
2-s2.0-85089992728
7991082362671212
0000-0001-5693-6148
url http://dx.doi.org/10.1002/cbin.11446
http://hdl.handle.net/11449/200970
identifier_str_mv Cell Biology International.
1095-8355
1065-6995
10.1002/cbin.11446
2-s2.0-85089992728
7991082362671212
0000-0001-5693-6148
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Cell Biology International
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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