Inventário da ictiofauna do alto rio Paraíba do Sul e caracterização molecular das espécies encontradas

• Autor(a) principal: Maia, Gláucia Maria Garcia [UNESP]
• Data de Publicação: 2012
• Tipo de documento: Trabalho de conclusão de curso
• Idioma: por
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://hdl.handle.net/11449/119766
• topic: Peixe - Identificação; Ictiofauna - Inventarios; Ichthyofauna; Paraiba do Sul, Rio, Bacia

The state of São Paulo has four main drainages: Paraná river, Paraíba do Sul river, Ribeira do Iguape river and coastal rivers. The Paraíba do Sul river is born in Sao Paulo and drains an important range of land east of the state. Its ichthyofauna has some similarities and many differences from the continental and coastal drainages which highlights the importance of this study. Surveys conducted in the ichthyofauna of this basin, as in other large river basins in Brazil, is still incomplete. Moreover, there is no consensus about the taxonomic status of many species listed in these surveys. Considering the promising use of DNA barcode as a global system for species identification, the present study is aimed to establishing an inventory of the ichthyofauna of the São Paulo portion of the river Paraíba do Sul and simultaneously build a DNA barcode reference sequence library for fish found. Were obtained and analyzed 354 sequences of the gene cytochrome oxidase c subunit I (COI) belonging to 66 species of São Paulo portion of the Paraíba do Sul river. The average K2P distance between individuals within species of this basin was 0.48%, and 9,87% between species within a genus. Five pairs of species (10 species) showed low levels of interspecific genetic divergence (<2%),but all could be correctly identified. This study showed that the fish species analyzed could be identified efficiently through the use of barcode generating data that can provide information for further studies of this fauna, besides contributing to the global initiative to characterize the species of fish in the world of a molecular point of view. Five pairs of species (10 species) showed low levels of interspecific genetic divergence (<2%), but all could be correctly identified. This study showed that the fish species analyzed could be identified efficiently through the use of barcode generating data that can provide subsidies for further studies in this fauna, as well as ...