Purificação da enzima bromelina presente no curauá (Ananas erectifolius L.B. Smith) variedade roxa, por sistema bifásico aquoso PEG 4000/fosfato de potássio

• Autor(a) principal: Ferreira, Juliana Ferrari
• Data de Publicação: 2011
• Outros Autores: Sbruzzi, Dalva, Barros, Kleber Vânio Gomes, Machado, Isaac Stringueta [UNESP], Tambourgi, Elias Basile
• Tipo de documento: Artigo
• Idioma: por
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://www.deag.ufcg.edu.br/rbpa/rev132.html; http://hdl.handle.net/11449/140397
• topic: Ananas erectifolius; Bromelain; Purification; Aqueous two-phase systems; pH; Ananas erectifolius; Bromelina; Purificação; Sistema bifásico aquoso; pH

Curauá (Ananas erectifolius LB Smith) is a typical plant of northern and central-western Brazil. It produces a highly strong fiber with applications in several areas, mainly in the automotive industry. Belonging to the family of Bromeliaceae, it contains significant levels of bromelain, a group of proteolysis’ enzymes with application in several areas such as in the food, pharmaceutical and cosmetics industries. In this work we evaluated the purification of the enzyme present in the leaves of Curauá by means of an aqueous two-phase system PEG 4000/Potassium phosphate. The enzyme had its activity measured in each phase by the Biuret method. The purification factor (PF) was obtained in order to optimize the conditions for purification of the bromelain. It was used 03 pH 7.0, 8.0 and 9.0 values, varying the proportional composition in between the polymeric and the saline phases (tie-lines). It was used the purple variety of Curauá. The results showed that PEG system 4000/ potassium phosphate polymer with a higher concentration and at pH 7.0 produced better results in enzyme purification.