Ceratoplastia lamelar em coelhos usando enxerto de omento al�geno livre e c�lulas mesenquimais omentais associadas com membrana amni�tica canina

• Autor(a) principal: De Barros, S�fora Vieira Da Silva Gouv�a [UNESP]
• Data de Publicação: 2016
• Outros Autores: Aldrovani, Marcela [UNESP], De Lacerda, Luciana Cen�o Correa [UNESP], Horr, M�nica [UNESP], Marinho, F�bio Andrade [UNESP], Lima, Tiago Barbalho [UNESP], Da Silveira, Camila Pinho Balthazar [UNESP], Brito, F�bio Luiz Da Cunha [UNESP], Bassaneze, Vin�cius, Flecher, Mayra Cunha, Beltrame, Renato Travassos, Nakamuta, Juliana Sanajotti, Krieger, Jos� Eduardo, Laus, Jos� Luiz [UNESP]
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://dx.doi.org/10.1590/0103-8478cr20151348; http://hdl.handle.net/11449/173400
• topic: Amniotic membrane; Cornea; Omentum; Progenitor cells; Wound healing

The objective of this research was to evaluate the clinical and microscopic effects in rabbits of lamellar keratoplasty using allogeneic omentum associated with canine amniotic membrane (AM). Rabbits were divided into two groups: one received the allogeneic free omental graft covered with the AM (OM-graft group), while the other received the AM graft containing omental mesenchymal cells (OM-cell group). Clinical signs were evaluated on different postoperative days. After the clinical assessments, the rabbits were euthanized and their corneas were obtained for histopathology and immunohistochemistry (Ki-67, marker for proliferation). Both groups showed chemosis, blepharospasm, eye discharge, hyperemia, and corneal opacity/ edema. Neovascularization was observed in the OM-cell group. Histopathological evaluation revealed epithelial islands within the stroma of OM-cell samples. Thirty days after surgery, complete corneal re-epithelialization had occurred in both groups. The OM cell group showed more Ki-67 positive cells. The free omentum and its cells, combined with the AM, contributed to corneal repair, a process that was completed 30 days after lamellar keratoplasty.