Ceratoplastia lamelar em coelhos usando enxerto de omento al�geno livre e c�lulas mesenquimais omentais associadas com membrana amni�tica canina

Detalhes bibliográficos
Autor(a) principal: De Barros, S�fora Vieira Da Silva Gouv�a [UNESP]
Data de Publicação: 2016
Outros Autores: Aldrovani, Marcela [UNESP], De Lacerda, Luciana Cen�o Correa [UNESP], Horr, M�nica [UNESP], Marinho, F�bio Andrade [UNESP], Lima, Tiago Barbalho [UNESP], Da Silveira, Camila Pinho Balthazar [UNESP], Brito, F�bio Luiz Da Cunha [UNESP], Bassaneze, Vin�cius, Flecher, Mayra Cunha, Beltrame, Renato Travassos, Nakamuta, Juliana Sanajotti, Krieger, Jos� Eduardo, Laus, Jos� Luiz [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1590/0103-8478cr20151348
http://hdl.handle.net/11449/173400
Resumo: The objective of this research was to evaluate the clinical and microscopic effects in rabbits of lamellar keratoplasty using allogeneic omentum associated with canine amniotic membrane (AM). Rabbits were divided into two groups: one received the allogeneic free omental graft covered with the AM (OM-graft group), while the other received the AM graft containing omental mesenchymal cells (OM-cell group). Clinical signs were evaluated on different postoperative days. After the clinical assessments, the rabbits were euthanized and their corneas were obtained for histopathology and immunohistochemistry (Ki-67, marker for proliferation). Both groups showed chemosis, blepharospasm, eye discharge, hyperemia, and corneal opacity/ edema. Neovascularization was observed in the OM-cell group. Histopathological evaluation revealed epithelial islands within the stroma of OM-cell samples. Thirty days after surgery, complete corneal re-epithelialization had occurred in both groups. The OM cell group showed more Ki-67 positive cells. The free omentum and its cells, combined with the AM, contributed to corneal repair, a process that was completed 30 days after lamellar keratoplasty.
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spelling Ceratoplastia lamelar em coelhos usando enxerto de omento al�geno livre e c�lulas mesenquimais omentais associadas com membrana amni�tica caninaLamellar keratoplasty in rabbits using an allogeneic free omental graft and omentum-derived mesenchymal cells associated with the canine amniotic membraneAmniotic membraneCorneaOmentumProgenitor cellsWound healingThe objective of this research was to evaluate the clinical and microscopic effects in rabbits of lamellar keratoplasty using allogeneic omentum associated with canine amniotic membrane (AM). Rabbits were divided into two groups: one received the allogeneic free omental graft covered with the AM (OM-graft group), while the other received the AM graft containing omental mesenchymal cells (OM-cell group). Clinical signs were evaluated on different postoperative days. After the clinical assessments, the rabbits were euthanized and their corneas were obtained for histopathology and immunohistochemistry (Ki-67, marker for proliferation). Both groups showed chemosis, blepharospasm, eye discharge, hyperemia, and corneal opacity/ edema. Neovascularization was observed in the OM-cell group. Histopathological evaluation revealed epithelial islands within the stroma of OM-cell samples. Thirty days after surgery, complete corneal re-epithelialization had occurred in both groups. The OM cell group showed more Ki-67 positive cells. The free omentum and its cells, combined with the AM, contributed to corneal repair, a process that was completed 30 days after lamellar keratoplasty.Departamento de Cl�nica e Cirurgia Faculdade de Ci�ncias Agr�rias e Veterin�rias (FCAV) Universidade Estadual Paulista (UNESP), Campus de JaboticabalInstituto do Cora��o (InCor) Escola de Medicina Universidade de S�o Paulo (USP)Departamento de Cl�nica e Cirurgia Faculdade de Ci�ncias Agr�rias e Veterin�rias (FCAV) Universidade Estadual Paulista (UNESP), Campus de JaboticabalUniversidade Estadual Paulista (Unesp)Universidade de São Paulo (USP)De Barros, S�fora Vieira Da Silva Gouv�a [UNESP]Aldrovani, Marcela [UNESP]De Lacerda, Luciana Cen�o Correa [UNESP]Horr, M�nica [UNESP]Marinho, F�bio Andrade [UNESP]Lima, Tiago Barbalho [UNESP]Da Silveira, Camila Pinho Balthazar [UNESP]Brito, F�bio Luiz Da Cunha [UNESP]Bassaneze, Vin�ciusFlecher, Mayra CunhaBeltrame, Renato TravassosNakamuta, Juliana SanajottiKrieger, Jos� EduardoLaus, Jos� Luiz [UNESP]2018-12-11T17:05:01Z2018-12-11T17:05:01Z2016-10-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1838-1845application/pdfhttp://dx.doi.org/10.1590/0103-8478cr20151348Ciencia Rural, v. 46, n. 10, p. 1838-1845, 2016.1678-45960103-8478http://hdl.handle.net/11449/17340010.1590/0103-8478cr20151348S0103-847820160010018382-s2.0-84983504237S0103-84782016001001838.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengCiencia Rural0,337info:eu-repo/semantics/openAccess2024-06-06T14:10:09Zoai:repositorio.unesp.br:11449/173400Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T19:08:40.354769Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Ceratoplastia lamelar em coelhos usando enxerto de omento al�geno livre e c�lulas mesenquimais omentais associadas com membrana amni�tica canina
Lamellar keratoplasty in rabbits using an allogeneic free omental graft and omentum-derived mesenchymal cells associated with the canine amniotic membrane
title Ceratoplastia lamelar em coelhos usando enxerto de omento al�geno livre e c�lulas mesenquimais omentais associadas com membrana amni�tica canina
spellingShingle Ceratoplastia lamelar em coelhos usando enxerto de omento al�geno livre e c�lulas mesenquimais omentais associadas com membrana amni�tica canina
De Barros, S�fora Vieira Da Silva Gouv�a [UNESP]
Amniotic membrane
Cornea
Omentum
Progenitor cells
Wound healing
title_short Ceratoplastia lamelar em coelhos usando enxerto de omento al�geno livre e c�lulas mesenquimais omentais associadas com membrana amni�tica canina
title_full Ceratoplastia lamelar em coelhos usando enxerto de omento al�geno livre e c�lulas mesenquimais omentais associadas com membrana amni�tica canina
title_fullStr Ceratoplastia lamelar em coelhos usando enxerto de omento al�geno livre e c�lulas mesenquimais omentais associadas com membrana amni�tica canina
title_full_unstemmed Ceratoplastia lamelar em coelhos usando enxerto de omento al�geno livre e c�lulas mesenquimais omentais associadas com membrana amni�tica canina
title_sort Ceratoplastia lamelar em coelhos usando enxerto de omento al�geno livre e c�lulas mesenquimais omentais associadas com membrana amni�tica canina
author De Barros, S�fora Vieira Da Silva Gouv�a [UNESP]
author_facet De Barros, S�fora Vieira Da Silva Gouv�a [UNESP]
Aldrovani, Marcela [UNESP]
De Lacerda, Luciana Cen�o Correa [UNESP]
Horr, M�nica [UNESP]
Marinho, F�bio Andrade [UNESP]
Lima, Tiago Barbalho [UNESP]
Da Silveira, Camila Pinho Balthazar [UNESP]
Brito, F�bio Luiz Da Cunha [UNESP]
Bassaneze, Vin�cius
Flecher, Mayra Cunha
Beltrame, Renato Travassos
Nakamuta, Juliana Sanajotti
Krieger, Jos� Eduardo
Laus, Jos� Luiz [UNESP]
author_role author
author2 Aldrovani, Marcela [UNESP]
De Lacerda, Luciana Cen�o Correa [UNESP]
Horr, M�nica [UNESP]
Marinho, F�bio Andrade [UNESP]
Lima, Tiago Barbalho [UNESP]
Da Silveira, Camila Pinho Balthazar [UNESP]
Brito, F�bio Luiz Da Cunha [UNESP]
Bassaneze, Vin�cius
Flecher, Mayra Cunha
Beltrame, Renato Travassos
Nakamuta, Juliana Sanajotti
Krieger, Jos� Eduardo
Laus, Jos� Luiz [UNESP]
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Universidade de São Paulo (USP)
dc.contributor.author.fl_str_mv De Barros, S�fora Vieira Da Silva Gouv�a [UNESP]
Aldrovani, Marcela [UNESP]
De Lacerda, Luciana Cen�o Correa [UNESP]
Horr, M�nica [UNESP]
Marinho, F�bio Andrade [UNESP]
Lima, Tiago Barbalho [UNESP]
Da Silveira, Camila Pinho Balthazar [UNESP]
Brito, F�bio Luiz Da Cunha [UNESP]
Bassaneze, Vin�cius
Flecher, Mayra Cunha
Beltrame, Renato Travassos
Nakamuta, Juliana Sanajotti
Krieger, Jos� Eduardo
Laus, Jos� Luiz [UNESP]
dc.subject.por.fl_str_mv Amniotic membrane
Cornea
Omentum
Progenitor cells
Wound healing
topic Amniotic membrane
Cornea
Omentum
Progenitor cells
Wound healing
description The objective of this research was to evaluate the clinical and microscopic effects in rabbits of lamellar keratoplasty using allogeneic omentum associated with canine amniotic membrane (AM). Rabbits were divided into two groups: one received the allogeneic free omental graft covered with the AM (OM-graft group), while the other received the AM graft containing omental mesenchymal cells (OM-cell group). Clinical signs were evaluated on different postoperative days. After the clinical assessments, the rabbits were euthanized and their corneas were obtained for histopathology and immunohistochemistry (Ki-67, marker for proliferation). Both groups showed chemosis, blepharospasm, eye discharge, hyperemia, and corneal opacity/ edema. Neovascularization was observed in the OM-cell group. Histopathological evaluation revealed epithelial islands within the stroma of OM-cell samples. Thirty days after surgery, complete corneal re-epithelialization had occurred in both groups. The OM cell group showed more Ki-67 positive cells. The free omentum and its cells, combined with the AM, contributed to corneal repair, a process that was completed 30 days after lamellar keratoplasty.
publishDate 2016
dc.date.none.fl_str_mv 2016-10-01
2018-12-11T17:05:01Z
2018-12-11T17:05:01Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/0103-8478cr20151348
Ciencia Rural, v. 46, n. 10, p. 1838-1845, 2016.
1678-4596
0103-8478
http://hdl.handle.net/11449/173400
10.1590/0103-8478cr20151348
S0103-84782016001001838
2-s2.0-84983504237
S0103-84782016001001838.pdf
url http://dx.doi.org/10.1590/0103-8478cr20151348
http://hdl.handle.net/11449/173400
identifier_str_mv Ciencia Rural, v. 46, n. 10, p. 1838-1845, 2016.
1678-4596
0103-8478
10.1590/0103-8478cr20151348
S0103-84782016001001838
2-s2.0-84983504237
S0103-84782016001001838.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Ciencia Rural
0,337
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 1838-1845
application/pdf
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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