Bactericidal Effect of Entomopathogenic Bacterium Pseudomonas entomophila Against Xanthomonas citri Reduces Citrus Canker Disease Severity

Detalhes bibliográficos
Autor(a) principal: Villamizar, Sonia [UNESP]
Data de Publicação: 2020
Outros Autores: Ferro, Jesus Aparecido [UNESP], Caicedo, Juan Carlos [UNESP], Alves, L. M.C. [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.3389/fmicb.2020.01431
http://hdl.handle.net/11449/201942
Resumo: The bacterium Pseudomonas entomophila has been recognized as an exceptional species within the Pseudomonas genus, capable of naturally infecting and killing insects from at least three different orders. P. entomophila ingestion leads to irreversible gut damage resulting from a global blockage of translation, which impairs both immune and tissue repair systems in the insect intestine. In this study we isolated a P. entomophila bacterial strain from soil samples which displayed a strong activity against Xanthomonas citri subsp, citri (Xcc), the etiological agent of citrus canker disease. The antagonism potential of isolated bacteria against Xcc and its ability to reduce citrus canker severity was assessed both ex planta and in planta. Our findings show that pathogenicity assays in Citrus x limonia by pressure infiltration and spray with a mixture of P. entomophila and Xcc leaded to a significant reduction in the number of canker lesions in high susceptible citrus leaves, at 21 days post-infection. To the best of our knowledge this is the first report of antibacterial activity of P. entomophila against a phytopathogenic bacterium. Collective action of P. entomophila factors such as diketopiperazine production and the type 6 secretion system (T6SS) may be involved in this type of biological control of citrus canker. The results suggest that the P. entomophila strain could be a promising biocontrol agent acting directly against Xcc.
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spelling Bactericidal Effect of Entomopathogenic Bacterium Pseudomonas entomophila Against Xanthomonas citri Reduces Citrus Canker Disease Severityantagonism abilityanti bacterialbiological controlCitrus limoniaNRPSsecondary metabolitesThe bacterium Pseudomonas entomophila has been recognized as an exceptional species within the Pseudomonas genus, capable of naturally infecting and killing insects from at least three different orders. P. entomophila ingestion leads to irreversible gut damage resulting from a global blockage of translation, which impairs both immune and tissue repair systems in the insect intestine. In this study we isolated a P. entomophila bacterial strain from soil samples which displayed a strong activity against Xanthomonas citri subsp, citri (Xcc), the etiological agent of citrus canker disease. The antagonism potential of isolated bacteria against Xcc and its ability to reduce citrus canker severity was assessed both ex planta and in planta. Our findings show that pathogenicity assays in Citrus x limonia by pressure infiltration and spray with a mixture of P. entomophila and Xcc leaded to a significant reduction in the number of canker lesions in high susceptible citrus leaves, at 21 days post-infection. To the best of our knowledge this is the first report of antibacterial activity of P. entomophila against a phytopathogenic bacterium. Collective action of P. entomophila factors such as diketopiperazine production and the type 6 secretion system (T6SS) may be involved in this type of biological control of citrus canker. The results suggest that the P. entomophila strain could be a promising biocontrol agent acting directly against Xcc.Post Graduate Program in Agricultural and Livestock Microbiology School of Agricultural and Veterinary Sciences São Paulo State University (UNESP)Faculty of Exact Natural and Agricultural Sciences Research Group CIBAS Universidad de Santander (UDES)Post Graduate Program in Agricultural and Livestock Microbiology School of Agricultural and Veterinary Sciences São Paulo State University (UNESP)Universidade Estadual Paulista (Unesp)Universidad de Santander (UDES)Villamizar, Sonia [UNESP]Ferro, Jesus Aparecido [UNESP]Caicedo, Juan Carlos [UNESP]Alves, L. M.C. [UNESP]2020-12-12T02:45:49Z2020-12-12T02:45:49Z2020-06-24info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.3389/fmicb.2020.01431Frontiers in Microbiology, v. 11.1664-302Xhttp://hdl.handle.net/11449/20194210.3389/fmicb.2020.014312-s2.0-85087682335Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengFrontiers in Microbiologyinfo:eu-repo/semantics/openAccess2024-06-07T15:32:00Zoai:repositorio.unesp.br:11449/201942Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T19:01:33.448086Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Bactericidal Effect of Entomopathogenic Bacterium Pseudomonas entomophila Against Xanthomonas citri Reduces Citrus Canker Disease Severity
title Bactericidal Effect of Entomopathogenic Bacterium Pseudomonas entomophila Against Xanthomonas citri Reduces Citrus Canker Disease Severity
spellingShingle Bactericidal Effect of Entomopathogenic Bacterium Pseudomonas entomophila Against Xanthomonas citri Reduces Citrus Canker Disease Severity
Villamizar, Sonia [UNESP]
antagonism ability
anti bacterial
biological control
Citrus limonia
NRPS
secondary metabolites
title_short Bactericidal Effect of Entomopathogenic Bacterium Pseudomonas entomophila Against Xanthomonas citri Reduces Citrus Canker Disease Severity
title_full Bactericidal Effect of Entomopathogenic Bacterium Pseudomonas entomophila Against Xanthomonas citri Reduces Citrus Canker Disease Severity
title_fullStr Bactericidal Effect of Entomopathogenic Bacterium Pseudomonas entomophila Against Xanthomonas citri Reduces Citrus Canker Disease Severity
title_full_unstemmed Bactericidal Effect of Entomopathogenic Bacterium Pseudomonas entomophila Against Xanthomonas citri Reduces Citrus Canker Disease Severity
title_sort Bactericidal Effect of Entomopathogenic Bacterium Pseudomonas entomophila Against Xanthomonas citri Reduces Citrus Canker Disease Severity
author Villamizar, Sonia [UNESP]
author_facet Villamizar, Sonia [UNESP]
Ferro, Jesus Aparecido [UNESP]
Caicedo, Juan Carlos [UNESP]
Alves, L. M.C. [UNESP]
author_role author
author2 Ferro, Jesus Aparecido [UNESP]
Caicedo, Juan Carlos [UNESP]
Alves, L. M.C. [UNESP]
author2_role author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Universidad de Santander (UDES)
dc.contributor.author.fl_str_mv Villamizar, Sonia [UNESP]
Ferro, Jesus Aparecido [UNESP]
Caicedo, Juan Carlos [UNESP]
Alves, L. M.C. [UNESP]
dc.subject.por.fl_str_mv antagonism ability
anti bacterial
biological control
Citrus limonia
NRPS
secondary metabolites
topic antagonism ability
anti bacterial
biological control
Citrus limonia
NRPS
secondary metabolites
description The bacterium Pseudomonas entomophila has been recognized as an exceptional species within the Pseudomonas genus, capable of naturally infecting and killing insects from at least three different orders. P. entomophila ingestion leads to irreversible gut damage resulting from a global blockage of translation, which impairs both immune and tissue repair systems in the insect intestine. In this study we isolated a P. entomophila bacterial strain from soil samples which displayed a strong activity against Xanthomonas citri subsp, citri (Xcc), the etiological agent of citrus canker disease. The antagonism potential of isolated bacteria against Xcc and its ability to reduce citrus canker severity was assessed both ex planta and in planta. Our findings show that pathogenicity assays in Citrus x limonia by pressure infiltration and spray with a mixture of P. entomophila and Xcc leaded to a significant reduction in the number of canker lesions in high susceptible citrus leaves, at 21 days post-infection. To the best of our knowledge this is the first report of antibacterial activity of P. entomophila against a phytopathogenic bacterium. Collective action of P. entomophila factors such as diketopiperazine production and the type 6 secretion system (T6SS) may be involved in this type of biological control of citrus canker. The results suggest that the P. entomophila strain could be a promising biocontrol agent acting directly against Xcc.
publishDate 2020
dc.date.none.fl_str_mv 2020-12-12T02:45:49Z
2020-12-12T02:45:49Z
2020-06-24
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.3389/fmicb.2020.01431
Frontiers in Microbiology, v. 11.
1664-302X
http://hdl.handle.net/11449/201942
10.3389/fmicb.2020.01431
2-s2.0-85087682335
url http://dx.doi.org/10.3389/fmicb.2020.01431
http://hdl.handle.net/11449/201942
identifier_str_mv Frontiers in Microbiology, v. 11.
1664-302X
10.3389/fmicb.2020.01431
2-s2.0-85087682335
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Frontiers in Microbiology
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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