Pre-hatching embryo-dependent and -independent programming of endometrial function in cattle
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1371/journal.pone.0175954 http://hdl.handle.net/11449/162738 |
Resumo: | The bovine pre-implantation embryo secretes bioactive molecules from early development stages, but effects on endometrial function are reported to start only after elongation. Here, we interrogated spatially defined regions of the endometrium transcriptome for responses to a day 7 embryo in vivo. We hypothesize that exposure to an embryo changes the abundance of specific transcripts in the cranial region of the pregnant uterine horn. Endometrium was collected from the uterotubal junction (UTJ), anterior (IA), medial (IM) and posterior (IP) regions of the uterine horn ipsilateral to the CL 7 days after estrus from sham-inseminated (Con) or artificially inseminated, confirmed pregnant (Preg) cows. Abundance of 86 transcripts was evaluated by qPCR using a microfluidic platform. Abundance of 12 transcripts was modulated in the Preg endometrium, including classical interferon-stimulated genes (ISG15, MX1, MX2 and OAS1Y), prostaglandin biosynthesis genes (PTGES, HPGD and AKR1C4), water channel (AQP4) and a solute transporter (SLC1A4) and this was in the UTJ and IA mainly. Additionally, for 71 transcripts, abundance varied according to region of the reproductive tract. Regulation included downregulation of genes associated with proliferation (IGF1, IGF2, IGF1R and IGF2R) and extracellular matrix remodeling (MMP14, MMP19 and MMP2) and upregulation of anti-adhesive genes (MUC1) in the cranial regions of uterine horn. Physical proximity to the embryo provides paracrine regulation of endometrial function. Embryo-independent regulation of the endometrial transcriptome may support subsequent stages of embryo development, such as elongation and implantation. We speculate that successful early embryo-dependent and -independent programming fine-tune endometrial functions that are important for maintenance of pregnancy in cattle. |
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Pre-hatching embryo-dependent and -independent programming of endometrial function in cattleThe bovine pre-implantation embryo secretes bioactive molecules from early development stages, but effects on endometrial function are reported to start only after elongation. Here, we interrogated spatially defined regions of the endometrium transcriptome for responses to a day 7 embryo in vivo. We hypothesize that exposure to an embryo changes the abundance of specific transcripts in the cranial region of the pregnant uterine horn. Endometrium was collected from the uterotubal junction (UTJ), anterior (IA), medial (IM) and posterior (IP) regions of the uterine horn ipsilateral to the CL 7 days after estrus from sham-inseminated (Con) or artificially inseminated, confirmed pregnant (Preg) cows. Abundance of 86 transcripts was evaluated by qPCR using a microfluidic platform. Abundance of 12 transcripts was modulated in the Preg endometrium, including classical interferon-stimulated genes (ISG15, MX1, MX2 and OAS1Y), prostaglandin biosynthesis genes (PTGES, HPGD and AKR1C4), water channel (AQP4) and a solute transporter (SLC1A4) and this was in the UTJ and IA mainly. Additionally, for 71 transcripts, abundance varied according to region of the reproductive tract. Regulation included downregulation of genes associated with proliferation (IGF1, IGF2, IGF1R and IGF2R) and extracellular matrix remodeling (MMP14, MMP19 and MMP2) and upregulation of anti-adhesive genes (MUC1) in the cranial regions of uterine horn. Physical proximity to the embryo provides paracrine regulation of endometrial function. Embryo-independent regulation of the endometrial transcriptome may support subsequent stages of embryo development, such as elongation and implantation. We speculate that successful early embryo-dependent and -independent programming fine-tune endometrial functions that are important for maintenance of pregnancy in cattle.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)NSGUniv Sao Paulo, Sch Vet Med & Anim Sci, Pirassununga, SP, BrazilSao Paulo State Univ, Dept Pharmacol, Botucatu, SP, BrazilUniv Sao Paulo, Fac Anim Sci & Food Engn, Dept Vet Med, Pirassununga, SP, BrazilAndrovet, Sertaozinho, SP, BrazilMinas Gerais Fed Univ, Sch Vet, Dept Clin & Surg Vet, Belo Horizonte, MG, BrazilSao Paulo State Univ, Dept Biol Sci, Assis, SP, BrazilSao Paulo State Univ, Dept Pharmacol, Botucatu, SP, BrazilSao Paulo State Univ, Dept Biol Sci, Assis, SP, BrazilFAPESP: 2011/03226-4NSG: 2015/21831-3Public Library ScienceUniversidade de São Paulo (USP)Universidade Estadual Paulista (Unesp)AndrovetMinas Gerais Fed UnivSponchiado, MarianaGomes, Nathalia SouzaFontes, Patricia Kubo [UNESP]Martins, Thiagodel Collado, MaitePastore, Athos de AssumpcaoPugliesi, GuilhermeGouveia Nogueira, Marcelo Fabio [UNESP]Binelli, Mario2018-11-26T17:29:44Z2018-11-26T17:29:44Z2017-04-19info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article23application/pdfhttp://dx.doi.org/10.1371/journal.pone.0175954Plos One. San Francisco: Public Library Science, v. 12, n. 4, 23 p., 2017.1932-6203http://hdl.handle.net/11449/16273810.1371/journal.pone.0175954WOS:000399875600069WOS000399875600069.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPlos One1,164info:eu-repo/semantics/openAccess2024-06-13T17:38:19Zoai:repositorio.unesp.br:11449/162738Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T17:20:07.024910Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Pre-hatching embryo-dependent and -independent programming of endometrial function in cattle |
title |
Pre-hatching embryo-dependent and -independent programming of endometrial function in cattle |
spellingShingle |
Pre-hatching embryo-dependent and -independent programming of endometrial function in cattle Sponchiado, Mariana |
title_short |
Pre-hatching embryo-dependent and -independent programming of endometrial function in cattle |
title_full |
Pre-hatching embryo-dependent and -independent programming of endometrial function in cattle |
title_fullStr |
Pre-hatching embryo-dependent and -independent programming of endometrial function in cattle |
title_full_unstemmed |
Pre-hatching embryo-dependent and -independent programming of endometrial function in cattle |
title_sort |
Pre-hatching embryo-dependent and -independent programming of endometrial function in cattle |
author |
Sponchiado, Mariana |
author_facet |
Sponchiado, Mariana Gomes, Nathalia Souza Fontes, Patricia Kubo [UNESP] Martins, Thiago del Collado, Maite Pastore, Athos de Assumpcao Pugliesi, Guilherme Gouveia Nogueira, Marcelo Fabio [UNESP] Binelli, Mario |
author_role |
author |
author2 |
Gomes, Nathalia Souza Fontes, Patricia Kubo [UNESP] Martins, Thiago del Collado, Maite Pastore, Athos de Assumpcao Pugliesi, Guilherme Gouveia Nogueira, Marcelo Fabio [UNESP] Binelli, Mario |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade de São Paulo (USP) Universidade Estadual Paulista (Unesp) Androvet Minas Gerais Fed Univ |
dc.contributor.author.fl_str_mv |
Sponchiado, Mariana Gomes, Nathalia Souza Fontes, Patricia Kubo [UNESP] Martins, Thiago del Collado, Maite Pastore, Athos de Assumpcao Pugliesi, Guilherme Gouveia Nogueira, Marcelo Fabio [UNESP] Binelli, Mario |
description |
The bovine pre-implantation embryo secretes bioactive molecules from early development stages, but effects on endometrial function are reported to start only after elongation. Here, we interrogated spatially defined regions of the endometrium transcriptome for responses to a day 7 embryo in vivo. We hypothesize that exposure to an embryo changes the abundance of specific transcripts in the cranial region of the pregnant uterine horn. Endometrium was collected from the uterotubal junction (UTJ), anterior (IA), medial (IM) and posterior (IP) regions of the uterine horn ipsilateral to the CL 7 days after estrus from sham-inseminated (Con) or artificially inseminated, confirmed pregnant (Preg) cows. Abundance of 86 transcripts was evaluated by qPCR using a microfluidic platform. Abundance of 12 transcripts was modulated in the Preg endometrium, including classical interferon-stimulated genes (ISG15, MX1, MX2 and OAS1Y), prostaglandin biosynthesis genes (PTGES, HPGD and AKR1C4), water channel (AQP4) and a solute transporter (SLC1A4) and this was in the UTJ and IA mainly. Additionally, for 71 transcripts, abundance varied according to region of the reproductive tract. Regulation included downregulation of genes associated with proliferation (IGF1, IGF2, IGF1R and IGF2R) and extracellular matrix remodeling (MMP14, MMP19 and MMP2) and upregulation of anti-adhesive genes (MUC1) in the cranial regions of uterine horn. Physical proximity to the embryo provides paracrine regulation of endometrial function. Embryo-independent regulation of the endometrial transcriptome may support subsequent stages of embryo development, such as elongation and implantation. We speculate that successful early embryo-dependent and -independent programming fine-tune endometrial functions that are important for maintenance of pregnancy in cattle. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-04-19 2018-11-26T17:29:44Z 2018-11-26T17:29:44Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1371/journal.pone.0175954 Plos One. San Francisco: Public Library Science, v. 12, n. 4, 23 p., 2017. 1932-6203 http://hdl.handle.net/11449/162738 10.1371/journal.pone.0175954 WOS:000399875600069 WOS000399875600069.pdf |
url |
http://dx.doi.org/10.1371/journal.pone.0175954 http://hdl.handle.net/11449/162738 |
identifier_str_mv |
Plos One. San Francisco: Public Library Science, v. 12, n. 4, 23 p., 2017. 1932-6203 10.1371/journal.pone.0175954 WOS:000399875600069 WOS000399875600069.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Plos One 1,164 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
23 application/pdf |
dc.publisher.none.fl_str_mv |
Public Library Science |
publisher.none.fl_str_mv |
Public Library Science |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1808128792516886528 |