Heteropicnotic chromatin and nucleolar activity in meiosis and spermiogenesis of Limnogonus aduncus (Heteroptera, Gerridae): a stained nucleolar organizing region that can serve as a model for studying chromosome behavior.
Autor(a) principal: | |
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Data de Publicação: | 2008 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.4238/vol7-4gmr527 http://hdl.handle.net/11449/70745 |
Resumo: | Males of Limnogonus aduncus were found to have the sex chromosome system X0 and chromosome number 2n = 23 (22A + X0). Testis cells were stained with lacto-acetic orcein and silver nitrate so that changes in the morphology and degree of staining of the heteropicnotic chromatin and the nucleolar material could be observed during meiosis and spermiogenesis. These structures share the same nuclear position and could be seen until almost the end of spermiogenesis. A chromosome region stained with silver nitrate was indicative of a nucleolar organizing region (NOR), which is rarely detected in Heteroptera with this technique. The NOR is located at one end of a single member of an autosome pair. The finding of this stained region enabled us to observe that the telomeric association of sister chromatids that characterizes the Heteroptera does not include the chromosome ends, where NORs are located; we also observed in anaphase that the chromosome end through which it is pulled to the pole is the one containing the NOR. Another observation was that the single nucleolar body present in the cells at anaphase never goes to the cell pole that does not receive the NOR. We conclude that L. aduncus is a good model for cytogenetic studies involving nucleolar activity and also may be useful for studying the mechanisms of activation and inactivation of kinetic activity at the chromosome ends. Although the chromosomes of Heteroptera are known to be holocentric, whether kinetic activity is restricted to one or involves both chromosome ends is still not well understood. |
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Heteropicnotic chromatin and nucleolar activity in meiosis and spermiogenesis of Limnogonus aduncus (Heteroptera, Gerridae): a stained nucleolar organizing region that can serve as a model for studying chromosome behavior.animalbiological modelchromatinchromosomechromosome NORgeneticsHeteropteramalemeiosisnucleolussex chromosomespermatogenesisAnimalsCell NucleolusChromatinChromosomesMaleMeiosisModels, GeneticNucleolus Organizer RegionSex ChromosomesSpermatogenesisMales of Limnogonus aduncus were found to have the sex chromosome system X0 and chromosome number 2n = 23 (22A + X0). Testis cells were stained with lacto-acetic orcein and silver nitrate so that changes in the morphology and degree of staining of the heteropicnotic chromatin and the nucleolar material could be observed during meiosis and spermiogenesis. These structures share the same nuclear position and could be seen until almost the end of spermiogenesis. A chromosome region stained with silver nitrate was indicative of a nucleolar organizing region (NOR), which is rarely detected in Heteroptera with this technique. The NOR is located at one end of a single member of an autosome pair. The finding of this stained region enabled us to observe that the telomeric association of sister chromatids that characterizes the Heteroptera does not include the chromosome ends, where NORs are located; we also observed in anaphase that the chromosome end through which it is pulled to the pole is the one containing the NOR. Another observation was that the single nucleolar body present in the cells at anaphase never goes to the cell pole that does not receive the NOR. We conclude that L. aduncus is a good model for cytogenetic studies involving nucleolar activity and also may be useful for studying the mechanisms of activation and inactivation of kinetic activity at the chromosome ends. Although the chromosomes of Heteroptera are known to be holocentric, whether kinetic activity is restricted to one or involves both chromosome ends is still not well understood.Universidade Estadual Paulista (Unesp)Castanhole, M. M.Pereira, L. L.Souza, H. V.Bicudo, H. E.Costa, L. A.Itoyama, M. M.2014-05-27T11:23:45Z2014-05-27T11:23:45Z2008-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1398-1407application/pdfhttp://dx.doi.org/10.4238/vol7-4gmr527Genetics and molecular research : GMR, v. 7, n. 4, p. 1398-1407, 2008.1676-5680http://hdl.handle.net/11449/7074510.4238/vol7-4gmr5272-s2.0-624492421662-s2.0-62449242166.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengGenetics and molecular research : GMR0,439info:eu-repo/semantics/openAccess2024-01-22T06:24:27Zoai:repositorio.unesp.br:11449/70745Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-01-22T06:24:27Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Heteropicnotic chromatin and nucleolar activity in meiosis and spermiogenesis of Limnogonus aduncus (Heteroptera, Gerridae): a stained nucleolar organizing region that can serve as a model for studying chromosome behavior. |
title |
Heteropicnotic chromatin and nucleolar activity in meiosis and spermiogenesis of Limnogonus aduncus (Heteroptera, Gerridae): a stained nucleolar organizing region that can serve as a model for studying chromosome behavior. |
spellingShingle |
Heteropicnotic chromatin and nucleolar activity in meiosis and spermiogenesis of Limnogonus aduncus (Heteroptera, Gerridae): a stained nucleolar organizing region that can serve as a model for studying chromosome behavior. Castanhole, M. M. animal biological model chromatin chromosome chromosome NOR genetics Heteroptera male meiosis nucleolus sex chromosome spermatogenesis Animals Cell Nucleolus Chromatin Chromosomes Male Meiosis Models, Genetic Nucleolus Organizer Region Sex Chromosomes Spermatogenesis |
title_short |
Heteropicnotic chromatin and nucleolar activity in meiosis and spermiogenesis of Limnogonus aduncus (Heteroptera, Gerridae): a stained nucleolar organizing region that can serve as a model for studying chromosome behavior. |
title_full |
Heteropicnotic chromatin and nucleolar activity in meiosis and spermiogenesis of Limnogonus aduncus (Heteroptera, Gerridae): a stained nucleolar organizing region that can serve as a model for studying chromosome behavior. |
title_fullStr |
Heteropicnotic chromatin and nucleolar activity in meiosis and spermiogenesis of Limnogonus aduncus (Heteroptera, Gerridae): a stained nucleolar organizing region that can serve as a model for studying chromosome behavior. |
title_full_unstemmed |
Heteropicnotic chromatin and nucleolar activity in meiosis and spermiogenesis of Limnogonus aduncus (Heteroptera, Gerridae): a stained nucleolar organizing region that can serve as a model for studying chromosome behavior. |
title_sort |
Heteropicnotic chromatin and nucleolar activity in meiosis and spermiogenesis of Limnogonus aduncus (Heteroptera, Gerridae): a stained nucleolar organizing region that can serve as a model for studying chromosome behavior. |
author |
Castanhole, M. M. |
author_facet |
Castanhole, M. M. Pereira, L. L. Souza, H. V. Bicudo, H. E. Costa, L. A. Itoyama, M. M. |
author_role |
author |
author2 |
Pereira, L. L. Souza, H. V. Bicudo, H. E. Costa, L. A. Itoyama, M. M. |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Castanhole, M. M. Pereira, L. L. Souza, H. V. Bicudo, H. E. Costa, L. A. Itoyama, M. M. |
dc.subject.por.fl_str_mv |
animal biological model chromatin chromosome chromosome NOR genetics Heteroptera male meiosis nucleolus sex chromosome spermatogenesis Animals Cell Nucleolus Chromatin Chromosomes Male Meiosis Models, Genetic Nucleolus Organizer Region Sex Chromosomes Spermatogenesis |
topic |
animal biological model chromatin chromosome chromosome NOR genetics Heteroptera male meiosis nucleolus sex chromosome spermatogenesis Animals Cell Nucleolus Chromatin Chromosomes Male Meiosis Models, Genetic Nucleolus Organizer Region Sex Chromosomes Spermatogenesis |
description |
Males of Limnogonus aduncus were found to have the sex chromosome system X0 and chromosome number 2n = 23 (22A + X0). Testis cells were stained with lacto-acetic orcein and silver nitrate so that changes in the morphology and degree of staining of the heteropicnotic chromatin and the nucleolar material could be observed during meiosis and spermiogenesis. These structures share the same nuclear position and could be seen until almost the end of spermiogenesis. A chromosome region stained with silver nitrate was indicative of a nucleolar organizing region (NOR), which is rarely detected in Heteroptera with this technique. The NOR is located at one end of a single member of an autosome pair. The finding of this stained region enabled us to observe that the telomeric association of sister chromatids that characterizes the Heteroptera does not include the chromosome ends, where NORs are located; we also observed in anaphase that the chromosome end through which it is pulled to the pole is the one containing the NOR. Another observation was that the single nucleolar body present in the cells at anaphase never goes to the cell pole that does not receive the NOR. We conclude that L. aduncus is a good model for cytogenetic studies involving nucleolar activity and also may be useful for studying the mechanisms of activation and inactivation of kinetic activity at the chromosome ends. Although the chromosomes of Heteroptera are known to be holocentric, whether kinetic activity is restricted to one or involves both chromosome ends is still not well understood. |
publishDate |
2008 |
dc.date.none.fl_str_mv |
2008-12-01 2014-05-27T11:23:45Z 2014-05-27T11:23:45Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.4238/vol7-4gmr527 Genetics and molecular research : GMR, v. 7, n. 4, p. 1398-1407, 2008. 1676-5680 http://hdl.handle.net/11449/70745 10.4238/vol7-4gmr527 2-s2.0-62449242166 2-s2.0-62449242166.pdf |
url |
http://dx.doi.org/10.4238/vol7-4gmr527 http://hdl.handle.net/11449/70745 |
identifier_str_mv |
Genetics and molecular research : GMR, v. 7, n. 4, p. 1398-1407, 2008. 1676-5680 10.4238/vol7-4gmr527 2-s2.0-62449242166 2-s2.0-62449242166.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Genetics and molecular research : GMR 0,439 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1398-1407 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1803047398899974144 |