Dietary protein-to-carbohydrate ratios affect metabolism and growth of juvenile surubim cachara (Pseudoplatystoma reticulatum)
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1007/s10499-017-0213-3 http://hdl.handle.net/11449/175503 |
Resumo: | This study was conducted to evaluate the effects of dietary carbohydrates (CHO) and protein on growth performance, nutrient retention, blood metabolites, tissue energy reserves (hepatic glycogen and lipids, mesenteric fat index, and hepatosomatic index), and key metabolic enzyme activities (hexokinase, glucokinase, glucose-6-phosphate dehydrogenase, and aspartate aminotransferase) of surubim cachara (Pseudoplatystoma reticulatum). Six experimental diets containing one of two CHO levels (170 or 300 g kg−1) and three crude protein (CP) levels (360, 400, or 440 g kg−1) were fed to four replicate groups of P. reticulatum (69.2 ± 4.4 g) for 60 days. The results showed that 300 g kg−1 CHO resulted in a worse final weight (FW, 208.4 ± 26.9 g), weight gain (WG, 139.4 ± 23.9 g), specific growth rate (SGR, 1.8 ± 0.2% day−1), apparent feed conversion (AFC, 1.5 ± 0.3), and protein efficiency ratio (PER, 163.7 ± 23.4%) than fish fed 170 g kg−1 CHO (FW, 241.4 ± 37.3 g; WG, 171.9 ± 36.6 g; SGR, 2.1 ± 0.2% day−1; AFC, 1.2 ± 0.2; PER, 203.7 ± 36.9%). Fish fed 170 g kg−1 CHO showed greater retention of CP (REcp, 36.3 ± 6.9%) and energy (REge, 23.9 ± 5.6%) in their carcass than fish fed 300 g kg−1 CHO (REcp, 28.9 ± 3.9%, REge, 18.5 ± 3.7%). The highest blood glucose levels (97.7 ± 32.7 mg dL−1) were observed in fish fed the 300CHO/400CP diet, and they had a significantly lower mesenteric fat index (0.85 ± 0.26%) than fish fed with the 170CHO/400CP diet (1.25 ± 0.33%). Hepatic hexokinase activity increased in fish fed 300 g kg−1 CHO, resulting in an activity of 5.0 μmol mg−1 of protein, whereas fish fed 170 g kg−1 CHO had an activity of 3.2 μmol mg−1 of protein. These results demonstrate that dietary CHO directly affect the intermediary metabolism of P. reticulatum and feeding 300 g kg−1 CHO and 440 g kg−1 CP should be considered excessive as it results in decreased nutrient retention in the carcass and decreased growth performance despite metabolic adjustments. |
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Dietary protein-to-carbohydrate ratios affect metabolism and growth of juvenile surubim cachara (Pseudoplatystoma reticulatum)Dietary starchFeed formulationGrowthHepatic enzymesThis study was conducted to evaluate the effects of dietary carbohydrates (CHO) and protein on growth performance, nutrient retention, blood metabolites, tissue energy reserves (hepatic glycogen and lipids, mesenteric fat index, and hepatosomatic index), and key metabolic enzyme activities (hexokinase, glucokinase, glucose-6-phosphate dehydrogenase, and aspartate aminotransferase) of surubim cachara (Pseudoplatystoma reticulatum). Six experimental diets containing one of two CHO levels (170 or 300 g kg−1) and three crude protein (CP) levels (360, 400, or 440 g kg−1) were fed to four replicate groups of P. reticulatum (69.2 ± 4.4 g) for 60 days. The results showed that 300 g kg−1 CHO resulted in a worse final weight (FW, 208.4 ± 26.9 g), weight gain (WG, 139.4 ± 23.9 g), specific growth rate (SGR, 1.8 ± 0.2% day−1), apparent feed conversion (AFC, 1.5 ± 0.3), and protein efficiency ratio (PER, 163.7 ± 23.4%) than fish fed 170 g kg−1 CHO (FW, 241.4 ± 37.3 g; WG, 171.9 ± 36.6 g; SGR, 2.1 ± 0.2% day−1; AFC, 1.2 ± 0.2; PER, 203.7 ± 36.9%). Fish fed 170 g kg−1 CHO showed greater retention of CP (REcp, 36.3 ± 6.9%) and energy (REge, 23.9 ± 5.6%) in their carcass than fish fed 300 g kg−1 CHO (REcp, 28.9 ± 3.9%, REge, 18.5 ± 3.7%). The highest blood glucose levels (97.7 ± 32.7 mg dL−1) were observed in fish fed the 300CHO/400CP diet, and they had a significantly lower mesenteric fat index (0.85 ± 0.26%) than fish fed with the 170CHO/400CP diet (1.25 ± 0.33%). Hepatic hexokinase activity increased in fish fed 300 g kg−1 CHO, resulting in an activity of 5.0 μmol mg−1 of protein, whereas fish fed 170 g kg−1 CHO had an activity of 3.2 μmol mg−1 of protein. These results demonstrate that dietary CHO directly affect the intermediary metabolism of P. reticulatum and feeding 300 g kg−1 CHO and 440 g kg−1 CP should be considered excessive as it results in decreased nutrient retention in the carcass and decreased growth performance despite metabolic adjustments.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)College of Agricultural and Technological Sciences (FCAT) São Paulo State University (UNESP), Rod. Comte. João Ribeiro de Barros, km 651, DracenaAquaculture Center of Unesp (Caunesp) São Paulo State University (UNESP), Via de Ac. Prof. Paulo Donato Castellane, s/n, JaboticabalCollege of Agricultural and Technological Sciences (FCAT) São Paulo State University (UNESP), Rod. Comte. João Ribeiro de Barros, km 651, DracenaAquaculture Center of Unesp (Caunesp) São Paulo State University (UNESP), Via de Ac. Prof. Paulo Donato Castellane, s/n, JaboticabalFAPESP: 2012/13526-8Universidade Estadual Paulista (Unesp)Gonçalves, André Fernando Nascimento [UNESP]Ha, Natalia [UNESP]Biller-Takahashi, Jaqueline Dalbello [UNESP]Gimbo, Rodrigo Yukihiro [UNESP]Urbinati, Elisabeth Criscuolo [UNESP]Takahashi, Leonardo Susumu [UNESP]2018-12-11T17:16:05Z2018-12-11T17:16:05Z2018-02-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article349-362application/pdfhttp://dx.doi.org/10.1007/s10499-017-0213-3Aquaculture International, v. 26, n. 1, p. 349-362, 2018.1573-143X0967-6120http://hdl.handle.net/11449/17550310.1007/s10499-017-0213-32-s2.0-850342702672-s2.0-85034270267.pdf3860915077534516Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengAquaculture International0,591info:eu-repo/semantics/openAccess2024-04-09T15:29:36Zoai:repositorio.unesp.br:11449/175503Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T16:39:17.693441Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Dietary protein-to-carbohydrate ratios affect metabolism and growth of juvenile surubim cachara (Pseudoplatystoma reticulatum) |
title |
Dietary protein-to-carbohydrate ratios affect metabolism and growth of juvenile surubim cachara (Pseudoplatystoma reticulatum) |
spellingShingle |
Dietary protein-to-carbohydrate ratios affect metabolism and growth of juvenile surubim cachara (Pseudoplatystoma reticulatum) Gonçalves, André Fernando Nascimento [UNESP] Dietary starch Feed formulation Growth Hepatic enzymes |
title_short |
Dietary protein-to-carbohydrate ratios affect metabolism and growth of juvenile surubim cachara (Pseudoplatystoma reticulatum) |
title_full |
Dietary protein-to-carbohydrate ratios affect metabolism and growth of juvenile surubim cachara (Pseudoplatystoma reticulatum) |
title_fullStr |
Dietary protein-to-carbohydrate ratios affect metabolism and growth of juvenile surubim cachara (Pseudoplatystoma reticulatum) |
title_full_unstemmed |
Dietary protein-to-carbohydrate ratios affect metabolism and growth of juvenile surubim cachara (Pseudoplatystoma reticulatum) |
title_sort |
Dietary protein-to-carbohydrate ratios affect metabolism and growth of juvenile surubim cachara (Pseudoplatystoma reticulatum) |
author |
Gonçalves, André Fernando Nascimento [UNESP] |
author_facet |
Gonçalves, André Fernando Nascimento [UNESP] Ha, Natalia [UNESP] Biller-Takahashi, Jaqueline Dalbello [UNESP] Gimbo, Rodrigo Yukihiro [UNESP] Urbinati, Elisabeth Criscuolo [UNESP] Takahashi, Leonardo Susumu [UNESP] |
author_role |
author |
author2 |
Ha, Natalia [UNESP] Biller-Takahashi, Jaqueline Dalbello [UNESP] Gimbo, Rodrigo Yukihiro [UNESP] Urbinati, Elisabeth Criscuolo [UNESP] Takahashi, Leonardo Susumu [UNESP] |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Gonçalves, André Fernando Nascimento [UNESP] Ha, Natalia [UNESP] Biller-Takahashi, Jaqueline Dalbello [UNESP] Gimbo, Rodrigo Yukihiro [UNESP] Urbinati, Elisabeth Criscuolo [UNESP] Takahashi, Leonardo Susumu [UNESP] |
dc.subject.por.fl_str_mv |
Dietary starch Feed formulation Growth Hepatic enzymes |
topic |
Dietary starch Feed formulation Growth Hepatic enzymes |
description |
This study was conducted to evaluate the effects of dietary carbohydrates (CHO) and protein on growth performance, nutrient retention, blood metabolites, tissue energy reserves (hepatic glycogen and lipids, mesenteric fat index, and hepatosomatic index), and key metabolic enzyme activities (hexokinase, glucokinase, glucose-6-phosphate dehydrogenase, and aspartate aminotransferase) of surubim cachara (Pseudoplatystoma reticulatum). Six experimental diets containing one of two CHO levels (170 or 300 g kg−1) and three crude protein (CP) levels (360, 400, or 440 g kg−1) were fed to four replicate groups of P. reticulatum (69.2 ± 4.4 g) for 60 days. The results showed that 300 g kg−1 CHO resulted in a worse final weight (FW, 208.4 ± 26.9 g), weight gain (WG, 139.4 ± 23.9 g), specific growth rate (SGR, 1.8 ± 0.2% day−1), apparent feed conversion (AFC, 1.5 ± 0.3), and protein efficiency ratio (PER, 163.7 ± 23.4%) than fish fed 170 g kg−1 CHO (FW, 241.4 ± 37.3 g; WG, 171.9 ± 36.6 g; SGR, 2.1 ± 0.2% day−1; AFC, 1.2 ± 0.2; PER, 203.7 ± 36.9%). Fish fed 170 g kg−1 CHO showed greater retention of CP (REcp, 36.3 ± 6.9%) and energy (REge, 23.9 ± 5.6%) in their carcass than fish fed 300 g kg−1 CHO (REcp, 28.9 ± 3.9%, REge, 18.5 ± 3.7%). The highest blood glucose levels (97.7 ± 32.7 mg dL−1) were observed in fish fed the 300CHO/400CP diet, and they had a significantly lower mesenteric fat index (0.85 ± 0.26%) than fish fed with the 170CHO/400CP diet (1.25 ± 0.33%). Hepatic hexokinase activity increased in fish fed 300 g kg−1 CHO, resulting in an activity of 5.0 μmol mg−1 of protein, whereas fish fed 170 g kg−1 CHO had an activity of 3.2 μmol mg−1 of protein. These results demonstrate that dietary CHO directly affect the intermediary metabolism of P. reticulatum and feeding 300 g kg−1 CHO and 440 g kg−1 CP should be considered excessive as it results in decreased nutrient retention in the carcass and decreased growth performance despite metabolic adjustments. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-12-11T17:16:05Z 2018-12-11T17:16:05Z 2018-02-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/s10499-017-0213-3 Aquaculture International, v. 26, n. 1, p. 349-362, 2018. 1573-143X 0967-6120 http://hdl.handle.net/11449/175503 10.1007/s10499-017-0213-3 2-s2.0-85034270267 2-s2.0-85034270267.pdf 3860915077534516 |
url |
http://dx.doi.org/10.1007/s10499-017-0213-3 http://hdl.handle.net/11449/175503 |
identifier_str_mv |
Aquaculture International, v. 26, n. 1, p. 349-362, 2018. 1573-143X 0967-6120 10.1007/s10499-017-0213-3 2-s2.0-85034270267 2-s2.0-85034270267.pdf 3860915077534516 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Aquaculture International 0,591 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
349-362 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128684174868480 |