Evaluation of a biosensor based on reduced graphene oxide and glucose oxidase enzyme on the monitoring of second-generation ethanol production
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1007/s10008-019-04471-7 http://hdl.handle.net/11449/198312 |
Resumo: | An easily prepared biosensor based on reduced graphene oxide (rGO) and glucose oxidase (GOx) enzyme was developed to monitor the enzymatic hydrolysis process of the second-generation (2G) ethanol process from green coconut biomass. The rGO-GOx biocomposite that modified a glassy carbon (GC) electrode was characterized by morphological, electrochemical and spectrophotometric techniques showing that the GOx enzyme was immobilized on the rGO. The parameters for glucose determination were optimized by square wave voltammetry (SWV). The developed biosensor was applied for the determination of glucose during the enzymatic hydrolysis step, showing that the process can be stopped with 12 h of reaction. Thus, an important achievement of this analysis is the reduced time to get a valuable result for the test, saving time and reducing the cost of the 2G ethanol process. |
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Repositório Institucional da UNESP |
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Evaluation of a biosensor based on reduced graphene oxide and glucose oxidase enzyme on the monitoring of second-generation ethanol production2G ethanolBiosensorGlucoseGrapheneAn easily prepared biosensor based on reduced graphene oxide (rGO) and glucose oxidase (GOx) enzyme was developed to monitor the enzymatic hydrolysis process of the second-generation (2G) ethanol process from green coconut biomass. The rGO-GOx biocomposite that modified a glassy carbon (GC) electrode was characterized by morphological, electrochemical and spectrophotometric techniques showing that the GOx enzyme was immobilized on the rGO. The parameters for glucose determination were optimized by square wave voltammetry (SWV). The developed biosensor was applied for the determination of glucose during the enzymatic hydrolysis step, showing that the process can be stopped with 12 h of reaction. Thus, an important achievement of this analysis is the reduced time to get a valuable result for the test, saving time and reducing the cost of the 2G ethanol process.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)School of Agriculture São Paulo State University (UNESP), Avenida Universitária, no 3780School of Agriculture São Paulo State University (UNESP), Avenida Universitária, no 3780FAPESP: 2017/03925-6FAPESP: 2017/24274-3Universidade Estadual Paulista (Unesp)Donini, Caruane A. [UNESP]Silva, Martin K. L. [UNESP]Bronzato, Giovana R. [UNESP]Leão, Alcides L. [UNESP]Cesarino, Ivana [UNESP]2020-12-12T01:09:20Z2020-12-12T01:09:20Z2020-08-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article2011-2018http://dx.doi.org/10.1007/s10008-019-04471-7Journal of Solid State Electrochemistry, v. 24, n. 8, p. 2011-2018, 2020.1433-07681432-8488http://hdl.handle.net/11449/19831210.1007/s10008-019-04471-72-s2.0-85076915484Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Solid State Electrochemistryinfo:eu-repo/semantics/openAccess2021-10-23T08:53:37Zoai:repositorio.unesp.br:11449/198312Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-23T08:53:37Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Evaluation of a biosensor based on reduced graphene oxide and glucose oxidase enzyme on the monitoring of second-generation ethanol production |
title |
Evaluation of a biosensor based on reduced graphene oxide and glucose oxidase enzyme on the monitoring of second-generation ethanol production |
spellingShingle |
Evaluation of a biosensor based on reduced graphene oxide and glucose oxidase enzyme on the monitoring of second-generation ethanol production Donini, Caruane A. [UNESP] 2G ethanol Biosensor Glucose Graphene |
title_short |
Evaluation of a biosensor based on reduced graphene oxide and glucose oxidase enzyme on the monitoring of second-generation ethanol production |
title_full |
Evaluation of a biosensor based on reduced graphene oxide and glucose oxidase enzyme on the monitoring of second-generation ethanol production |
title_fullStr |
Evaluation of a biosensor based on reduced graphene oxide and glucose oxidase enzyme on the monitoring of second-generation ethanol production |
title_full_unstemmed |
Evaluation of a biosensor based on reduced graphene oxide and glucose oxidase enzyme on the monitoring of second-generation ethanol production |
title_sort |
Evaluation of a biosensor based on reduced graphene oxide and glucose oxidase enzyme on the monitoring of second-generation ethanol production |
author |
Donini, Caruane A. [UNESP] |
author_facet |
Donini, Caruane A. [UNESP] Silva, Martin K. L. [UNESP] Bronzato, Giovana R. [UNESP] Leão, Alcides L. [UNESP] Cesarino, Ivana [UNESP] |
author_role |
author |
author2 |
Silva, Martin K. L. [UNESP] Bronzato, Giovana R. [UNESP] Leão, Alcides L. [UNESP] Cesarino, Ivana [UNESP] |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Donini, Caruane A. [UNESP] Silva, Martin K. L. [UNESP] Bronzato, Giovana R. [UNESP] Leão, Alcides L. [UNESP] Cesarino, Ivana [UNESP] |
dc.subject.por.fl_str_mv |
2G ethanol Biosensor Glucose Graphene |
topic |
2G ethanol Biosensor Glucose Graphene |
description |
An easily prepared biosensor based on reduced graphene oxide (rGO) and glucose oxidase (GOx) enzyme was developed to monitor the enzymatic hydrolysis process of the second-generation (2G) ethanol process from green coconut biomass. The rGO-GOx biocomposite that modified a glassy carbon (GC) electrode was characterized by morphological, electrochemical and spectrophotometric techniques showing that the GOx enzyme was immobilized on the rGO. The parameters for glucose determination were optimized by square wave voltammetry (SWV). The developed biosensor was applied for the determination of glucose during the enzymatic hydrolysis step, showing that the process can be stopped with 12 h of reaction. Thus, an important achievement of this analysis is the reduced time to get a valuable result for the test, saving time and reducing the cost of the 2G ethanol process. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-12T01:09:20Z 2020-12-12T01:09:20Z 2020-08-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/s10008-019-04471-7 Journal of Solid State Electrochemistry, v. 24, n. 8, p. 2011-2018, 2020. 1433-0768 1432-8488 http://hdl.handle.net/11449/198312 10.1007/s10008-019-04471-7 2-s2.0-85076915484 |
url |
http://dx.doi.org/10.1007/s10008-019-04471-7 http://hdl.handle.net/11449/198312 |
identifier_str_mv |
Journal of Solid State Electrochemistry, v. 24, n. 8, p. 2011-2018, 2020. 1433-0768 1432-8488 10.1007/s10008-019-04471-7 2-s2.0-85076915484 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Solid State Electrochemistry |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
2011-2018 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799964450963324928 |