Characterization of semen collected by pharmacologically induced ejaculation from a stallion with seminal vesiculitis

Detalhes bibliográficos
Autor(a) principal: Cavalero, Thaís Mendes Sanches [UNESP]
Data de Publicação: 2020
Outros Autores: Segabinazzi, Lorenzo Garrido Teixeira M. [UNESP], Scheeren, Verônica Flores da Cunha [UNESP], Freitas-Dell'Aqua, Camila P. [UNESP], Papa, Frederico Ozanam [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1111/rda.13860
http://hdl.handle.net/11449/208188
Resumo: The present study compared the quality of sperm collected by artificial vagina or pharmacologically induced ejaculation from a 10-year-old thoroughbred stallion with seminal vesiculitis. The pharmacological protocol involved intravenous administration of detomidine (0.01 mg/kg) and oxytocin (20 IU) and successfully induced ejaculation in all attempts of semen collection. Sperm motility, plasma membrane and acrosome integrity (PMAI), reactive oxygen species (ROS) levels, polymorphonuclear neutrophil (PMN) percentage, and bacterial profiles of fresh and cooled semen (5°C for 24 hr) were evaluated. Semen obtained by the pharmacological method presented reduced seminal volume, decreased PMN percentage and superior sperm motility in cooled samples. Moreover, higher PMAI and lower ROS levels were observed in semen collected by the pharmacological method. Therefore, pharmacologically induced ejaculation is an alternative to obtain semen with minimal contamination and with sperm of superior quality and longevity from stallions with seminal vesiculitis.
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spelling Characterization of semen collected by pharmacologically induced ejaculation from a stallion with seminal vesiculitischemical ejaculationoxidative stresspyospermiaROSThe present study compared the quality of sperm collected by artificial vagina or pharmacologically induced ejaculation from a 10-year-old thoroughbred stallion with seminal vesiculitis. The pharmacological protocol involved intravenous administration of detomidine (0.01 mg/kg) and oxytocin (20 IU) and successfully induced ejaculation in all attempts of semen collection. Sperm motility, plasma membrane and acrosome integrity (PMAI), reactive oxygen species (ROS) levels, polymorphonuclear neutrophil (PMN) percentage, and bacterial profiles of fresh and cooled semen (5°C for 24 hr) were evaluated. Semen obtained by the pharmacological method presented reduced seminal volume, decreased PMN percentage and superior sperm motility in cooled samples. Moreover, higher PMAI and lower ROS levels were observed in semen collected by the pharmacological method. Therefore, pharmacologically induced ejaculation is an alternative to obtain semen with minimal contamination and with sperm of superior quality and longevity from stallions with seminal vesiculitis.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)School of Veterinary Medicine and Animal Science Sao Paulo State University (UNESP)School of Veterinary Medicine and Animal Science Sao Paulo State University (UNESP)FAPESP: 2016/21452-5Universidade Estadual Paulista (Unesp)Cavalero, Thaís Mendes Sanches [UNESP]Segabinazzi, Lorenzo Garrido Teixeira M. [UNESP]Scheeren, Verônica Flores da Cunha [UNESP]Freitas-Dell'Aqua, Camila P. [UNESP]Papa, Frederico Ozanam [UNESP]2021-06-25T11:07:56Z2021-06-25T11:07:56Z2020-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1808-1811http://dx.doi.org/10.1111/rda.13860Reproduction in Domestic Animals, v. 55, n. 12, p. 1808-1811, 2020.1439-05310936-6768http://hdl.handle.net/11449/20818810.1111/rda.138602-s2.0-85096722032Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengReproduction in Domestic Animalsinfo:eu-repo/semantics/openAccess2024-09-09T14:01:20Zoai:repositorio.unesp.br:11449/208188Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-09T14:01:20Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Characterization of semen collected by pharmacologically induced ejaculation from a stallion with seminal vesiculitis
title Characterization of semen collected by pharmacologically induced ejaculation from a stallion with seminal vesiculitis
spellingShingle Characterization of semen collected by pharmacologically induced ejaculation from a stallion with seminal vesiculitis
Cavalero, Thaís Mendes Sanches [UNESP]
chemical ejaculation
oxidative stress
pyospermia
ROS
title_short Characterization of semen collected by pharmacologically induced ejaculation from a stallion with seminal vesiculitis
title_full Characterization of semen collected by pharmacologically induced ejaculation from a stallion with seminal vesiculitis
title_fullStr Characterization of semen collected by pharmacologically induced ejaculation from a stallion with seminal vesiculitis
title_full_unstemmed Characterization of semen collected by pharmacologically induced ejaculation from a stallion with seminal vesiculitis
title_sort Characterization of semen collected by pharmacologically induced ejaculation from a stallion with seminal vesiculitis
author Cavalero, Thaís Mendes Sanches [UNESP]
author_facet Cavalero, Thaís Mendes Sanches [UNESP]
Segabinazzi, Lorenzo Garrido Teixeira M. [UNESP]
Scheeren, Verônica Flores da Cunha [UNESP]
Freitas-Dell'Aqua, Camila P. [UNESP]
Papa, Frederico Ozanam [UNESP]
author_role author
author2 Segabinazzi, Lorenzo Garrido Teixeira M. [UNESP]
Scheeren, Verônica Flores da Cunha [UNESP]
Freitas-Dell'Aqua, Camila P. [UNESP]
Papa, Frederico Ozanam [UNESP]
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Cavalero, Thaís Mendes Sanches [UNESP]
Segabinazzi, Lorenzo Garrido Teixeira M. [UNESP]
Scheeren, Verônica Flores da Cunha [UNESP]
Freitas-Dell'Aqua, Camila P. [UNESP]
Papa, Frederico Ozanam [UNESP]
dc.subject.por.fl_str_mv chemical ejaculation
oxidative stress
pyospermia
ROS
topic chemical ejaculation
oxidative stress
pyospermia
ROS
description The present study compared the quality of sperm collected by artificial vagina or pharmacologically induced ejaculation from a 10-year-old thoroughbred stallion with seminal vesiculitis. The pharmacological protocol involved intravenous administration of detomidine (0.01 mg/kg) and oxytocin (20 IU) and successfully induced ejaculation in all attempts of semen collection. Sperm motility, plasma membrane and acrosome integrity (PMAI), reactive oxygen species (ROS) levels, polymorphonuclear neutrophil (PMN) percentage, and bacterial profiles of fresh and cooled semen (5°C for 24 hr) were evaluated. Semen obtained by the pharmacological method presented reduced seminal volume, decreased PMN percentage and superior sperm motility in cooled samples. Moreover, higher PMAI and lower ROS levels were observed in semen collected by the pharmacological method. Therefore, pharmacologically induced ejaculation is an alternative to obtain semen with minimal contamination and with sperm of superior quality and longevity from stallions with seminal vesiculitis.
publishDate 2020
dc.date.none.fl_str_mv 2020-12-01
2021-06-25T11:07:56Z
2021-06-25T11:07:56Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1111/rda.13860
Reproduction in Domestic Animals, v. 55, n. 12, p. 1808-1811, 2020.
1439-0531
0936-6768
http://hdl.handle.net/11449/208188
10.1111/rda.13860
2-s2.0-85096722032
url http://dx.doi.org/10.1111/rda.13860
http://hdl.handle.net/11449/208188
identifier_str_mv Reproduction in Domestic Animals, v. 55, n. 12, p. 1808-1811, 2020.
1439-0531
0936-6768
10.1111/rda.13860
2-s2.0-85096722032
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Reproduction in Domestic Animals
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 1808-1811
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv repositoriounesp@unesp.br
_version_ 1813546575998222336

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