Purification, biochemical and functional characterization of miliin, a new thiol-dependent serine protease isolated from the latex of Euphorbia milii
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2008 |
| Outros Autores: | , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da UNESP |
| Texto Completo: | http://dx.doi.org/10.2174/092986608785133744 http://hdl.handle.net/11449/225247 |
Resumo: | Miliin, a new thiol-dependent serine protease purified from the latex of Euphorbia milii possesses a molecular weight of 79 kDa, an isoelectric point of 4.3 and is optimally active at 60°C in the pH range of and 7.5-11.0. Activity tests indicate that milliin is a thiol-dependent serine protease. © 2008 Bentham Science Publishers Ltd. |
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Purification, biochemical and functional characterization of miliin, a new thiol-dependent serine protease isolated from the latex of Euphorbia miliiCharacterizationEuphorbia miliiLatexMedicinal plantPurificationSerine proteaseMiliin, a new thiol-dependent serine protease purified from the latex of Euphorbia milii possesses a molecular weight of 79 kDa, an isoelectric point of 4.3 and is optimally active at 60°C in the pH range of and 7.5-11.0. Activity tests indicate that milliin is a thiol-dependent serine protease. © 2008 Bentham Science Publishers Ltd.Molecular Biophysics Graduate Program IBILCE-UNESP, Campinas, SPCenter for Structural Molecular Biology Brazilian Synchrotron Light Laboratory, Campinas, SPFaculty of Pharmacy University of São Paulo, Ribeirão Preto, SPDepartment of Molecular Biology FAMERP, São José do Rio Preto, SPDepartment of Physics IBILCE-UNESP, São PauloDepartment of Biophysics UNIFESP, São PauloDepartment of Chemistry and Environmental Sciences IBILCE-UNESP, São José do Rio Preto SPDQCA IBILCE-UNESP, Rua Cristovão Colombo 2265, São José do Rio Preto SP, CEP 15054-000Molecular Biophysics Graduate Program IBILCE-UNESP, Campinas, SPDepartment of Physics IBILCE-UNESP, São PauloDepartment of Chemistry and Environmental Sciences IBILCE-UNESP, São José do Rio Preto SPDQCA IBILCE-UNESP, Rua Cristovão Colombo 2265, São José do Rio Preto SP, CEP 15054-000Universidade Estadual Paulista (UNESP)LaboratoryUniversidade de São Paulo (USP)FAMERPUniversidade Federal de São Paulo (UNIFESP)Moro, L. P. [UNESP]Murakami, M. T.Cabral, H.Vidotto, A.Tajara, E. H.Arni, R. K. [UNESP]Juliano, L.Bonilla-Rodriguez, G. O. [UNESP]2022-04-28T20:43:08Z2022-04-28T20:43:08Z2008-07-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article724-730http://dx.doi.org/10.2174/092986608785133744Protein and Peptide Letters, v. 15, n. 7, p. 724-730, 2008.0929-8665http://hdl.handle.net/11449/22524710.2174/0929866087851337442-s2.0-49449115536Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengProtein and Peptide Lettersinfo:eu-repo/semantics/openAccess2022-04-28T20:43:09Zoai:repositorio.unesp.br:11449/225247Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T19:36:05.710830Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
| dc.title.none.fl_str_mv |
Purification, biochemical and functional characterization of miliin, a new thiol-dependent serine protease isolated from the latex of Euphorbia milii |
| title |
Purification, biochemical and functional characterization of miliin, a new thiol-dependent serine protease isolated from the latex of Euphorbia milii |
| spellingShingle |
Purification, biochemical and functional characterization of miliin, a new thiol-dependent serine protease isolated from the latex of Euphorbia milii Moro, L. P. [UNESP] Characterization Euphorbia milii Latex Medicinal plant Purification Serine protease |
| title_short |
Purification, biochemical and functional characterization of miliin, a new thiol-dependent serine protease isolated from the latex of Euphorbia milii |
| title_full |
Purification, biochemical and functional characterization of miliin, a new thiol-dependent serine protease isolated from the latex of Euphorbia milii |
| title_fullStr |
Purification, biochemical and functional characterization of miliin, a new thiol-dependent serine protease isolated from the latex of Euphorbia milii |
| title_full_unstemmed |
Purification, biochemical and functional characterization of miliin, a new thiol-dependent serine protease isolated from the latex of Euphorbia milii |
| title_sort |
Purification, biochemical and functional characterization of miliin, a new thiol-dependent serine protease isolated from the latex of Euphorbia milii |
| author |
Moro, L. P. [UNESP] |
| author_facet |
Moro, L. P. [UNESP] Murakami, M. T. Cabral, H. Vidotto, A. Tajara, E. H. Arni, R. K. [UNESP] Juliano, L. Bonilla-Rodriguez, G. O. [UNESP] |
| author_role |
author |
| author2 |
Murakami, M. T. Cabral, H. Vidotto, A. Tajara, E. H. Arni, R. K. [UNESP] Juliano, L. Bonilla-Rodriguez, G. O. [UNESP] |
| author2_role |
author author author author author author author |
| dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) Laboratory Universidade de São Paulo (USP) FAMERP Universidade Federal de São Paulo (UNIFESP) |
| dc.contributor.author.fl_str_mv |
Moro, L. P. [UNESP] Murakami, M. T. Cabral, H. Vidotto, A. Tajara, E. H. Arni, R. K. [UNESP] Juliano, L. Bonilla-Rodriguez, G. O. [UNESP] |
| dc.subject.por.fl_str_mv |
Characterization Euphorbia milii Latex Medicinal plant Purification Serine protease |
| topic |
Characterization Euphorbia milii Latex Medicinal plant Purification Serine protease |
| description |
Miliin, a new thiol-dependent serine protease purified from the latex of Euphorbia milii possesses a molecular weight of 79 kDa, an isoelectric point of 4.3 and is optimally active at 60°C in the pH range of and 7.5-11.0. Activity tests indicate that milliin is a thiol-dependent serine protease. © 2008 Bentham Science Publishers Ltd. |
| publishDate |
2008 |
| dc.date.none.fl_str_mv |
2008-07-01 2022-04-28T20:43:08Z 2022-04-28T20:43:08Z |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.2174/092986608785133744 Protein and Peptide Letters, v. 15, n. 7, p. 724-730, 2008. 0929-8665 http://hdl.handle.net/11449/225247 10.2174/092986608785133744 2-s2.0-49449115536 |
| url |
http://dx.doi.org/10.2174/092986608785133744 http://hdl.handle.net/11449/225247 |
| identifier_str_mv |
Protein and Peptide Letters, v. 15, n. 7, p. 724-730, 2008. 0929-8665 10.2174/092986608785133744 2-s2.0-49449115536 |
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eng |
| language |
eng |
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Protein and Peptide Letters |
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info:eu-repo/semantics/openAccess |
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openAccess |
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724-730 |
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Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
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Universidade Estadual Paulista (UNESP) |
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UNESP |
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UNESP |
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Repositório Institucional da UNESP |
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Repositório Institucional da UNESP |
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Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
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1808129094443859968 |