The use of real-time PCR (qPCR) for the diagnosis of bovine herpes virus 5 in formalin-fixed, paraffin-embedded bovine brain samples identified as bovine unspecific encephalitis
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.4172/2157-7579.1000145 http://hdl.handle.net/11449/141083 |
Resumo: | Background: Bovine herpes virus 5 (BoHV-5) is a neurovirulent alpha herpes virus that causes fatal meningoencephalitis and huge economic losses in the livestock and associated industries worldwide; its diagnosis in the laboratory is essential for disease control, immunologic surveillance and effective monitoring programs in endemic areas. Materials and Methods: In this study, brain samples from 21 Colombian cattle that died of neurological disease, were negative for rabies and were diagnosed with nonspecific encephalitis were processed by real-time PCR (qPCR) in the laboratory of virology at the Institute of Biosciences, Sao Paulo State University, Brazil, to detect BoHV-5 genetic material in tissue preserved in paraffin blocks. Tissues were considered positive for BoHV-5 if amplification occurred at or before 30 PCR cycles, inconclusive if amplification occurred between 30 and 40 cycles and negative otherwise. Results and Conclusion: Two cases were positive, demonstrating that qPCR may be useful for confirming herpes encephalitis in acid formalin-fixed tissues with an inconclusive diagnosis. |
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Repositório Institucional da UNESP |
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spelling |
The use of real-time PCR (qPCR) for the diagnosis of bovine herpes virus 5 in formalin-fixed, paraffin-embedded bovine brain samples identified as bovine unspecific encephalitisBovine herpes virus 5Real-time PCRDiagnosisBoHV-5Background: Bovine herpes virus 5 (BoHV-5) is a neurovirulent alpha herpes virus that causes fatal meningoencephalitis and huge economic losses in the livestock and associated industries worldwide; its diagnosis in the laboratory is essential for disease control, immunologic surveillance and effective monitoring programs in endemic areas. Materials and Methods: In this study, brain samples from 21 Colombian cattle that died of neurological disease, were negative for rabies and were diagnosed with nonspecific encephalitis were processed by real-time PCR (qPCR) in the laboratory of virology at the Institute of Biosciences, Sao Paulo State University, Brazil, to detect BoHV-5 genetic material in tissue preserved in paraffin blocks. Tissues were considered positive for BoHV-5 if amplification occurred at or before 30 PCR cycles, inconclusive if amplification occurred between 30 and 40 cycles and negative otherwise. Results and Conclusion: Two cases were positive, demonstrating that qPCR may be useful for confirming herpes encephalitis in acid formalin-fixed tissues with an inconclusive diagnosis.Universidade Estadual Paulista, Departamento de Patologia Veterinária, Faculdade de Ciências Agrárias e Veterinárias de BotucatuUniversidade Estadual Paulista, Departamento de Microbiologia e Imunologia, Instituto de Biociências de BotucatuUniversidade Estadual Paulista, Departamento de Patologia, Faculdade de Medicina de BotucatuUniversidade Estadual Paulista (Unesp)Universidad de CaldasPedraza-Ordoñez, Francisco [UNESP]Yamatogi, Ricardo Seidi [UNESP]Araújo Júnior, João Pessoa [UNESP]Rocha, Noeme Souza [UNESP]Alessi, Antonio Carlos [UNESP]2016-07-07T12:36:46Z2016-07-07T12:36:46Z2013info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1-5application/pdfhttp://dx.doi.org/10.4172/2157-7579.1000145Journal of Veterinary Science & Technology, v. 4, n. 5, p. 1-5, 2013.2157-7579http://hdl.handle.net/11449/14108310.4172/2157-7579.1000145ISSN2157-7579-2013-04-05-01-05.pdf60777359184692840000-0002-8188-8149Currículo Lattesreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Veterinary Science & Technology0,193info:eu-repo/semantics/openAccess2023-11-05T06:07:23Zoai:repositorio.unesp.br:11449/141083Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-11-05T06:07:23Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
The use of real-time PCR (qPCR) for the diagnosis of bovine herpes virus 5 in formalin-fixed, paraffin-embedded bovine brain samples identified as bovine unspecific encephalitis |
title |
The use of real-time PCR (qPCR) for the diagnosis of bovine herpes virus 5 in formalin-fixed, paraffin-embedded bovine brain samples identified as bovine unspecific encephalitis |
spellingShingle |
The use of real-time PCR (qPCR) for the diagnosis of bovine herpes virus 5 in formalin-fixed, paraffin-embedded bovine brain samples identified as bovine unspecific encephalitis Pedraza-Ordoñez, Francisco [UNESP] Bovine herpes virus 5 Real-time PCR Diagnosis BoHV-5 |
title_short |
The use of real-time PCR (qPCR) for the diagnosis of bovine herpes virus 5 in formalin-fixed, paraffin-embedded bovine brain samples identified as bovine unspecific encephalitis |
title_full |
The use of real-time PCR (qPCR) for the diagnosis of bovine herpes virus 5 in formalin-fixed, paraffin-embedded bovine brain samples identified as bovine unspecific encephalitis |
title_fullStr |
The use of real-time PCR (qPCR) for the diagnosis of bovine herpes virus 5 in formalin-fixed, paraffin-embedded bovine brain samples identified as bovine unspecific encephalitis |
title_full_unstemmed |
The use of real-time PCR (qPCR) for the diagnosis of bovine herpes virus 5 in formalin-fixed, paraffin-embedded bovine brain samples identified as bovine unspecific encephalitis |
title_sort |
The use of real-time PCR (qPCR) for the diagnosis of bovine herpes virus 5 in formalin-fixed, paraffin-embedded bovine brain samples identified as bovine unspecific encephalitis |
author |
Pedraza-Ordoñez, Francisco [UNESP] |
author_facet |
Pedraza-Ordoñez, Francisco [UNESP] Yamatogi, Ricardo Seidi [UNESP] Araújo Júnior, João Pessoa [UNESP] Rocha, Noeme Souza [UNESP] Alessi, Antonio Carlos [UNESP] |
author_role |
author |
author2 |
Yamatogi, Ricardo Seidi [UNESP] Araújo Júnior, João Pessoa [UNESP] Rocha, Noeme Souza [UNESP] Alessi, Antonio Carlos [UNESP] |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidad de Caldas |
dc.contributor.author.fl_str_mv |
Pedraza-Ordoñez, Francisco [UNESP] Yamatogi, Ricardo Seidi [UNESP] Araújo Júnior, João Pessoa [UNESP] Rocha, Noeme Souza [UNESP] Alessi, Antonio Carlos [UNESP] |
dc.subject.por.fl_str_mv |
Bovine herpes virus 5 Real-time PCR Diagnosis BoHV-5 |
topic |
Bovine herpes virus 5 Real-time PCR Diagnosis BoHV-5 |
description |
Background: Bovine herpes virus 5 (BoHV-5) is a neurovirulent alpha herpes virus that causes fatal meningoencephalitis and huge economic losses in the livestock and associated industries worldwide; its diagnosis in the laboratory is essential for disease control, immunologic surveillance and effective monitoring programs in endemic areas. Materials and Methods: In this study, brain samples from 21 Colombian cattle that died of neurological disease, were negative for rabies and were diagnosed with nonspecific encephalitis were processed by real-time PCR (qPCR) in the laboratory of virology at the Institute of Biosciences, Sao Paulo State University, Brazil, to detect BoHV-5 genetic material in tissue preserved in paraffin blocks. Tissues were considered positive for BoHV-5 if amplification occurred at or before 30 PCR cycles, inconclusive if amplification occurred between 30 and 40 cycles and negative otherwise. Results and Conclusion: Two cases were positive, demonstrating that qPCR may be useful for confirming herpes encephalitis in acid formalin-fixed tissues with an inconclusive diagnosis. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013 2016-07-07T12:36:46Z 2016-07-07T12:36:46Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.4172/2157-7579.1000145 Journal of Veterinary Science & Technology, v. 4, n. 5, p. 1-5, 2013. 2157-7579 http://hdl.handle.net/11449/141083 10.4172/2157-7579.1000145 ISSN2157-7579-2013-04-05-01-05.pdf 6077735918469284 0000-0002-8188-8149 |
url |
http://dx.doi.org/10.4172/2157-7579.1000145 http://hdl.handle.net/11449/141083 |
identifier_str_mv |
Journal of Veterinary Science & Technology, v. 4, n. 5, p. 1-5, 2013. 2157-7579 10.4172/2157-7579.1000145 ISSN2157-7579-2013-04-05-01-05.pdf 6077735918469284 0000-0002-8188-8149 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Veterinary Science & Technology 0,193 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1-5 application/pdf |
dc.source.none.fl_str_mv |
Currículo Lattes reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799964824546836480 |