Chromosome localization of the ribosomal genes 18S and 5S in four stocks of rainbow trout (Oncorhynchus mykiss) from cultivated and naturalized stocks in Brazil
Autor(a) principal: | |
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Data de Publicação: | 2004 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1508/cytologia.69.175 http://hdl.handle.net/11449/67746 |
Resumo: | In the present study, fluorescence in situ hybridization (FISH) was employed to determine the chromosomal location of genes 18S rDNA and 5S rDNA in four rainbow trout stocks. In specimens from the stocks of Núcleo Experimental de Salmonicultura de Campos do Jordão and Gavião river, 18S genes were located at a subterminal position in the long arms of two submetacentric chromosomes, whereas in specimens from stocks of Mount Shasta and Teresópolis they were found in the short arms. In all analyzed stocks, 5S genes were located in two chromosome pairs. In a subtelocentric pair, 5S genes were present in the short arms and, in the other submetacentric pair, 5S genes were at an interstitial position. In the latter, 18S and 5S genes were contiguous. Taking into account that both 18S and 5S rDNA genes have been localized in the short arm of a submetacentric chromosome in almost all rainbow trout samples so far studied, the presence of such genes in the long arm, as seen in the samples from Núcleo Experimental de Salmonicultura de Campos do Jordão and Gavião river, supports the hypothesis of a pericentric inversion involving this chromosome segment in the ancestor line of these stocks. The observed polymorphism allowed the identification of a very useful genomic marker, and may therefore constitute an important tool in the genetic management of rainbow trout stocks. |
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Chromosome localization of the ribosomal genes 18S and 5S in four stocks of rainbow trout (Oncorhynchus mykiss) from cultivated and naturalized stocks in BrazilFish cytogeneticsRainbow troutrDNA genesOncorhynchusOncorhynchus mykissSalmonidaeIn the present study, fluorescence in situ hybridization (FISH) was employed to determine the chromosomal location of genes 18S rDNA and 5S rDNA in four rainbow trout stocks. In specimens from the stocks of Núcleo Experimental de Salmonicultura de Campos do Jordão and Gavião river, 18S genes were located at a subterminal position in the long arms of two submetacentric chromosomes, whereas in specimens from stocks of Mount Shasta and Teresópolis they were found in the short arms. In all analyzed stocks, 5S genes were located in two chromosome pairs. In a subtelocentric pair, 5S genes were present in the short arms and, in the other submetacentric pair, 5S genes were at an interstitial position. In the latter, 18S and 5S genes were contiguous. Taking into account that both 18S and 5S rDNA genes have been localized in the short arm of a submetacentric chromosome in almost all rainbow trout samples so far studied, the presence of such genes in the long arm, as seen in the samples from Núcleo Experimental de Salmonicultura de Campos do Jordão and Gavião river, supports the hypothesis of a pericentric inversion involving this chromosome segment in the ancestor line of these stocks. The observed polymorphism allowed the identification of a very useful genomic marker, and may therefore constitute an important tool in the genetic management of rainbow trout stocks.Depto. Ciê. Biológicas Faculdade de Ciências Campus de Bauru, 17033-360, Bauru, SPDepartamento de Morfologia Instituto de Biociências Campus de Botucatu, 18618-000, Botucatu, SPEstacao Exp. de Salmonicultura Instituto de Pesca, 12460-000, Campos do Jordao, SPUniversidade Estadual Paulista (Unesp)Instituto de PescaPorto-Foresti, FábioOliveira, ClaudioTabata, Yara AikoRigolino, Marcos GuilhermeForesti, Fausto2014-05-27T11:21:05Z2014-05-27T11:21:05Z2004-06-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article175-179application/pdfhttp://dx.doi.org/10.1508/cytologia.69.175Cytologia, v. 69, n. 2, p. 175-179, 2004.0011-4545http://hdl.handle.net/11449/6774610.1508/cytologia.69.1752-s2.0-45443266352-s2.0-4544326635.pdf08047939448463670804793944846367Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengCytologia0.4810,233info:eu-repo/semantics/openAccess2023-11-25T06:12:19Zoai:repositorio.unesp.br:11449/67746Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T18:39:21.217857Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Chromosome localization of the ribosomal genes 18S and 5S in four stocks of rainbow trout (Oncorhynchus mykiss) from cultivated and naturalized stocks in Brazil |
title |
Chromosome localization of the ribosomal genes 18S and 5S in four stocks of rainbow trout (Oncorhynchus mykiss) from cultivated and naturalized stocks in Brazil |
spellingShingle |
Chromosome localization of the ribosomal genes 18S and 5S in four stocks of rainbow trout (Oncorhynchus mykiss) from cultivated and naturalized stocks in Brazil Porto-Foresti, Fábio Fish cytogenetics Rainbow trout rDNA genes Oncorhynchus Oncorhynchus mykiss Salmonidae |
title_short |
Chromosome localization of the ribosomal genes 18S and 5S in four stocks of rainbow trout (Oncorhynchus mykiss) from cultivated and naturalized stocks in Brazil |
title_full |
Chromosome localization of the ribosomal genes 18S and 5S in four stocks of rainbow trout (Oncorhynchus mykiss) from cultivated and naturalized stocks in Brazil |
title_fullStr |
Chromosome localization of the ribosomal genes 18S and 5S in four stocks of rainbow trout (Oncorhynchus mykiss) from cultivated and naturalized stocks in Brazil |
title_full_unstemmed |
Chromosome localization of the ribosomal genes 18S and 5S in four stocks of rainbow trout (Oncorhynchus mykiss) from cultivated and naturalized stocks in Brazil |
title_sort |
Chromosome localization of the ribosomal genes 18S and 5S in four stocks of rainbow trout (Oncorhynchus mykiss) from cultivated and naturalized stocks in Brazil |
author |
Porto-Foresti, Fábio |
author_facet |
Porto-Foresti, Fábio Oliveira, Claudio Tabata, Yara Aiko Rigolino, Marcos Guilherme Foresti, Fausto |
author_role |
author |
author2 |
Oliveira, Claudio Tabata, Yara Aiko Rigolino, Marcos Guilherme Foresti, Fausto |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Instituto de Pesca |
dc.contributor.author.fl_str_mv |
Porto-Foresti, Fábio Oliveira, Claudio Tabata, Yara Aiko Rigolino, Marcos Guilherme Foresti, Fausto |
dc.subject.por.fl_str_mv |
Fish cytogenetics Rainbow trout rDNA genes Oncorhynchus Oncorhynchus mykiss Salmonidae |
topic |
Fish cytogenetics Rainbow trout rDNA genes Oncorhynchus Oncorhynchus mykiss Salmonidae |
description |
In the present study, fluorescence in situ hybridization (FISH) was employed to determine the chromosomal location of genes 18S rDNA and 5S rDNA in four rainbow trout stocks. In specimens from the stocks of Núcleo Experimental de Salmonicultura de Campos do Jordão and Gavião river, 18S genes were located at a subterminal position in the long arms of two submetacentric chromosomes, whereas in specimens from stocks of Mount Shasta and Teresópolis they were found in the short arms. In all analyzed stocks, 5S genes were located in two chromosome pairs. In a subtelocentric pair, 5S genes were present in the short arms and, in the other submetacentric pair, 5S genes were at an interstitial position. In the latter, 18S and 5S genes were contiguous. Taking into account that both 18S and 5S rDNA genes have been localized in the short arm of a submetacentric chromosome in almost all rainbow trout samples so far studied, the presence of such genes in the long arm, as seen in the samples from Núcleo Experimental de Salmonicultura de Campos do Jordão and Gavião river, supports the hypothesis of a pericentric inversion involving this chromosome segment in the ancestor line of these stocks. The observed polymorphism allowed the identification of a very useful genomic marker, and may therefore constitute an important tool in the genetic management of rainbow trout stocks. |
publishDate |
2004 |
dc.date.none.fl_str_mv |
2004-06-01 2014-05-27T11:21:05Z 2014-05-27T11:21:05Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1508/cytologia.69.175 Cytologia, v. 69, n. 2, p. 175-179, 2004. 0011-4545 http://hdl.handle.net/11449/67746 10.1508/cytologia.69.175 2-s2.0-4544326635 2-s2.0-4544326635.pdf 0804793944846367 0804793944846367 |
url |
http://dx.doi.org/10.1508/cytologia.69.175 http://hdl.handle.net/11449/67746 |
identifier_str_mv |
Cytologia, v. 69, n. 2, p. 175-179, 2004. 0011-4545 10.1508/cytologia.69.175 2-s2.0-4544326635 2-s2.0-4544326635.pdf 0804793944846367 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Cytologia 0.481 0,233 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
175-179 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1808128228629413888 |