Chromosome localization of the ribosomal genes 18S and 5S in four stocks of rainbow trout (Oncorhynchus mykiss) from cultivated and naturalized stocks in Brazil

Detalhes bibliográficos
Autor(a) principal: Porto-Foresti, Fábio
Data de Publicação: 2004
Outros Autores: Oliveira, Claudio, Tabata, Yara Aiko, Rigolino, Marcos Guilherme, Foresti, Fausto
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1508/cytologia.69.175
http://hdl.handle.net/11449/67746
Resumo: In the present study, fluorescence in situ hybridization (FISH) was employed to determine the chromosomal location of genes 18S rDNA and 5S rDNA in four rainbow trout stocks. In specimens from the stocks of Núcleo Experimental de Salmonicultura de Campos do Jordão and Gavião river, 18S genes were located at a subterminal position in the long arms of two submetacentric chromosomes, whereas in specimens from stocks of Mount Shasta and Teresópolis they were found in the short arms. In all analyzed stocks, 5S genes were located in two chromosome pairs. In a subtelocentric pair, 5S genes were present in the short arms and, in the other submetacentric pair, 5S genes were at an interstitial position. In the latter, 18S and 5S genes were contiguous. Taking into account that both 18S and 5S rDNA genes have been localized in the short arm of a submetacentric chromosome in almost all rainbow trout samples so far studied, the presence of such genes in the long arm, as seen in the samples from Núcleo Experimental de Salmonicultura de Campos do Jordão and Gavião river, supports the hypothesis of a pericentric inversion involving this chromosome segment in the ancestor line of these stocks. The observed polymorphism allowed the identification of a very useful genomic marker, and may therefore constitute an important tool in the genetic management of rainbow trout stocks.
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spelling Chromosome localization of the ribosomal genes 18S and 5S in four stocks of rainbow trout (Oncorhynchus mykiss) from cultivated and naturalized stocks in BrazilFish cytogeneticsRainbow troutrDNA genesOncorhynchusOncorhynchus mykissSalmonidaeIn the present study, fluorescence in situ hybridization (FISH) was employed to determine the chromosomal location of genes 18S rDNA and 5S rDNA in four rainbow trout stocks. In specimens from the stocks of Núcleo Experimental de Salmonicultura de Campos do Jordão and Gavião river, 18S genes were located at a subterminal position in the long arms of two submetacentric chromosomes, whereas in specimens from stocks of Mount Shasta and Teresópolis they were found in the short arms. In all analyzed stocks, 5S genes were located in two chromosome pairs. In a subtelocentric pair, 5S genes were present in the short arms and, in the other submetacentric pair, 5S genes were at an interstitial position. In the latter, 18S and 5S genes were contiguous. Taking into account that both 18S and 5S rDNA genes have been localized in the short arm of a submetacentric chromosome in almost all rainbow trout samples so far studied, the presence of such genes in the long arm, as seen in the samples from Núcleo Experimental de Salmonicultura de Campos do Jordão and Gavião river, supports the hypothesis of a pericentric inversion involving this chromosome segment in the ancestor line of these stocks. The observed polymorphism allowed the identification of a very useful genomic marker, and may therefore constitute an important tool in the genetic management of rainbow trout stocks.Depto. Ciê. Biológicas Faculdade de Ciências Campus de Bauru, 17033-360, Bauru, SPDepartamento de Morfologia Instituto de Biociências Campus de Botucatu, 18618-000, Botucatu, SPEstacao Exp. de Salmonicultura Instituto de Pesca, 12460-000, Campos do Jordao, SPUniversidade Estadual Paulista (Unesp)Instituto de PescaPorto-Foresti, FábioOliveira, ClaudioTabata, Yara AikoRigolino, Marcos GuilhermeForesti, Fausto2014-05-27T11:21:05Z2014-05-27T11:21:05Z2004-06-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article175-179application/pdfhttp://dx.doi.org/10.1508/cytologia.69.175Cytologia, v. 69, n. 2, p. 175-179, 2004.0011-4545http://hdl.handle.net/11449/6774610.1508/cytologia.69.1752-s2.0-45443266352-s2.0-4544326635.pdf08047939448463670804793944846367Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengCytologia0.4810,233info:eu-repo/semantics/openAccess2023-11-25T06:12:19Zoai:repositorio.unesp.br:11449/67746Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T18:39:21.217857Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Chromosome localization of the ribosomal genes 18S and 5S in four stocks of rainbow trout (Oncorhynchus mykiss) from cultivated and naturalized stocks in Brazil
title Chromosome localization of the ribosomal genes 18S and 5S in four stocks of rainbow trout (Oncorhynchus mykiss) from cultivated and naturalized stocks in Brazil
spellingShingle Chromosome localization of the ribosomal genes 18S and 5S in four stocks of rainbow trout (Oncorhynchus mykiss) from cultivated and naturalized stocks in Brazil
Porto-Foresti, Fábio
Fish cytogenetics
Rainbow trout
rDNA genes
Oncorhynchus
Oncorhynchus mykiss
Salmonidae
title_short Chromosome localization of the ribosomal genes 18S and 5S in four stocks of rainbow trout (Oncorhynchus mykiss) from cultivated and naturalized stocks in Brazil
title_full Chromosome localization of the ribosomal genes 18S and 5S in four stocks of rainbow trout (Oncorhynchus mykiss) from cultivated and naturalized stocks in Brazil
title_fullStr Chromosome localization of the ribosomal genes 18S and 5S in four stocks of rainbow trout (Oncorhynchus mykiss) from cultivated and naturalized stocks in Brazil
title_full_unstemmed Chromosome localization of the ribosomal genes 18S and 5S in four stocks of rainbow trout (Oncorhynchus mykiss) from cultivated and naturalized stocks in Brazil
title_sort Chromosome localization of the ribosomal genes 18S and 5S in four stocks of rainbow trout (Oncorhynchus mykiss) from cultivated and naturalized stocks in Brazil
author Porto-Foresti, Fábio
author_facet Porto-Foresti, Fábio
Oliveira, Claudio
Tabata, Yara Aiko
Rigolino, Marcos Guilherme
Foresti, Fausto
author_role author
author2 Oliveira, Claudio
Tabata, Yara Aiko
Rigolino, Marcos Guilherme
Foresti, Fausto
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Instituto de Pesca
dc.contributor.author.fl_str_mv Porto-Foresti, Fábio
Oliveira, Claudio
Tabata, Yara Aiko
Rigolino, Marcos Guilherme
Foresti, Fausto
dc.subject.por.fl_str_mv Fish cytogenetics
Rainbow trout
rDNA genes
Oncorhynchus
Oncorhynchus mykiss
Salmonidae
topic Fish cytogenetics
Rainbow trout
rDNA genes
Oncorhynchus
Oncorhynchus mykiss
Salmonidae
description In the present study, fluorescence in situ hybridization (FISH) was employed to determine the chromosomal location of genes 18S rDNA and 5S rDNA in four rainbow trout stocks. In specimens from the stocks of Núcleo Experimental de Salmonicultura de Campos do Jordão and Gavião river, 18S genes were located at a subterminal position in the long arms of two submetacentric chromosomes, whereas in specimens from stocks of Mount Shasta and Teresópolis they were found in the short arms. In all analyzed stocks, 5S genes were located in two chromosome pairs. In a subtelocentric pair, 5S genes were present in the short arms and, in the other submetacentric pair, 5S genes were at an interstitial position. In the latter, 18S and 5S genes were contiguous. Taking into account that both 18S and 5S rDNA genes have been localized in the short arm of a submetacentric chromosome in almost all rainbow trout samples so far studied, the presence of such genes in the long arm, as seen in the samples from Núcleo Experimental de Salmonicultura de Campos do Jordão and Gavião river, supports the hypothesis of a pericentric inversion involving this chromosome segment in the ancestor line of these stocks. The observed polymorphism allowed the identification of a very useful genomic marker, and may therefore constitute an important tool in the genetic management of rainbow trout stocks.
publishDate 2004
dc.date.none.fl_str_mv 2004-06-01
2014-05-27T11:21:05Z
2014-05-27T11:21:05Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1508/cytologia.69.175
Cytologia, v. 69, n. 2, p. 175-179, 2004.
0011-4545
http://hdl.handle.net/11449/67746
10.1508/cytologia.69.175
2-s2.0-4544326635
2-s2.0-4544326635.pdf
0804793944846367
0804793944846367
url http://dx.doi.org/10.1508/cytologia.69.175
http://hdl.handle.net/11449/67746
identifier_str_mv Cytologia, v. 69, n. 2, p. 175-179, 2004.
0011-4545
10.1508/cytologia.69.175
2-s2.0-4544326635
2-s2.0-4544326635.pdf
0804793944846367
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Cytologia
0.481
0,233
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 175-179
application/pdf
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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