Inorganic elemental analysis and identification of residual monomers released from different glass ionomer cements in cell culture medium
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/S1807-25772013000400007 http://hdl.handle.net/11449/122923 |
Resumo: | INTRODUCTION: Glass ionomer cements (GICs) release inorganic elements and organic residual monomers with the potential for deleterious effects on pulp cells. OBJECTIVE: To identify and quantify inorganic elements present in different GICs and released components from these materials in cell culture medium. MATERIAL AND METHOD: Samples of two resin-modified GICs for base/liner (Vitrebond and Fuji Lining LC), two resin-modified restorative GICs (Vitremer and Fuji II LC) and two conventional restorative GICs (Ketac Fil Plus and Ketac Molar Easymix) were prepared and analyzed by Energy-Dispersive X-Ray Fluorescence Spectrometry (EDXRF). Extracts of these materials were obtained by immersion of each sample in separate containers of DMEM for 24 h (total surface-liquid ratio = 45.7 mm²/mL). The extracts were analyzed by EDXRF and Gas Chromatography-Mass Spectrometry (GC-MS). RESULT: Higher percentages of strontium, silicon and aluminum were identified in Vitrebond, Vitremer, Fuji Lining LC, Fuji II LC, and Ketac Fil Plus, while zinc was detected only in Vitrebond. Ketac Molar Easymix presented a greater atomic composition of lanthanum, calcium, aluminum and silicon. Strontium was detected in the extracts from all materials except Ketac Molar Easymix; calcium was present in extracts from Ketac Fil Plus; zinc only in Vitrebond; and silicon in Fuji II LC extract. The analysis by GC-MS detected 2-hydroxyethyl-methacrylate (HEMA) in the extracts from all resin-modified GICs, and iodine benzene was detected only in the Vitrebond extract. CONCLUSION: Of the GICs sampled, Vitrebond released the highest number of components with cytotoxic potential. |
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Inorganic elemental analysis and identification of residual monomers released from different glass ionomer cements in cell culture mediumAnálise elementar inorgânica e identificação de monômeros residuais orgânicos liberados por diferentes cimentos de ionômero de vidroCimentos de ionômeros de vidrocompostos inorgânicosGlass ionomer cementsinorganic chemicalsINTRODUCTION: Glass ionomer cements (GICs) release inorganic elements and organic residual monomers with the potential for deleterious effects on pulp cells. OBJECTIVE: To identify and quantify inorganic elements present in different GICs and released components from these materials in cell culture medium. MATERIAL AND METHOD: Samples of two resin-modified GICs for base/liner (Vitrebond and Fuji Lining LC), two resin-modified restorative GICs (Vitremer and Fuji II LC) and two conventional restorative GICs (Ketac Fil Plus and Ketac Molar Easymix) were prepared and analyzed by Energy-Dispersive X-Ray Fluorescence Spectrometry (EDXRF). Extracts of these materials were obtained by immersion of each sample in separate containers of DMEM for 24 h (total surface-liquid ratio = 45.7 mm²/mL). The extracts were analyzed by EDXRF and Gas Chromatography-Mass Spectrometry (GC-MS). RESULT: Higher percentages of strontium, silicon and aluminum were identified in Vitrebond, Vitremer, Fuji Lining LC, Fuji II LC, and Ketac Fil Plus, while zinc was detected only in Vitrebond. Ketac Molar Easymix presented a greater atomic composition of lanthanum, calcium, aluminum and silicon. Strontium was detected in the extracts from all materials except Ketac Molar Easymix; calcium was present in extracts from Ketac Fil Plus; zinc only in Vitrebond; and silicon in Fuji II LC extract. The analysis by GC-MS detected 2-hydroxyethyl-methacrylate (HEMA) in the extracts from all resin-modified GICs, and iodine benzene was detected only in the Vitrebond extract. CONCLUSION: Of the GICs sampled, Vitrebond released the highest number of components with cytotoxic potential.INTRODUÇÃO: Os cimentos de ionômero de vidro (CIVs) liberam elementos inorgânicos e monômeros orgânicos residuais que têm o potencial de causar efeitos deletérios sobre as células pulpares. OBJETIVO: Identificar e quantificar os elementos inorgânicos presentes em diferentes CIVs, bem como os componentes liberados por estes materiais em meio de cultura celular. MATERIAL E MÉTODO: Espécimes cilindricos de dois CIVs modificados por resina para base/forramento (Vitrebond e Fuji Lining LC), dois CIVs modificados por resina restauradores (Vitremer e Fuji II LC) e dois CIVs convencionais restauradores (Ketac Fil Plus e Ketac Molar Easymix) foram preparados e analisados por Espectrometria de Fluorescência de Raios X por Energia Dispersiva (EDXRF). Em seguida, extratos de 24h desses materiais foram obtidos e analisados por EDXRF e por Cromatografia Gasosa/Espectrometria de Massa (CG/EM). RESULTADO: Os elementos inorgânicos identificados em maior porcentagem nos CIVs Vitrebond, Fuji Lining LC, Vitremer, Fuji II LC e Ketac Fil Plus foram estrôncio, silício e alumínio, enquanto o zinco foi detectado apenas no Vitrebond. O Ketac Molar Easymix apresentou maior porcentagem dos elementos lantânio, cálcio, alumínio e silício. Estrôncio foi detectado nos extratos de todos os materiais, exceto no Ketac Molar Easymix; cálcio estava presente no extrato do Ketac Fil Plus; zinco apenas no Vitrebond; e silício no extrato do Fuji II LC . O HEMA foi identificado nos extratos de todos os CIVs modificados por resina, e o iodobenzeno, somente no Vitrebond. CONCLUSÃO: Entre os CIVs estudados, o Vitrebond é o que libera mais componentes com potencial citotóxico.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Univ Estadual Paulista Faculdade de OdontologiaUniv Estadual Paulista Instituto de QuímicaUniv Estadual Paulista Faculdade de OdontologiaUniv Estadual Paulista Instituto de QuímicaCNPq: 306029/2004-9Universidade Estadual Paulista Júlio de Mesquita FilhoUniversidade Estadual Paulista (Unesp)Tanaka, Marcia Hiromi [UNESP]Alécio, Alberto Camilo [UNESP]Flumignan, Danilo Luiz [UNESP]Oliveira, José Eduardo de [UNESP]Giro, Elisa Maria Aparecida [UNESP]2015-05-13T16:35:42Z2015-05-13T16:35:42Z2013-08-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article273-282application/pdfhttp://dx.doi.org/10.1590/S1807-25772013000400007Revista de Odontologia da UNESP. Universidade Estadual Paulista Júlio de Mesquita Filho, v. 42, n. 4, p. 273-282, 2013.1807-2577http://hdl.handle.net/11449/12292310.1590/S1807-25772013000400007S1807-25772013000400007S1807-25772013000400007.pdfSciELOreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengRevista de Odontologia da UNESPinfo:eu-repo/semantics/openAccess2023-10-15T06:07:02Zoai:repositorio.unesp.br:11449/122923Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T15:01:10.007650Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Inorganic elemental analysis and identification of residual monomers released from different glass ionomer cements in cell culture medium Análise elementar inorgânica e identificação de monômeros residuais orgânicos liberados por diferentes cimentos de ionômero de vidro |
title |
Inorganic elemental analysis and identification of residual monomers released from different glass ionomer cements in cell culture medium |
spellingShingle |
Inorganic elemental analysis and identification of residual monomers released from different glass ionomer cements in cell culture medium Tanaka, Marcia Hiromi [UNESP] Cimentos de ionômeros de vidro compostos inorgânicos Glass ionomer cements inorganic chemicals |
title_short |
Inorganic elemental analysis and identification of residual monomers released from different glass ionomer cements in cell culture medium |
title_full |
Inorganic elemental analysis and identification of residual monomers released from different glass ionomer cements in cell culture medium |
title_fullStr |
Inorganic elemental analysis and identification of residual monomers released from different glass ionomer cements in cell culture medium |
title_full_unstemmed |
Inorganic elemental analysis and identification of residual monomers released from different glass ionomer cements in cell culture medium |
title_sort |
Inorganic elemental analysis and identification of residual monomers released from different glass ionomer cements in cell culture medium |
author |
Tanaka, Marcia Hiromi [UNESP] |
author_facet |
Tanaka, Marcia Hiromi [UNESP] Alécio, Alberto Camilo [UNESP] Flumignan, Danilo Luiz [UNESP] Oliveira, José Eduardo de [UNESP] Giro, Elisa Maria Aparecida [UNESP] |
author_role |
author |
author2 |
Alécio, Alberto Camilo [UNESP] Flumignan, Danilo Luiz [UNESP] Oliveira, José Eduardo de [UNESP] Giro, Elisa Maria Aparecida [UNESP] |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Tanaka, Marcia Hiromi [UNESP] Alécio, Alberto Camilo [UNESP] Flumignan, Danilo Luiz [UNESP] Oliveira, José Eduardo de [UNESP] Giro, Elisa Maria Aparecida [UNESP] |
dc.subject.por.fl_str_mv |
Cimentos de ionômeros de vidro compostos inorgânicos Glass ionomer cements inorganic chemicals |
topic |
Cimentos de ionômeros de vidro compostos inorgânicos Glass ionomer cements inorganic chemicals |
description |
INTRODUCTION: Glass ionomer cements (GICs) release inorganic elements and organic residual monomers with the potential for deleterious effects on pulp cells. OBJECTIVE: To identify and quantify inorganic elements present in different GICs and released components from these materials in cell culture medium. MATERIAL AND METHOD: Samples of two resin-modified GICs for base/liner (Vitrebond and Fuji Lining LC), two resin-modified restorative GICs (Vitremer and Fuji II LC) and two conventional restorative GICs (Ketac Fil Plus and Ketac Molar Easymix) were prepared and analyzed by Energy-Dispersive X-Ray Fluorescence Spectrometry (EDXRF). Extracts of these materials were obtained by immersion of each sample in separate containers of DMEM for 24 h (total surface-liquid ratio = 45.7 mm²/mL). The extracts were analyzed by EDXRF and Gas Chromatography-Mass Spectrometry (GC-MS). RESULT: Higher percentages of strontium, silicon and aluminum were identified in Vitrebond, Vitremer, Fuji Lining LC, Fuji II LC, and Ketac Fil Plus, while zinc was detected only in Vitrebond. Ketac Molar Easymix presented a greater atomic composition of lanthanum, calcium, aluminum and silicon. Strontium was detected in the extracts from all materials except Ketac Molar Easymix; calcium was present in extracts from Ketac Fil Plus; zinc only in Vitrebond; and silicon in Fuji II LC extract. The analysis by GC-MS detected 2-hydroxyethyl-methacrylate (HEMA) in the extracts from all resin-modified GICs, and iodine benzene was detected only in the Vitrebond extract. CONCLUSION: Of the GICs sampled, Vitrebond released the highest number of components with cytotoxic potential. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-08-01 2015-05-13T16:35:42Z 2015-05-13T16:35:42Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/S1807-25772013000400007 Revista de Odontologia da UNESP. Universidade Estadual Paulista Júlio de Mesquita Filho, v. 42, n. 4, p. 273-282, 2013. 1807-2577 http://hdl.handle.net/11449/122923 10.1590/S1807-25772013000400007 S1807-25772013000400007 S1807-25772013000400007.pdf |
url |
http://dx.doi.org/10.1590/S1807-25772013000400007 http://hdl.handle.net/11449/122923 |
identifier_str_mv |
Revista de Odontologia da UNESP. Universidade Estadual Paulista Júlio de Mesquita Filho, v. 42, n. 4, p. 273-282, 2013. 1807-2577 10.1590/S1807-25772013000400007 S1807-25772013000400007 S1807-25772013000400007.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Revista de Odontologia da UNESP |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
273-282 application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Estadual Paulista Júlio de Mesquita Filho |
publisher.none.fl_str_mv |
Universidade Estadual Paulista Júlio de Mesquita Filho |
dc.source.none.fl_str_mv |
SciELO reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1808128446680793088 |