Inorganic elemental analysis and identification of residual monomers released from different glass ionomer cements in cell culture medium

Detalhes bibliográficos
Autor(a) principal: Tanaka, Marcia Hiromi [UNESP]
Data de Publicação: 2013
Outros Autores: Alécio, Alberto Camilo [UNESP], Flumignan, Danilo Luiz [UNESP], Oliveira, José Eduardo de [UNESP], Giro, Elisa Maria Aparecida [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1590/S1807-25772013000400007
http://hdl.handle.net/11449/122923
Resumo: INTRODUCTION: Glass ionomer cements (GICs) release inorganic elements and organic residual monomers with the potential for deleterious effects on pulp cells. OBJECTIVE: To identify and quantify inorganic elements present in different GICs and released components from these materials in cell culture medium. MATERIAL AND METHOD: Samples of two resin-modified GICs for base/liner (Vitrebond and Fuji Lining LC), two resin-modified restorative GICs (Vitremer and Fuji II LC) and two conventional restorative GICs (Ketac Fil Plus and Ketac Molar Easymix) were prepared and analyzed by Energy-Dispersive X-Ray Fluorescence Spectrometry (EDXRF). Extracts of these materials were obtained by immersion of each sample in separate containers of DMEM for 24 h (total surface-liquid ratio = 45.7 mm²/mL). The extracts were analyzed by EDXRF and Gas Chromatography-Mass Spectrometry (GC-MS). RESULT: Higher percentages of strontium, silicon and aluminum were identified in Vitrebond, Vitremer, Fuji Lining LC, Fuji II LC, and Ketac Fil Plus, while zinc was detected only in Vitrebond. Ketac Molar Easymix presented a greater atomic composition of lanthanum, calcium, aluminum and silicon. Strontium was detected in the extracts from all materials except Ketac Molar Easymix; calcium was present in extracts from Ketac Fil Plus; zinc only in Vitrebond; and silicon in Fuji II LC extract. The analysis by GC-MS detected 2-hydroxyethyl-methacrylate (HEMA) in the extracts from all resin-modified GICs, and iodine benzene was detected only in the Vitrebond extract. CONCLUSION: Of the GICs sampled, Vitrebond released the highest number of components with cytotoxic potential.
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spelling Inorganic elemental analysis and identification of residual monomers released from different glass ionomer cements in cell culture mediumAnálise elementar inorgânica e identificação de monômeros residuais orgânicos liberados por diferentes cimentos de ionômero de vidroCimentos de ionômeros de vidrocompostos inorgânicosGlass ionomer cementsinorganic chemicalsINTRODUCTION: Glass ionomer cements (GICs) release inorganic elements and organic residual monomers with the potential for deleterious effects on pulp cells. OBJECTIVE: To identify and quantify inorganic elements present in different GICs and released components from these materials in cell culture medium. MATERIAL AND METHOD: Samples of two resin-modified GICs for base/liner (Vitrebond and Fuji Lining LC), two resin-modified restorative GICs (Vitremer and Fuji II LC) and two conventional restorative GICs (Ketac Fil Plus and Ketac Molar Easymix) were prepared and analyzed by Energy-Dispersive X-Ray Fluorescence Spectrometry (EDXRF). Extracts of these materials were obtained by immersion of each sample in separate containers of DMEM for 24 h (total surface-liquid ratio = 45.7 mm²/mL). The extracts were analyzed by EDXRF and Gas Chromatography-Mass Spectrometry (GC-MS). RESULT: Higher percentages of strontium, silicon and aluminum were identified in Vitrebond, Vitremer, Fuji Lining LC, Fuji II LC, and Ketac Fil Plus, while zinc was detected only in Vitrebond. Ketac Molar Easymix presented a greater atomic composition of lanthanum, calcium, aluminum and silicon. Strontium was detected in the extracts from all materials except Ketac Molar Easymix; calcium was present in extracts from Ketac Fil Plus; zinc only in Vitrebond; and silicon in Fuji II LC extract. The analysis by GC-MS detected 2-hydroxyethyl-methacrylate (HEMA) in the extracts from all resin-modified GICs, and iodine benzene was detected only in the Vitrebond extract. CONCLUSION: Of the GICs sampled, Vitrebond released the highest number of components with cytotoxic potential.INTRODUÇÃO: Os cimentos de ionômero de vidro (CIVs) liberam elementos inorgânicos e monômeros orgânicos residuais que têm o potencial de causar efeitos deletérios sobre as células pulpares. OBJETIVO: Identificar e quantificar os elementos inorgânicos presentes em diferentes CIVs, bem como os componentes liberados por estes materiais em meio de cultura celular. MATERIAL E MÉTODO: Espécimes cilindricos de dois CIVs modificados por resina para base/forramento (Vitrebond e Fuji Lining LC), dois CIVs modificados por resina restauradores (Vitremer e Fuji II LC) e dois CIVs convencionais restauradores (Ketac Fil Plus e Ketac Molar Easymix) foram preparados e analisados por Espectrometria de Fluorescência de Raios X por Energia Dispersiva (EDXRF). Em seguida, extratos de 24h desses materiais foram obtidos e analisados por EDXRF e por Cromatografia Gasosa/Espectrometria de Massa (CG/EM). RESULTADO: Os elementos inorgânicos identificados em maior porcentagem nos CIVs Vitrebond, Fuji Lining LC, Vitremer, Fuji II LC e Ketac Fil Plus foram estrôncio, silício e alumínio, enquanto o zinco foi detectado apenas no Vitrebond. O Ketac Molar Easymix apresentou maior porcentagem dos elementos lantânio, cálcio, alumínio e silício. Estrôncio foi detectado nos extratos de todos os materiais, exceto no Ketac Molar Easymix; cálcio estava presente no extrato do Ketac Fil Plus; zinco apenas no Vitrebond; e silício no extrato do Fuji II LC . O HEMA foi identificado nos extratos de todos os CIVs modificados por resina, e o iodobenzeno, somente no Vitrebond. CONCLUSÃO: Entre os CIVs estudados, o Vitrebond é o que libera mais componentes com potencial citotóxico.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Univ Estadual Paulista Faculdade de OdontologiaUniv Estadual Paulista Instituto de QuímicaUniv Estadual Paulista Faculdade de OdontologiaUniv Estadual Paulista Instituto de QuímicaCNPq: 306029/2004-9Universidade Estadual Paulista Júlio de Mesquita FilhoUniversidade Estadual Paulista (Unesp)Tanaka, Marcia Hiromi [UNESP]Alécio, Alberto Camilo [UNESP]Flumignan, Danilo Luiz [UNESP]Oliveira, José Eduardo de [UNESP]Giro, Elisa Maria Aparecida [UNESP]2015-05-13T16:35:42Z2015-05-13T16:35:42Z2013-08-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article273-282application/pdfhttp://dx.doi.org/10.1590/S1807-25772013000400007Revista de Odontologia da UNESP. Universidade Estadual Paulista Júlio de Mesquita Filho, v. 42, n. 4, p. 273-282, 2013.1807-2577http://hdl.handle.net/11449/12292310.1590/S1807-25772013000400007S1807-25772013000400007S1807-25772013000400007.pdfSciELOreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengRevista de Odontologia da UNESPinfo:eu-repo/semantics/openAccess2023-10-15T06:07:02Zoai:repositorio.unesp.br:11449/122923Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T15:01:10.007650Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Inorganic elemental analysis and identification of residual monomers released from different glass ionomer cements in cell culture medium
Análise elementar inorgânica e identificação de monômeros residuais orgânicos liberados por diferentes cimentos de ionômero de vidro
title Inorganic elemental analysis and identification of residual monomers released from different glass ionomer cements in cell culture medium
spellingShingle Inorganic elemental analysis and identification of residual monomers released from different glass ionomer cements in cell culture medium
Tanaka, Marcia Hiromi [UNESP]
Cimentos de ionômeros de vidro
compostos inorgânicos
Glass ionomer cements
inorganic chemicals
title_short Inorganic elemental analysis and identification of residual monomers released from different glass ionomer cements in cell culture medium
title_full Inorganic elemental analysis and identification of residual monomers released from different glass ionomer cements in cell culture medium
title_fullStr Inorganic elemental analysis and identification of residual monomers released from different glass ionomer cements in cell culture medium
title_full_unstemmed Inorganic elemental analysis and identification of residual monomers released from different glass ionomer cements in cell culture medium
title_sort Inorganic elemental analysis and identification of residual monomers released from different glass ionomer cements in cell culture medium
author Tanaka, Marcia Hiromi [UNESP]
author_facet Tanaka, Marcia Hiromi [UNESP]
Alécio, Alberto Camilo [UNESP]
Flumignan, Danilo Luiz [UNESP]
Oliveira, José Eduardo de [UNESP]
Giro, Elisa Maria Aparecida [UNESP]
author_role author
author2 Alécio, Alberto Camilo [UNESP]
Flumignan, Danilo Luiz [UNESP]
Oliveira, José Eduardo de [UNESP]
Giro, Elisa Maria Aparecida [UNESP]
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Tanaka, Marcia Hiromi [UNESP]
Alécio, Alberto Camilo [UNESP]
Flumignan, Danilo Luiz [UNESP]
Oliveira, José Eduardo de [UNESP]
Giro, Elisa Maria Aparecida [UNESP]
dc.subject.por.fl_str_mv Cimentos de ionômeros de vidro
compostos inorgânicos
Glass ionomer cements
inorganic chemicals
topic Cimentos de ionômeros de vidro
compostos inorgânicos
Glass ionomer cements
inorganic chemicals
description INTRODUCTION: Glass ionomer cements (GICs) release inorganic elements and organic residual monomers with the potential for deleterious effects on pulp cells. OBJECTIVE: To identify and quantify inorganic elements present in different GICs and released components from these materials in cell culture medium. MATERIAL AND METHOD: Samples of two resin-modified GICs for base/liner (Vitrebond and Fuji Lining LC), two resin-modified restorative GICs (Vitremer and Fuji II LC) and two conventional restorative GICs (Ketac Fil Plus and Ketac Molar Easymix) were prepared and analyzed by Energy-Dispersive X-Ray Fluorescence Spectrometry (EDXRF). Extracts of these materials were obtained by immersion of each sample in separate containers of DMEM for 24 h (total surface-liquid ratio = 45.7 mm²/mL). The extracts were analyzed by EDXRF and Gas Chromatography-Mass Spectrometry (GC-MS). RESULT: Higher percentages of strontium, silicon and aluminum were identified in Vitrebond, Vitremer, Fuji Lining LC, Fuji II LC, and Ketac Fil Plus, while zinc was detected only in Vitrebond. Ketac Molar Easymix presented a greater atomic composition of lanthanum, calcium, aluminum and silicon. Strontium was detected in the extracts from all materials except Ketac Molar Easymix; calcium was present in extracts from Ketac Fil Plus; zinc only in Vitrebond; and silicon in Fuji II LC extract. The analysis by GC-MS detected 2-hydroxyethyl-methacrylate (HEMA) in the extracts from all resin-modified GICs, and iodine benzene was detected only in the Vitrebond extract. CONCLUSION: Of the GICs sampled, Vitrebond released the highest number of components with cytotoxic potential.
publishDate 2013
dc.date.none.fl_str_mv 2013-08-01
2015-05-13T16:35:42Z
2015-05-13T16:35:42Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/S1807-25772013000400007
Revista de Odontologia da UNESP. Universidade Estadual Paulista Júlio de Mesquita Filho, v. 42, n. 4, p. 273-282, 2013.
1807-2577
http://hdl.handle.net/11449/122923
10.1590/S1807-25772013000400007
S1807-25772013000400007
S1807-25772013000400007.pdf
url http://dx.doi.org/10.1590/S1807-25772013000400007
http://hdl.handle.net/11449/122923
identifier_str_mv Revista de Odontologia da UNESP. Universidade Estadual Paulista Júlio de Mesquita Filho, v. 42, n. 4, p. 273-282, 2013.
1807-2577
10.1590/S1807-25772013000400007
S1807-25772013000400007
S1807-25772013000400007.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Revista de Odontologia da UNESP
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 273-282
application/pdf
dc.publisher.none.fl_str_mv Universidade Estadual Paulista Júlio de Mesquita Filho
publisher.none.fl_str_mv Universidade Estadual Paulista Júlio de Mesquita Filho
dc.source.none.fl_str_mv SciELO
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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