Validation of an HPLC method for quantitative determination of Benzocaine in PHBV-microparticles and PLA-nanoparticles
Autor(a) principal: | |
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Data de Publicação: | 2009 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://hdl.handle.net/11449/225583 |
Resumo: | Benzocaine (BZC) is an ester-type local anesthetic used mainly in topical, dermal and mucosal formulations. The present work consists of the development and validation of analytical methodology for evaluation of benzocaine content in the micro and nanoparticles produced by biodegradable polymers (polyhydroxybutyrate-co-hydroxyvalerate and poly-(L-lactide) by HPLC. The validation was done using the reversed-phase C18 column, using a mobile phase consisting of acetonitrile/water 50:50 (v/v), flow rate of 1.5 mL/min and UV-vis detector at 285 nm. The results here obtained showed that the analytical methodology is accurate, reproducible, robust and linear over the molar range concentration of 10-100 μM of benzocaine. The limit of quantification and detection was 13.06 μM and 3.92 μM, respectively. The encapsulation efficiency of benzocaine in PHBV microspheres and PLA nanocapsule were 40% and 70%, respectively. The method developed was applied in the analysis of benzocaine in micro and nanoparticle systems and showed to be efficient, yielding good results. Here, this method was used to evaluate the encapsulation efficiency of benzocaine and will be used in next studies with different micro/ nanoparticle formulations. |
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Validation of an HPLC method for quantitative determination of Benzocaine in PHBV-microparticles and PLA-nanoparticlesBenzocaineHPLCLocal anestheticMicrospheresNanocapsulesBenzocaine (BZC) is an ester-type local anesthetic used mainly in topical, dermal and mucosal formulations. The present work consists of the development and validation of analytical methodology for evaluation of benzocaine content in the micro and nanoparticles produced by biodegradable polymers (polyhydroxybutyrate-co-hydroxyvalerate and poly-(L-lactide) by HPLC. The validation was done using the reversed-phase C18 column, using a mobile phase consisting of acetonitrile/water 50:50 (v/v), flow rate of 1.5 mL/min and UV-vis detector at 285 nm. The results here obtained showed that the analytical methodology is accurate, reproducible, robust and linear over the molar range concentration of 10-100 μM of benzocaine. The limit of quantification and detection was 13.06 μM and 3.92 μM, respectively. The encapsulation efficiency of benzocaine in PHBV microspheres and PLA nanocapsule were 40% and 70%, respectively. The method developed was applied in the analysis of benzocaine in micro and nanoparticle systems and showed to be efficient, yielding good results. Here, this method was used to evaluate the encapsulation efficiency of benzocaine and will be used in next studies with different micro/ nanoparticle formulations.Departamento de Engenharia Ambiental Universidade Estadual Paulista Júlio de Mesquita Filho, Campus Sorocaba, Av. Três de Março, 511, 18087-180 Sorocaba, São PauloDepartamento de Bioquímica Instituto de Biologia Unicamp, Cidade Universitária Zeferino Vaz, s/n, Campinas, SPCentro de Ciências Humana e Natural Universidade Federal do ABC UFABC, Santo André, SPDepartamento de Física e Química Universidade Estadual Paulista Júlio de Mesquita Filho, Campus Ilha Solteira, Ilha Solteira, São PauloDepartamento de Engenharia Ambiental Universidade Estadual Paulista Júlio de Mesquita Filho, Campus Sorocaba, Av. Três de Março, 511, 18087-180 Sorocaba, São PauloDepartamento de Física e Química Universidade Estadual Paulista Júlio de Mesquita Filho, Campus Ilha Solteira, Ilha Solteira, São PauloUniversidade Estadual Paulista (UNESP)Universidade Estadual de Campinas (UNICAMP)Universidade Federal do ABC (UFABC)Grillo, Renato [UNESP]De Melo, Nathalie F.S. [UNESP]De Araújo, Daniele R.De Paula, EneidaDias Filho, Newton L. [UNESP]Rosa, André H. [UNESP]Fraceto, Leonardo F. [UNESP]2022-04-28T20:54:50Z2022-04-28T20:54:50Z2009-08-20info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article393-399Latin American Journal of Pharmacy, v. 28, n. 3, p. 393-399, 2009.0326-2383http://hdl.handle.net/11449/2255832-s2.0-68749114025Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengLatin American Journal of Pharmacyinfo:eu-repo/semantics/openAccess2024-07-10T14:07:47Zoai:repositorio.unesp.br:11449/225583Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T19:09:37.885325Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Validation of an HPLC method for quantitative determination of Benzocaine in PHBV-microparticles and PLA-nanoparticles |
title |
Validation of an HPLC method for quantitative determination of Benzocaine in PHBV-microparticles and PLA-nanoparticles |
spellingShingle |
Validation of an HPLC method for quantitative determination of Benzocaine in PHBV-microparticles and PLA-nanoparticles Grillo, Renato [UNESP] Benzocaine HPLC Local anesthetic Microspheres Nanocapsules |
title_short |
Validation of an HPLC method for quantitative determination of Benzocaine in PHBV-microparticles and PLA-nanoparticles |
title_full |
Validation of an HPLC method for quantitative determination of Benzocaine in PHBV-microparticles and PLA-nanoparticles |
title_fullStr |
Validation of an HPLC method for quantitative determination of Benzocaine in PHBV-microparticles and PLA-nanoparticles |
title_full_unstemmed |
Validation of an HPLC method for quantitative determination of Benzocaine in PHBV-microparticles and PLA-nanoparticles |
title_sort |
Validation of an HPLC method for quantitative determination of Benzocaine in PHBV-microparticles and PLA-nanoparticles |
author |
Grillo, Renato [UNESP] |
author_facet |
Grillo, Renato [UNESP] De Melo, Nathalie F.S. [UNESP] De Araújo, Daniele R. De Paula, Eneida Dias Filho, Newton L. [UNESP] Rosa, André H. [UNESP] Fraceto, Leonardo F. [UNESP] |
author_role |
author |
author2 |
De Melo, Nathalie F.S. [UNESP] De Araújo, Daniele R. De Paula, Eneida Dias Filho, Newton L. [UNESP] Rosa, André H. [UNESP] Fraceto, Leonardo F. [UNESP] |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) Universidade Estadual de Campinas (UNICAMP) Universidade Federal do ABC (UFABC) |
dc.contributor.author.fl_str_mv |
Grillo, Renato [UNESP] De Melo, Nathalie F.S. [UNESP] De Araújo, Daniele R. De Paula, Eneida Dias Filho, Newton L. [UNESP] Rosa, André H. [UNESP] Fraceto, Leonardo F. [UNESP] |
dc.subject.por.fl_str_mv |
Benzocaine HPLC Local anesthetic Microspheres Nanocapsules |
topic |
Benzocaine HPLC Local anesthetic Microspheres Nanocapsules |
description |
Benzocaine (BZC) is an ester-type local anesthetic used mainly in topical, dermal and mucosal formulations. The present work consists of the development and validation of analytical methodology for evaluation of benzocaine content in the micro and nanoparticles produced by biodegradable polymers (polyhydroxybutyrate-co-hydroxyvalerate and poly-(L-lactide) by HPLC. The validation was done using the reversed-phase C18 column, using a mobile phase consisting of acetonitrile/water 50:50 (v/v), flow rate of 1.5 mL/min and UV-vis detector at 285 nm. The results here obtained showed that the analytical methodology is accurate, reproducible, robust and linear over the molar range concentration of 10-100 μM of benzocaine. The limit of quantification and detection was 13.06 μM and 3.92 μM, respectively. The encapsulation efficiency of benzocaine in PHBV microspheres and PLA nanocapsule were 40% and 70%, respectively. The method developed was applied in the analysis of benzocaine in micro and nanoparticle systems and showed to be efficient, yielding good results. Here, this method was used to evaluate the encapsulation efficiency of benzocaine and will be used in next studies with different micro/ nanoparticle formulations. |
publishDate |
2009 |
dc.date.none.fl_str_mv |
2009-08-20 2022-04-28T20:54:50Z 2022-04-28T20:54:50Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Latin American Journal of Pharmacy, v. 28, n. 3, p. 393-399, 2009. 0326-2383 http://hdl.handle.net/11449/225583 2-s2.0-68749114025 |
identifier_str_mv |
Latin American Journal of Pharmacy, v. 28, n. 3, p. 393-399, 2009. 0326-2383 2-s2.0-68749114025 |
url |
http://hdl.handle.net/11449/225583 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Latin American Journal of Pharmacy |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
393-399 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129027962044416 |