Mycobacterium tuberculosis and Paracoccidioides brasiliensis Formation and Treatment of Mixed Biofilm In Vitro
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.3389/fcimb.2021.681131 http://hdl.handle.net/11449/229889 |
Resumo: | Co-infection of Mycobacterium tuberculosis and Paracoccidioides brasiliensis, present in 20% in Latin America, is a public health problem due to a lack of adequate diagnosis. These microorganisms are capable of forming biofilms, mainly in immunocompromised patients, which can lead to death due to the lack of effective treatment for both diseases. The present research aims to show for the first time the formation of mixed biofilms of M. tuberculosis and P. brasiliensis (Pb18) in vitro, as well as to evaluate the action of 3’hydroxychalcone (3’chalc) -loaded nanoemulsion (NE) (NE3’chalc) against monospecies and mixed biofilms, the formation of mixed biofilms of M. tuberculosis H37Rv (ATCC 27294), 40Rv (clinical strains) and P. brasiliensis (Pb18) (ATCC 32069), and the first condition of formation (H37Rv +Pb18) and (40Rv + Pb18) and second condition of formation (Pb18 + H37Rv) with 45 days of total formation time under both conditions. The results of mixed biofilms (H37Rv + Pb18) and (40Rv + Pb18), showed an organized network of M. tuberculosis bacilli in which P. brasiliensis yeasts are connected with a highly extracellular polysaccharide matrix. The (Pb18 + H37Rv) showed a dense biofilm with an apparent predominance of P. brasiliensis and fragments of M. tuberculosis. PCR assays confirmed the presence of the microorganisms involved in this formation. The characterization of NE and NE3’chalc displayed sizes from 145.00 ± 1.05 and 151.25 ± 0.60, a polydispersity index (PDI) from 0.20± 0.01 to 0.16± 0.01, and zeta potential -58.20 ± 0.92 mV and -56.10 ± 0.71 mV, respectively. The atomic force microscopy (AFM) results showed lamellar structures characteristic of NE. The minimum inhibitory concentration (MIC) values of 3’hidroxychalcone (3’chalc) range from 0.97- 7.8 µg/mL and NE3’chalc from 0.24 - 3.9 µg/mL improved the antibacterial activity when compared with 3’chalc-free, no cytotoxicity. Antibiofilm assays proved the efficacy of 3’chalc-free incorporation in NE. These findings contribute to a greater understanding of the formation of M. tuberculosis and P. brasiliensis in the mixed biofilm. In addition, the findings present a new possible NE3’chalc treatment alternative for the mixed biofilms of these microorganisms, with a high degree of relevance due to the lack of other treatments for these comorbidities. |
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Mycobacterium tuberculosis and Paracoccidioides brasiliensis Formation and Treatment of Mixed Biofilm In Vitro3’hydroxychalconemixed biofilmMycobacterium tuberculosisnanoemulsionParacoccidioides brasiliensisCo-infection of Mycobacterium tuberculosis and Paracoccidioides brasiliensis, present in 20% in Latin America, is a public health problem due to a lack of adequate diagnosis. These microorganisms are capable of forming biofilms, mainly in immunocompromised patients, which can lead to death due to the lack of effective treatment for both diseases. The present research aims to show for the first time the formation of mixed biofilms of M. tuberculosis and P. brasiliensis (Pb18) in vitro, as well as to evaluate the action of 3’hydroxychalcone (3’chalc) -loaded nanoemulsion (NE) (NE3’chalc) against monospecies and mixed biofilms, the formation of mixed biofilms of M. tuberculosis H37Rv (ATCC 27294), 40Rv (clinical strains) and P. brasiliensis (Pb18) (ATCC 32069), and the first condition of formation (H37Rv +Pb18) and (40Rv + Pb18) and second condition of formation (Pb18 + H37Rv) with 45 days of total formation time under both conditions. The results of mixed biofilms (H37Rv + Pb18) and (40Rv + Pb18), showed an organized network of M. tuberculosis bacilli in which P. brasiliensis yeasts are connected with a highly extracellular polysaccharide matrix. The (Pb18 + H37Rv) showed a dense biofilm with an apparent predominance of P. brasiliensis and fragments of M. tuberculosis. PCR assays confirmed the presence of the microorganisms involved in this formation. The characterization of NE and NE3’chalc displayed sizes from 145.00 ± 1.05 and 151.25 ± 0.60, a polydispersity index (PDI) from 0.20± 0.01 to 0.16± 0.01, and zeta potential -58.20 ± 0.92 mV and -56.10 ± 0.71 mV, respectively. The atomic force microscopy (AFM) results showed lamellar structures characteristic of NE. The minimum inhibitory concentration (MIC) values of 3’hidroxychalcone (3’chalc) range from 0.97- 7.8 µg/mL and NE3’chalc from 0.24 - 3.9 µg/mL improved the antibacterial activity when compared with 3’chalc-free, no cytotoxicity. Antibiofilm assays proved the efficacy of 3’chalc-free incorporation in NE. These findings contribute to a greater understanding of the formation of M. tuberculosis and P. brasiliensis in the mixed biofilm. In addition, the findings present a new possible NE3’chalc treatment alternative for the mixed biofilms of these microorganisms, with a high degree of relevance due to the lack of other treatments for these comorbidities.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)School of Pharmaceutical Sciences Department of Clinical Analysis Universidade Estadual Paulista (UNESP)Institute of Physics of Sao Carlos (IFSC) University of Sao Paulo (USP) IFSC/USPExact Sciences and Engineering Paulista Central University Center (UNICEP)Department of Chemistry and Environmental Sciences Institute of Biosciences Humanities and Exact Sciences Universidade Estadual PaulistaDepartment of Drug and Medicines School of Pharmaceutical Sciences Universidade Estadual PaulistaDepartment of Biological School of Pharmaceutical Sciences Universidade Estadual PaulistaSchool of Pharmaceutical Sciences Department of Clinical Analysis Universidade Estadual Paulista (UNESP)Department of Chemistry and Environmental Sciences Institute of Biosciences Humanities and Exact Sciences Universidade Estadual PaulistaDepartment of Drug and Medicines School of Pharmaceutical Sciences Universidade Estadual PaulistaDepartment of Biological School of Pharmaceutical Sciences Universidade Estadual PaulistaUniversidade Estadual Paulista (UNESP)Universidade de São Paulo (USP)Paulista Central University Center (UNICEP)Medina-Alarcón, Kaila Petronila [UNESP]Tobias da Silva, Iara Pengo [UNESP]Ferin, Giovana Garcia [UNESP]Pereira-da-Silva, Marcelo A.Marcos, Caroline Maria [UNESP]dos Santos, Mariana Bastos [UNESP]Regasini, Luis Octávio [UNESP]Chorilli, Marlus [UNESP]Mendes-Giannini, Maria José S. [UNESP]Pavan, Fernando Rogerio [UNESP]Fusco-Almeida, Ana Marisa [UNESP]2022-04-29T08:36:22Z2022-04-29T08:36:22Z2021-11-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.3389/fcimb.2021.681131Frontiers in Cellular and Infection Microbiology, v. 11.2235-2988http://hdl.handle.net/11449/22988910.3389/fcimb.2021.6811312-s2.0-85119108865Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengFrontiers in Cellular and Infection Microbiologyinfo:eu-repo/semantics/openAccess2024-06-21T15:19:09Zoai:repositorio.unesp.br:11449/229889Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T20:28:44.412773Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Mycobacterium tuberculosis and Paracoccidioides brasiliensis Formation and Treatment of Mixed Biofilm In Vitro |
title |
Mycobacterium tuberculosis and Paracoccidioides brasiliensis Formation and Treatment of Mixed Biofilm In Vitro |
spellingShingle |
Mycobacterium tuberculosis and Paracoccidioides brasiliensis Formation and Treatment of Mixed Biofilm In Vitro Medina-Alarcón, Kaila Petronila [UNESP] 3’hydroxychalcone mixed biofilm Mycobacterium tuberculosis nanoemulsion Paracoccidioides brasiliensis |
title_short |
Mycobacterium tuberculosis and Paracoccidioides brasiliensis Formation and Treatment of Mixed Biofilm In Vitro |
title_full |
Mycobacterium tuberculosis and Paracoccidioides brasiliensis Formation and Treatment of Mixed Biofilm In Vitro |
title_fullStr |
Mycobacterium tuberculosis and Paracoccidioides brasiliensis Formation and Treatment of Mixed Biofilm In Vitro |
title_full_unstemmed |
Mycobacterium tuberculosis and Paracoccidioides brasiliensis Formation and Treatment of Mixed Biofilm In Vitro |
title_sort |
Mycobacterium tuberculosis and Paracoccidioides brasiliensis Formation and Treatment of Mixed Biofilm In Vitro |
author |
Medina-Alarcón, Kaila Petronila [UNESP] |
author_facet |
Medina-Alarcón, Kaila Petronila [UNESP] Tobias da Silva, Iara Pengo [UNESP] Ferin, Giovana Garcia [UNESP] Pereira-da-Silva, Marcelo A. Marcos, Caroline Maria [UNESP] dos Santos, Mariana Bastos [UNESP] Regasini, Luis Octávio [UNESP] Chorilli, Marlus [UNESP] Mendes-Giannini, Maria José S. [UNESP] Pavan, Fernando Rogerio [UNESP] Fusco-Almeida, Ana Marisa [UNESP] |
author_role |
author |
author2 |
Tobias da Silva, Iara Pengo [UNESP] Ferin, Giovana Garcia [UNESP] Pereira-da-Silva, Marcelo A. Marcos, Caroline Maria [UNESP] dos Santos, Mariana Bastos [UNESP] Regasini, Luis Octávio [UNESP] Chorilli, Marlus [UNESP] Mendes-Giannini, Maria José S. [UNESP] Pavan, Fernando Rogerio [UNESP] Fusco-Almeida, Ana Marisa [UNESP] |
author2_role |
author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) Universidade de São Paulo (USP) Paulista Central University Center (UNICEP) |
dc.contributor.author.fl_str_mv |
Medina-Alarcón, Kaila Petronila [UNESP] Tobias da Silva, Iara Pengo [UNESP] Ferin, Giovana Garcia [UNESP] Pereira-da-Silva, Marcelo A. Marcos, Caroline Maria [UNESP] dos Santos, Mariana Bastos [UNESP] Regasini, Luis Octávio [UNESP] Chorilli, Marlus [UNESP] Mendes-Giannini, Maria José S. [UNESP] Pavan, Fernando Rogerio [UNESP] Fusco-Almeida, Ana Marisa [UNESP] |
dc.subject.por.fl_str_mv |
3’hydroxychalcone mixed biofilm Mycobacterium tuberculosis nanoemulsion Paracoccidioides brasiliensis |
topic |
3’hydroxychalcone mixed biofilm Mycobacterium tuberculosis nanoemulsion Paracoccidioides brasiliensis |
description |
Co-infection of Mycobacterium tuberculosis and Paracoccidioides brasiliensis, present in 20% in Latin America, is a public health problem due to a lack of adequate diagnosis. These microorganisms are capable of forming biofilms, mainly in immunocompromised patients, which can lead to death due to the lack of effective treatment for both diseases. The present research aims to show for the first time the formation of mixed biofilms of M. tuberculosis and P. brasiliensis (Pb18) in vitro, as well as to evaluate the action of 3’hydroxychalcone (3’chalc) -loaded nanoemulsion (NE) (NE3’chalc) against monospecies and mixed biofilms, the formation of mixed biofilms of M. tuberculosis H37Rv (ATCC 27294), 40Rv (clinical strains) and P. brasiliensis (Pb18) (ATCC 32069), and the first condition of formation (H37Rv +Pb18) and (40Rv + Pb18) and second condition of formation (Pb18 + H37Rv) with 45 days of total formation time under both conditions. The results of mixed biofilms (H37Rv + Pb18) and (40Rv + Pb18), showed an organized network of M. tuberculosis bacilli in which P. brasiliensis yeasts are connected with a highly extracellular polysaccharide matrix. The (Pb18 + H37Rv) showed a dense biofilm with an apparent predominance of P. brasiliensis and fragments of M. tuberculosis. PCR assays confirmed the presence of the microorganisms involved in this formation. The characterization of NE and NE3’chalc displayed sizes from 145.00 ± 1.05 and 151.25 ± 0.60, a polydispersity index (PDI) from 0.20± 0.01 to 0.16± 0.01, and zeta potential -58.20 ± 0.92 mV and -56.10 ± 0.71 mV, respectively. The atomic force microscopy (AFM) results showed lamellar structures characteristic of NE. The minimum inhibitory concentration (MIC) values of 3’hidroxychalcone (3’chalc) range from 0.97- 7.8 µg/mL and NE3’chalc from 0.24 - 3.9 µg/mL improved the antibacterial activity when compared with 3’chalc-free, no cytotoxicity. Antibiofilm assays proved the efficacy of 3’chalc-free incorporation in NE. These findings contribute to a greater understanding of the formation of M. tuberculosis and P. brasiliensis in the mixed biofilm. In addition, the findings present a new possible NE3’chalc treatment alternative for the mixed biofilms of these microorganisms, with a high degree of relevance due to the lack of other treatments for these comorbidities. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-11-01 2022-04-29T08:36:22Z 2022-04-29T08:36:22Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.3389/fcimb.2021.681131 Frontiers in Cellular and Infection Microbiology, v. 11. 2235-2988 http://hdl.handle.net/11449/229889 10.3389/fcimb.2021.681131 2-s2.0-85119108865 |
url |
http://dx.doi.org/10.3389/fcimb.2021.681131 http://hdl.handle.net/11449/229889 |
identifier_str_mv |
Frontiers in Cellular and Infection Microbiology, v. 11. 2235-2988 10.3389/fcimb.2021.681131 2-s2.0-85119108865 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Frontiers in Cellular and Infection Microbiology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129208151441408 |