Mycobacterium tuberculosis and Paracoccidioides brasiliensis Formation and Treatment of Mixed Biofilm In Vitro

Detalhes bibliográficos
Autor(a) principal: Medina-Alarcón, Kaila Petronila [UNESP]
Data de Publicação: 2021
Outros Autores: Tobias da Silva, Iara Pengo [UNESP], Ferin, Giovana Garcia [UNESP], Pereira-da-Silva, Marcelo A., Marcos, Caroline Maria [UNESP], dos Santos, Mariana Bastos [UNESP], Regasini, Luis Octávio [UNESP], Chorilli, Marlus [UNESP], Mendes-Giannini, Maria José S. [UNESP], Pavan, Fernando Rogerio [UNESP], Fusco-Almeida, Ana Marisa [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.3389/fcimb.2021.681131
http://hdl.handle.net/11449/229889
Resumo: Co-infection of Mycobacterium tuberculosis and Paracoccidioides brasiliensis, present in 20% in Latin America, is a public health problem due to a lack of adequate diagnosis. These microorganisms are capable of forming biofilms, mainly in immunocompromised patients, which can lead to death due to the lack of effective treatment for both diseases. The present research aims to show for the first time the formation of mixed biofilms of M. tuberculosis and P. brasiliensis (Pb18) in vitro, as well as to evaluate the action of 3’hydroxychalcone (3’chalc) -loaded nanoemulsion (NE) (NE3’chalc) against monospecies and mixed biofilms, the formation of mixed biofilms of M. tuberculosis H37Rv (ATCC 27294), 40Rv (clinical strains) and P. brasiliensis (Pb18) (ATCC 32069), and the first condition of formation (H37Rv +Pb18) and (40Rv + Pb18) and second condition of formation (Pb18 + H37Rv) with 45 days of total formation time under both conditions. The results of mixed biofilms (H37Rv + Pb18) and (40Rv + Pb18), showed an organized network of M. tuberculosis bacilli in which P. brasiliensis yeasts are connected with a highly extracellular polysaccharide matrix. The (Pb18 + H37Rv) showed a dense biofilm with an apparent predominance of P. brasiliensis and fragments of M. tuberculosis. PCR assays confirmed the presence of the microorganisms involved in this formation. The characterization of NE and NE3’chalc displayed sizes from 145.00 ± 1.05 and 151.25 ± 0.60, a polydispersity index (PDI) from 0.20± 0.01 to 0.16± 0.01, and zeta potential -58.20 ± 0.92 mV and -56.10 ± 0.71 mV, respectively. The atomic force microscopy (AFM) results showed lamellar structures characteristic of NE. The minimum inhibitory concentration (MIC) values of 3’hidroxychalcone (3’chalc) range from 0.97- 7.8 µg/mL and NE3’chalc from 0.24 - 3.9 µg/mL improved the antibacterial activity when compared with 3’chalc-free, no cytotoxicity. Antibiofilm assays proved the efficacy of 3’chalc-free incorporation in NE. These findings contribute to a greater understanding of the formation of M. tuberculosis and P. brasiliensis in the mixed biofilm. In addition, the findings present a new possible NE3’chalc treatment alternative for the mixed biofilms of these microorganisms, with a high degree of relevance due to the lack of other treatments for these comorbidities.
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spelling Mycobacterium tuberculosis and Paracoccidioides brasiliensis Formation and Treatment of Mixed Biofilm In Vitro3’hydroxychalconemixed biofilmMycobacterium tuberculosisnanoemulsionParacoccidioides brasiliensisCo-infection of Mycobacterium tuberculosis and Paracoccidioides brasiliensis, present in 20% in Latin America, is a public health problem due to a lack of adequate diagnosis. These microorganisms are capable of forming biofilms, mainly in immunocompromised patients, which can lead to death due to the lack of effective treatment for both diseases. The present research aims to show for the first time the formation of mixed biofilms of M. tuberculosis and P. brasiliensis (Pb18) in vitro, as well as to evaluate the action of 3’hydroxychalcone (3’chalc) -loaded nanoemulsion (NE) (NE3’chalc) against monospecies and mixed biofilms, the formation of mixed biofilms of M. tuberculosis H37Rv (ATCC 27294), 40Rv (clinical strains) and P. brasiliensis (Pb18) (ATCC 32069), and the first condition of formation (H37Rv +Pb18) and (40Rv + Pb18) and second condition of formation (Pb18 + H37Rv) with 45 days of total formation time under both conditions. The results of mixed biofilms (H37Rv + Pb18) and (40Rv + Pb18), showed an organized network of M. tuberculosis bacilli in which P. brasiliensis yeasts are connected with a highly extracellular polysaccharide matrix. The (Pb18 + H37Rv) showed a dense biofilm with an apparent predominance of P. brasiliensis and fragments of M. tuberculosis. PCR assays confirmed the presence of the microorganisms involved in this formation. The characterization of NE and NE3’chalc displayed sizes from 145.00 ± 1.05 and 151.25 ± 0.60, a polydispersity index (PDI) from 0.20± 0.01 to 0.16± 0.01, and zeta potential -58.20 ± 0.92 mV and -56.10 ± 0.71 mV, respectively. The atomic force microscopy (AFM) results showed lamellar structures characteristic of NE. The minimum inhibitory concentration (MIC) values of 3’hidroxychalcone (3’chalc) range from 0.97- 7.8 µg/mL and NE3’chalc from 0.24 - 3.9 µg/mL improved the antibacterial activity when compared with 3’chalc-free, no cytotoxicity. Antibiofilm assays proved the efficacy of 3’chalc-free incorporation in NE. These findings contribute to a greater understanding of the formation of M. tuberculosis and P. brasiliensis in the mixed biofilm. In addition, the findings present a new possible NE3’chalc treatment alternative for the mixed biofilms of these microorganisms, with a high degree of relevance due to the lack of other treatments for these comorbidities.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)School of Pharmaceutical Sciences Department of Clinical Analysis Universidade Estadual Paulista (UNESP)Institute of Physics of Sao Carlos (IFSC) University of Sao Paulo (USP) IFSC/USPExact Sciences and Engineering Paulista Central University Center (UNICEP)Department of Chemistry and Environmental Sciences Institute of Biosciences Humanities and Exact Sciences Universidade Estadual PaulistaDepartment of Drug and Medicines School of Pharmaceutical Sciences Universidade Estadual PaulistaDepartment of Biological School of Pharmaceutical Sciences Universidade Estadual PaulistaSchool of Pharmaceutical Sciences Department of Clinical Analysis Universidade Estadual Paulista (UNESP)Department of Chemistry and Environmental Sciences Institute of Biosciences Humanities and Exact Sciences Universidade Estadual PaulistaDepartment of Drug and Medicines School of Pharmaceutical Sciences Universidade Estadual PaulistaDepartment of Biological School of Pharmaceutical Sciences Universidade Estadual PaulistaUniversidade Estadual Paulista (UNESP)Universidade de São Paulo (USP)Paulista Central University Center (UNICEP)Medina-Alarcón, Kaila Petronila [UNESP]Tobias da Silva, Iara Pengo [UNESP]Ferin, Giovana Garcia [UNESP]Pereira-da-Silva, Marcelo A.Marcos, Caroline Maria [UNESP]dos Santos, Mariana Bastos [UNESP]Regasini, Luis Octávio [UNESP]Chorilli, Marlus [UNESP]Mendes-Giannini, Maria José S. [UNESP]Pavan, Fernando Rogerio [UNESP]Fusco-Almeida, Ana Marisa [UNESP]2022-04-29T08:36:22Z2022-04-29T08:36:22Z2021-11-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.3389/fcimb.2021.681131Frontiers in Cellular and Infection Microbiology, v. 11.2235-2988http://hdl.handle.net/11449/22988910.3389/fcimb.2021.6811312-s2.0-85119108865Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengFrontiers in Cellular and Infection Microbiologyinfo:eu-repo/semantics/openAccess2024-06-21T15:19:09Zoai:repositorio.unesp.br:11449/229889Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T20:28:44.412773Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Mycobacterium tuberculosis and Paracoccidioides brasiliensis Formation and Treatment of Mixed Biofilm In Vitro
title Mycobacterium tuberculosis and Paracoccidioides brasiliensis Formation and Treatment of Mixed Biofilm In Vitro
spellingShingle Mycobacterium tuberculosis and Paracoccidioides brasiliensis Formation and Treatment of Mixed Biofilm In Vitro
Medina-Alarcón, Kaila Petronila [UNESP]
3’hydroxychalcone
mixed biofilm
Mycobacterium tuberculosis
nanoemulsion
Paracoccidioides brasiliensis
title_short Mycobacterium tuberculosis and Paracoccidioides brasiliensis Formation and Treatment of Mixed Biofilm In Vitro
title_full Mycobacterium tuberculosis and Paracoccidioides brasiliensis Formation and Treatment of Mixed Biofilm In Vitro
title_fullStr Mycobacterium tuberculosis and Paracoccidioides brasiliensis Formation and Treatment of Mixed Biofilm In Vitro
title_full_unstemmed Mycobacterium tuberculosis and Paracoccidioides brasiliensis Formation and Treatment of Mixed Biofilm In Vitro
title_sort Mycobacterium tuberculosis and Paracoccidioides brasiliensis Formation and Treatment of Mixed Biofilm In Vitro
author Medina-Alarcón, Kaila Petronila [UNESP]
author_facet Medina-Alarcón, Kaila Petronila [UNESP]
Tobias da Silva, Iara Pengo [UNESP]
Ferin, Giovana Garcia [UNESP]
Pereira-da-Silva, Marcelo A.
Marcos, Caroline Maria [UNESP]
dos Santos, Mariana Bastos [UNESP]
Regasini, Luis Octávio [UNESP]
Chorilli, Marlus [UNESP]
Mendes-Giannini, Maria José S. [UNESP]
Pavan, Fernando Rogerio [UNESP]
Fusco-Almeida, Ana Marisa [UNESP]
author_role author
author2 Tobias da Silva, Iara Pengo [UNESP]
Ferin, Giovana Garcia [UNESP]
Pereira-da-Silva, Marcelo A.
Marcos, Caroline Maria [UNESP]
dos Santos, Mariana Bastos [UNESP]
Regasini, Luis Octávio [UNESP]
Chorilli, Marlus [UNESP]
Mendes-Giannini, Maria José S. [UNESP]
Pavan, Fernando Rogerio [UNESP]
Fusco-Almeida, Ana Marisa [UNESP]
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (UNESP)
Universidade de São Paulo (USP)
Paulista Central University Center (UNICEP)
dc.contributor.author.fl_str_mv Medina-Alarcón, Kaila Petronila [UNESP]
Tobias da Silva, Iara Pengo [UNESP]
Ferin, Giovana Garcia [UNESP]
Pereira-da-Silva, Marcelo A.
Marcos, Caroline Maria [UNESP]
dos Santos, Mariana Bastos [UNESP]
Regasini, Luis Octávio [UNESP]
Chorilli, Marlus [UNESP]
Mendes-Giannini, Maria José S. [UNESP]
Pavan, Fernando Rogerio [UNESP]
Fusco-Almeida, Ana Marisa [UNESP]
dc.subject.por.fl_str_mv 3’hydroxychalcone
mixed biofilm
Mycobacterium tuberculosis
nanoemulsion
Paracoccidioides brasiliensis
topic 3’hydroxychalcone
mixed biofilm
Mycobacterium tuberculosis
nanoemulsion
Paracoccidioides brasiliensis
description Co-infection of Mycobacterium tuberculosis and Paracoccidioides brasiliensis, present in 20% in Latin America, is a public health problem due to a lack of adequate diagnosis. These microorganisms are capable of forming biofilms, mainly in immunocompromised patients, which can lead to death due to the lack of effective treatment for both diseases. The present research aims to show for the first time the formation of mixed biofilms of M. tuberculosis and P. brasiliensis (Pb18) in vitro, as well as to evaluate the action of 3’hydroxychalcone (3’chalc) -loaded nanoemulsion (NE) (NE3’chalc) against monospecies and mixed biofilms, the formation of mixed biofilms of M. tuberculosis H37Rv (ATCC 27294), 40Rv (clinical strains) and P. brasiliensis (Pb18) (ATCC 32069), and the first condition of formation (H37Rv +Pb18) and (40Rv + Pb18) and second condition of formation (Pb18 + H37Rv) with 45 days of total formation time under both conditions. The results of mixed biofilms (H37Rv + Pb18) and (40Rv + Pb18), showed an organized network of M. tuberculosis bacilli in which P. brasiliensis yeasts are connected with a highly extracellular polysaccharide matrix. The (Pb18 + H37Rv) showed a dense biofilm with an apparent predominance of P. brasiliensis and fragments of M. tuberculosis. PCR assays confirmed the presence of the microorganisms involved in this formation. The characterization of NE and NE3’chalc displayed sizes from 145.00 ± 1.05 and 151.25 ± 0.60, a polydispersity index (PDI) from 0.20± 0.01 to 0.16± 0.01, and zeta potential -58.20 ± 0.92 mV and -56.10 ± 0.71 mV, respectively. The atomic force microscopy (AFM) results showed lamellar structures characteristic of NE. The minimum inhibitory concentration (MIC) values of 3’hidroxychalcone (3’chalc) range from 0.97- 7.8 µg/mL and NE3’chalc from 0.24 - 3.9 µg/mL improved the antibacterial activity when compared with 3’chalc-free, no cytotoxicity. Antibiofilm assays proved the efficacy of 3’chalc-free incorporation in NE. These findings contribute to a greater understanding of the formation of M. tuberculosis and P. brasiliensis in the mixed biofilm. In addition, the findings present a new possible NE3’chalc treatment alternative for the mixed biofilms of these microorganisms, with a high degree of relevance due to the lack of other treatments for these comorbidities.
publishDate 2021
dc.date.none.fl_str_mv 2021-11-01
2022-04-29T08:36:22Z
2022-04-29T08:36:22Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.3389/fcimb.2021.681131
Frontiers in Cellular and Infection Microbiology, v. 11.
2235-2988
http://hdl.handle.net/11449/229889
10.3389/fcimb.2021.681131
2-s2.0-85119108865
url http://dx.doi.org/10.3389/fcimb.2021.681131
http://hdl.handle.net/11449/229889
identifier_str_mv Frontiers in Cellular and Infection Microbiology, v. 11.
2235-2988
10.3389/fcimb.2021.681131
2-s2.0-85119108865
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Frontiers in Cellular and Infection Microbiology
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
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