Red biocolorant from endophytic Talaromyces minnesotensis: production, properties, and potential applications

Detalhes bibliográficos
Autor(a) principal: Sousa, Marina D. B.
Data de Publicação: 2023
Outros Autores: Pereira, Murilo L., Cruz, Felipe P. N., Romano, Luis H., Albuquerque, Yulli R., Correia, Ricardo O., Oliveira, Fernanda M., Primo, Fernando L. [UNESP], Baptista-Neto, Álvaro [UNESP], Sousa, Cristina P., Anibal, Fernanda F., Moraes, Luiz Alberto B., Badino, Alberto C.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1007/s00253-023-12491-7
http://hdl.handle.net/11449/247215
Resumo: Abstract: Fungal colorants are gradually entering the global color market, given their advantages of being less harmful to human health, as well as having greater stability and biotechnological potential, compared to other natural sources. The present work concerns the isolation and identification of an endophytic filamentous fungus, together with the chemical characterization and assessment of the fluorescence, toxicity, stability, and application potential of its synthesized red colorant. The endophytic fungus was isolated from Hymenaea courbaril, a tree from the Brazilian savannah, and was identified as Talaromyces minnesotensis by phenotypic and genotypic characterization. Submerged cultivation of the fungus resulted in the production of approximately 12 AU500 of a red biocolorant which according to LC-DAD-MS analysis is characterized by being a complex mixture of molecules of the azaphilone class. Regarding cytotoxicity assays, activity against human hepatoblastoma (HepG2) cells was only observed at concentrations above 5.0 g L−1, while antimicrobial effects against pathogenic bacteria and yeast occurred at concentrations above 50.0 g L−1. The biocolorant showed high stability at neutral pH values and low temperatures (10 to 20 °C) and high half-life values (t1/2), which indicates potential versatility for application in different matrices, as observed in tests using detergent, gelatin, enamel, paint, and fabrics. The results demonstrated that the biocolorant synthesized by Talaromyces minnesotensis has potential for future biotechnological applications. Key Points: • An endophytic fungus, which was isolated and identified, synthesize a red colorant. • The colorant showed fluorescence property, low toxicity, and application potential. • The red biocolorant was highly stable at pH 8.0 and temperatures below 20°C. Graphical Abstract: [Figure not available: see fulltext.]
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spelling Red biocolorant from endophytic Talaromyces minnesotensis: production, properties, and potential applicationsColor stabilityEndophytic fungusFungal pigmentRed biocolorantTalaromyces minnesotensisAbstract: Fungal colorants are gradually entering the global color market, given their advantages of being less harmful to human health, as well as having greater stability and biotechnological potential, compared to other natural sources. The present work concerns the isolation and identification of an endophytic filamentous fungus, together with the chemical characterization and assessment of the fluorescence, toxicity, stability, and application potential of its synthesized red colorant. The endophytic fungus was isolated from Hymenaea courbaril, a tree from the Brazilian savannah, and was identified as Talaromyces minnesotensis by phenotypic and genotypic characterization. Submerged cultivation of the fungus resulted in the production of approximately 12 AU500 of a red biocolorant which according to LC-DAD-MS analysis is characterized by being a complex mixture of molecules of the azaphilone class. Regarding cytotoxicity assays, activity against human hepatoblastoma (HepG2) cells was only observed at concentrations above 5.0 g L−1, while antimicrobial effects against pathogenic bacteria and yeast occurred at concentrations above 50.0 g L−1. The biocolorant showed high stability at neutral pH values and low temperatures (10 to 20 °C) and high half-life values (t1/2), which indicates potential versatility for application in different matrices, as observed in tests using detergent, gelatin, enamel, paint, and fabrics. The results demonstrated that the biocolorant synthesized by Talaromyces minnesotensis has potential for future biotechnological applications. Key Points: • An endophytic fungus, which was isolated and identified, synthesize a red colorant. • The colorant showed fluorescence property, low toxicity, and application potential. • The red biocolorant was highly stable at pH 8.0 and temperatures below 20°C. Graphical Abstract: [Figure not available: see fulltext.]Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Graduate Program of Chemical Engineering Department of Chemical Engineering Federal University of São Carlos, C.P. 676, São PauloChemical Engineering Undergraduate Course Department of Chemical Engineering Federal University of São Carlos, São PauloGraduate Program of Biotechnology Department of Morphology and Pathology Federal University of São Carlos, São PauloDepartment of Engineering of Bioprocess and Biotechnology School of Pharmaceutical Sciences São Paulo State University (UNESP), São PauloLaboratory of Microbiology and Biomolecules – LaMiB Department of Morphology and Pathology Federal University of São Carlos, São PauloLaboratory of Inflammation and Infectious Diseases – LIDI Department of Morphology and Pathology Federal University of São Carlos, São PauloGraduate Program of Chemistry Laboratory of Mass Spectrometry Applied to Natural Products Chemistry Department School of Philosophy Sciences and Languages University of São PauloDepartment of Engineering of Bioprocess and Biotechnology School of Pharmaceutical Sciences São Paulo State University (UNESP), São PauloCNPq: 309728/2021-5CAPES: Finance Code 001Universidade Federal de São Carlos (UFSCar)Universidade Estadual Paulista (UNESP)Universidade de São Paulo (USP)Sousa, Marina D. B.Pereira, Murilo L.Cruz, Felipe P. N.Romano, Luis H.Albuquerque, Yulli R.Correia, Ricardo O.Oliveira, Fernanda M.Primo, Fernando L. [UNESP]Baptista-Neto, Álvaro [UNESP]Sousa, Cristina P.Anibal, Fernanda F.Moraes, Luiz Alberto B.Badino, Alberto C.2023-07-29T13:09:53Z2023-07-29T13:09:53Z2023-06-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article3699-3716http://dx.doi.org/10.1007/s00253-023-12491-7Applied Microbiology and Biotechnology, v. 107, n. 11, p. 3699-3716, 2023.1432-06140175-7598http://hdl.handle.net/11449/24721510.1007/s00253-023-12491-72-s2.0-85153113097Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengApplied Microbiology and Biotechnologyinfo:eu-repo/semantics/openAccess2023-07-29T13:09:53Zoai:repositorio.unesp.br:11449/247215Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T20:47:04.488108Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Red biocolorant from endophytic Talaromyces minnesotensis: production, properties, and potential applications
title Red biocolorant from endophytic Talaromyces minnesotensis: production, properties, and potential applications
spellingShingle Red biocolorant from endophytic Talaromyces minnesotensis: production, properties, and potential applications
Sousa, Marina D. B.
Color stability
Endophytic fungus
Fungal pigment
Red biocolorant
Talaromyces minnesotensis
title_short Red biocolorant from endophytic Talaromyces minnesotensis: production, properties, and potential applications
title_full Red biocolorant from endophytic Talaromyces minnesotensis: production, properties, and potential applications
title_fullStr Red biocolorant from endophytic Talaromyces minnesotensis: production, properties, and potential applications
title_full_unstemmed Red biocolorant from endophytic Talaromyces minnesotensis: production, properties, and potential applications
title_sort Red biocolorant from endophytic Talaromyces minnesotensis: production, properties, and potential applications
author Sousa, Marina D. B.
author_facet Sousa, Marina D. B.
Pereira, Murilo L.
Cruz, Felipe P. N.
Romano, Luis H.
Albuquerque, Yulli R.
Correia, Ricardo O.
Oliveira, Fernanda M.
Primo, Fernando L. [UNESP]
Baptista-Neto, Álvaro [UNESP]
Sousa, Cristina P.
Anibal, Fernanda F.
Moraes, Luiz Alberto B.
Badino, Alberto C.
author_role author
author2 Pereira, Murilo L.
Cruz, Felipe P. N.
Romano, Luis H.
Albuquerque, Yulli R.
Correia, Ricardo O.
Oliveira, Fernanda M.
Primo, Fernando L. [UNESP]
Baptista-Neto, Álvaro [UNESP]
Sousa, Cristina P.
Anibal, Fernanda F.
Moraes, Luiz Alberto B.
Badino, Alberto C.
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Federal de São Carlos (UFSCar)
Universidade Estadual Paulista (UNESP)
Universidade de São Paulo (USP)
dc.contributor.author.fl_str_mv Sousa, Marina D. B.
Pereira, Murilo L.
Cruz, Felipe P. N.
Romano, Luis H.
Albuquerque, Yulli R.
Correia, Ricardo O.
Oliveira, Fernanda M.
Primo, Fernando L. [UNESP]
Baptista-Neto, Álvaro [UNESP]
Sousa, Cristina P.
Anibal, Fernanda F.
Moraes, Luiz Alberto B.
Badino, Alberto C.
dc.subject.por.fl_str_mv Color stability
Endophytic fungus
Fungal pigment
Red biocolorant
Talaromyces minnesotensis
topic Color stability
Endophytic fungus
Fungal pigment
Red biocolorant
Talaromyces minnesotensis
description Abstract: Fungal colorants are gradually entering the global color market, given their advantages of being less harmful to human health, as well as having greater stability and biotechnological potential, compared to other natural sources. The present work concerns the isolation and identification of an endophytic filamentous fungus, together with the chemical characterization and assessment of the fluorescence, toxicity, stability, and application potential of its synthesized red colorant. The endophytic fungus was isolated from Hymenaea courbaril, a tree from the Brazilian savannah, and was identified as Talaromyces minnesotensis by phenotypic and genotypic characterization. Submerged cultivation of the fungus resulted in the production of approximately 12 AU500 of a red biocolorant which according to LC-DAD-MS analysis is characterized by being a complex mixture of molecules of the azaphilone class. Regarding cytotoxicity assays, activity against human hepatoblastoma (HepG2) cells was only observed at concentrations above 5.0 g L−1, while antimicrobial effects against pathogenic bacteria and yeast occurred at concentrations above 50.0 g L−1. The biocolorant showed high stability at neutral pH values and low temperatures (10 to 20 °C) and high half-life values (t1/2), which indicates potential versatility for application in different matrices, as observed in tests using detergent, gelatin, enamel, paint, and fabrics. The results demonstrated that the biocolorant synthesized by Talaromyces minnesotensis has potential for future biotechnological applications. Key Points: • An endophytic fungus, which was isolated and identified, synthesize a red colorant. • The colorant showed fluorescence property, low toxicity, and application potential. • The red biocolorant was highly stable at pH 8.0 and temperatures below 20°C. Graphical Abstract: [Figure not available: see fulltext.]
publishDate 2023
dc.date.none.fl_str_mv 2023-07-29T13:09:53Z
2023-07-29T13:09:53Z
2023-06-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1007/s00253-023-12491-7
Applied Microbiology and Biotechnology, v. 107, n. 11, p. 3699-3716, 2023.
1432-0614
0175-7598
http://hdl.handle.net/11449/247215
10.1007/s00253-023-12491-7
2-s2.0-85153113097
url http://dx.doi.org/10.1007/s00253-023-12491-7
http://hdl.handle.net/11449/247215
identifier_str_mv Applied Microbiology and Biotechnology, v. 107, n. 11, p. 3699-3716, 2023.
1432-0614
0175-7598
10.1007/s00253-023-12491-7
2-s2.0-85153113097
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Applied Microbiology and Biotechnology
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 3699-3716
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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