Red biocolorant from endophytic Talaromyces minnesotensis: production, properties, and potential applications
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1007/s00253-023-12491-7 http://hdl.handle.net/11449/247215 |
Resumo: | Abstract: Fungal colorants are gradually entering the global color market, given their advantages of being less harmful to human health, as well as having greater stability and biotechnological potential, compared to other natural sources. The present work concerns the isolation and identification of an endophytic filamentous fungus, together with the chemical characterization and assessment of the fluorescence, toxicity, stability, and application potential of its synthesized red colorant. The endophytic fungus was isolated from Hymenaea courbaril, a tree from the Brazilian savannah, and was identified as Talaromyces minnesotensis by phenotypic and genotypic characterization. Submerged cultivation of the fungus resulted in the production of approximately 12 AU500 of a red biocolorant which according to LC-DAD-MS analysis is characterized by being a complex mixture of molecules of the azaphilone class. Regarding cytotoxicity assays, activity against human hepatoblastoma (HepG2) cells was only observed at concentrations above 5.0 g L−1, while antimicrobial effects against pathogenic bacteria and yeast occurred at concentrations above 50.0 g L−1. The biocolorant showed high stability at neutral pH values and low temperatures (10 to 20 °C) and high half-life values (t1/2), which indicates potential versatility for application in different matrices, as observed in tests using detergent, gelatin, enamel, paint, and fabrics. The results demonstrated that the biocolorant synthesized by Talaromyces minnesotensis has potential for future biotechnological applications. Key Points: • An endophytic fungus, which was isolated and identified, synthesize a red colorant. • The colorant showed fluorescence property, low toxicity, and application potential. • The red biocolorant was highly stable at pH 8.0 and temperatures below 20°C. Graphical Abstract: [Figure not available: see fulltext.] |
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Red biocolorant from endophytic Talaromyces minnesotensis: production, properties, and potential applicationsColor stabilityEndophytic fungusFungal pigmentRed biocolorantTalaromyces minnesotensisAbstract: Fungal colorants are gradually entering the global color market, given their advantages of being less harmful to human health, as well as having greater stability and biotechnological potential, compared to other natural sources. The present work concerns the isolation and identification of an endophytic filamentous fungus, together with the chemical characterization and assessment of the fluorescence, toxicity, stability, and application potential of its synthesized red colorant. The endophytic fungus was isolated from Hymenaea courbaril, a tree from the Brazilian savannah, and was identified as Talaromyces minnesotensis by phenotypic and genotypic characterization. Submerged cultivation of the fungus resulted in the production of approximately 12 AU500 of a red biocolorant which according to LC-DAD-MS analysis is characterized by being a complex mixture of molecules of the azaphilone class. Regarding cytotoxicity assays, activity against human hepatoblastoma (HepG2) cells was only observed at concentrations above 5.0 g L−1, while antimicrobial effects against pathogenic bacteria and yeast occurred at concentrations above 50.0 g L−1. The biocolorant showed high stability at neutral pH values and low temperatures (10 to 20 °C) and high half-life values (t1/2), which indicates potential versatility for application in different matrices, as observed in tests using detergent, gelatin, enamel, paint, and fabrics. The results demonstrated that the biocolorant synthesized by Talaromyces minnesotensis has potential for future biotechnological applications. Key Points: • An endophytic fungus, which was isolated and identified, synthesize a red colorant. • The colorant showed fluorescence property, low toxicity, and application potential. • The red biocolorant was highly stable at pH 8.0 and temperatures below 20°C. Graphical Abstract: [Figure not available: see fulltext.]Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Graduate Program of Chemical Engineering Department of Chemical Engineering Federal University of São Carlos, C.P. 676, São PauloChemical Engineering Undergraduate Course Department of Chemical Engineering Federal University of São Carlos, São PauloGraduate Program of Biotechnology Department of Morphology and Pathology Federal University of São Carlos, São PauloDepartment of Engineering of Bioprocess and Biotechnology School of Pharmaceutical Sciences São Paulo State University (UNESP), São PauloLaboratory of Microbiology and Biomolecules – LaMiB Department of Morphology and Pathology Federal University of São Carlos, São PauloLaboratory of Inflammation and Infectious Diseases – LIDI Department of Morphology and Pathology Federal University of São Carlos, São PauloGraduate Program of Chemistry Laboratory of Mass Spectrometry Applied to Natural Products Chemistry Department School of Philosophy Sciences and Languages University of São PauloDepartment of Engineering of Bioprocess and Biotechnology School of Pharmaceutical Sciences São Paulo State University (UNESP), São PauloCNPq: 309728/2021-5CAPES: Finance Code 001Universidade Federal de São Carlos (UFSCar)Universidade Estadual Paulista (UNESP)Universidade de São Paulo (USP)Sousa, Marina D. B.Pereira, Murilo L.Cruz, Felipe P. N.Romano, Luis H.Albuquerque, Yulli R.Correia, Ricardo O.Oliveira, Fernanda M.Primo, Fernando L. [UNESP]Baptista-Neto, Álvaro [UNESP]Sousa, Cristina P.Anibal, Fernanda F.Moraes, Luiz Alberto B.Badino, Alberto C.2023-07-29T13:09:53Z2023-07-29T13:09:53Z2023-06-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article3699-3716http://dx.doi.org/10.1007/s00253-023-12491-7Applied Microbiology and Biotechnology, v. 107, n. 11, p. 3699-3716, 2023.1432-06140175-7598http://hdl.handle.net/11449/24721510.1007/s00253-023-12491-72-s2.0-85153113097Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengApplied Microbiology and Biotechnologyinfo:eu-repo/semantics/openAccess2023-07-29T13:09:53Zoai:repositorio.unesp.br:11449/247215Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T20:47:04.488108Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Red biocolorant from endophytic Talaromyces minnesotensis: production, properties, and potential applications |
title |
Red biocolorant from endophytic Talaromyces minnesotensis: production, properties, and potential applications |
spellingShingle |
Red biocolorant from endophytic Talaromyces minnesotensis: production, properties, and potential applications Sousa, Marina D. B. Color stability Endophytic fungus Fungal pigment Red biocolorant Talaromyces minnesotensis |
title_short |
Red biocolorant from endophytic Talaromyces minnesotensis: production, properties, and potential applications |
title_full |
Red biocolorant from endophytic Talaromyces minnesotensis: production, properties, and potential applications |
title_fullStr |
Red biocolorant from endophytic Talaromyces minnesotensis: production, properties, and potential applications |
title_full_unstemmed |
Red biocolorant from endophytic Talaromyces minnesotensis: production, properties, and potential applications |
title_sort |
Red biocolorant from endophytic Talaromyces minnesotensis: production, properties, and potential applications |
author |
Sousa, Marina D. B. |
author_facet |
Sousa, Marina D. B. Pereira, Murilo L. Cruz, Felipe P. N. Romano, Luis H. Albuquerque, Yulli R. Correia, Ricardo O. Oliveira, Fernanda M. Primo, Fernando L. [UNESP] Baptista-Neto, Álvaro [UNESP] Sousa, Cristina P. Anibal, Fernanda F. Moraes, Luiz Alberto B. Badino, Alberto C. |
author_role |
author |
author2 |
Pereira, Murilo L. Cruz, Felipe P. N. Romano, Luis H. Albuquerque, Yulli R. Correia, Ricardo O. Oliveira, Fernanda M. Primo, Fernando L. [UNESP] Baptista-Neto, Álvaro [UNESP] Sousa, Cristina P. Anibal, Fernanda F. Moraes, Luiz Alberto B. Badino, Alberto C. |
author2_role |
author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Federal de São Carlos (UFSCar) Universidade Estadual Paulista (UNESP) Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
Sousa, Marina D. B. Pereira, Murilo L. Cruz, Felipe P. N. Romano, Luis H. Albuquerque, Yulli R. Correia, Ricardo O. Oliveira, Fernanda M. Primo, Fernando L. [UNESP] Baptista-Neto, Álvaro [UNESP] Sousa, Cristina P. Anibal, Fernanda F. Moraes, Luiz Alberto B. Badino, Alberto C. |
dc.subject.por.fl_str_mv |
Color stability Endophytic fungus Fungal pigment Red biocolorant Talaromyces minnesotensis |
topic |
Color stability Endophytic fungus Fungal pigment Red biocolorant Talaromyces minnesotensis |
description |
Abstract: Fungal colorants are gradually entering the global color market, given their advantages of being less harmful to human health, as well as having greater stability and biotechnological potential, compared to other natural sources. The present work concerns the isolation and identification of an endophytic filamentous fungus, together with the chemical characterization and assessment of the fluorescence, toxicity, stability, and application potential of its synthesized red colorant. The endophytic fungus was isolated from Hymenaea courbaril, a tree from the Brazilian savannah, and was identified as Talaromyces minnesotensis by phenotypic and genotypic characterization. Submerged cultivation of the fungus resulted in the production of approximately 12 AU500 of a red biocolorant which according to LC-DAD-MS analysis is characterized by being a complex mixture of molecules of the azaphilone class. Regarding cytotoxicity assays, activity against human hepatoblastoma (HepG2) cells was only observed at concentrations above 5.0 g L−1, while antimicrobial effects against pathogenic bacteria and yeast occurred at concentrations above 50.0 g L−1. The biocolorant showed high stability at neutral pH values and low temperatures (10 to 20 °C) and high half-life values (t1/2), which indicates potential versatility for application in different matrices, as observed in tests using detergent, gelatin, enamel, paint, and fabrics. The results demonstrated that the biocolorant synthesized by Talaromyces minnesotensis has potential for future biotechnological applications. Key Points: • An endophytic fungus, which was isolated and identified, synthesize a red colorant. • The colorant showed fluorescence property, low toxicity, and application potential. • The red biocolorant was highly stable at pH 8.0 and temperatures below 20°C. Graphical Abstract: [Figure not available: see fulltext.] |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-07-29T13:09:53Z 2023-07-29T13:09:53Z 2023-06-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/s00253-023-12491-7 Applied Microbiology and Biotechnology, v. 107, n. 11, p. 3699-3716, 2023. 1432-0614 0175-7598 http://hdl.handle.net/11449/247215 10.1007/s00253-023-12491-7 2-s2.0-85153113097 |
url |
http://dx.doi.org/10.1007/s00253-023-12491-7 http://hdl.handle.net/11449/247215 |
identifier_str_mv |
Applied Microbiology and Biotechnology, v. 107, n. 11, p. 3699-3716, 2023. 1432-0614 0175-7598 10.1007/s00253-023-12491-7 2-s2.0-85153113097 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Applied Microbiology and Biotechnology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
3699-3716 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1808129248215433216 |