Estabelecimento in vitro de batata doce (Ipomoea batatas)

• Autor(a) principal: Pelozato, Paula Arriero
• Data de Publicação: 2022
• Tipo de documento: Trabalho de conclusão de curso
• Idioma: por
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://hdl.handle.net/11449/217059
• topic: Bioestimulant; Light spectrum; Cyperus rotundus; Bioestimulante; Espectro de luz

In vitro cultivation is an important tool for the development and improvement of culture, enabling the production of plants with higher quality. The C. rotundus plants have in their bulbs that can be used as biostimulants and phytoregulators. The present study aimed to evaluate the influence of C. rotundus extracts on the development in plants of sweet potato grown in vitro. The work was divided into two parts. The first evaluated the development of explants in MS 50% culture medium with and without extract and different light spectrum; the experimental design was DIC, 4x2 factorial (four light spectrum (mixed, blue, yellow, and red) and two types of culture medium (pure and with the addition of extract) with 3 replications; were evaluated: height, number of leaves per seedling, number of buds per seedling and development; plant height there was no statistical difference between the culture medium and the spectrum with better combination results it was the mixed one. In the second experiment, were tested C. rotundus’ bulbs and leaves extracts; the experimental design was DIC, in a 2x5+1 factorial scheme, two sources, five levels of extract (50, 100, 150, and 250 ml), and a control (MS 50% standard), with 25 repetitions; were evaluated: plant height, number of roots, number of leaves, length of the largest root, number of buds, number of calluses, contamination, leaf color, and explant development; the extract of C. rotundus’ bulbs at a dose of 50 mL was efficient for plant height and the development of explants, and at a dose of 150 mL, for number of leaves and number of roots.