Avaliação da regeneração do músculo esquelético em modelo experimental após desnutrição
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Publication Date: | 2017 |
Other Authors: | , , , , , , |
Format: | Article |
Language: | eng |
Source: | Repositório Institucional da UNESP |
Download full: | http://dx.doi.org/10.1590/1519-6984.10415 http://hdl.handle.net/11449/174392 |
Summary: | The aim of this study was to analyze muscle regeneration after cryoinjury in the tibialis anterior muscle of young rats that were malnourished and then recovered. Forty Wistar rats were divided into a nourished group that received a normal protein diet (14% casein) for 90 days and a malnourished and recovered rats group (MR) that was submitted to 45 days of malnutrition with a hypoproteic diet (6% casein) followed by 45 days of a normal protein diet (14% casein). After the recovery period, all of the animals underwent cryoinjury in the right tibialis anterior muscle and euthanasia after 7, 14 and 21 days. The amount of connective tissue and the inflammation area was higher in the malnutrition recovered injury MR group (MRI) at 14 days post-injury (p < 0.05). Additionally, the cross-sectional area (CSA) of the regenerated fibers was decreased in the MRI (p < 0.05). The MyoD and myogenin protein levels were higher in the nourished injury group. Similar levels of TGF-β1 were found between groups. The proposed malnutrition protocol was effective in showing delayed changes in the regeneration process of the tibialis anterior muscle of young rats. Furthermore, we observed a delay in muscle repair even after nutritional recovery. |
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Avaliação da regeneração do músculo esquelético em modelo experimental após desnutriçãoEvaluation of skeletal muscle regeneration in experimental model after malnutritionCryolesionMuscle healing processMyogenic regulatory factorsProtein malnutritionSkeletal muscleThe aim of this study was to analyze muscle regeneration after cryoinjury in the tibialis anterior muscle of young rats that were malnourished and then recovered. Forty Wistar rats were divided into a nourished group that received a normal protein diet (14% casein) for 90 days and a malnourished and recovered rats group (MR) that was submitted to 45 days of malnutrition with a hypoproteic diet (6% casein) followed by 45 days of a normal protein diet (14% casein). After the recovery period, all of the animals underwent cryoinjury in the right tibialis anterior muscle and euthanasia after 7, 14 and 21 days. The amount of connective tissue and the inflammation area was higher in the malnutrition recovered injury MR group (MRI) at 14 days post-injury (p < 0.05). Additionally, the cross-sectional area (CSA) of the regenerated fibers was decreased in the MRI (p < 0.05). The MyoD and myogenin protein levels were higher in the nourished injury group. Similar levels of TGF-β1 were found between groups. The proposed malnutrition protocol was effective in showing delayed changes in the regeneration process of the tibialis anterior muscle of young rats. Furthermore, we observed a delay in muscle repair even after nutritional recovery.Laboratory of Neuromuscular Plasticity Graduate Program in Science of Human Movement Universidade Metodista de Piracicaba – UNIMEP, Rodovia do Açúcar, 7000Department of Anatomy Biosciences Institute of Botucatu Universidade Estadual Paulista – UNESP, Distrito de Rubião Júnior, s/nDepartment of Anatomy Biosciences Institute of Botucatu Universidade Estadual Paulista – UNESP, Distrito de Rubião Júnior, s/nUniversidade Metodista de Piracicaba – UNIMEPUniversidade Estadual Paulista (Unesp)Pertille, A.Moura, K. F.Matsumura, C. Y. [UNESP]Ferretti, R. [UNESP]Ramos, D. M.Petrini, A. C.Oliveira, P. C.Silva, C. A.2018-12-11T17:10:54Z2018-12-11T17:10:54Z2017-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article83-91application/pdfhttp://dx.doi.org/10.1590/1519-6984.10415Brazilian Journal of Biology, v. 77, n. 1, p. 83-91, 2017.1678-43751519-6984http://hdl.handle.net/11449/17439210.1590/1519-6984.10415S1519-698420170001000832-s2.0-85016420061S1519-69842017000100083.pdf368161316008617581987719983474830000-0003-3944-19060000-0002-7237-7021Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBrazilian Journal of Biology0,523info:eu-repo/semantics/openAccess2023-11-15T06:11:38Zoai:repositorio.unesp.br:11449/174392Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-11-15T06:11:38Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Avaliação da regeneração do músculo esquelético em modelo experimental após desnutrição Evaluation of skeletal muscle regeneration in experimental model after malnutrition |
title |
Avaliação da regeneração do músculo esquelético em modelo experimental após desnutrição |
spellingShingle |
Avaliação da regeneração do músculo esquelético em modelo experimental após desnutrição Pertille, A. Cryolesion Muscle healing process Myogenic regulatory factors Protein malnutrition Skeletal muscle |
title_short |
Avaliação da regeneração do músculo esquelético em modelo experimental após desnutrição |
title_full |
Avaliação da regeneração do músculo esquelético em modelo experimental após desnutrição |
title_fullStr |
Avaliação da regeneração do músculo esquelético em modelo experimental após desnutrição |
title_full_unstemmed |
Avaliação da regeneração do músculo esquelético em modelo experimental após desnutrição |
title_sort |
Avaliação da regeneração do músculo esquelético em modelo experimental após desnutrição |
author |
Pertille, A. |
author_facet |
Pertille, A. Moura, K. F. Matsumura, C. Y. [UNESP] Ferretti, R. [UNESP] Ramos, D. M. Petrini, A. C. Oliveira, P. C. Silva, C. A. |
author_role |
author |
author2 |
Moura, K. F. Matsumura, C. Y. [UNESP] Ferretti, R. [UNESP] Ramos, D. M. Petrini, A. C. Oliveira, P. C. Silva, C. A. |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Metodista de Piracicaba – UNIMEP Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Pertille, A. Moura, K. F. Matsumura, C. Y. [UNESP] Ferretti, R. [UNESP] Ramos, D. M. Petrini, A. C. Oliveira, P. C. Silva, C. A. |
dc.subject.por.fl_str_mv |
Cryolesion Muscle healing process Myogenic regulatory factors Protein malnutrition Skeletal muscle |
topic |
Cryolesion Muscle healing process Myogenic regulatory factors Protein malnutrition Skeletal muscle |
description |
The aim of this study was to analyze muscle regeneration after cryoinjury in the tibialis anterior muscle of young rats that were malnourished and then recovered. Forty Wistar rats were divided into a nourished group that received a normal protein diet (14% casein) for 90 days and a malnourished and recovered rats group (MR) that was submitted to 45 days of malnutrition with a hypoproteic diet (6% casein) followed by 45 days of a normal protein diet (14% casein). After the recovery period, all of the animals underwent cryoinjury in the right tibialis anterior muscle and euthanasia after 7, 14 and 21 days. The amount of connective tissue and the inflammation area was higher in the malnutrition recovered injury MR group (MRI) at 14 days post-injury (p < 0.05). Additionally, the cross-sectional area (CSA) of the regenerated fibers was decreased in the MRI (p < 0.05). The MyoD and myogenin protein levels were higher in the nourished injury group. Similar levels of TGF-β1 were found between groups. The proposed malnutrition protocol was effective in showing delayed changes in the regeneration process of the tibialis anterior muscle of young rats. Furthermore, we observed a delay in muscle repair even after nutritional recovery. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-01-01 2018-12-11T17:10:54Z 2018-12-11T17:10:54Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/1519-6984.10415 Brazilian Journal of Biology, v. 77, n. 1, p. 83-91, 2017. 1678-4375 1519-6984 http://hdl.handle.net/11449/174392 10.1590/1519-6984.10415 S1519-69842017000100083 2-s2.0-85016420061 S1519-69842017000100083.pdf 3681613160086175 8198771998347483 0000-0003-3944-1906 0000-0002-7237-7021 |
url |
http://dx.doi.org/10.1590/1519-6984.10415 http://hdl.handle.net/11449/174392 |
identifier_str_mv |
Brazilian Journal of Biology, v. 77, n. 1, p. 83-91, 2017. 1678-4375 1519-6984 10.1590/1519-6984.10415 S1519-69842017000100083 2-s2.0-85016420061 S1519-69842017000100083.pdf 3681613160086175 8198771998347483 0000-0003-3944-1906 0000-0002-7237-7021 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Brazilian Journal of Biology 0,523 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
83-91 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1797789710841544704 |