Tuning with Phosphorylcholine Grafts Improves the Physicochemical Properties of PLL/pDNA Nanoparticles at Neutral pH
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1007/s13233-020-8019-y http://hdl.handle.net/11449/201276 |
Resumo: | The improvement of biological properties of polycations is a fundamental step to overcome their limitations as non-viral gene carriers. This work studied the effect of phosphorylcholine (PC) groups on the physicochemical properties of poly(L-lysine) (PLL)/pDNA nanoparticles. Phosphorylcholine-grafted PLL derivatives (PLL-PC) containing increasing proportions of PC were obtained by the reductive amination reaction with phosphoryl glyceraldehyde and characterized by 1H NMR, FTIR, and GPC measurements. The PLL-PC derivatives were used to prepare polyplexes with pDNA and their properties were evaluated by fluorescence, gel electrophoresis and dynamic light scattering (DLS) measurements. The PLL-PC derivatives were able to interact with pDNA at low N/P ratios in physiological pH to form stable polyplexes having lower zeta potentials, as evidenced by the gel electrophoresis and zeta potentials measurements. A degree of grafting of 10% increased the in vitro transfection efficiency of PLL and a degree of 20 mol% of PC groups provided colloidal stability in physiological saline solution at neutral pH. Overall, the PC-PLL derivatives exhibited improved physicochemical properties and have significant potential for further studies as non-viral gene transfer agents. [Figure not available: see fulltext.]. |
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Tuning with Phosphorylcholine Grafts Improves the Physicochemical Properties of PLL/pDNA Nanoparticles at Neutral pHnanoparticlesnon-viral gene therapyphosphorylcholinePLLThe improvement of biological properties of polycations is a fundamental step to overcome their limitations as non-viral gene carriers. This work studied the effect of phosphorylcholine (PC) groups on the physicochemical properties of poly(L-lysine) (PLL)/pDNA nanoparticles. Phosphorylcholine-grafted PLL derivatives (PLL-PC) containing increasing proportions of PC were obtained by the reductive amination reaction with phosphoryl glyceraldehyde and characterized by 1H NMR, FTIR, and GPC measurements. The PLL-PC derivatives were used to prepare polyplexes with pDNA and their properties were evaluated by fluorescence, gel electrophoresis and dynamic light scattering (DLS) measurements. The PLL-PC derivatives were able to interact with pDNA at low N/P ratios in physiological pH to form stable polyplexes having lower zeta potentials, as evidenced by the gel electrophoresis and zeta potentials measurements. A degree of grafting of 10% increased the in vitro transfection efficiency of PLL and a degree of 20 mol% of PC groups provided colloidal stability in physiological saline solution at neutral pH. Overall, the PC-PLL derivatives exhibited improved physicochemical properties and have significant potential for further studies as non-viral gene transfer agents. [Figure not available: see fulltext.].Departamento de Química e Ciências Ambientáis UNESP-Universidade Estadual PaulistaOrthopedic Research Laboratory Hôpital du Sacré-Coeur de Montréal Université de MontréalDepartamento de Química e Ciências Ambientáis UNESP-Universidade Estadual PaulistaUniversidade Estadual Paulista (Unesp)Université de MontréalSemensato, Juliana [UNESP]Fernandes, Júlio CesarBenderdour, Mohamedde Oliveira Tiera, Vera Aparecida [UNESP]Lima, Aline Margarete Furuyama [UNESP]Tiera, Marcio José [UNESP]2020-12-12T02:28:31Z2020-12-12T02:28:31Z2020-02-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article126-135http://dx.doi.org/10.1007/s13233-020-8019-yMacromolecular Research, v. 28, n. 2, p. 126-135, 2020.2092-76731598-5032http://hdl.handle.net/11449/20127610.1007/s13233-020-8019-y2-s2.0-85074046943Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengMacromolecular Researchinfo:eu-repo/semantics/openAccess2021-10-22T14:03:09Zoai:repositorio.unesp.br:11449/201276Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-22T14:03:09Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Tuning with Phosphorylcholine Grafts Improves the Physicochemical Properties of PLL/pDNA Nanoparticles at Neutral pH |
title |
Tuning with Phosphorylcholine Grafts Improves the Physicochemical Properties of PLL/pDNA Nanoparticles at Neutral pH |
spellingShingle |
Tuning with Phosphorylcholine Grafts Improves the Physicochemical Properties of PLL/pDNA Nanoparticles at Neutral pH Semensato, Juliana [UNESP] nanoparticles non-viral gene therapy phosphorylcholine PLL |
title_short |
Tuning with Phosphorylcholine Grafts Improves the Physicochemical Properties of PLL/pDNA Nanoparticles at Neutral pH |
title_full |
Tuning with Phosphorylcholine Grafts Improves the Physicochemical Properties of PLL/pDNA Nanoparticles at Neutral pH |
title_fullStr |
Tuning with Phosphorylcholine Grafts Improves the Physicochemical Properties of PLL/pDNA Nanoparticles at Neutral pH |
title_full_unstemmed |
Tuning with Phosphorylcholine Grafts Improves the Physicochemical Properties of PLL/pDNA Nanoparticles at Neutral pH |
title_sort |
Tuning with Phosphorylcholine Grafts Improves the Physicochemical Properties of PLL/pDNA Nanoparticles at Neutral pH |
author |
Semensato, Juliana [UNESP] |
author_facet |
Semensato, Juliana [UNESP] Fernandes, Júlio Cesar Benderdour, Mohamed de Oliveira Tiera, Vera Aparecida [UNESP] Lima, Aline Margarete Furuyama [UNESP] Tiera, Marcio José [UNESP] |
author_role |
author |
author2 |
Fernandes, Júlio Cesar Benderdour, Mohamed de Oliveira Tiera, Vera Aparecida [UNESP] Lima, Aline Margarete Furuyama [UNESP] Tiera, Marcio José [UNESP] |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Université de Montréal |
dc.contributor.author.fl_str_mv |
Semensato, Juliana [UNESP] Fernandes, Júlio Cesar Benderdour, Mohamed de Oliveira Tiera, Vera Aparecida [UNESP] Lima, Aline Margarete Furuyama [UNESP] Tiera, Marcio José [UNESP] |
dc.subject.por.fl_str_mv |
nanoparticles non-viral gene therapy phosphorylcholine PLL |
topic |
nanoparticles non-viral gene therapy phosphorylcholine PLL |
description |
The improvement of biological properties of polycations is a fundamental step to overcome their limitations as non-viral gene carriers. This work studied the effect of phosphorylcholine (PC) groups on the physicochemical properties of poly(L-lysine) (PLL)/pDNA nanoparticles. Phosphorylcholine-grafted PLL derivatives (PLL-PC) containing increasing proportions of PC were obtained by the reductive amination reaction with phosphoryl glyceraldehyde and characterized by 1H NMR, FTIR, and GPC measurements. The PLL-PC derivatives were used to prepare polyplexes with pDNA and their properties were evaluated by fluorescence, gel electrophoresis and dynamic light scattering (DLS) measurements. The PLL-PC derivatives were able to interact with pDNA at low N/P ratios in physiological pH to form stable polyplexes having lower zeta potentials, as evidenced by the gel electrophoresis and zeta potentials measurements. A degree of grafting of 10% increased the in vitro transfection efficiency of PLL and a degree of 20 mol% of PC groups provided colloidal stability in physiological saline solution at neutral pH. Overall, the PC-PLL derivatives exhibited improved physicochemical properties and have significant potential for further studies as non-viral gene transfer agents. [Figure not available: see fulltext.]. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-12T02:28:31Z 2020-12-12T02:28:31Z 2020-02-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/s13233-020-8019-y Macromolecular Research, v. 28, n. 2, p. 126-135, 2020. 2092-7673 1598-5032 http://hdl.handle.net/11449/201276 10.1007/s13233-020-8019-y 2-s2.0-85074046943 |
url |
http://dx.doi.org/10.1007/s13233-020-8019-y http://hdl.handle.net/11449/201276 |
identifier_str_mv |
Macromolecular Research, v. 28, n. 2, p. 126-135, 2020. 2092-7673 1598-5032 10.1007/s13233-020-8019-y 2-s2.0-85074046943 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Macromolecular Research |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
126-135 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1803046760297267200 |