Loss of tumor-associated macrophages and vascular endothelial growth factor immunoexpression in solid mammary carcinoma in dogs
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.29261/pakvetj/2019.105 http://hdl.handle.net/11449/200136 |
Resumo: | Tumor-associated macrophages (TAM) are related to poor prognosis in canine mammary tumors (CMT). An association between TAM and sustained angiogenesis has been implicated with malignancy; however, comparison of the expression of TAM and vascular endothelial growth factor (VEGF) in different histologic types of CMT has not been completely studied. The objective of this study was to evaluate the immunostaining of TAM and VEGF in four different types of malignant CMT. Ninety-nine mammary carcinomas [tubular (29), tubulopapillary (22), mixed-type (28) and solid carcinoma (20)] were evaluated by immunohistochemical staining using MAC387 and VG-1 to detect TAM and VEGF immunoexpression, respectively. The total number of positive macrophages within the tumor was used for TAM immunolabeling and a score of four categories was used for VEGF immunolabeling. TAM immunoexpression was found to be statistically higher in tubular carcinomas than in solid carcinomas (P=0.0015). Differences between other types of carcinoma were not statistically significant. VEGF score was higher in tubular, papillary and mixed-type carcinomas than in solid carcinomas (P<0.0001). A positive relationship between the highest mean value of TAM and VEGF immunoexpression was also found (P=0.0015). TAM and VEGF expressions can be lost in more aggressive CMT, as in solid carcinoma. Furthermore, regardless of histologic type, higher numbers of TAM are associated with higher scores of VEGF, thus favoring the relationship between TAM and angiogenesis. |
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Loss of tumor-associated macrophages and vascular endothelial growth factor immunoexpression in solid mammary carcinoma in dogsImmunohistochemistryMammary tumorTAMVEGFTumor-associated macrophages (TAM) are related to poor prognosis in canine mammary tumors (CMT). An association between TAM and sustained angiogenesis has been implicated with malignancy; however, comparison of the expression of TAM and vascular endothelial growth factor (VEGF) in different histologic types of CMT has not been completely studied. The objective of this study was to evaluate the immunostaining of TAM and VEGF in four different types of malignant CMT. Ninety-nine mammary carcinomas [tubular (29), tubulopapillary (22), mixed-type (28) and solid carcinoma (20)] were evaluated by immunohistochemical staining using MAC387 and VG-1 to detect TAM and VEGF immunoexpression, respectively. The total number of positive macrophages within the tumor was used for TAM immunolabeling and a score of four categories was used for VEGF immunolabeling. TAM immunoexpression was found to be statistically higher in tubular carcinomas than in solid carcinomas (P=0.0015). Differences between other types of carcinoma were not statistically significant. VEGF score was higher in tubular, papillary and mixed-type carcinomas than in solid carcinomas (P<0.0001). A positive relationship between the highest mean value of TAM and VEGF immunoexpression was also found (P=0.0015). TAM and VEGF expressions can be lost in more aggressive CMT, as in solid carcinoma. Furthermore, regardless of histologic type, higher numbers of TAM are associated with higher scores of VEGF, thus favoring the relationship between TAM and angiogenesis.Clinic and Surgery Veterinary Department College of Agricultural and Veterinary Sciences São Paulo State University 'Júlio de Mesquita Filho' (UNESP), Via de Acesso Prof. Paulo Donato Castelane, S/N - Vila Industrial, Jaboticabal - SPCollege of Veterinary Medicine Franca University (UNIFRAN), Av. Dr. Armando de Sáles Oliveira, 201 - Parque UniversitarioPathology Department Federal Institute of Education Science and Technology of the South of Minas Gerais Campus of Muzambinho, Estrada de Muzambinho, km 35 - Bairro Morro PretoPathology Veterinary Department College of Veterinary and Zootechnical Medicine São Paulo State University 'Júlio de Mesquita Filho' (UNESP), Rua Prof. Doutor Walter Mauricio Correa, s/n.Clinic and Surgery Veterinary Department College of Agricultural and Veterinary Sciences São Paulo State University 'Júlio de Mesquita Filho' (UNESP), Via de Acesso Prof. Paulo Donato Castelane, S/N - Vila Industrial, Jaboticabal - SPPathology Veterinary Department College of Veterinary and Zootechnical Medicine São Paulo State University 'Júlio de Mesquita Filho' (UNESP), Rua Prof. Doutor Walter Mauricio Correa, s/n.Universidade Estadual Paulista (Unesp)Franca University (UNIFRAN)Science and Technology of the South of Minas GeraisMatiz, Oscar Rodrigo Sierra [UNESP]Santilli, Julianade Almeida, Maria Amélia MartinsMagalhães, Larissa FernandesMagalhães, Geórgia ModéAlves, Carlos Eduardo Fonseca [UNESP]de Nardi, Andrigo Barboza [UNESP]Calazans, Sabryna Gouveia [UNESP]2020-12-12T01:58:41Z2020-12-12T01:58:41Z2020-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article103-107http://dx.doi.org/10.29261/pakvetj/2019.105Pakistan Veterinary Journal, v. 40, n. 1, p. 103-107, 2020.2074-77640253-8318http://hdl.handle.net/11449/20013610.29261/pakvetj/2019.1052-s2.0-85081227283Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPakistan Veterinary Journalinfo:eu-repo/semantics/openAccess2024-06-06T14:10:21Zoai:repositorio.unesp.br:11449/200136Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-06-06T14:10:21Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Loss of tumor-associated macrophages and vascular endothelial growth factor immunoexpression in solid mammary carcinoma in dogs |
title |
Loss of tumor-associated macrophages and vascular endothelial growth factor immunoexpression in solid mammary carcinoma in dogs |
spellingShingle |
Loss of tumor-associated macrophages and vascular endothelial growth factor immunoexpression in solid mammary carcinoma in dogs Matiz, Oscar Rodrigo Sierra [UNESP] Immunohistochemistry Mammary tumor TAM VEGF |
title_short |
Loss of tumor-associated macrophages and vascular endothelial growth factor immunoexpression in solid mammary carcinoma in dogs |
title_full |
Loss of tumor-associated macrophages and vascular endothelial growth factor immunoexpression in solid mammary carcinoma in dogs |
title_fullStr |
Loss of tumor-associated macrophages and vascular endothelial growth factor immunoexpression in solid mammary carcinoma in dogs |
title_full_unstemmed |
Loss of tumor-associated macrophages and vascular endothelial growth factor immunoexpression in solid mammary carcinoma in dogs |
title_sort |
Loss of tumor-associated macrophages and vascular endothelial growth factor immunoexpression in solid mammary carcinoma in dogs |
author |
Matiz, Oscar Rodrigo Sierra [UNESP] |
author_facet |
Matiz, Oscar Rodrigo Sierra [UNESP] Santilli, Juliana de Almeida, Maria Amélia Martins Magalhães, Larissa Fernandes Magalhães, Geórgia Modé Alves, Carlos Eduardo Fonseca [UNESP] de Nardi, Andrigo Barboza [UNESP] Calazans, Sabryna Gouveia [UNESP] |
author_role |
author |
author2 |
Santilli, Juliana de Almeida, Maria Amélia Martins Magalhães, Larissa Fernandes Magalhães, Geórgia Modé Alves, Carlos Eduardo Fonseca [UNESP] de Nardi, Andrigo Barboza [UNESP] Calazans, Sabryna Gouveia [UNESP] |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Franca University (UNIFRAN) Science and Technology of the South of Minas Gerais |
dc.contributor.author.fl_str_mv |
Matiz, Oscar Rodrigo Sierra [UNESP] Santilli, Juliana de Almeida, Maria Amélia Martins Magalhães, Larissa Fernandes Magalhães, Geórgia Modé Alves, Carlos Eduardo Fonseca [UNESP] de Nardi, Andrigo Barboza [UNESP] Calazans, Sabryna Gouveia [UNESP] |
dc.subject.por.fl_str_mv |
Immunohistochemistry Mammary tumor TAM VEGF |
topic |
Immunohistochemistry Mammary tumor TAM VEGF |
description |
Tumor-associated macrophages (TAM) are related to poor prognosis in canine mammary tumors (CMT). An association between TAM and sustained angiogenesis has been implicated with malignancy; however, comparison of the expression of TAM and vascular endothelial growth factor (VEGF) in different histologic types of CMT has not been completely studied. The objective of this study was to evaluate the immunostaining of TAM and VEGF in four different types of malignant CMT. Ninety-nine mammary carcinomas [tubular (29), tubulopapillary (22), mixed-type (28) and solid carcinoma (20)] were evaluated by immunohistochemical staining using MAC387 and VG-1 to detect TAM and VEGF immunoexpression, respectively. The total number of positive macrophages within the tumor was used for TAM immunolabeling and a score of four categories was used for VEGF immunolabeling. TAM immunoexpression was found to be statistically higher in tubular carcinomas than in solid carcinomas (P=0.0015). Differences between other types of carcinoma were not statistically significant. VEGF score was higher in tubular, papillary and mixed-type carcinomas than in solid carcinomas (P<0.0001). A positive relationship between the highest mean value of TAM and VEGF immunoexpression was also found (P=0.0015). TAM and VEGF expressions can be lost in more aggressive CMT, as in solid carcinoma. Furthermore, regardless of histologic type, higher numbers of TAM are associated with higher scores of VEGF, thus favoring the relationship between TAM and angiogenesis. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-12T01:58:41Z 2020-12-12T01:58:41Z 2020-01-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.29261/pakvetj/2019.105 Pakistan Veterinary Journal, v. 40, n. 1, p. 103-107, 2020. 2074-7764 0253-8318 http://hdl.handle.net/11449/200136 10.29261/pakvetj/2019.105 2-s2.0-85081227283 |
url |
http://dx.doi.org/10.29261/pakvetj/2019.105 http://hdl.handle.net/11449/200136 |
identifier_str_mv |
Pakistan Veterinary Journal, v. 40, n. 1, p. 103-107, 2020. 2074-7764 0253-8318 10.29261/pakvetj/2019.105 2-s2.0-85081227283 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Pakistan Veterinary Journal |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
103-107 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1803045415660027904 |