Antimicrobial activity of psidium cattleianum associated with calcium hydroxide against enterococcus faecalis and candida albicans: An in vitro study
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1007/s00784-017-2326-5 http://hdl.handle.net/11449/175731 |
Resumo: | Objective Evaluate, in vitro, the antimicrobial activity of Psidium cattleianum leaf extracts combined with calcium hydroxide against Enterococcus faecalis and Candida albicans biofilm. Materials and methods Dentin specimens obtained from extracted bovine incisors were infected during 14 days with E. faecalis ATCC 29212 and C. albicans ATCC 10231. The specimens were filled with calcium hydroxide pastes prepared with the following vehicles: Psidium cattleianum ethanolic, Psidium cattleianum propylene glycolic, distilled water, and saline as control. After 24 h, 3, 7, and 14 days, the canals were irrigated with sterile saline and dried. Dentin samples were collected from the canals with burs of increasing diameters. To determine the number of colony-forming units (CFU), samples were inoculated onto BHI agar supplemented with yeast extract (0.5%), at 37 °C, for 48 h, in CO2 enriched atmosphere. Comparisons among the groups for the variation factors were performed by ANOVA and Tukey’s test. Results Ethanolic and propylene glycolic extracts showed significantly higher antimicrobial activity against E. faecalis (p <0.01) when compared with distilled water. The ethanolic extract exhibited in 24 h the same antibacterial activity that propylene glycolic extract and distilled water after 7 and 14 days. For C. albicans, all were effective in reducing the number of CFU at all periods. Conclusion The P. cattleianum ethanolic extract presented the fastest and highest antimicrobial activity against E. faecalis, significantly reducing the microbial load in 24 h. All medications were effective against C. albicans. Clinical relevance The antibacterial potential of P. cattleianum and its biological compatibility associated with calcium hydroxide indicate promising applications in the field of dentistry. |
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Antimicrobial activity of psidium cattleianum associated with calcium hydroxide against enterococcus faecalis and candida albicans: An in vitro studyCalcium hydroxideCandida albicansEnterococcus faecalisPlant extractsObjective Evaluate, in vitro, the antimicrobial activity of Psidium cattleianum leaf extracts combined with calcium hydroxide against Enterococcus faecalis and Candida albicans biofilm. Materials and methods Dentin specimens obtained from extracted bovine incisors were infected during 14 days with E. faecalis ATCC 29212 and C. albicans ATCC 10231. The specimens were filled with calcium hydroxide pastes prepared with the following vehicles: Psidium cattleianum ethanolic, Psidium cattleianum propylene glycolic, distilled water, and saline as control. After 24 h, 3, 7, and 14 days, the canals were irrigated with sterile saline and dried. Dentin samples were collected from the canals with burs of increasing diameters. To determine the number of colony-forming units (CFU), samples were inoculated onto BHI agar supplemented with yeast extract (0.5%), at 37 °C, for 48 h, in CO2 enriched atmosphere. Comparisons among the groups for the variation factors were performed by ANOVA and Tukey’s test. Results Ethanolic and propylene glycolic extracts showed significantly higher antimicrobial activity against E. faecalis (p <0.01) when compared with distilled water. The ethanolic extract exhibited in 24 h the same antibacterial activity that propylene glycolic extract and distilled water after 7 and 14 days. For C. albicans, all were effective in reducing the number of CFU at all periods. Conclusion The P. cattleianum ethanolic extract presented the fastest and highest antimicrobial activity against E. faecalis, significantly reducing the microbial load in 24 h. All medications were effective against C. albicans. Clinical relevance The antibacterial potential of P. cattleianum and its biological compatibility associated with calcium hydroxide indicate promising applications in the field of dentistry.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Department of Restorative Dentistry Endodontics Araçatuba School of Dentistry UNESP Univ Estadual Paulista, Rua José Bonifácio, 1193Department of Pathology Microbiology and Imunology Araçatuba School of Dentistry UNESP Univ Estadual PaulistaDepartment of Restorative Dentistry Endodontics Araçatuba School of Dentistry UNESP Univ Estadual Paulista, Rua José Bonifácio, 1193Department of Pathology Microbiology and Imunology Araçatuba School of Dentistry UNESP Univ Estadual PaulistaUniversidade Estadual Paulista (Unesp)Sangalli, Jorgiana [UNESP]Jardim Júnior, Elerson Gaetti [UNESP]Bueno, Carlos Roberto Emerenciano [UNESP]Jacinto, Rogério Castilho [UNESP]Sivieri-Araújo, Gustavo [UNESP]Filho, João Eduardo Gomes [UNESP]Cintra, Luciano Tavares Ângelo [UNESP]Dezan Junior, Eloi [UNESP]2018-12-11T17:17:16Z2018-12-11T17:17:16Z2018-07-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article2273-2279application/pdfhttp://dx.doi.org/10.1007/s00784-017-2326-5Clinical Oral Investigations, v. 22, n. 6, p. 2273-2279, 2018.1436-37711432-6981http://hdl.handle.net/11449/17573110.1007/s00784-017-2326-52-s2.0-850402406672-s2.0-85040240667.pdf881377727258197239536300770470170000-0002-5699-65780000-0002-2362-8920Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengClinical Oral Investigations0,9860,986info:eu-repo/semantics/openAccess2024-01-18T06:30:34Zoai:repositorio.unesp.br:11449/175731Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T23:22:11.398123Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Antimicrobial activity of psidium cattleianum associated with calcium hydroxide against enterococcus faecalis and candida albicans: An in vitro study |
title |
Antimicrobial activity of psidium cattleianum associated with calcium hydroxide against enterococcus faecalis and candida albicans: An in vitro study |
spellingShingle |
Antimicrobial activity of psidium cattleianum associated with calcium hydroxide against enterococcus faecalis and candida albicans: An in vitro study Sangalli, Jorgiana [UNESP] Calcium hydroxide Candida albicans Enterococcus faecalis Plant extracts |
title_short |
Antimicrobial activity of psidium cattleianum associated with calcium hydroxide against enterococcus faecalis and candida albicans: An in vitro study |
title_full |
Antimicrobial activity of psidium cattleianum associated with calcium hydroxide against enterococcus faecalis and candida albicans: An in vitro study |
title_fullStr |
Antimicrobial activity of psidium cattleianum associated with calcium hydroxide against enterococcus faecalis and candida albicans: An in vitro study |
title_full_unstemmed |
Antimicrobial activity of psidium cattleianum associated with calcium hydroxide against enterococcus faecalis and candida albicans: An in vitro study |
title_sort |
Antimicrobial activity of psidium cattleianum associated with calcium hydroxide against enterococcus faecalis and candida albicans: An in vitro study |
author |
Sangalli, Jorgiana [UNESP] |
author_facet |
Sangalli, Jorgiana [UNESP] Jardim Júnior, Elerson Gaetti [UNESP] Bueno, Carlos Roberto Emerenciano [UNESP] Jacinto, Rogério Castilho [UNESP] Sivieri-Araújo, Gustavo [UNESP] Filho, João Eduardo Gomes [UNESP] Cintra, Luciano Tavares Ângelo [UNESP] Dezan Junior, Eloi [UNESP] |
author_role |
author |
author2 |
Jardim Júnior, Elerson Gaetti [UNESP] Bueno, Carlos Roberto Emerenciano [UNESP] Jacinto, Rogério Castilho [UNESP] Sivieri-Araújo, Gustavo [UNESP] Filho, João Eduardo Gomes [UNESP] Cintra, Luciano Tavares Ângelo [UNESP] Dezan Junior, Eloi [UNESP] |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Sangalli, Jorgiana [UNESP] Jardim Júnior, Elerson Gaetti [UNESP] Bueno, Carlos Roberto Emerenciano [UNESP] Jacinto, Rogério Castilho [UNESP] Sivieri-Araújo, Gustavo [UNESP] Filho, João Eduardo Gomes [UNESP] Cintra, Luciano Tavares Ângelo [UNESP] Dezan Junior, Eloi [UNESP] |
dc.subject.por.fl_str_mv |
Calcium hydroxide Candida albicans Enterococcus faecalis Plant extracts |
topic |
Calcium hydroxide Candida albicans Enterococcus faecalis Plant extracts |
description |
Objective Evaluate, in vitro, the antimicrobial activity of Psidium cattleianum leaf extracts combined with calcium hydroxide against Enterococcus faecalis and Candida albicans biofilm. Materials and methods Dentin specimens obtained from extracted bovine incisors were infected during 14 days with E. faecalis ATCC 29212 and C. albicans ATCC 10231. The specimens were filled with calcium hydroxide pastes prepared with the following vehicles: Psidium cattleianum ethanolic, Psidium cattleianum propylene glycolic, distilled water, and saline as control. After 24 h, 3, 7, and 14 days, the canals were irrigated with sterile saline and dried. Dentin samples were collected from the canals with burs of increasing diameters. To determine the number of colony-forming units (CFU), samples were inoculated onto BHI agar supplemented with yeast extract (0.5%), at 37 °C, for 48 h, in CO2 enriched atmosphere. Comparisons among the groups for the variation factors were performed by ANOVA and Tukey’s test. Results Ethanolic and propylene glycolic extracts showed significantly higher antimicrobial activity against E. faecalis (p <0.01) when compared with distilled water. The ethanolic extract exhibited in 24 h the same antibacterial activity that propylene glycolic extract and distilled water after 7 and 14 days. For C. albicans, all were effective in reducing the number of CFU at all periods. Conclusion The P. cattleianum ethanolic extract presented the fastest and highest antimicrobial activity against E. faecalis, significantly reducing the microbial load in 24 h. All medications were effective against C. albicans. Clinical relevance The antibacterial potential of P. cattleianum and its biological compatibility associated with calcium hydroxide indicate promising applications in the field of dentistry. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-12-11T17:17:16Z 2018-12-11T17:17:16Z 2018-07-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/s00784-017-2326-5 Clinical Oral Investigations, v. 22, n. 6, p. 2273-2279, 2018. 1436-3771 1432-6981 http://hdl.handle.net/11449/175731 10.1007/s00784-017-2326-5 2-s2.0-85040240667 2-s2.0-85040240667.pdf 8813777272581972 3953630077047017 0000-0002-5699-6578 0000-0002-2362-8920 |
url |
http://dx.doi.org/10.1007/s00784-017-2326-5 http://hdl.handle.net/11449/175731 |
identifier_str_mv |
Clinical Oral Investigations, v. 22, n. 6, p. 2273-2279, 2018. 1436-3771 1432-6981 10.1007/s00784-017-2326-5 2-s2.0-85040240667 2-s2.0-85040240667.pdf 8813777272581972 3953630077047017 0000-0002-5699-6578 0000-0002-2362-8920 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Clinical Oral Investigations 0,986 0,986 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
2273-2279 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129512060223488 |