Prolactin induces maturation of glucose sensing mechanisms in cultured neonatal rat islets
Autor(a) principal: | |
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Data de Publicação: | 1993 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1210/endo.133.2.8344197 http://hdl.handle.net/11449/223961 |
Resumo: | The effects of PRL treatment on insulin content and secretion, and86Rb and45Ca fluxes from neonatal rat islets maintained in culture for 7-9 days were studied. PRL treatment enhanced islet insulin content by 40% and enhanced early insulin secretion evoked by 16.7 mM glucose. Insulin release stimulated by oxotremorine-M, a muscarinic agonist, in the presence of glucose (8.3 or 16.7 mM) was unchanged by PRL treatment. However, PRL treatment potentiated phorbol 12, 13-dibutyrate-stimulated insulin secretion in the presence of the above glucose concentrations. PRL treatment potentiated the reduction in86Rb efflux induced by glucose or tolbutamide and enhanced the increase in86Rb efflux evoked by diazoxide. PRL treatment slightly potentiated the increment in45Ca uptake induced by high concentrations of K+, but failed to affect the increment evoked by 16.7 mM glucose. Since glucose-induced45Ca uptake was not affected by PRL, we suggest that the enhancement in first phase insulin secretion evoked by glucose in the PRL-treated islets occurs at a step in the secretory process that may involve protein kinase-C. These data further support observations that PRL treatment increases islet sensitivity to glucose. © 1993 by The Endocrine Society. |
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Repositório Institucional da UNESP |
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Prolactin induces maturation of glucose sensing mechanisms in cultured neonatal rat isletsThe effects of PRL treatment on insulin content and secretion, and86Rb and45Ca fluxes from neonatal rat islets maintained in culture for 7-9 days were studied. PRL treatment enhanced islet insulin content by 40% and enhanced early insulin secretion evoked by 16.7 mM glucose. Insulin release stimulated by oxotremorine-M, a muscarinic agonist, in the presence of glucose (8.3 or 16.7 mM) was unchanged by PRL treatment. However, PRL treatment potentiated phorbol 12, 13-dibutyrate-stimulated insulin secretion in the presence of the above glucose concentrations. PRL treatment potentiated the reduction in86Rb efflux induced by glucose or tolbutamide and enhanced the increase in86Rb efflux evoked by diazoxide. PRL treatment slightly potentiated the increment in45Ca uptake induced by high concentrations of K+, but failed to affect the increment evoked by 16.7 mM glucose. Since glucose-induced45Ca uptake was not affected by PRL, we suggest that the enhancement in first phase insulin secretion evoked by glucose in the PRL-treated islets occurs at a step in the secretory process that may involve protein kinase-C. These data further support observations that PRL treatment increases islet sensitivity to glucose. © 1993 by The Endocrine Society.Laboratory of Cell Biology and Genetics National Institute of Diabetes and Digestive and Kidney Diseases National Institutes of Health, Bethesda, MD, 20892Departamento de Fisiologia e Biofisica Instituto de Biologia UNICAMP, Campinas, SP, 13081Departamento de Educacao Fisica Instituto de Biociencias UNESP, Rio Claro, SP, 13500Departamento de Educacao Fisica Instituto de Biociencias UNESP, Rio Claro, SP, 13500National Institutes of HealthUniversidade Estadual de Campinas (UNICAMP)Universidade Estadual Paulista (UNESP)Boschero, Antonio C.Crepaldi, Silvia C.Carneiro, Everardo M. [UNESP]Delattre, EdsonAtwater, Illani2022-04-28T19:53:57Z2022-04-28T19:53:57Z1993-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article515-520http://dx.doi.org/10.1210/endo.133.2.8344197Endocrinology, v. 133, n. 2, p. 515-520, 1993.1945-71700013-7227http://hdl.handle.net/11449/22396110.1210/endo.133.2.83441972-s2.0-0027296364Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengEndocrinologyinfo:eu-repo/semantics/openAccess2022-04-28T19:53:57Zoai:repositorio.unesp.br:11449/223961Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T21:50:11.102975Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Prolactin induces maturation of glucose sensing mechanisms in cultured neonatal rat islets |
title |
Prolactin induces maturation of glucose sensing mechanisms in cultured neonatal rat islets |
spellingShingle |
Prolactin induces maturation of glucose sensing mechanisms in cultured neonatal rat islets Boschero, Antonio C. |
title_short |
Prolactin induces maturation of glucose sensing mechanisms in cultured neonatal rat islets |
title_full |
Prolactin induces maturation of glucose sensing mechanisms in cultured neonatal rat islets |
title_fullStr |
Prolactin induces maturation of glucose sensing mechanisms in cultured neonatal rat islets |
title_full_unstemmed |
Prolactin induces maturation of glucose sensing mechanisms in cultured neonatal rat islets |
title_sort |
Prolactin induces maturation of glucose sensing mechanisms in cultured neonatal rat islets |
author |
Boschero, Antonio C. |
author_facet |
Boschero, Antonio C. Crepaldi, Silvia C. Carneiro, Everardo M. [UNESP] Delattre, Edson Atwater, Illani |
author_role |
author |
author2 |
Crepaldi, Silvia C. Carneiro, Everardo M. [UNESP] Delattre, Edson Atwater, Illani |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
National Institutes of Health Universidade Estadual de Campinas (UNICAMP) Universidade Estadual Paulista (UNESP) |
dc.contributor.author.fl_str_mv |
Boschero, Antonio C. Crepaldi, Silvia C. Carneiro, Everardo M. [UNESP] Delattre, Edson Atwater, Illani |
description |
The effects of PRL treatment on insulin content and secretion, and86Rb and45Ca fluxes from neonatal rat islets maintained in culture for 7-9 days were studied. PRL treatment enhanced islet insulin content by 40% and enhanced early insulin secretion evoked by 16.7 mM glucose. Insulin release stimulated by oxotremorine-M, a muscarinic agonist, in the presence of glucose (8.3 or 16.7 mM) was unchanged by PRL treatment. However, PRL treatment potentiated phorbol 12, 13-dibutyrate-stimulated insulin secretion in the presence of the above glucose concentrations. PRL treatment potentiated the reduction in86Rb efflux induced by glucose or tolbutamide and enhanced the increase in86Rb efflux evoked by diazoxide. PRL treatment slightly potentiated the increment in45Ca uptake induced by high concentrations of K+, but failed to affect the increment evoked by 16.7 mM glucose. Since glucose-induced45Ca uptake was not affected by PRL, we suggest that the enhancement in first phase insulin secretion evoked by glucose in the PRL-treated islets occurs at a step in the secretory process that may involve protein kinase-C. These data further support observations that PRL treatment increases islet sensitivity to glucose. © 1993 by The Endocrine Society. |
publishDate |
1993 |
dc.date.none.fl_str_mv |
1993-01-01 2022-04-28T19:53:57Z 2022-04-28T19:53:57Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1210/endo.133.2.8344197 Endocrinology, v. 133, n. 2, p. 515-520, 1993. 1945-7170 0013-7227 http://hdl.handle.net/11449/223961 10.1210/endo.133.2.8344197 2-s2.0-0027296364 |
url |
http://dx.doi.org/10.1210/endo.133.2.8344197 http://hdl.handle.net/11449/223961 |
identifier_str_mv |
Endocrinology, v. 133, n. 2, p. 515-520, 1993. 1945-7170 0013-7227 10.1210/endo.133.2.8344197 2-s2.0-0027296364 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Endocrinology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
515-520 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129364516143104 |