Anxiogenic and antinociceptive effects induced by corticotropin-releasing factor (CRF) injections into the periaqueductal gray are modulated by CRF1 receptor in mice

Detalhes bibliográficos
Autor(a) principal: Miguel, Tarciso Tadeu [UNESP]
Data de Publicação: 2011
Outros Autores: Nunes-de-Souza, Ricardo Luiz [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.yhbeh.2011.06.004
http://hdl.handle.net/11449/42597
Resumo: Chemical or electrical stimulation of the dorsal portion of the midbrain periaqueductal gray (dPAG) produces anxiogenic and antinociceptive effects. In rats, chemical stimulation of dPAG by local infusion of the neuropeptide corticotropin-releasing factor (CRF) provokes anxiogenic effects in the elevated plus-maze test (EPM). CRF also produces antinociception when injected intracerebroventricularly in rats, however it remains unclear whether this response is also observed following CRF injection into the dPAG in mice. Yet, given that there are CRF1 and CRF2 receptor subtypes within the PAG, it is important to show in which receptor subtypes CRF exert its anxiogenic and antinociceptive effects in the dPAG. Here, we investigated the role of these receptors in the anxiogenic (assessed in the EPM) and antinociceptive (assessed by the Formalin test: 2.5% formalin injection into the right hind paw) effects following intra-dPAG infusion of CRF in mice. The results show that intra-dPAG injections of CRF (75 pmol/0.1 mu l and 150 pmol/0.2 mu l) produced dose-dependent anxiogenic and antinociceptive effects. In addition, local infusion of NBI 27914 (5-chloro-4-(N-(cyclopropyl) methyl-N-propylamino)-2-methyl-6-(2,4,6-trichlorophenyl)-aminopyridine; 2 nmo1/0.2 pl), a CRF1 receptor antagonist, completely blocked both the anxiogenic and antinociceptive effects induced by local infusion of CRF, while that of antisauvagine 30 (ASV30; 1 nmol/0.2 mu l), a CRF2 receptor antagonist, did not alter the CRF effects. Present results are suggestive that CRF1 (but not CRF2) receptors play a crucial role in the anxiogenic and antinociceptive effects induced by CRF in the dPAG in mice. (C) 2011 Elsevier B.V. All rights reserved.
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spelling Anxiogenic and antinociceptive effects induced by corticotropin-releasing factor (CRF) injections into the periaqueductal gray are modulated by CRF1 receptor in miceAnxietyAntinociceptionCRF1 and CRF2 receptorsPeriaqueductal grayElevated plus-mazeMiceChemical or electrical stimulation of the dorsal portion of the midbrain periaqueductal gray (dPAG) produces anxiogenic and antinociceptive effects. In rats, chemical stimulation of dPAG by local infusion of the neuropeptide corticotropin-releasing factor (CRF) provokes anxiogenic effects in the elevated plus-maze test (EPM). CRF also produces antinociception when injected intracerebroventricularly in rats, however it remains unclear whether this response is also observed following CRF injection into the dPAG in mice. Yet, given that there are CRF1 and CRF2 receptor subtypes within the PAG, it is important to show in which receptor subtypes CRF exert its anxiogenic and antinociceptive effects in the dPAG. Here, we investigated the role of these receptors in the anxiogenic (assessed in the EPM) and antinociceptive (assessed by the Formalin test: 2.5% formalin injection into the right hind paw) effects following intra-dPAG infusion of CRF in mice. The results show that intra-dPAG injections of CRF (75 pmol/0.1 mu l and 150 pmol/0.2 mu l) produced dose-dependent anxiogenic and antinociceptive effects. In addition, local infusion of NBI 27914 (5-chloro-4-(N-(cyclopropyl) methyl-N-propylamino)-2-methyl-6-(2,4,6-trichlorophenyl)-aminopyridine; 2 nmo1/0.2 pl), a CRF1 receptor antagonist, completely blocked both the anxiogenic and antinociceptive effects induced by local infusion of CRF, while that of antisauvagine 30 (ASV30; 1 nmol/0.2 mu l), a CRF2 receptor antagonist, did not alter the CRF effects. Present results are suggestive that CRF1 (but not CRF2) receptors play a crucial role in the anxiogenic and antinociceptive effects induced by CRF in the dPAG in mice. (C) 2011 Elsevier B.V. All rights reserved.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Programa de Apoio ao Desenvolvimento Científico da Faculdade de Ciências Farmacêuticas da UNESP (PADC)UNESP, Fac Ciencias Farmaceut, Farmacol Lab, BR-14801902 Araraquara, SP, BrazilUniversidade Federal de São Carlos (UFSCar), Programa Interinst Posgrad Ciencias Fisiol, BR-14801902 Araraquara, SP, BrazilUniv Estadual Paulista, BR-14801902 Araraquara, SP, BrazilUNESP, Fac Ciencias Farmaceut, Farmacol Lab, BR-14801902 Araraquara, SP, BrazilUniv Estadual Paulista, BR-14801902 Araraquara, SP, BrazilFAPESP: 05/05171-1CNPq: 303580/2009-7Academic Press Inc. Elsevier B.V.Universidade Estadual Paulista (Unesp)Universidade Federal de São Carlos (UFSCar)Miguel, Tarciso Tadeu [UNESP]Nunes-de-Souza, Ricardo Luiz [UNESP]2014-05-20T15:34:36Z2014-05-20T15:34:36Z2011-08-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article292-300application/pdfhttp://dx.doi.org/10.1016/j.yhbeh.2011.06.004Hormones and Behavior. San Diego: Academic Press Inc. Elsevier B.V., v. 60, n. 3, p. 292-300, 2011.0018-506Xhttp://hdl.handle.net/11449/4259710.1016/j.yhbeh.2011.06.004WOS:000293316000010WOS000293316000010.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengHormones and Behavior4.418info:eu-repo/semantics/openAccess2024-06-24T14:51:41Zoai:repositorio.unesp.br:11449/42597Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T17:21:34.850054Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Anxiogenic and antinociceptive effects induced by corticotropin-releasing factor (CRF) injections into the periaqueductal gray are modulated by CRF1 receptor in mice
title Anxiogenic and antinociceptive effects induced by corticotropin-releasing factor (CRF) injections into the periaqueductal gray are modulated by CRF1 receptor in mice
spellingShingle Anxiogenic and antinociceptive effects induced by corticotropin-releasing factor (CRF) injections into the periaqueductal gray are modulated by CRF1 receptor in mice
Miguel, Tarciso Tadeu [UNESP]
Anxiety
Antinociception
CRF1 and CRF2 receptors
Periaqueductal gray
Elevated plus-maze
Mice
title_short Anxiogenic and antinociceptive effects induced by corticotropin-releasing factor (CRF) injections into the periaqueductal gray are modulated by CRF1 receptor in mice
title_full Anxiogenic and antinociceptive effects induced by corticotropin-releasing factor (CRF) injections into the periaqueductal gray are modulated by CRF1 receptor in mice
title_fullStr Anxiogenic and antinociceptive effects induced by corticotropin-releasing factor (CRF) injections into the periaqueductal gray are modulated by CRF1 receptor in mice
title_full_unstemmed Anxiogenic and antinociceptive effects induced by corticotropin-releasing factor (CRF) injections into the periaqueductal gray are modulated by CRF1 receptor in mice
title_sort Anxiogenic and antinociceptive effects induced by corticotropin-releasing factor (CRF) injections into the periaqueductal gray are modulated by CRF1 receptor in mice
author Miguel, Tarciso Tadeu [UNESP]
author_facet Miguel, Tarciso Tadeu [UNESP]
Nunes-de-Souza, Ricardo Luiz [UNESP]
author_role author
author2 Nunes-de-Souza, Ricardo Luiz [UNESP]
author2_role author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Universidade Federal de São Carlos (UFSCar)
dc.contributor.author.fl_str_mv Miguel, Tarciso Tadeu [UNESP]
Nunes-de-Souza, Ricardo Luiz [UNESP]
dc.subject.por.fl_str_mv Anxiety
Antinociception
CRF1 and CRF2 receptors
Periaqueductal gray
Elevated plus-maze
Mice
topic Anxiety
Antinociception
CRF1 and CRF2 receptors
Periaqueductal gray
Elevated plus-maze
Mice
description Chemical or electrical stimulation of the dorsal portion of the midbrain periaqueductal gray (dPAG) produces anxiogenic and antinociceptive effects. In rats, chemical stimulation of dPAG by local infusion of the neuropeptide corticotropin-releasing factor (CRF) provokes anxiogenic effects in the elevated plus-maze test (EPM). CRF also produces antinociception when injected intracerebroventricularly in rats, however it remains unclear whether this response is also observed following CRF injection into the dPAG in mice. Yet, given that there are CRF1 and CRF2 receptor subtypes within the PAG, it is important to show in which receptor subtypes CRF exert its anxiogenic and antinociceptive effects in the dPAG. Here, we investigated the role of these receptors in the anxiogenic (assessed in the EPM) and antinociceptive (assessed by the Formalin test: 2.5% formalin injection into the right hind paw) effects following intra-dPAG infusion of CRF in mice. The results show that intra-dPAG injections of CRF (75 pmol/0.1 mu l and 150 pmol/0.2 mu l) produced dose-dependent anxiogenic and antinociceptive effects. In addition, local infusion of NBI 27914 (5-chloro-4-(N-(cyclopropyl) methyl-N-propylamino)-2-methyl-6-(2,4,6-trichlorophenyl)-aminopyridine; 2 nmo1/0.2 pl), a CRF1 receptor antagonist, completely blocked both the anxiogenic and antinociceptive effects induced by local infusion of CRF, while that of antisauvagine 30 (ASV30; 1 nmol/0.2 mu l), a CRF2 receptor antagonist, did not alter the CRF effects. Present results are suggestive that CRF1 (but not CRF2) receptors play a crucial role in the anxiogenic and antinociceptive effects induced by CRF in the dPAG in mice. (C) 2011 Elsevier B.V. All rights reserved.
publishDate 2011
dc.date.none.fl_str_mv 2011-08-01
2014-05-20T15:34:36Z
2014-05-20T15:34:36Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.yhbeh.2011.06.004
Hormones and Behavior. San Diego: Academic Press Inc. Elsevier B.V., v. 60, n. 3, p. 292-300, 2011.
0018-506X
http://hdl.handle.net/11449/42597
10.1016/j.yhbeh.2011.06.004
WOS:000293316000010
WOS000293316000010.pdf
url http://dx.doi.org/10.1016/j.yhbeh.2011.06.004
http://hdl.handle.net/11449/42597
identifier_str_mv Hormones and Behavior. San Diego: Academic Press Inc. Elsevier B.V., v. 60, n. 3, p. 292-300, 2011.
0018-506X
10.1016/j.yhbeh.2011.06.004
WOS:000293316000010
WOS000293316000010.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Hormones and Behavior
4.418
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 292-300
application/pdf
dc.publisher.none.fl_str_mv Academic Press Inc. Elsevier B.V.
publisher.none.fl_str_mv Academic Press Inc. Elsevier B.V.
dc.source.none.fl_str_mv Web of Science
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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