Anxiogenic and antinociceptive effects induced by corticotropin-releasing factor (CRF) injections into the periaqueductal gray are modulated by CRF1 receptor in mice
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.yhbeh.2011.06.004 http://hdl.handle.net/11449/42597 |
Resumo: | Chemical or electrical stimulation of the dorsal portion of the midbrain periaqueductal gray (dPAG) produces anxiogenic and antinociceptive effects. In rats, chemical stimulation of dPAG by local infusion of the neuropeptide corticotropin-releasing factor (CRF) provokes anxiogenic effects in the elevated plus-maze test (EPM). CRF also produces antinociception when injected intracerebroventricularly in rats, however it remains unclear whether this response is also observed following CRF injection into the dPAG in mice. Yet, given that there are CRF1 and CRF2 receptor subtypes within the PAG, it is important to show in which receptor subtypes CRF exert its anxiogenic and antinociceptive effects in the dPAG. Here, we investigated the role of these receptors in the anxiogenic (assessed in the EPM) and antinociceptive (assessed by the Formalin test: 2.5% formalin injection into the right hind paw) effects following intra-dPAG infusion of CRF in mice. The results show that intra-dPAG injections of CRF (75 pmol/0.1 mu l and 150 pmol/0.2 mu l) produced dose-dependent anxiogenic and antinociceptive effects. In addition, local infusion of NBI 27914 (5-chloro-4-(N-(cyclopropyl) methyl-N-propylamino)-2-methyl-6-(2,4,6-trichlorophenyl)-aminopyridine; 2 nmo1/0.2 pl), a CRF1 receptor antagonist, completely blocked both the anxiogenic and antinociceptive effects induced by local infusion of CRF, while that of antisauvagine 30 (ASV30; 1 nmol/0.2 mu l), a CRF2 receptor antagonist, did not alter the CRF effects. Present results are suggestive that CRF1 (but not CRF2) receptors play a crucial role in the anxiogenic and antinociceptive effects induced by CRF in the dPAG in mice. (C) 2011 Elsevier B.V. All rights reserved. |
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Anxiogenic and antinociceptive effects induced by corticotropin-releasing factor (CRF) injections into the periaqueductal gray are modulated by CRF1 receptor in miceAnxietyAntinociceptionCRF1 and CRF2 receptorsPeriaqueductal grayElevated plus-mazeMiceChemical or electrical stimulation of the dorsal portion of the midbrain periaqueductal gray (dPAG) produces anxiogenic and antinociceptive effects. In rats, chemical stimulation of dPAG by local infusion of the neuropeptide corticotropin-releasing factor (CRF) provokes anxiogenic effects in the elevated plus-maze test (EPM). CRF also produces antinociception when injected intracerebroventricularly in rats, however it remains unclear whether this response is also observed following CRF injection into the dPAG in mice. Yet, given that there are CRF1 and CRF2 receptor subtypes within the PAG, it is important to show in which receptor subtypes CRF exert its anxiogenic and antinociceptive effects in the dPAG. Here, we investigated the role of these receptors in the anxiogenic (assessed in the EPM) and antinociceptive (assessed by the Formalin test: 2.5% formalin injection into the right hind paw) effects following intra-dPAG infusion of CRF in mice. The results show that intra-dPAG injections of CRF (75 pmol/0.1 mu l and 150 pmol/0.2 mu l) produced dose-dependent anxiogenic and antinociceptive effects. In addition, local infusion of NBI 27914 (5-chloro-4-(N-(cyclopropyl) methyl-N-propylamino)-2-methyl-6-(2,4,6-trichlorophenyl)-aminopyridine; 2 nmo1/0.2 pl), a CRF1 receptor antagonist, completely blocked both the anxiogenic and antinociceptive effects induced by local infusion of CRF, while that of antisauvagine 30 (ASV30; 1 nmol/0.2 mu l), a CRF2 receptor antagonist, did not alter the CRF effects. Present results are suggestive that CRF1 (but not CRF2) receptors play a crucial role in the anxiogenic and antinociceptive effects induced by CRF in the dPAG in mice. (C) 2011 Elsevier B.V. All rights reserved.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Programa de Apoio ao Desenvolvimento Científico da Faculdade de Ciências Farmacêuticas da UNESP (PADC)UNESP, Fac Ciencias Farmaceut, Farmacol Lab, BR-14801902 Araraquara, SP, BrazilUniversidade Federal de São Carlos (UFSCar), Programa Interinst Posgrad Ciencias Fisiol, BR-14801902 Araraquara, SP, BrazilUniv Estadual Paulista, BR-14801902 Araraquara, SP, BrazilUNESP, Fac Ciencias Farmaceut, Farmacol Lab, BR-14801902 Araraquara, SP, BrazilUniv Estadual Paulista, BR-14801902 Araraquara, SP, BrazilFAPESP: 05/05171-1CNPq: 303580/2009-7Academic Press Inc. Elsevier B.V.Universidade Estadual Paulista (Unesp)Universidade Federal de São Carlos (UFSCar)Miguel, Tarciso Tadeu [UNESP]Nunes-de-Souza, Ricardo Luiz [UNESP]2014-05-20T15:34:36Z2014-05-20T15:34:36Z2011-08-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article292-300application/pdfhttp://dx.doi.org/10.1016/j.yhbeh.2011.06.004Hormones and Behavior. San Diego: Academic Press Inc. Elsevier B.V., v. 60, n. 3, p. 292-300, 2011.0018-506Xhttp://hdl.handle.net/11449/4259710.1016/j.yhbeh.2011.06.004WOS:000293316000010WOS000293316000010.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengHormones and Behavior4.418info:eu-repo/semantics/openAccess2024-06-24T14:51:41Zoai:repositorio.unesp.br:11449/42597Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T17:21:34.850054Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Anxiogenic and antinociceptive effects induced by corticotropin-releasing factor (CRF) injections into the periaqueductal gray are modulated by CRF1 receptor in mice |
title |
Anxiogenic and antinociceptive effects induced by corticotropin-releasing factor (CRF) injections into the periaqueductal gray are modulated by CRF1 receptor in mice |
spellingShingle |
Anxiogenic and antinociceptive effects induced by corticotropin-releasing factor (CRF) injections into the periaqueductal gray are modulated by CRF1 receptor in mice Miguel, Tarciso Tadeu [UNESP] Anxiety Antinociception CRF1 and CRF2 receptors Periaqueductal gray Elevated plus-maze Mice |
title_short |
Anxiogenic and antinociceptive effects induced by corticotropin-releasing factor (CRF) injections into the periaqueductal gray are modulated by CRF1 receptor in mice |
title_full |
Anxiogenic and antinociceptive effects induced by corticotropin-releasing factor (CRF) injections into the periaqueductal gray are modulated by CRF1 receptor in mice |
title_fullStr |
Anxiogenic and antinociceptive effects induced by corticotropin-releasing factor (CRF) injections into the periaqueductal gray are modulated by CRF1 receptor in mice |
title_full_unstemmed |
Anxiogenic and antinociceptive effects induced by corticotropin-releasing factor (CRF) injections into the periaqueductal gray are modulated by CRF1 receptor in mice |
title_sort |
Anxiogenic and antinociceptive effects induced by corticotropin-releasing factor (CRF) injections into the periaqueductal gray are modulated by CRF1 receptor in mice |
author |
Miguel, Tarciso Tadeu [UNESP] |
author_facet |
Miguel, Tarciso Tadeu [UNESP] Nunes-de-Souza, Ricardo Luiz [UNESP] |
author_role |
author |
author2 |
Nunes-de-Souza, Ricardo Luiz [UNESP] |
author2_role |
author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade Federal de São Carlos (UFSCar) |
dc.contributor.author.fl_str_mv |
Miguel, Tarciso Tadeu [UNESP] Nunes-de-Souza, Ricardo Luiz [UNESP] |
dc.subject.por.fl_str_mv |
Anxiety Antinociception CRF1 and CRF2 receptors Periaqueductal gray Elevated plus-maze Mice |
topic |
Anxiety Antinociception CRF1 and CRF2 receptors Periaqueductal gray Elevated plus-maze Mice |
description |
Chemical or electrical stimulation of the dorsal portion of the midbrain periaqueductal gray (dPAG) produces anxiogenic and antinociceptive effects. In rats, chemical stimulation of dPAG by local infusion of the neuropeptide corticotropin-releasing factor (CRF) provokes anxiogenic effects in the elevated plus-maze test (EPM). CRF also produces antinociception when injected intracerebroventricularly in rats, however it remains unclear whether this response is also observed following CRF injection into the dPAG in mice. Yet, given that there are CRF1 and CRF2 receptor subtypes within the PAG, it is important to show in which receptor subtypes CRF exert its anxiogenic and antinociceptive effects in the dPAG. Here, we investigated the role of these receptors in the anxiogenic (assessed in the EPM) and antinociceptive (assessed by the Formalin test: 2.5% formalin injection into the right hind paw) effects following intra-dPAG infusion of CRF in mice. The results show that intra-dPAG injections of CRF (75 pmol/0.1 mu l and 150 pmol/0.2 mu l) produced dose-dependent anxiogenic and antinociceptive effects. In addition, local infusion of NBI 27914 (5-chloro-4-(N-(cyclopropyl) methyl-N-propylamino)-2-methyl-6-(2,4,6-trichlorophenyl)-aminopyridine; 2 nmo1/0.2 pl), a CRF1 receptor antagonist, completely blocked both the anxiogenic and antinociceptive effects induced by local infusion of CRF, while that of antisauvagine 30 (ASV30; 1 nmol/0.2 mu l), a CRF2 receptor antagonist, did not alter the CRF effects. Present results are suggestive that CRF1 (but not CRF2) receptors play a crucial role in the anxiogenic and antinociceptive effects induced by CRF in the dPAG in mice. (C) 2011 Elsevier B.V. All rights reserved. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-08-01 2014-05-20T15:34:36Z 2014-05-20T15:34:36Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.yhbeh.2011.06.004 Hormones and Behavior. San Diego: Academic Press Inc. Elsevier B.V., v. 60, n. 3, p. 292-300, 2011. 0018-506X http://hdl.handle.net/11449/42597 10.1016/j.yhbeh.2011.06.004 WOS:000293316000010 WOS000293316000010.pdf |
url |
http://dx.doi.org/10.1016/j.yhbeh.2011.06.004 http://hdl.handle.net/11449/42597 |
identifier_str_mv |
Hormones and Behavior. San Diego: Academic Press Inc. Elsevier B.V., v. 60, n. 3, p. 292-300, 2011. 0018-506X 10.1016/j.yhbeh.2011.06.004 WOS:000293316000010 WOS000293316000010.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Hormones and Behavior 4.418 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
292-300 application/pdf |
dc.publisher.none.fl_str_mv |
Academic Press Inc. Elsevier B.V. |
publisher.none.fl_str_mv |
Academic Press Inc. Elsevier B.V. |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128797759766528 |