Influence of chronic alcoholism and estrogen deficiency on the immunohistochemical expression of regulatory proteins of the bone resorption process in the periodontium of Wistar rats

Detalhes bibliográficos
Autor(a) principal: Marchini, Adriana Mathias Pereira de Silva [UNESP]
Data de Publicação: 2018
Outros Autores: Santana-Melo, Gabriela de Fátima [UNESP], Salgado, Miriane Carneiro Machado [UNESP], Zutin, Elis Andrade de Lima [UNESP], Marchini, Leonardo, Rocha, Rosilene Fernandes da [UNESP], Vasconcellos, Luana Marotta Reis de [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.archoralbio.2018.07.006
http://hdl.handle.net/11449/180032
Resumo: Objective: The aim of this study was to investigate possible changes in immunohistochemical expression of proteins regulating the bone resorption process in the periodontium of rats subjected to alcoholism and/or estrogen deficiency. The investigated proteins were receptor activator of nuclear factor-kappa β ligand (RANKL), a protein that stimulates bone resorption, and osteoprotegerin (OPG), a protein that inhibits bone resorption. At the molecular level, decreased OPG expression and/or increased RANKL expression are consistent with a greater predisposition to bone resorption. Design: Wistar female rats were divided into ovariectomized (ovx) and non-ovariectomized (sham) groups, and subdivided into ad libitum diet (free diet), alcoholic diet (20% solution), and isocaloric diet (diet with a similar amount of calories as compared with groups ingesting an alcoholic diet). The alveolar bone crest and adjacent tissues were evaluated by immunohistochemical analyses for detection of OPG and RANKL. Results: A significant decrease in OPG expression and a significant increase in RANKL expression were observed in ovariectomized animals which received alcohol as compared with non-ovariectomized animals which received isocaloric diet (experimental control). When estrogen deficiency was evaluated independently of the diet type, a significant decrease in OPG expression and a significant increase in RANKL expression were observed in ovariectomized animals as compared with non-ovariectomized animals. Conclusions: Estrogen deficiency associated with alcoholic diet, as well as estrogen deficiency (analyzed independently of diet type), decreased the immunostaining for OPG and increased the immunostaining for RANKL in the periodontium of rats.
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spelling Influence of chronic alcoholism and estrogen deficiency on the immunohistochemical expression of regulatory proteins of the bone resorption process in the periodontium of Wistar ratsAlcoholismOsteoporosisOvariectomyPeriodontiumObjective: The aim of this study was to investigate possible changes in immunohistochemical expression of proteins regulating the bone resorption process in the periodontium of rats subjected to alcoholism and/or estrogen deficiency. The investigated proteins were receptor activator of nuclear factor-kappa β ligand (RANKL), a protein that stimulates bone resorption, and osteoprotegerin (OPG), a protein that inhibits bone resorption. At the molecular level, decreased OPG expression and/or increased RANKL expression are consistent with a greater predisposition to bone resorption. Design: Wistar female rats were divided into ovariectomized (ovx) and non-ovariectomized (sham) groups, and subdivided into ad libitum diet (free diet), alcoholic diet (20% solution), and isocaloric diet (diet with a similar amount of calories as compared with groups ingesting an alcoholic diet). The alveolar bone crest and adjacent tissues were evaluated by immunohistochemical analyses for detection of OPG and RANKL. Results: A significant decrease in OPG expression and a significant increase in RANKL expression were observed in ovariectomized animals which received alcohol as compared with non-ovariectomized animals which received isocaloric diet (experimental control). When estrogen deficiency was evaluated independently of the diet type, a significant decrease in OPG expression and a significant increase in RANKL expression were observed in ovariectomized animals as compared with non-ovariectomized animals. Conclusions: Estrogen deficiency associated with alcoholic diet, as well as estrogen deficiency (analyzed independently of diet type), decreased the immunostaining for OPG and increased the immunostaining for RANKL in the periodontium of rats.Department of Biosciences and Diagnostics Institute of Science and Technology UNESP - Univ Estadual Paulista, Av. Engenheiro Francisco José Longo, 777, São José dos CamposDepartment of Preventive and Community Dentistry The University of Iowa College of Dentistry and Dental Clinics, 801 Newton RoadDepartment of Biosciences and Diagnostics Institute of Science and Technology UNESP - Univ Estadual Paulista, Av. Engenheiro Francisco José Longo, 777, São José dos CamposUniversidade Estadual Paulista (Unesp)The University of Iowa College of Dentistry and Dental ClinicsMarchini, Adriana Mathias Pereira de Silva [UNESP]Santana-Melo, Gabriela de Fátima [UNESP]Salgado, Miriane Carneiro Machado [UNESP]Zutin, Elis Andrade de Lima [UNESP]Marchini, LeonardoRocha, Rosilene Fernandes da [UNESP]Vasconcellos, Luana Marotta Reis de [UNESP]2018-12-11T17:37:44Z2018-12-11T17:37:44Z2018-11-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article7-14application/pdfhttp://dx.doi.org/10.1016/j.archoralbio.2018.07.006Archives of Oral Biology, v. 95, p. 7-14.1879-15060003-9969http://hdl.handle.net/11449/18003210.1016/j.archoralbio.2018.07.0062-s2.0-850499856582-s2.0-85049985658.pdf5006685136114327Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengArchives of Oral Biology0,752info:eu-repo/semantics/openAccess2024-01-26T06:30:26Zoai:repositorio.unesp.br:11449/180032Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-06T00:00:52.485541Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Influence of chronic alcoholism and estrogen deficiency on the immunohistochemical expression of regulatory proteins of the bone resorption process in the periodontium of Wistar rats
title Influence of chronic alcoholism and estrogen deficiency on the immunohistochemical expression of regulatory proteins of the bone resorption process in the periodontium of Wistar rats
spellingShingle Influence of chronic alcoholism and estrogen deficiency on the immunohistochemical expression of regulatory proteins of the bone resorption process in the periodontium of Wistar rats
Marchini, Adriana Mathias Pereira de Silva [UNESP]
Alcoholism
Osteoporosis
Ovariectomy
Periodontium
title_short Influence of chronic alcoholism and estrogen deficiency on the immunohistochemical expression of regulatory proteins of the bone resorption process in the periodontium of Wistar rats
title_full Influence of chronic alcoholism and estrogen deficiency on the immunohistochemical expression of regulatory proteins of the bone resorption process in the periodontium of Wistar rats
title_fullStr Influence of chronic alcoholism and estrogen deficiency on the immunohistochemical expression of regulatory proteins of the bone resorption process in the periodontium of Wistar rats
title_full_unstemmed Influence of chronic alcoholism and estrogen deficiency on the immunohistochemical expression of regulatory proteins of the bone resorption process in the periodontium of Wistar rats
title_sort Influence of chronic alcoholism and estrogen deficiency on the immunohistochemical expression of regulatory proteins of the bone resorption process in the periodontium of Wistar rats
author Marchini, Adriana Mathias Pereira de Silva [UNESP]
author_facet Marchini, Adriana Mathias Pereira de Silva [UNESP]
Santana-Melo, Gabriela de Fátima [UNESP]
Salgado, Miriane Carneiro Machado [UNESP]
Zutin, Elis Andrade de Lima [UNESP]
Marchini, Leonardo
Rocha, Rosilene Fernandes da [UNESP]
Vasconcellos, Luana Marotta Reis de [UNESP]
author_role author
author2 Santana-Melo, Gabriela de Fátima [UNESP]
Salgado, Miriane Carneiro Machado [UNESP]
Zutin, Elis Andrade de Lima [UNESP]
Marchini, Leonardo
Rocha, Rosilene Fernandes da [UNESP]
Vasconcellos, Luana Marotta Reis de [UNESP]
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
The University of Iowa College of Dentistry and Dental Clinics
dc.contributor.author.fl_str_mv Marchini, Adriana Mathias Pereira de Silva [UNESP]
Santana-Melo, Gabriela de Fátima [UNESP]
Salgado, Miriane Carneiro Machado [UNESP]
Zutin, Elis Andrade de Lima [UNESP]
Marchini, Leonardo
Rocha, Rosilene Fernandes da [UNESP]
Vasconcellos, Luana Marotta Reis de [UNESP]
dc.subject.por.fl_str_mv Alcoholism
Osteoporosis
Ovariectomy
Periodontium
topic Alcoholism
Osteoporosis
Ovariectomy
Periodontium
description Objective: The aim of this study was to investigate possible changes in immunohistochemical expression of proteins regulating the bone resorption process in the periodontium of rats subjected to alcoholism and/or estrogen deficiency. The investigated proteins were receptor activator of nuclear factor-kappa β ligand (RANKL), a protein that stimulates bone resorption, and osteoprotegerin (OPG), a protein that inhibits bone resorption. At the molecular level, decreased OPG expression and/or increased RANKL expression are consistent with a greater predisposition to bone resorption. Design: Wistar female rats were divided into ovariectomized (ovx) and non-ovariectomized (sham) groups, and subdivided into ad libitum diet (free diet), alcoholic diet (20% solution), and isocaloric diet (diet with a similar amount of calories as compared with groups ingesting an alcoholic diet). The alveolar bone crest and adjacent tissues were evaluated by immunohistochemical analyses for detection of OPG and RANKL. Results: A significant decrease in OPG expression and a significant increase in RANKL expression were observed in ovariectomized animals which received alcohol as compared with non-ovariectomized animals which received isocaloric diet (experimental control). When estrogen deficiency was evaluated independently of the diet type, a significant decrease in OPG expression and a significant increase in RANKL expression were observed in ovariectomized animals as compared with non-ovariectomized animals. Conclusions: Estrogen deficiency associated with alcoholic diet, as well as estrogen deficiency (analyzed independently of diet type), decreased the immunostaining for OPG and increased the immunostaining for RANKL in the periodontium of rats.
publishDate 2018
dc.date.none.fl_str_mv 2018-12-11T17:37:44Z
2018-12-11T17:37:44Z
2018-11-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.archoralbio.2018.07.006
Archives of Oral Biology, v. 95, p. 7-14.
1879-1506
0003-9969
http://hdl.handle.net/11449/180032
10.1016/j.archoralbio.2018.07.006
2-s2.0-85049985658
2-s2.0-85049985658.pdf
5006685136114327
url http://dx.doi.org/10.1016/j.archoralbio.2018.07.006
http://hdl.handle.net/11449/180032
identifier_str_mv Archives of Oral Biology, v. 95, p. 7-14.
1879-1506
0003-9969
10.1016/j.archoralbio.2018.07.006
2-s2.0-85049985658
2-s2.0-85049985658.pdf
5006685136114327
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Archives of Oral Biology
0,752
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 7-14
application/pdf
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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