Selective photoinactivation of Histoplasma capsulatum by water-soluble derivatives chalcones
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.pdpdt.2017.03.001 http://hdl.handle.net/11449/165644 |
Resumo: | Histoplasmosis is a respiratory and systemic disease caused by the dimorphic fungus Histoplasma capsulatum. The clinical features may vary from asymptomatic infections to disseminated severe form depending of patient immunity. The treatment of histoplasmosis can be performed with itraconazole, fluconazole, and in the disseminated forms is used amphotericin B. However, the critical side effects of amphotericin B, the cases of itraconazole therapy failure and the appearance of fluconozole-resistant strains makes necessary the search of new strategies to treat this disease. Antimicrobial photodynamic therapy (aPDT) seems to be a potential candidate once have been show efficacy to inhibit others dimorphic fungi. Although the photosensitizer (PS) chalcone aggregates in biological medium, it has antifungal activity and show a high quantum yield of ROS formation. So, the aim of this study was to obtain the experimental parameters to achieve an acceptable selective chalcone water-soluble derivatives photoinactivation of H. capsulatum comparing with fibroblastic and keratinocytes cells which are the constituents of some potential host tissues. Yeast and cells were incubated with the same chalchones concentrations and short incubation time followed by irradiation with equal dose of light. The best conditions to kill H. capsulatum selectively were very low photosensitizers concentration (1.95 mu g mL(-1)) incubated by 15 min and irradiated with LED 450 nm with 24 J cm(-2). Key words: chalcone, Histoplasma capsulatum, aPDT, selectivity. (C) 2017 Elsevier B.V. All rights reserved. |
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Selective photoinactivation of Histoplasma capsulatum by water-soluble derivatives chalconesChalconeHistoplasma capsulatumaPDTSelectivityHistoplasmosis is a respiratory and systemic disease caused by the dimorphic fungus Histoplasma capsulatum. The clinical features may vary from asymptomatic infections to disseminated severe form depending of patient immunity. The treatment of histoplasmosis can be performed with itraconazole, fluconazole, and in the disseminated forms is used amphotericin B. However, the critical side effects of amphotericin B, the cases of itraconazole therapy failure and the appearance of fluconozole-resistant strains makes necessary the search of new strategies to treat this disease. Antimicrobial photodynamic therapy (aPDT) seems to be a potential candidate once have been show efficacy to inhibit others dimorphic fungi. Although the photosensitizer (PS) chalcone aggregates in biological medium, it has antifungal activity and show a high quantum yield of ROS formation. So, the aim of this study was to obtain the experimental parameters to achieve an acceptable selective chalcone water-soluble derivatives photoinactivation of H. capsulatum comparing with fibroblastic and keratinocytes cells which are the constituents of some potential host tissues. Yeast and cells were incubated with the same chalchones concentrations and short incubation time followed by irradiation with equal dose of light. The best conditions to kill H. capsulatum selectively were very low photosensitizers concentration (1.95 mu g mL(-1)) incubated by 15 min and irradiated with LED 450 nm with 24 J cm(-2). Key words: chalcone, Histoplasma capsulatum, aPDT, selectivity. (C) 2017 Elsevier B.V. All rights reserved.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Programa de Apoio ao Desenvolvimento Cientifico da Faculdade de Ciencias Farmaceuticas da UNESP (PADC)Univ Estadual Paulista, Nucleo Proteom, Biociencias Biotecnol Aplicada Farm, Araraquara, SP, BrazilUniv Estadual Paulista, Inst Biociencias Letras & Ciencias Exatas, Sao Jose Do Rio Preto, SP, BrazilUniv Estadual Paulista, Nucleo Proteom, Biociencias Biotecnol Aplicada Farm, Araraquara, SP, BrazilUniv Estadual Paulista, Inst Biociencias Letras & Ciencias Exatas, Sao Jose Do Rio Preto, SP, BrazilElsevier B.V.Universidade Estadual Paulista (Unesp)Melo, Wanessa C. M. A. [UNESP]Santos, Mariana Bastos dos [UNESP]Marques, Beatriz de Carvalho [UNESP]Regasini, Luis Octavio [UNESP]Soares Mendes Giannini, Maria Jose [UNESP]Fusco Almeida, Ana Marisa [UNESP]2018-11-28T14:21:45Z2018-11-28T14:21:45Z2017-06-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article232-235application/pdfhttp://dx.doi.org/10.1016/j.pdpdt.2017.03.001Photodiagnosis And Photodynamic Therapy. Amsterdam: Elsevier Science Bv, v. 18, p. 232-235, 2017.1572-1000http://hdl.handle.net/11449/16564410.1016/j.pdpdt.2017.03.001WOS:000404315000036WOS000404315000036.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPhotodiagnosis And Photodynamic Therapy0,647info:eu-repo/semantics/openAccess2024-06-21T15:19:08Zoai:repositorio.unesp.br:11449/165644Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T19:43:56.421604Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Selective photoinactivation of Histoplasma capsulatum by water-soluble derivatives chalcones |
title |
Selective photoinactivation of Histoplasma capsulatum by water-soluble derivatives chalcones |
spellingShingle |
Selective photoinactivation of Histoplasma capsulatum by water-soluble derivatives chalcones Melo, Wanessa C. M. A. [UNESP] Chalcone Histoplasma capsulatum aPDT Selectivity |
title_short |
Selective photoinactivation of Histoplasma capsulatum by water-soluble derivatives chalcones |
title_full |
Selective photoinactivation of Histoplasma capsulatum by water-soluble derivatives chalcones |
title_fullStr |
Selective photoinactivation of Histoplasma capsulatum by water-soluble derivatives chalcones |
title_full_unstemmed |
Selective photoinactivation of Histoplasma capsulatum by water-soluble derivatives chalcones |
title_sort |
Selective photoinactivation of Histoplasma capsulatum by water-soluble derivatives chalcones |
author |
Melo, Wanessa C. M. A. [UNESP] |
author_facet |
Melo, Wanessa C. M. A. [UNESP] Santos, Mariana Bastos dos [UNESP] Marques, Beatriz de Carvalho [UNESP] Regasini, Luis Octavio [UNESP] Soares Mendes Giannini, Maria Jose [UNESP] Fusco Almeida, Ana Marisa [UNESP] |
author_role |
author |
author2 |
Santos, Mariana Bastos dos [UNESP] Marques, Beatriz de Carvalho [UNESP] Regasini, Luis Octavio [UNESP] Soares Mendes Giannini, Maria Jose [UNESP] Fusco Almeida, Ana Marisa [UNESP] |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Melo, Wanessa C. M. A. [UNESP] Santos, Mariana Bastos dos [UNESP] Marques, Beatriz de Carvalho [UNESP] Regasini, Luis Octavio [UNESP] Soares Mendes Giannini, Maria Jose [UNESP] Fusco Almeida, Ana Marisa [UNESP] |
dc.subject.por.fl_str_mv |
Chalcone Histoplasma capsulatum aPDT Selectivity |
topic |
Chalcone Histoplasma capsulatum aPDT Selectivity |
description |
Histoplasmosis is a respiratory and systemic disease caused by the dimorphic fungus Histoplasma capsulatum. The clinical features may vary from asymptomatic infections to disseminated severe form depending of patient immunity. The treatment of histoplasmosis can be performed with itraconazole, fluconazole, and in the disseminated forms is used amphotericin B. However, the critical side effects of amphotericin B, the cases of itraconazole therapy failure and the appearance of fluconozole-resistant strains makes necessary the search of new strategies to treat this disease. Antimicrobial photodynamic therapy (aPDT) seems to be a potential candidate once have been show efficacy to inhibit others dimorphic fungi. Although the photosensitizer (PS) chalcone aggregates in biological medium, it has antifungal activity and show a high quantum yield of ROS formation. So, the aim of this study was to obtain the experimental parameters to achieve an acceptable selective chalcone water-soluble derivatives photoinactivation of H. capsulatum comparing with fibroblastic and keratinocytes cells which are the constituents of some potential host tissues. Yeast and cells were incubated with the same chalchones concentrations and short incubation time followed by irradiation with equal dose of light. The best conditions to kill H. capsulatum selectively were very low photosensitizers concentration (1.95 mu g mL(-1)) incubated by 15 min and irradiated with LED 450 nm with 24 J cm(-2). Key words: chalcone, Histoplasma capsulatum, aPDT, selectivity. (C) 2017 Elsevier B.V. All rights reserved. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-06-01 2018-11-28T14:21:45Z 2018-11-28T14:21:45Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.pdpdt.2017.03.001 Photodiagnosis And Photodynamic Therapy. Amsterdam: Elsevier Science Bv, v. 18, p. 232-235, 2017. 1572-1000 http://hdl.handle.net/11449/165644 10.1016/j.pdpdt.2017.03.001 WOS:000404315000036 WOS000404315000036.pdf |
url |
http://dx.doi.org/10.1016/j.pdpdt.2017.03.001 http://hdl.handle.net/11449/165644 |
identifier_str_mv |
Photodiagnosis And Photodynamic Therapy. Amsterdam: Elsevier Science Bv, v. 18, p. 232-235, 2017. 1572-1000 10.1016/j.pdpdt.2017.03.001 WOS:000404315000036 WOS000404315000036.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Photodiagnosis And Photodynamic Therapy 0,647 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
232-235 application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier B.V. |
publisher.none.fl_str_mv |
Elsevier B.V. |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129110160965632 |