Selective photoinactivation of Histoplasma capsulatum by water-soluble derivatives chalcones

Detalhes bibliográficos
Autor(a) principal: Melo, Wanessa C. M. A. [UNESP]
Data de Publicação: 2017
Outros Autores: Santos, Mariana Bastos dos [UNESP], Marques, Beatriz de Carvalho [UNESP], Regasini, Luis Octavio [UNESP], Soares Mendes Giannini, Maria Jose [UNESP], Fusco Almeida, Ana Marisa [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.pdpdt.2017.03.001
http://hdl.handle.net/11449/165644
Resumo: Histoplasmosis is a respiratory and systemic disease caused by the dimorphic fungus Histoplasma capsulatum. The clinical features may vary from asymptomatic infections to disseminated severe form depending of patient immunity. The treatment of histoplasmosis can be performed with itraconazole, fluconazole, and in the disseminated forms is used amphotericin B. However, the critical side effects of amphotericin B, the cases of itraconazole therapy failure and the appearance of fluconozole-resistant strains makes necessary the search of new strategies to treat this disease. Antimicrobial photodynamic therapy (aPDT) seems to be a potential candidate once have been show efficacy to inhibit others dimorphic fungi. Although the photosensitizer (PS) chalcone aggregates in biological medium, it has antifungal activity and show a high quantum yield of ROS formation. So, the aim of this study was to obtain the experimental parameters to achieve an acceptable selective chalcone water-soluble derivatives photoinactivation of H. capsulatum comparing with fibroblastic and keratinocytes cells which are the constituents of some potential host tissues. Yeast and cells were incubated with the same chalchones concentrations and short incubation time followed by irradiation with equal dose of light. The best conditions to kill H. capsulatum selectively were very low photosensitizers concentration (1.95 mu g mL(-1)) incubated by 15 min and irradiated with LED 450 nm with 24 J cm(-2). Key words: chalcone, Histoplasma capsulatum, aPDT, selectivity. (C) 2017 Elsevier B.V. All rights reserved.
id UNSP_fa722cdf54e0c147e04878e696a8a949
oai_identifier_str oai:repositorio.unesp.br:11449/165644
network_acronym_str UNSP
network_name_str Repositório Institucional da UNESP
repository_id_str 2946
spelling Selective photoinactivation of Histoplasma capsulatum by water-soluble derivatives chalconesChalconeHistoplasma capsulatumaPDTSelectivityHistoplasmosis is a respiratory and systemic disease caused by the dimorphic fungus Histoplasma capsulatum. The clinical features may vary from asymptomatic infections to disseminated severe form depending of patient immunity. The treatment of histoplasmosis can be performed with itraconazole, fluconazole, and in the disseminated forms is used amphotericin B. However, the critical side effects of amphotericin B, the cases of itraconazole therapy failure and the appearance of fluconozole-resistant strains makes necessary the search of new strategies to treat this disease. Antimicrobial photodynamic therapy (aPDT) seems to be a potential candidate once have been show efficacy to inhibit others dimorphic fungi. Although the photosensitizer (PS) chalcone aggregates in biological medium, it has antifungal activity and show a high quantum yield of ROS formation. So, the aim of this study was to obtain the experimental parameters to achieve an acceptable selective chalcone water-soluble derivatives photoinactivation of H. capsulatum comparing with fibroblastic and keratinocytes cells which are the constituents of some potential host tissues. Yeast and cells were incubated with the same chalchones concentrations and short incubation time followed by irradiation with equal dose of light. The best conditions to kill H. capsulatum selectively were very low photosensitizers concentration (1.95 mu g mL(-1)) incubated by 15 min and irradiated with LED 450 nm with 24 J cm(-2). Key words: chalcone, Histoplasma capsulatum, aPDT, selectivity. (C) 2017 Elsevier B.V. All rights reserved.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Programa de Apoio ao Desenvolvimento Cientifico da Faculdade de Ciencias Farmaceuticas da UNESP (PADC)Univ Estadual Paulista, Nucleo Proteom, Biociencias Biotecnol Aplicada Farm, Araraquara, SP, BrazilUniv Estadual Paulista, Inst Biociencias Letras & Ciencias Exatas, Sao Jose Do Rio Preto, SP, BrazilUniv Estadual Paulista, Nucleo Proteom, Biociencias Biotecnol Aplicada Farm, Araraquara, SP, BrazilUniv Estadual Paulista, Inst Biociencias Letras & Ciencias Exatas, Sao Jose Do Rio Preto, SP, BrazilElsevier B.V.Universidade Estadual Paulista (Unesp)Melo, Wanessa C. M. A. [UNESP]Santos, Mariana Bastos dos [UNESP]Marques, Beatriz de Carvalho [UNESP]Regasini, Luis Octavio [UNESP]Soares Mendes Giannini, Maria Jose [UNESP]Fusco Almeida, Ana Marisa [UNESP]2018-11-28T14:21:45Z2018-11-28T14:21:45Z2017-06-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article232-235application/pdfhttp://dx.doi.org/10.1016/j.pdpdt.2017.03.001Photodiagnosis And Photodynamic Therapy. Amsterdam: Elsevier Science Bv, v. 18, p. 232-235, 2017.1572-1000http://hdl.handle.net/11449/16564410.1016/j.pdpdt.2017.03.001WOS:000404315000036WOS000404315000036.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPhotodiagnosis And Photodynamic Therapy0,647info:eu-repo/semantics/openAccess2024-06-21T15:19:08Zoai:repositorio.unesp.br:11449/165644Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T19:43:56.421604Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Selective photoinactivation of Histoplasma capsulatum by water-soluble derivatives chalcones
title Selective photoinactivation of Histoplasma capsulatum by water-soluble derivatives chalcones
spellingShingle Selective photoinactivation of Histoplasma capsulatum by water-soluble derivatives chalcones
Melo, Wanessa C. M. A. [UNESP]
Chalcone
Histoplasma capsulatum
aPDT
Selectivity
title_short Selective photoinactivation of Histoplasma capsulatum by water-soluble derivatives chalcones
title_full Selective photoinactivation of Histoplasma capsulatum by water-soluble derivatives chalcones
title_fullStr Selective photoinactivation of Histoplasma capsulatum by water-soluble derivatives chalcones
title_full_unstemmed Selective photoinactivation of Histoplasma capsulatum by water-soluble derivatives chalcones
title_sort Selective photoinactivation of Histoplasma capsulatum by water-soluble derivatives chalcones
author Melo, Wanessa C. M. A. [UNESP]
author_facet Melo, Wanessa C. M. A. [UNESP]
Santos, Mariana Bastos dos [UNESP]
Marques, Beatriz de Carvalho [UNESP]
Regasini, Luis Octavio [UNESP]
Soares Mendes Giannini, Maria Jose [UNESP]
Fusco Almeida, Ana Marisa [UNESP]
author_role author
author2 Santos, Mariana Bastos dos [UNESP]
Marques, Beatriz de Carvalho [UNESP]
Regasini, Luis Octavio [UNESP]
Soares Mendes Giannini, Maria Jose [UNESP]
Fusco Almeida, Ana Marisa [UNESP]
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Melo, Wanessa C. M. A. [UNESP]
Santos, Mariana Bastos dos [UNESP]
Marques, Beatriz de Carvalho [UNESP]
Regasini, Luis Octavio [UNESP]
Soares Mendes Giannini, Maria Jose [UNESP]
Fusco Almeida, Ana Marisa [UNESP]
dc.subject.por.fl_str_mv Chalcone
Histoplasma capsulatum
aPDT
Selectivity
topic Chalcone
Histoplasma capsulatum
aPDT
Selectivity
description Histoplasmosis is a respiratory and systemic disease caused by the dimorphic fungus Histoplasma capsulatum. The clinical features may vary from asymptomatic infections to disseminated severe form depending of patient immunity. The treatment of histoplasmosis can be performed with itraconazole, fluconazole, and in the disseminated forms is used amphotericin B. However, the critical side effects of amphotericin B, the cases of itraconazole therapy failure and the appearance of fluconozole-resistant strains makes necessary the search of new strategies to treat this disease. Antimicrobial photodynamic therapy (aPDT) seems to be a potential candidate once have been show efficacy to inhibit others dimorphic fungi. Although the photosensitizer (PS) chalcone aggregates in biological medium, it has antifungal activity and show a high quantum yield of ROS formation. So, the aim of this study was to obtain the experimental parameters to achieve an acceptable selective chalcone water-soluble derivatives photoinactivation of H. capsulatum comparing with fibroblastic and keratinocytes cells which are the constituents of some potential host tissues. Yeast and cells were incubated with the same chalchones concentrations and short incubation time followed by irradiation with equal dose of light. The best conditions to kill H. capsulatum selectively were very low photosensitizers concentration (1.95 mu g mL(-1)) incubated by 15 min and irradiated with LED 450 nm with 24 J cm(-2). Key words: chalcone, Histoplasma capsulatum, aPDT, selectivity. (C) 2017 Elsevier B.V. All rights reserved.
publishDate 2017
dc.date.none.fl_str_mv 2017-06-01
2018-11-28T14:21:45Z
2018-11-28T14:21:45Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.pdpdt.2017.03.001
Photodiagnosis And Photodynamic Therapy. Amsterdam: Elsevier Science Bv, v. 18, p. 232-235, 2017.
1572-1000
http://hdl.handle.net/11449/165644
10.1016/j.pdpdt.2017.03.001
WOS:000404315000036
WOS000404315000036.pdf
url http://dx.doi.org/10.1016/j.pdpdt.2017.03.001
http://hdl.handle.net/11449/165644
identifier_str_mv Photodiagnosis And Photodynamic Therapy. Amsterdam: Elsevier Science Bv, v. 18, p. 232-235, 2017.
1572-1000
10.1016/j.pdpdt.2017.03.001
WOS:000404315000036
WOS000404315000036.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Photodiagnosis And Photodynamic Therapy
0,647
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 232-235
application/pdf
dc.publisher.none.fl_str_mv Elsevier B.V.
publisher.none.fl_str_mv Elsevier B.V.
dc.source.none.fl_str_mv Web of Science
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1808129110160965632