Synthesis and evaluation of the potential deleterious effects of ZnO nanomaterials (nanoneedles and nanoflowers) on blood components, including albumin, erythrocytes and human isolated primary neutrophils
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1007/s11051-016-3527-6 http://hdl.handle.net/11449/173364 |
Resumo: | The application of zinc oxide (ZnO) nanoparticles in biomaterials has increased significantly in the recent years. Here, we aimed to study the potential deleterious effects of ZnO on blood components, including human serum albumin (HSA), erythrocytes and human isolated primary neutrophils. To test the influence of the morphology of the nanomaterials, ZnO nanoneedles (ZnO-nn) and nanoflowers (ZnO-nf) were synthesized. The zeta potential and mean size of ZnO-nf and ZnO-nn suspensions in phosphate-buffered saline were −10.73 mV and 3.81 nm and −5.27 mV and 18.26 nm, respectively. The incubation of ZnO with HSA did not cause its denaturation as verified by the absence of significant alterations in the intrinsic and extrinsic fluorescence and in the circular dichroism spectrum of the protein. The capacity of HSA as a drug carrier was not affected as verified by employing site I and II fluorescent markers. Neither type of ZnO was able to provoke the activation of neutrophils, as verified by lucigenin- and luminol-dependent chemiluminescence and by the extracellular release of hydrogen peroxide. ZnO-nf, but not ZnO-nn, induced the haemolysis of erythrocytes. In conclusion, our results reinforce the concept that ZnO nanomaterials are relatively safe for usage in biomaterials. A potential exception is the capacity of ZnO-nf to promote the lysis of erythrocytes, a discovery that shows the importance of the morphology in the toxicity of nanoparticles. |
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Synthesis and evaluation of the potential deleterious effects of ZnO nanomaterials (nanoneedles and nanoflowers) on blood components, including albumin, erythrocytes and human isolated primary neutrophilsErythrocytesHealth effectsHuman serum albuminMetal oxide nanoparticlesNeutrophilsSonochemistryZinc oxide nanoparticlesThe application of zinc oxide (ZnO) nanoparticles in biomaterials has increased significantly in the recent years. Here, we aimed to study the potential deleterious effects of ZnO on blood components, including human serum albumin (HSA), erythrocytes and human isolated primary neutrophils. To test the influence of the morphology of the nanomaterials, ZnO nanoneedles (ZnO-nn) and nanoflowers (ZnO-nf) were synthesized. The zeta potential and mean size of ZnO-nf and ZnO-nn suspensions in phosphate-buffered saline were −10.73 mV and 3.81 nm and −5.27 mV and 18.26 nm, respectively. The incubation of ZnO with HSA did not cause its denaturation as verified by the absence of significant alterations in the intrinsic and extrinsic fluorescence and in the circular dichroism spectrum of the protein. The capacity of HSA as a drug carrier was not affected as verified by employing site I and II fluorescent markers. Neither type of ZnO was able to provoke the activation of neutrophils, as verified by lucigenin- and luminol-dependent chemiluminescence and by the extracellular release of hydrogen peroxide. ZnO-nf, but not ZnO-nn, induced the haemolysis of erythrocytes. In conclusion, our results reinforce the concept that ZnO nanomaterials are relatively safe for usage in biomaterials. A potential exception is the capacity of ZnO-nf to promote the lysis of erythrocytes, a discovery that shows the importance of the morphology in the toxicity of nanoparticles.Department of Chemistry Faculty of Sciences São Paulo State University (UNESP), P.O. Box 473Department of Clinical Analysis School of Pharmaceutical Sciences São Paulo State University (UNESP)Nanomedicine and Nanotoxicology Group Physics Institute of São Carlos (IFSC) University of São Paulo (USP)Department of Physics Faculty of Sciences São Paulo State University (UNESP)Department of Chemistry Faculty of Sciences São Paulo State University (UNESP), P.O. Box 473Department of Clinical Analysis School of Pharmaceutical Sciences São Paulo State University (UNESP)Department of Physics Faculty of Sciences São Paulo State University (UNESP)Universidade Estadual Paulista (Unesp)Universidade de São Paulo (USP)Pastrello, Bruna [UNESP]Paracatu, Luana Chiquetto [UNESP]de Carvalho Bertozo, Luiza [UNESP]Paino, Iêda Maria MartinezLisboa-Filho, Paulo Noronha [UNESP]Ximenes, Valdecir Farias [UNESP]2018-12-11T17:04:50Z2018-12-11T17:04:50Z2016-06-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://dx.doi.org/10.1007/s11051-016-3527-6Journal of Nanoparticle Research, v. 18, n. 7, 2016.1572-896X1388-0764http://hdl.handle.net/11449/17336410.1007/s11051-016-3527-62-s2.0-849826939292-s2.0-84982693929.pdf13538624145320050000-0002-7734-4069Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Nanoparticle Research0,528info:eu-repo/semantics/openAccess2024-06-21T15:18:46Zoai:repositorio.unesp.br:11449/173364Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T17:41:32.545063Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Synthesis and evaluation of the potential deleterious effects of ZnO nanomaterials (nanoneedles and nanoflowers) on blood components, including albumin, erythrocytes and human isolated primary neutrophils |
title |
Synthesis and evaluation of the potential deleterious effects of ZnO nanomaterials (nanoneedles and nanoflowers) on blood components, including albumin, erythrocytes and human isolated primary neutrophils |
spellingShingle |
Synthesis and evaluation of the potential deleterious effects of ZnO nanomaterials (nanoneedles and nanoflowers) on blood components, including albumin, erythrocytes and human isolated primary neutrophils Pastrello, Bruna [UNESP] Erythrocytes Health effects Human serum albumin Metal oxide nanoparticles Neutrophils Sonochemistry Zinc oxide nanoparticles |
title_short |
Synthesis and evaluation of the potential deleterious effects of ZnO nanomaterials (nanoneedles and nanoflowers) on blood components, including albumin, erythrocytes and human isolated primary neutrophils |
title_full |
Synthesis and evaluation of the potential deleterious effects of ZnO nanomaterials (nanoneedles and nanoflowers) on blood components, including albumin, erythrocytes and human isolated primary neutrophils |
title_fullStr |
Synthesis and evaluation of the potential deleterious effects of ZnO nanomaterials (nanoneedles and nanoflowers) on blood components, including albumin, erythrocytes and human isolated primary neutrophils |
title_full_unstemmed |
Synthesis and evaluation of the potential deleterious effects of ZnO nanomaterials (nanoneedles and nanoflowers) on blood components, including albumin, erythrocytes and human isolated primary neutrophils |
title_sort |
Synthesis and evaluation of the potential deleterious effects of ZnO nanomaterials (nanoneedles and nanoflowers) on blood components, including albumin, erythrocytes and human isolated primary neutrophils |
author |
Pastrello, Bruna [UNESP] |
author_facet |
Pastrello, Bruna [UNESP] Paracatu, Luana Chiquetto [UNESP] de Carvalho Bertozo, Luiza [UNESP] Paino, Iêda Maria Martinez Lisboa-Filho, Paulo Noronha [UNESP] Ximenes, Valdecir Farias [UNESP] |
author_role |
author |
author2 |
Paracatu, Luana Chiquetto [UNESP] de Carvalho Bertozo, Luiza [UNESP] Paino, Iêda Maria Martinez Lisboa-Filho, Paulo Noronha [UNESP] Ximenes, Valdecir Farias [UNESP] |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
Pastrello, Bruna [UNESP] Paracatu, Luana Chiquetto [UNESP] de Carvalho Bertozo, Luiza [UNESP] Paino, Iêda Maria Martinez Lisboa-Filho, Paulo Noronha [UNESP] Ximenes, Valdecir Farias [UNESP] |
dc.subject.por.fl_str_mv |
Erythrocytes Health effects Human serum albumin Metal oxide nanoparticles Neutrophils Sonochemistry Zinc oxide nanoparticles |
topic |
Erythrocytes Health effects Human serum albumin Metal oxide nanoparticles Neutrophils Sonochemistry Zinc oxide nanoparticles |
description |
The application of zinc oxide (ZnO) nanoparticles in biomaterials has increased significantly in the recent years. Here, we aimed to study the potential deleterious effects of ZnO on blood components, including human serum albumin (HSA), erythrocytes and human isolated primary neutrophils. To test the influence of the morphology of the nanomaterials, ZnO nanoneedles (ZnO-nn) and nanoflowers (ZnO-nf) were synthesized. The zeta potential and mean size of ZnO-nf and ZnO-nn suspensions in phosphate-buffered saline were −10.73 mV and 3.81 nm and −5.27 mV and 18.26 nm, respectively. The incubation of ZnO with HSA did not cause its denaturation as verified by the absence of significant alterations in the intrinsic and extrinsic fluorescence and in the circular dichroism spectrum of the protein. The capacity of HSA as a drug carrier was not affected as verified by employing site I and II fluorescent markers. Neither type of ZnO was able to provoke the activation of neutrophils, as verified by lucigenin- and luminol-dependent chemiluminescence and by the extracellular release of hydrogen peroxide. ZnO-nf, but not ZnO-nn, induced the haemolysis of erythrocytes. In conclusion, our results reinforce the concept that ZnO nanomaterials are relatively safe for usage in biomaterials. A potential exception is the capacity of ZnO-nf to promote the lysis of erythrocytes, a discovery that shows the importance of the morphology in the toxicity of nanoparticles. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-06-01 2018-12-11T17:04:50Z 2018-12-11T17:04:50Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/s11051-016-3527-6 Journal of Nanoparticle Research, v. 18, n. 7, 2016. 1572-896X 1388-0764 http://hdl.handle.net/11449/173364 10.1007/s11051-016-3527-6 2-s2.0-84982693929 2-s2.0-84982693929.pdf 1353862414532005 0000-0002-7734-4069 |
url |
http://dx.doi.org/10.1007/s11051-016-3527-6 http://hdl.handle.net/11449/173364 |
identifier_str_mv |
Journal of Nanoparticle Research, v. 18, n. 7, 2016. 1572-896X 1388-0764 10.1007/s11051-016-3527-6 2-s2.0-84982693929 2-s2.0-84982693929.pdf 1353862414532005 0000-0002-7734-4069 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Nanoparticle Research 0,528 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1808128845725827072 |