A comparative genomic analysis of Xanthomonas arboricola pv. juglandis strains reveal hallmarks of mobile genetic elements in the adaptation and accelerated evolution of virulence
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.ygeno.2021.06.003 http://hdl.handle.net/11449/207831 |
Resumo: | Xanthomonas arboricola pv. juglandis (Xaj) is the most significant aboveground walnut bacterial pathogen. Disease management uses copper-based pesticides which induce pathogen resistance. We examined the genetic repertoire associated with adaptation and virulence evolution in Xaj. Comparative genomics of 32 Xaj strains reveal the possible acquisition and propagation of virulence factors via insertion sequences (IS). Fine-scale annotation revealed a Tn3 transposon (TnXaj417) encoding copper resistance genes acquired by horizontal gene transfer and associated with adaptation and tolerance to metal-based pesticides commonly used to manage pathogens in orchard ecosystems. Phylogenomic analysis reveals IS involvement in acquisition and diversification of type III effector proteins ranging from two to eight in non-pathogenic strains, 16 to 20 in pathogenic strains, besides six other putative effectors with a reduced identity degree found mostly among pathogenic strains. Yersiniabactin, xopK, xopAI, and antibiotic resistance genes are also located near ISs or inside genomic islands and structures resembling composite transposons. |
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A comparative genomic analysis of Xanthomonas arboricola pv. juglandis strains reveal hallmarks of mobile genetic elements in the adaptation and accelerated evolution of virulenceCopper resistanceGenome evolutionLateral gene transferMobile genetic elementsReplicative transpositionT3SS effectorsXanthomonas arboricola pv. juglandis (Xaj) is the most significant aboveground walnut bacterial pathogen. Disease management uses copper-based pesticides which induce pathogen resistance. We examined the genetic repertoire associated with adaptation and virulence evolution in Xaj. Comparative genomics of 32 Xaj strains reveal the possible acquisition and propagation of virulence factors via insertion sequences (IS). Fine-scale annotation revealed a Tn3 transposon (TnXaj417) encoding copper resistance genes acquired by horizontal gene transfer and associated with adaptation and tolerance to metal-based pesticides commonly used to manage pathogens in orchard ecosystems. Phylogenomic analysis reveals IS involvement in acquisition and diversification of type III effector proteins ranging from two to eight in non-pathogenic strains, 16 to 20 in pathogenic strains, besides six other putative effectors with a reduced identity degree found mostly among pathogenic strains. Yersiniabactin, xopK, xopAI, and antibiotic resistance genes are also located near ISs or inside genomic islands and structures resembling composite transposons.Center of Research in Biological Science Federal University of Ouro PretoDepartment of Plant Sciences University of CaliforniaFaculty of Agricultural and Veterinary Sciences of Jaboticabal (FCAV) Universidade Estadual Paulista (UNESP) Department of TechnologyCell Cycle Laboratory Butantan InstituteDepartment of Biochemistry Chemistry Institute University of Sao PauloSchool of Computing Federal University of Mato Grosso do SulDepartment of Biological Science Institute of Exact and Biological Science Federal University of Ouro PretoFaculty of Agricultural and Veterinary Sciences of Jaboticabal (FCAV) Universidade Estadual Paulista (UNESP) Department of TechnologyFederal University of Ouro PretoUniversity of CaliforniaUniversidade Estadual Paulista (Unesp)Butantan InstituteUniversidade de São Paulo (USP)Federal University of Mato Grosso do SulAssis, Renata A.B.Varani, Alessandro M. [UNESP]Sagawa, Cintia H.D.Patané, José S.L.Setubal, João CarlosUceda-Campos, Guillermoda Silva, Aline MariaZaini, Paulo A.Almeida, Nalvo F.Moreira, Leandro MarcioDandekar, Abhaya M.2021-06-25T11:01:46Z2021-06-25T11:01:46Z2021-07-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article2513-2525http://dx.doi.org/10.1016/j.ygeno.2021.06.003Genomics, v. 113, n. 4, p. 2513-2525, 2021.1089-86460888-7543http://hdl.handle.net/11449/20783110.1016/j.ygeno.2021.06.0032-s2.0-85107282901Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengGenomicsinfo:eu-repo/semantics/openAccess2024-06-07T15:31:34Zoai:repositorio.unesp.br:11449/207831Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T15:30:32.113687Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
A comparative genomic analysis of Xanthomonas arboricola pv. juglandis strains reveal hallmarks of mobile genetic elements in the adaptation and accelerated evolution of virulence |
title |
A comparative genomic analysis of Xanthomonas arboricola pv. juglandis strains reveal hallmarks of mobile genetic elements in the adaptation and accelerated evolution of virulence |
spellingShingle |
A comparative genomic analysis of Xanthomonas arboricola pv. juglandis strains reveal hallmarks of mobile genetic elements in the adaptation and accelerated evolution of virulence Assis, Renata A.B. Copper resistance Genome evolution Lateral gene transfer Mobile genetic elements Replicative transposition T3SS effectors |
title_short |
A comparative genomic analysis of Xanthomonas arboricola pv. juglandis strains reveal hallmarks of mobile genetic elements in the adaptation and accelerated evolution of virulence |
title_full |
A comparative genomic analysis of Xanthomonas arboricola pv. juglandis strains reveal hallmarks of mobile genetic elements in the adaptation and accelerated evolution of virulence |
title_fullStr |
A comparative genomic analysis of Xanthomonas arboricola pv. juglandis strains reveal hallmarks of mobile genetic elements in the adaptation and accelerated evolution of virulence |
title_full_unstemmed |
A comparative genomic analysis of Xanthomonas arboricola pv. juglandis strains reveal hallmarks of mobile genetic elements in the adaptation and accelerated evolution of virulence |
title_sort |
A comparative genomic analysis of Xanthomonas arboricola pv. juglandis strains reveal hallmarks of mobile genetic elements in the adaptation and accelerated evolution of virulence |
author |
Assis, Renata A.B. |
author_facet |
Assis, Renata A.B. Varani, Alessandro M. [UNESP] Sagawa, Cintia H.D. Patané, José S.L. Setubal, João Carlos Uceda-Campos, Guillermo da Silva, Aline Maria Zaini, Paulo A. Almeida, Nalvo F. Moreira, Leandro Marcio Dandekar, Abhaya M. |
author_role |
author |
author2 |
Varani, Alessandro M. [UNESP] Sagawa, Cintia H.D. Patané, José S.L. Setubal, João Carlos Uceda-Campos, Guillermo da Silva, Aline Maria Zaini, Paulo A. Almeida, Nalvo F. Moreira, Leandro Marcio Dandekar, Abhaya M. |
author2_role |
author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Federal University of Ouro Preto University of California Universidade Estadual Paulista (Unesp) Butantan Institute Universidade de São Paulo (USP) Federal University of Mato Grosso do Sul |
dc.contributor.author.fl_str_mv |
Assis, Renata A.B. Varani, Alessandro M. [UNESP] Sagawa, Cintia H.D. Patané, José S.L. Setubal, João Carlos Uceda-Campos, Guillermo da Silva, Aline Maria Zaini, Paulo A. Almeida, Nalvo F. Moreira, Leandro Marcio Dandekar, Abhaya M. |
dc.subject.por.fl_str_mv |
Copper resistance Genome evolution Lateral gene transfer Mobile genetic elements Replicative transposition T3SS effectors |
topic |
Copper resistance Genome evolution Lateral gene transfer Mobile genetic elements Replicative transposition T3SS effectors |
description |
Xanthomonas arboricola pv. juglandis (Xaj) is the most significant aboveground walnut bacterial pathogen. Disease management uses copper-based pesticides which induce pathogen resistance. We examined the genetic repertoire associated with adaptation and virulence evolution in Xaj. Comparative genomics of 32 Xaj strains reveal the possible acquisition and propagation of virulence factors via insertion sequences (IS). Fine-scale annotation revealed a Tn3 transposon (TnXaj417) encoding copper resistance genes acquired by horizontal gene transfer and associated with adaptation and tolerance to metal-based pesticides commonly used to manage pathogens in orchard ecosystems. Phylogenomic analysis reveals IS involvement in acquisition and diversification of type III effector proteins ranging from two to eight in non-pathogenic strains, 16 to 20 in pathogenic strains, besides six other putative effectors with a reduced identity degree found mostly among pathogenic strains. Yersiniabactin, xopK, xopAI, and antibiotic resistance genes are also located near ISs or inside genomic islands and structures resembling composite transposons. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-06-25T11:01:46Z 2021-06-25T11:01:46Z 2021-07-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.ygeno.2021.06.003 Genomics, v. 113, n. 4, p. 2513-2525, 2021. 1089-8646 0888-7543 http://hdl.handle.net/11449/207831 10.1016/j.ygeno.2021.06.003 2-s2.0-85107282901 |
url |
http://dx.doi.org/10.1016/j.ygeno.2021.06.003 http://hdl.handle.net/11449/207831 |
identifier_str_mv |
Genomics, v. 113, n. 4, p. 2513-2525, 2021. 1089-8646 0888-7543 10.1016/j.ygeno.2021.06.003 2-s2.0-85107282901 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Genomics |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
2513-2525 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1808128524391809024 |