Augmented anandamide signalling in the substantia nigra pars reticulata mediates panicolytic-like effects in mice confronted by Crotalus durissus terrificus pit vipers
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1007/s00213-022-06127-3 http://hdl.handle.net/11449/241666 |
Resumo: | Rationale: The endocannabinoid modulation of fear and anxiety due to the on-demand synthesis and degradation is supported by a large body of research. Although it has been proposed that anandamide (AEA) in the substantia nigra pars reticulata (SNpr) seems to be important for the organisation of innate fear-related behaviours, a role for endogenous AEA has yet to be clarified. Methods: Mice were treated with the fatty acid amide hydrolase (FAAH) selective inhibitor URB597 at different concentrations (0.01, 0.1, 1 nmol/0.1 µL) in the SNpr and confronted by rattlesnakes (Crotalus durissus terrificus). The most effective dose of URB597 (1 nmol) was also preceded by microinjections of the CB1 receptor antagonist AM251 (0.1 nmol) into the SNpr, and mice were then confronted by the venomous snake. Results: URB597 (0.1 and 1 nmol) in the SNpr decreased the expression of defensive behaviours such as defensive attention, escape, and time spent inside the burrow of mice confronted by rattlesnakes. Moreover, pretreatment of SNpr with AM251 suppressed these antiaversive effects of URB597 in this midbrain structure. Conclusion: Overall, these data clearly indicate that the panicolytic consequences of endogenous AEA enhancement in the SNpr are mediated by CB1 receptor signalling. |
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Repositório Institucional da UNESP |
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Augmented anandamide signalling in the substantia nigra pars reticulata mediates panicolytic-like effects in mice confronted by Crotalus durissus terrificus pit vipersAnandamideCrotalus durissus terrificus venomous pit viperPanic attacksPrey vs. venomous snake paradigmSubstantia nigra pars reticulata (SNpr)URB597Rationale: The endocannabinoid modulation of fear and anxiety due to the on-demand synthesis and degradation is supported by a large body of research. Although it has been proposed that anandamide (AEA) in the substantia nigra pars reticulata (SNpr) seems to be important for the organisation of innate fear-related behaviours, a role for endogenous AEA has yet to be clarified. Methods: Mice were treated with the fatty acid amide hydrolase (FAAH) selective inhibitor URB597 at different concentrations (0.01, 0.1, 1 nmol/0.1 µL) in the SNpr and confronted by rattlesnakes (Crotalus durissus terrificus). The most effective dose of URB597 (1 nmol) was also preceded by microinjections of the CB1 receptor antagonist AM251 (0.1 nmol) into the SNpr, and mice were then confronted by the venomous snake. Results: URB597 (0.1 and 1 nmol) in the SNpr decreased the expression of defensive behaviours such as defensive attention, escape, and time spent inside the burrow of mice confronted by rattlesnakes. Moreover, pretreatment of SNpr with AM251 suppressed these antiaversive effects of URB597 in this midbrain structure. Conclusion: Overall, these data clearly indicate that the panicolytic consequences of endogenous AEA enhancement in the SNpr are mediated by CB1 receptor signalling.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)German-Israeli Foundation for Scientific Research and DevelopmentLaboratory of Neuroanatomy and Neuropsychobiology Department of Pharmacology Ribeirão Preto Medical School of the University of São Paulo (FMRP-USP), Av. Bandeirantes, 3900, São PauloDepartment of Biological Sciences School of Science Humanities and Languages São Paulo State University (UNESP), São PauloBehavioural Neurosciences Institute (INeC), São PauloOphidiarium LNN-FMRP-USP/INeC Ribeirão Preto Medical School of the University of São Paulo, São PauloNAP-USP-Neurobiology of Emotions Research Centre (NuPNE) Ribeirão Preto Medical School of the University of São Paulo, São PauloLaboratory of Neuronal Plasticity Department of Stress Neurobiology and Neurogenetics Max Planck Institute of PsychiatryCentral Nervous System Diseases Research Boehringer Ingelheim Pharmaceuticals Gesellschaft Mit Beschränkter Haftung & Compagnie KommanditgesellschaftDepartment of Biological Sciences School of Science Humanities and Languages São Paulo State University (UNESP), São PauloFAPESP: 2007/01174-1FAPESP: 2012/03798-0FAPESP: 2017/11855-8FAPESP: 2018/03898-1FAPESP: 2020/15050-7German-Israeli Foundation for Scientific Research and Development: I-1442-421.13/2017Universidade de São Paulo (USP)Universidade Estadual Paulista (UNESP)Behavioural Neurosciences Institute (INeC)Max Planck Institute of PsychiatryBoehringer Ingelheim Pharmaceuticals Gesellschaft Mit Beschränkter Haftung & Compagnie KommanditgesellschaftAlmada, Rafael C. [UNESP]Falconi-Sobrinho, Luiz Lucianoda Silva, Juliana A.Wotjak, Carsten T.Coimbra, Norberto C.2023-03-01T21:15:49Z2023-03-01T21:15:49Z2022-09-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article2753-2769http://dx.doi.org/10.1007/s00213-022-06127-3Psychopharmacology, v. 239, n. 9, p. 2753-2769, 2022.1432-20720033-3158http://hdl.handle.net/11449/24166610.1007/s00213-022-06127-32-s2.0-85131303521Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPsychopharmacologyinfo:eu-repo/semantics/openAccess2023-03-01T21:15:49Zoai:repositorio.unesp.br:11449/241666Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-03-01T21:15:49Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Augmented anandamide signalling in the substantia nigra pars reticulata mediates panicolytic-like effects in mice confronted by Crotalus durissus terrificus pit vipers |
title |
Augmented anandamide signalling in the substantia nigra pars reticulata mediates panicolytic-like effects in mice confronted by Crotalus durissus terrificus pit vipers |
spellingShingle |
Augmented anandamide signalling in the substantia nigra pars reticulata mediates panicolytic-like effects in mice confronted by Crotalus durissus terrificus pit vipers Almada, Rafael C. [UNESP] Anandamide Crotalus durissus terrificus venomous pit viper Panic attacks Prey vs. venomous snake paradigm Substantia nigra pars reticulata (SNpr) URB597 |
title_short |
Augmented anandamide signalling in the substantia nigra pars reticulata mediates panicolytic-like effects in mice confronted by Crotalus durissus terrificus pit vipers |
title_full |
Augmented anandamide signalling in the substantia nigra pars reticulata mediates panicolytic-like effects in mice confronted by Crotalus durissus terrificus pit vipers |
title_fullStr |
Augmented anandamide signalling in the substantia nigra pars reticulata mediates panicolytic-like effects in mice confronted by Crotalus durissus terrificus pit vipers |
title_full_unstemmed |
Augmented anandamide signalling in the substantia nigra pars reticulata mediates panicolytic-like effects in mice confronted by Crotalus durissus terrificus pit vipers |
title_sort |
Augmented anandamide signalling in the substantia nigra pars reticulata mediates panicolytic-like effects in mice confronted by Crotalus durissus terrificus pit vipers |
author |
Almada, Rafael C. [UNESP] |
author_facet |
Almada, Rafael C. [UNESP] Falconi-Sobrinho, Luiz Luciano da Silva, Juliana A. Wotjak, Carsten T. Coimbra, Norberto C. |
author_role |
author |
author2 |
Falconi-Sobrinho, Luiz Luciano da Silva, Juliana A. Wotjak, Carsten T. Coimbra, Norberto C. |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade de São Paulo (USP) Universidade Estadual Paulista (UNESP) Behavioural Neurosciences Institute (INeC) Max Planck Institute of Psychiatry Boehringer Ingelheim Pharmaceuticals Gesellschaft Mit Beschränkter Haftung & Compagnie Kommanditgesellschaft |
dc.contributor.author.fl_str_mv |
Almada, Rafael C. [UNESP] Falconi-Sobrinho, Luiz Luciano da Silva, Juliana A. Wotjak, Carsten T. Coimbra, Norberto C. |
dc.subject.por.fl_str_mv |
Anandamide Crotalus durissus terrificus venomous pit viper Panic attacks Prey vs. venomous snake paradigm Substantia nigra pars reticulata (SNpr) URB597 |
topic |
Anandamide Crotalus durissus terrificus venomous pit viper Panic attacks Prey vs. venomous snake paradigm Substantia nigra pars reticulata (SNpr) URB597 |
description |
Rationale: The endocannabinoid modulation of fear and anxiety due to the on-demand synthesis and degradation is supported by a large body of research. Although it has been proposed that anandamide (AEA) in the substantia nigra pars reticulata (SNpr) seems to be important for the organisation of innate fear-related behaviours, a role for endogenous AEA has yet to be clarified. Methods: Mice were treated with the fatty acid amide hydrolase (FAAH) selective inhibitor URB597 at different concentrations (0.01, 0.1, 1 nmol/0.1 µL) in the SNpr and confronted by rattlesnakes (Crotalus durissus terrificus). The most effective dose of URB597 (1 nmol) was also preceded by microinjections of the CB1 receptor antagonist AM251 (0.1 nmol) into the SNpr, and mice were then confronted by the venomous snake. Results: URB597 (0.1 and 1 nmol) in the SNpr decreased the expression of defensive behaviours such as defensive attention, escape, and time spent inside the burrow of mice confronted by rattlesnakes. Moreover, pretreatment of SNpr with AM251 suppressed these antiaversive effects of URB597 in this midbrain structure. Conclusion: Overall, these data clearly indicate that the panicolytic consequences of endogenous AEA enhancement in the SNpr are mediated by CB1 receptor signalling. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-09-01 2023-03-01T21:15:49Z 2023-03-01T21:15:49Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/s00213-022-06127-3 Psychopharmacology, v. 239, n. 9, p. 2753-2769, 2022. 1432-2072 0033-3158 http://hdl.handle.net/11449/241666 10.1007/s00213-022-06127-3 2-s2.0-85131303521 |
url |
http://dx.doi.org/10.1007/s00213-022-06127-3 http://hdl.handle.net/11449/241666 |
identifier_str_mv |
Psychopharmacology, v. 239, n. 9, p. 2753-2769, 2022. 1432-2072 0033-3158 10.1007/s00213-022-06127-3 2-s2.0-85131303521 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Psychopharmacology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
2753-2769 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1803650336621068288 |