Treatment with tacrolimus enhances alveolar bone formation and decreases osteoclast number in the maxillae: A histomorphometric and ultrastructural study in rats
Autor(a) principal: | |
---|---|
Data de Publicação: | 2008 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://hdl.handle.net/11449/225312 |
Resumo: | Recent studies have suggested that tacrolimus monotherapy is a beneficial therapeutic alternative for the normalization of cyclosporin-induced bone loss in animal models and humans. The mechanism accounting for this action is unclear at present. In the present study, we attempted to determine the effect of tacrolimus monotherapy on alveolar bone using histological, histomorphometrical and transmission electron microscopy (TEM). Groups of rats (n=10 each) were treated with either tacrolimus (1mg/kg/ day, s.c.) or drug vehicle for 60 days. Fragments containing maxillary molars were processed for light microscopy to investigate the alveolar bone volume, trabecular separation, number of osteoclasts and osteoblasts, and transmission electron microscopy to investigate their ultrastructural basic phenotype. Treatment with tacrolimus monotherapy during 60 days may induce increases in alveolar bone volume (BV/TV,%; P<0.05) and a non-significant decrease in trabecular separation (Tb.Sp,mm; P>0.05), represented by a decrease in osteoclast number (N.Oc/BS; P<0.05) and maintenance of osteoblast number (N.Ob/BS; P>0.05). Osteoblasts were often observed as a continuous layer of active cells on the bone surface. Osteoclasts appeared to be detached from the resorbed bone surface, which was often filled by active osteoblasts and collagen-rich matrix. Moreover, osteoclasts in the treated group were frequently observed as inactive cells (without ruffled border, clear zone and detached from the bone surface). Within the limits of the present study, we conclude that tacrolimus leads to an increase in alveolar bone formation, which probably exerts action on osteoclasts. Tacrolimus could, therefore, play a crucial role in the control of both early osteoclast differentiations from precursors, as well as in functional activation. |
id |
UNSP_fb21f49effa4a6bc8dea310df0e6c37d |
---|---|
oai_identifier_str |
oai:repositorio.unesp.br:11449/225312 |
network_acronym_str |
UNSP |
network_name_str |
Repositório Institucional da UNESP |
repository_id_str |
2946 |
spelling |
Treatment with tacrolimus enhances alveolar bone formation and decreases osteoclast number in the maxillae: A histomorphometric and ultrastructural study in ratsOsteoblastsOsteoclastsTacrolimusRecent studies have suggested that tacrolimus monotherapy is a beneficial therapeutic alternative for the normalization of cyclosporin-induced bone loss in animal models and humans. The mechanism accounting for this action is unclear at present. In the present study, we attempted to determine the effect of tacrolimus monotherapy on alveolar bone using histological, histomorphometrical and transmission electron microscopy (TEM). Groups of rats (n=10 each) were treated with either tacrolimus (1mg/kg/ day, s.c.) or drug vehicle for 60 days. Fragments containing maxillary molars were processed for light microscopy to investigate the alveolar bone volume, trabecular separation, number of osteoclasts and osteoblasts, and transmission electron microscopy to investigate their ultrastructural basic phenotype. Treatment with tacrolimus monotherapy during 60 days may induce increases in alveolar bone volume (BV/TV,%; P<0.05) and a non-significant decrease in trabecular separation (Tb.Sp,mm; P>0.05), represented by a decrease in osteoclast number (N.Oc/BS; P<0.05) and maintenance of osteoblast number (N.Ob/BS; P>0.05). Osteoblasts were often observed as a continuous layer of active cells on the bone surface. Osteoclasts appeared to be detached from the resorbed bone surface, which was often filled by active osteoblasts and collagen-rich matrix. Moreover, osteoclasts in the treated group were frequently observed as inactive cells (without ruffled border, clear zone and detached from the bone surface). Within the limits of the present study, we conclude that tacrolimus leads to an increase in alveolar bone formation, which probably exerts action on osteoclasts. Tacrolimus could, therefore, play a crucial role in the control of both early osteoclast differentiations from precursors, as well as in functional activation.Department of Periodontics Dental School of Araraquara - UNESP State University of São Paulo, Araraquara, São PauloDepartment of Morphology Dental School of Araraquara - UNESP State University of São Paulo, Araraquara, São PauloDepartment of Physiology and Pathology Dental School of Araraquara - UNESP State University of São Paulo, Araraquara, São PauloDepartment of Periodontics Dental School of Araraquara - UNESP State University of São Paulo, Araraquara, São PauloDepartment of Morphology Dental School of Araraquara - UNESP State University of São Paulo, Araraquara, São PauloDepartment of Physiology and Pathology Dental School of Araraquara - UNESP State University of São Paulo, Araraquara, São PauloUniversidade Estadual Paulista (UNESP)Andia, Denise Carleto [UNESP]Nassar, Carlos Augusto [UNESP]Nassar, Patrícia Oehlmeyer [UNESP]Guimarães, Morgana Rodrigues [UNESP]Cerri, Paulo Sérgio [UNESP]Spolidorio, Luis Carlos [UNESP]2022-04-28T20:44:40Z2022-04-28T20:44:40Z2008-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1177-1184Histology and Histopathology, v. 23, n. 10, p. 1177-1184, 2008.0213-3911http://hdl.handle.net/11449/2253122-s2.0-54449092924Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengHistology and Histopathologyinfo:eu-repo/semantics/openAccess2024-09-27T15:15:11Zoai:repositorio.unesp.br:11449/225312Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-27T15:15:11Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Treatment with tacrolimus enhances alveolar bone formation and decreases osteoclast number in the maxillae: A histomorphometric and ultrastructural study in rats |
title |
Treatment with tacrolimus enhances alveolar bone formation and decreases osteoclast number in the maxillae: A histomorphometric and ultrastructural study in rats |
spellingShingle |
Treatment with tacrolimus enhances alveolar bone formation and decreases osteoclast number in the maxillae: A histomorphometric and ultrastructural study in rats Andia, Denise Carleto [UNESP] Osteoblasts Osteoclasts Tacrolimus |
title_short |
Treatment with tacrolimus enhances alveolar bone formation and decreases osteoclast number in the maxillae: A histomorphometric and ultrastructural study in rats |
title_full |
Treatment with tacrolimus enhances alveolar bone formation and decreases osteoclast number in the maxillae: A histomorphometric and ultrastructural study in rats |
title_fullStr |
Treatment with tacrolimus enhances alveolar bone formation and decreases osteoclast number in the maxillae: A histomorphometric and ultrastructural study in rats |
title_full_unstemmed |
Treatment with tacrolimus enhances alveolar bone formation and decreases osteoclast number in the maxillae: A histomorphometric and ultrastructural study in rats |
title_sort |
Treatment with tacrolimus enhances alveolar bone formation and decreases osteoclast number in the maxillae: A histomorphometric and ultrastructural study in rats |
author |
Andia, Denise Carleto [UNESP] |
author_facet |
Andia, Denise Carleto [UNESP] Nassar, Carlos Augusto [UNESP] Nassar, Patrícia Oehlmeyer [UNESP] Guimarães, Morgana Rodrigues [UNESP] Cerri, Paulo Sérgio [UNESP] Spolidorio, Luis Carlos [UNESP] |
author_role |
author |
author2 |
Nassar, Carlos Augusto [UNESP] Nassar, Patrícia Oehlmeyer [UNESP] Guimarães, Morgana Rodrigues [UNESP] Cerri, Paulo Sérgio [UNESP] Spolidorio, Luis Carlos [UNESP] |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) |
dc.contributor.author.fl_str_mv |
Andia, Denise Carleto [UNESP] Nassar, Carlos Augusto [UNESP] Nassar, Patrícia Oehlmeyer [UNESP] Guimarães, Morgana Rodrigues [UNESP] Cerri, Paulo Sérgio [UNESP] Spolidorio, Luis Carlos [UNESP] |
dc.subject.por.fl_str_mv |
Osteoblasts Osteoclasts Tacrolimus |
topic |
Osteoblasts Osteoclasts Tacrolimus |
description |
Recent studies have suggested that tacrolimus monotherapy is a beneficial therapeutic alternative for the normalization of cyclosporin-induced bone loss in animal models and humans. The mechanism accounting for this action is unclear at present. In the present study, we attempted to determine the effect of tacrolimus monotherapy on alveolar bone using histological, histomorphometrical and transmission electron microscopy (TEM). Groups of rats (n=10 each) were treated with either tacrolimus (1mg/kg/ day, s.c.) or drug vehicle for 60 days. Fragments containing maxillary molars were processed for light microscopy to investigate the alveolar bone volume, trabecular separation, number of osteoclasts and osteoblasts, and transmission electron microscopy to investigate their ultrastructural basic phenotype. Treatment with tacrolimus monotherapy during 60 days may induce increases in alveolar bone volume (BV/TV,%; P<0.05) and a non-significant decrease in trabecular separation (Tb.Sp,mm; P>0.05), represented by a decrease in osteoclast number (N.Oc/BS; P<0.05) and maintenance of osteoblast number (N.Ob/BS; P>0.05). Osteoblasts were often observed as a continuous layer of active cells on the bone surface. Osteoclasts appeared to be detached from the resorbed bone surface, which was often filled by active osteoblasts and collagen-rich matrix. Moreover, osteoclasts in the treated group were frequently observed as inactive cells (without ruffled border, clear zone and detached from the bone surface). Within the limits of the present study, we conclude that tacrolimus leads to an increase in alveolar bone formation, which probably exerts action on osteoclasts. Tacrolimus could, therefore, play a crucial role in the control of both early osteoclast differentiations from precursors, as well as in functional activation. |
publishDate |
2008 |
dc.date.none.fl_str_mv |
2008-12-01 2022-04-28T20:44:40Z 2022-04-28T20:44:40Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Histology and Histopathology, v. 23, n. 10, p. 1177-1184, 2008. 0213-3911 http://hdl.handle.net/11449/225312 2-s2.0-54449092924 |
identifier_str_mv |
Histology and Histopathology, v. 23, n. 10, p. 1177-1184, 2008. 0213-3911 2-s2.0-54449092924 |
url |
http://hdl.handle.net/11449/225312 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Histology and Histopathology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1177-1184 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
repositoriounesp@unesp.br |
_version_ |
1813546518456565760 |