Treatment with tacrolimus enhances alveolar bone formation and decreases osteoclast number in the maxillae: A histomorphometric and ultrastructural study in rats

Detalhes bibliográficos
Autor(a) principal: Andia, Denise Carleto [UNESP]
Data de Publicação: 2008
Outros Autores: Nassar, Carlos Augusto [UNESP], Nassar, Patrícia Oehlmeyer [UNESP], Guimarães, Morgana Rodrigues [UNESP], Cerri, Paulo Sérgio [UNESP], Spolidorio, Luis Carlos [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://hdl.handle.net/11449/225312
Resumo: Recent studies have suggested that tacrolimus monotherapy is a beneficial therapeutic alternative for the normalization of cyclosporin-induced bone loss in animal models and humans. The mechanism accounting for this action is unclear at present. In the present study, we attempted to determine the effect of tacrolimus monotherapy on alveolar bone using histological, histomorphometrical and transmission electron microscopy (TEM). Groups of rats (n=10 each) were treated with either tacrolimus (1mg/kg/ day, s.c.) or drug vehicle for 60 days. Fragments containing maxillary molars were processed for light microscopy to investigate the alveolar bone volume, trabecular separation, number of osteoclasts and osteoblasts, and transmission electron microscopy to investigate their ultrastructural basic phenotype. Treatment with tacrolimus monotherapy during 60 days may induce increases in alveolar bone volume (BV/TV,%; P<0.05) and a non-significant decrease in trabecular separation (Tb.Sp,mm; P>0.05), represented by a decrease in osteoclast number (N.Oc/BS; P<0.05) and maintenance of osteoblast number (N.Ob/BS; P>0.05). Osteoblasts were often observed as a continuous layer of active cells on the bone surface. Osteoclasts appeared to be detached from the resorbed bone surface, which was often filled by active osteoblasts and collagen-rich matrix. Moreover, osteoclasts in the treated group were frequently observed as inactive cells (without ruffled border, clear zone and detached from the bone surface). Within the limits of the present study, we conclude that tacrolimus leads to an increase in alveolar bone formation, which probably exerts action on osteoclasts. Tacrolimus could, therefore, play a crucial role in the control of both early osteoclast differentiations from precursors, as well as in functional activation.
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spelling Treatment with tacrolimus enhances alveolar bone formation and decreases osteoclast number in the maxillae: A histomorphometric and ultrastructural study in ratsOsteoblastsOsteoclastsTacrolimusRecent studies have suggested that tacrolimus monotherapy is a beneficial therapeutic alternative for the normalization of cyclosporin-induced bone loss in animal models and humans. The mechanism accounting for this action is unclear at present. In the present study, we attempted to determine the effect of tacrolimus monotherapy on alveolar bone using histological, histomorphometrical and transmission electron microscopy (TEM). Groups of rats (n=10 each) were treated with either tacrolimus (1mg/kg/ day, s.c.) or drug vehicle for 60 days. Fragments containing maxillary molars were processed for light microscopy to investigate the alveolar bone volume, trabecular separation, number of osteoclasts and osteoblasts, and transmission electron microscopy to investigate their ultrastructural basic phenotype. Treatment with tacrolimus monotherapy during 60 days may induce increases in alveolar bone volume (BV/TV,%; P<0.05) and a non-significant decrease in trabecular separation (Tb.Sp,mm; P>0.05), represented by a decrease in osteoclast number (N.Oc/BS; P<0.05) and maintenance of osteoblast number (N.Ob/BS; P>0.05). Osteoblasts were often observed as a continuous layer of active cells on the bone surface. Osteoclasts appeared to be detached from the resorbed bone surface, which was often filled by active osteoblasts and collagen-rich matrix. Moreover, osteoclasts in the treated group were frequently observed as inactive cells (without ruffled border, clear zone and detached from the bone surface). Within the limits of the present study, we conclude that tacrolimus leads to an increase in alveolar bone formation, which probably exerts action on osteoclasts. Tacrolimus could, therefore, play a crucial role in the control of both early osteoclast differentiations from precursors, as well as in functional activation.Department of Periodontics Dental School of Araraquara - UNESP State University of São Paulo, Araraquara, São PauloDepartment of Morphology Dental School of Araraquara - UNESP State University of São Paulo, Araraquara, São PauloDepartment of Physiology and Pathology Dental School of Araraquara - UNESP State University of São Paulo, Araraquara, São PauloDepartment of Periodontics Dental School of Araraquara - UNESP State University of São Paulo, Araraquara, São PauloDepartment of Morphology Dental School of Araraquara - UNESP State University of São Paulo, Araraquara, São PauloDepartment of Physiology and Pathology Dental School of Araraquara - UNESP State University of São Paulo, Araraquara, São PauloUniversidade Estadual Paulista (UNESP)Andia, Denise Carleto [UNESP]Nassar, Carlos Augusto [UNESP]Nassar, Patrícia Oehlmeyer [UNESP]Guimarães, Morgana Rodrigues [UNESP]Cerri, Paulo Sérgio [UNESP]Spolidorio, Luis Carlos [UNESP]2022-04-28T20:44:40Z2022-04-28T20:44:40Z2008-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1177-1184Histology and Histopathology, v. 23, n. 10, p. 1177-1184, 2008.0213-3911http://hdl.handle.net/11449/2253122-s2.0-54449092924Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengHistology and Histopathologyinfo:eu-repo/semantics/openAccess2024-09-27T15:15:11Zoai:repositorio.unesp.br:11449/225312Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-27T15:15:11Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Treatment with tacrolimus enhances alveolar bone formation and decreases osteoclast number in the maxillae: A histomorphometric and ultrastructural study in rats
title Treatment with tacrolimus enhances alveolar bone formation and decreases osteoclast number in the maxillae: A histomorphometric and ultrastructural study in rats
spellingShingle Treatment with tacrolimus enhances alveolar bone formation and decreases osteoclast number in the maxillae: A histomorphometric and ultrastructural study in rats
Andia, Denise Carleto [UNESP]
Osteoblasts
Osteoclasts
Tacrolimus
title_short Treatment with tacrolimus enhances alveolar bone formation and decreases osteoclast number in the maxillae: A histomorphometric and ultrastructural study in rats
title_full Treatment with tacrolimus enhances alveolar bone formation and decreases osteoclast number in the maxillae: A histomorphometric and ultrastructural study in rats
title_fullStr Treatment with tacrolimus enhances alveolar bone formation and decreases osteoclast number in the maxillae: A histomorphometric and ultrastructural study in rats
title_full_unstemmed Treatment with tacrolimus enhances alveolar bone formation and decreases osteoclast number in the maxillae: A histomorphometric and ultrastructural study in rats
title_sort Treatment with tacrolimus enhances alveolar bone formation and decreases osteoclast number in the maxillae: A histomorphometric and ultrastructural study in rats
author Andia, Denise Carleto [UNESP]
author_facet Andia, Denise Carleto [UNESP]
Nassar, Carlos Augusto [UNESP]
Nassar, Patrícia Oehlmeyer [UNESP]
Guimarães, Morgana Rodrigues [UNESP]
Cerri, Paulo Sérgio [UNESP]
Spolidorio, Luis Carlos [UNESP]
author_role author
author2 Nassar, Carlos Augusto [UNESP]
Nassar, Patrícia Oehlmeyer [UNESP]
Guimarães, Morgana Rodrigues [UNESP]
Cerri, Paulo Sérgio [UNESP]
Spolidorio, Luis Carlos [UNESP]
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (UNESP)
dc.contributor.author.fl_str_mv Andia, Denise Carleto [UNESP]
Nassar, Carlos Augusto [UNESP]
Nassar, Patrícia Oehlmeyer [UNESP]
Guimarães, Morgana Rodrigues [UNESP]
Cerri, Paulo Sérgio [UNESP]
Spolidorio, Luis Carlos [UNESP]
dc.subject.por.fl_str_mv Osteoblasts
Osteoclasts
Tacrolimus
topic Osteoblasts
Osteoclasts
Tacrolimus
description Recent studies have suggested that tacrolimus monotherapy is a beneficial therapeutic alternative for the normalization of cyclosporin-induced bone loss in animal models and humans. The mechanism accounting for this action is unclear at present. In the present study, we attempted to determine the effect of tacrolimus monotherapy on alveolar bone using histological, histomorphometrical and transmission electron microscopy (TEM). Groups of rats (n=10 each) were treated with either tacrolimus (1mg/kg/ day, s.c.) or drug vehicle for 60 days. Fragments containing maxillary molars were processed for light microscopy to investigate the alveolar bone volume, trabecular separation, number of osteoclasts and osteoblasts, and transmission electron microscopy to investigate their ultrastructural basic phenotype. Treatment with tacrolimus monotherapy during 60 days may induce increases in alveolar bone volume (BV/TV,%; P<0.05) and a non-significant decrease in trabecular separation (Tb.Sp,mm; P>0.05), represented by a decrease in osteoclast number (N.Oc/BS; P<0.05) and maintenance of osteoblast number (N.Ob/BS; P>0.05). Osteoblasts were often observed as a continuous layer of active cells on the bone surface. Osteoclasts appeared to be detached from the resorbed bone surface, which was often filled by active osteoblasts and collagen-rich matrix. Moreover, osteoclasts in the treated group were frequently observed as inactive cells (without ruffled border, clear zone and detached from the bone surface). Within the limits of the present study, we conclude that tacrolimus leads to an increase in alveolar bone formation, which probably exerts action on osteoclasts. Tacrolimus could, therefore, play a crucial role in the control of both early osteoclast differentiations from precursors, as well as in functional activation.
publishDate 2008
dc.date.none.fl_str_mv 2008-12-01
2022-04-28T20:44:40Z
2022-04-28T20:44:40Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv Histology and Histopathology, v. 23, n. 10, p. 1177-1184, 2008.
0213-3911
http://hdl.handle.net/11449/225312
2-s2.0-54449092924
identifier_str_mv Histology and Histopathology, v. 23, n. 10, p. 1177-1184, 2008.
0213-3911
2-s2.0-54449092924
url http://hdl.handle.net/11449/225312
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Histology and Histopathology
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 1177-1184
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv repositoriounesp@unesp.br
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