At1 receptor blockade improves myocardial functional performance in obesity

Detalhes bibliográficos
Autor(a) principal: de Oliveira-Junior, Silvio Assis
Data de Publicação: 2020
Outros Autores: Muzili, Nayara de Araújo, Carvalho, Marianna Rabelo de, Ota, Gabriel Elias, de Morais, Camila Souza, Vieira, Larissa Fregapani da Costa, Ortiz, Mateus Oliveira, Campos, Dijon H. S. [UNESP], Cezar, Marcelo Diacardia Mariano [UNESP], Okoshi, Marina Politi [UNESP], Okoshi, Katashi [UNESP], Cicogna, Antonio C. [UNESP], Martinez, Paula Felippe
Tipo de documento: Artigo
Idioma: eng
por
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.36660/abc.20190131
http://hdl.handle.net/11449/201155
Resumo: Background: Obesity has been associated with chronic activation of the renin-angiotensin-aldosterone system and with significant changes in cardiac performance. Objective: To assess the impact of a blockade of angiotensin-II receptor type 1 (AT1 receptor) on morphology and on myocardial functional performance in rats with high-fat diet-induced obesity. Methods: Wistar rats (n=48) were submitted to control (2.9 kcal/g) or high-fat (3.6 kcal/g) diet for 20 weeks. After the 16th week they were divided into four groups: Control (CO), Obese (OB), Control Losartan (CL) and Obese Losartan (OL). CL and OL received losartan (30 mg/kg/day) in drinking water for four weeks. Subsequently, body composition, systolic blood pressure (SBP) and echocardiographic variables were analyzed. Papillary muscle function was assessed at baseline with 2.50 mM calcium concentration ([Ca2+] ) and after inotropic maneuvers: post-pause potentiation (PPP), [Ca2+]o elevation, and during beta-adrenergic stimulationo with isoproterenol. Analysis of the results was performed by the Two-Way ANOVA and by the appropriate comparison test. The level of significance was set at 5%. Results: Although SBP change had been not maintained at the end of the experiment, obesity was associated with cardiac hypertrophy and with increased left ventricle posterior wall shortening velocity. In the study of papillary muscles in basal condition, CL showed lower developed tension maximum negative variation velocity (-dT/dt) than CO. The 60s PPP promoted lower-dT/dt and maximum developed tension (DT) in OB and CL compared with CO, and higher relative DT variation and maximum positive variation velocity (+dT/dt) in OL compared with CL and OB. Under 1.5, 2.0, and 2.5mM [Ca2+]o, the OL group showed higher-dT/dt than CL. Conclusion: Losartan improves myocardial function in high-fat diet-induced obesity.
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spelling At1 receptor blockade improves myocardial functional performance in obesityBloqueio de Receptores AT1 Melhora o Desempenho Funcional Miocárdico na ObesidadeAngiotensin II Type 1 Receptor Blockers/ therapeutic useCardiovascular DiseasesDiet, High-fat/methodsLosartan/therapeutic useObesityRatsBackground: Obesity has been associated with chronic activation of the renin-angiotensin-aldosterone system and with significant changes in cardiac performance. Objective: To assess the impact of a blockade of angiotensin-II receptor type 1 (AT1 receptor) on morphology and on myocardial functional performance in rats with high-fat diet-induced obesity. Methods: Wistar rats (n=48) were submitted to control (2.9 kcal/g) or high-fat (3.6 kcal/g) diet for 20 weeks. After the 16th week they were divided into four groups: Control (CO), Obese (OB), Control Losartan (CL) and Obese Losartan (OL). CL and OL received losartan (30 mg/kg/day) in drinking water for four weeks. Subsequently, body composition, systolic blood pressure (SBP) and echocardiographic variables were analyzed. Papillary muscle function was assessed at baseline with 2.50 mM calcium concentration ([Ca2+] ) and after inotropic maneuvers: post-pause potentiation (PPP), [Ca2+]o elevation, and during beta-adrenergic stimulationo with isoproterenol. Analysis of the results was performed by the Two-Way ANOVA and by the appropriate comparison test. The level of significance was set at 5%. Results: Although SBP change had been not maintained at the end of the experiment, obesity was associated with cardiac hypertrophy and with increased left ventricle posterior wall shortening velocity. In the study of papillary muscles in basal condition, CL showed lower developed tension maximum negative variation velocity (-dT/dt) than CO. The 60s PPP promoted lower-dT/dt and maximum developed tension (DT) in OB and CL compared with CO, and higher relative DT variation and maximum positive variation velocity (+dT/dt) in OL compared with CL and OB. Under 1.5, 2.0, and 2.5mM [Ca2+]o, the OL group showed higher-dT/dt than CL. Conclusion: Losartan improves myocardial function in high-fat diet-induced obesity.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Universidade Federal de Mato Grosso do SulFaculdade de Medicina de Botucatu – UNESPFaculdade de Ciências Sociais e Agrárias de Itapeva (FAIT)Universidade Estadual de São Paulo (UNESP) Departamento de Medicina InternaUniversidade Estadual Paulista Júlio de Mesquita Filho Faculdade de Medicina, Campus de BotucatuFaculdade de Medicina de Botucatu – UNESPUniversidade Estadual de São Paulo (UNESP) Departamento de Medicina InternaUniversidade Estadual Paulista Júlio de Mesquita Filho Faculdade de Medicina, Campus de BotucatuCAPES: 001CNPq: 422776/2016-5Universidade Federal de Mato Grosso do Sul (UFMS)Universidade Estadual Paulista (Unesp)Faculdade de Ciências Sociais e Agrárias de Itapeva (FAIT)de Oliveira-Junior, Silvio AssisMuzili, Nayara de AraújoCarvalho, Marianna Rabelo deOta, Gabriel Eliasde Morais, Camila SouzaVieira, Larissa Fregapani da CostaOrtiz, Mateus OliveiraCampos, Dijon H. S. [UNESP]Cezar, Marcelo Diacardia Mariano [UNESP]Okoshi, Marina Politi [UNESP]Okoshi, Katashi [UNESP]Cicogna, Antonio C. [UNESP]Martinez, Paula Felippe2020-12-12T02:25:29Z2020-12-12T02:25:29Z2020-07-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article17-28application/pdfhttp://dx.doi.org/10.36660/abc.20190131Arquivos Brasileiros de Cardiologia, v. 115, n. 1, p. 17-28, 2020.1678-41700066-782Xhttp://hdl.handle.net/11449/20115510.36660/abc.20190131S0066-782X20200008000172-s2.0-85089409957S0066-782X2020000800017.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengporArquivos Brasileiros de Cardiologiainfo:eu-repo/semantics/openAccess2023-11-12T06:14:04Zoai:repositorio.unesp.br:11449/201155Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T17:30:18.341919Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv At1 receptor blockade improves myocardial functional performance in obesity
Bloqueio de Receptores AT1 Melhora o Desempenho Funcional Miocárdico na Obesidade
title At1 receptor blockade improves myocardial functional performance in obesity
spellingShingle At1 receptor blockade improves myocardial functional performance in obesity
de Oliveira-Junior, Silvio Assis
Angiotensin II Type 1 Receptor Blockers/ therapeutic use
Cardiovascular Diseases
Diet, High-fat/methods
Losartan/therapeutic use
Obesity
Rats
title_short At1 receptor blockade improves myocardial functional performance in obesity
title_full At1 receptor blockade improves myocardial functional performance in obesity
title_fullStr At1 receptor blockade improves myocardial functional performance in obesity
title_full_unstemmed At1 receptor blockade improves myocardial functional performance in obesity
title_sort At1 receptor blockade improves myocardial functional performance in obesity
author de Oliveira-Junior, Silvio Assis
author_facet de Oliveira-Junior, Silvio Assis
Muzili, Nayara de Araújo
Carvalho, Marianna Rabelo de
Ota, Gabriel Elias
de Morais, Camila Souza
Vieira, Larissa Fregapani da Costa
Ortiz, Mateus Oliveira
Campos, Dijon H. S. [UNESP]
Cezar, Marcelo Diacardia Mariano [UNESP]
Okoshi, Marina Politi [UNESP]
Okoshi, Katashi [UNESP]
Cicogna, Antonio C. [UNESP]
Martinez, Paula Felippe
author_role author
author2 Muzili, Nayara de Araújo
Carvalho, Marianna Rabelo de
Ota, Gabriel Elias
de Morais, Camila Souza
Vieira, Larissa Fregapani da Costa
Ortiz, Mateus Oliveira
Campos, Dijon H. S. [UNESP]
Cezar, Marcelo Diacardia Mariano [UNESP]
Okoshi, Marina Politi [UNESP]
Okoshi, Katashi [UNESP]
Cicogna, Antonio C. [UNESP]
Martinez, Paula Felippe
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Federal de Mato Grosso do Sul (UFMS)
Universidade Estadual Paulista (Unesp)
Faculdade de Ciências Sociais e Agrárias de Itapeva (FAIT)
dc.contributor.author.fl_str_mv de Oliveira-Junior, Silvio Assis
Muzili, Nayara de Araújo
Carvalho, Marianna Rabelo de
Ota, Gabriel Elias
de Morais, Camila Souza
Vieira, Larissa Fregapani da Costa
Ortiz, Mateus Oliveira
Campos, Dijon H. S. [UNESP]
Cezar, Marcelo Diacardia Mariano [UNESP]
Okoshi, Marina Politi [UNESP]
Okoshi, Katashi [UNESP]
Cicogna, Antonio C. [UNESP]
Martinez, Paula Felippe
dc.subject.por.fl_str_mv Angiotensin II Type 1 Receptor Blockers/ therapeutic use
Cardiovascular Diseases
Diet, High-fat/methods
Losartan/therapeutic use
Obesity
Rats
topic Angiotensin II Type 1 Receptor Blockers/ therapeutic use
Cardiovascular Diseases
Diet, High-fat/methods
Losartan/therapeutic use
Obesity
Rats
description Background: Obesity has been associated with chronic activation of the renin-angiotensin-aldosterone system and with significant changes in cardiac performance. Objective: To assess the impact of a blockade of angiotensin-II receptor type 1 (AT1 receptor) on morphology and on myocardial functional performance in rats with high-fat diet-induced obesity. Methods: Wistar rats (n=48) were submitted to control (2.9 kcal/g) or high-fat (3.6 kcal/g) diet for 20 weeks. After the 16th week they were divided into four groups: Control (CO), Obese (OB), Control Losartan (CL) and Obese Losartan (OL). CL and OL received losartan (30 mg/kg/day) in drinking water for four weeks. Subsequently, body composition, systolic blood pressure (SBP) and echocardiographic variables were analyzed. Papillary muscle function was assessed at baseline with 2.50 mM calcium concentration ([Ca2+] ) and after inotropic maneuvers: post-pause potentiation (PPP), [Ca2+]o elevation, and during beta-adrenergic stimulationo with isoproterenol. Analysis of the results was performed by the Two-Way ANOVA and by the appropriate comparison test. The level of significance was set at 5%. Results: Although SBP change had been not maintained at the end of the experiment, obesity was associated with cardiac hypertrophy and with increased left ventricle posterior wall shortening velocity. In the study of papillary muscles in basal condition, CL showed lower developed tension maximum negative variation velocity (-dT/dt) than CO. The 60s PPP promoted lower-dT/dt and maximum developed tension (DT) in OB and CL compared with CO, and higher relative DT variation and maximum positive variation velocity (+dT/dt) in OL compared with CL and OB. Under 1.5, 2.0, and 2.5mM [Ca2+]o, the OL group showed higher-dT/dt than CL. Conclusion: Losartan improves myocardial function in high-fat diet-induced obesity.
publishDate 2020
dc.date.none.fl_str_mv 2020-12-12T02:25:29Z
2020-12-12T02:25:29Z
2020-07-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.36660/abc.20190131
Arquivos Brasileiros de Cardiologia, v. 115, n. 1, p. 17-28, 2020.
1678-4170
0066-782X
http://hdl.handle.net/11449/201155
10.36660/abc.20190131
S0066-782X2020000800017
2-s2.0-85089409957
S0066-782X2020000800017.pdf
url http://dx.doi.org/10.36660/abc.20190131
http://hdl.handle.net/11449/201155
identifier_str_mv Arquivos Brasileiros de Cardiologia, v. 115, n. 1, p. 17-28, 2020.
1678-4170
0066-782X
10.36660/abc.20190131
S0066-782X2020000800017
2-s2.0-85089409957
S0066-782X2020000800017.pdf
dc.language.iso.fl_str_mv eng
por
language eng
por
dc.relation.none.fl_str_mv Arquivos Brasileiros de Cardiologia
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 17-28
application/pdf
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
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