NCED expression is related to increased ABA biosynthesis and stomatal closure under aluminum stress
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.envexpbot.2021.104404 http://hdl.handle.net/11449/208387 |
Resumo: | Aluminum (Al)-induced decrease in leaf hydration has been associated with low gas exchange, especially stomatal conductance (gs). However, the mechanisms explaining these responses are unclear. Citrus limonia was exposed to 0 and 1480 μM Al in nutrient solution for 90 days to test whether the low gs and leaf hydration in plants exposed to Al is associated with increased 9-cis-epoxycarotenoid dioxygenase (NCED) gene expression and abscisic acid (ABA) biosynthesis. Relative leaf water content (RWC), water potential (Ψw) and gas exchange in the leaves, as well as leaf and root ClNCED3, ClNCED1 and ClNCED5 expression and accumulation of ABA and its metabolites (phaseic acid, dihydrophaseic acid, (+)-7′-hydroxy-ABA and ABA-β-D-glucosyl ester) were measured. Aluminum up-regulated ClNCED3 and induced ABA accumulation in the roots before impairments in leaf water status (low Ψw, RWC and gs) could be observed. Leaf ABA concentration increased from 7 to 90 days and this could be partially explained by the up-regulation of ClNCED3, ClNCED1 and ClNCED5 in this organ. Stomatal closure occurred concomitantly with the increase of ABA concentration, and this result provides further evidence of the role of ABA modulation of plant hydration under Al stress. |
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NCED expression is related to increased ABA biosynthesis and stomatal closure under aluminum stressABAAluminumCitrusNCED genesPlant signallingWater relationsAluminum (Al)-induced decrease in leaf hydration has been associated with low gas exchange, especially stomatal conductance (gs). However, the mechanisms explaining these responses are unclear. Citrus limonia was exposed to 0 and 1480 μM Al in nutrient solution for 90 days to test whether the low gs and leaf hydration in plants exposed to Al is associated with increased 9-cis-epoxycarotenoid dioxygenase (NCED) gene expression and abscisic acid (ABA) biosynthesis. Relative leaf water content (RWC), water potential (Ψw) and gas exchange in the leaves, as well as leaf and root ClNCED3, ClNCED1 and ClNCED5 expression and accumulation of ABA and its metabolites (phaseic acid, dihydrophaseic acid, (+)-7′-hydroxy-ABA and ABA-β-D-glucosyl ester) were measured. Aluminum up-regulated ClNCED3 and induced ABA accumulation in the roots before impairments in leaf water status (low Ψw, RWC and gs) could be observed. Leaf ABA concentration increased from 7 to 90 days and this could be partially explained by the up-regulation of ClNCED3, ClNCED1 and ClNCED5 in this organ. Stomatal closure occurred concomitantly with the increase of ABA concentration, and this result provides further evidence of the role of ABA modulation of plant hydration under Al stress.Programa de Pós-Graduação em Biologia Vegetal Departamento de Biodiversidade Instituto de Biociências Universidade Estadual Paulista UNESP, Av. 24-A, 1515Departamento de Ciências Biológicas Escola Superior de Agricultura “Luiz de Queiróz” Universidade de São Paulo ESALQ-USP, Av. Pádua Dias, 11Cranfield Soil and Agrifood Institute Cranfield University, College RdDepartamento de Botânica Instituto de Biocências Universidade de São Paulo USP, Rua do Matão, 14Programa de Pós-Graduação em Genética e Biologia Molecular Centro de Biologia Molecular e Engenharia Genética Instituto de Biologia Universidade Estadual de Campinas Avenida Cândido Rondon Cidade UniversitáriaDepartamento de Biodiversidade Instituto de Biociências Universidade Estadual Paulista UNESP, Av. 24-A, 1515Programa de Pós-Graduação em Biologia Vegetal Departamento de Biodiversidade Instituto de Biociências Universidade Estadual Paulista UNESP, Av. 24-A, 1515Departamento de Biodiversidade Instituto de Biociências Universidade Estadual Paulista UNESP, Av. 24-A, 1515Universidade Estadual Paulista (Unesp)Universidade de São Paulo (USP)Cranfield UniversityUniversidade Estadual de Campinas (UNICAMP)Gavassi, Marina Alves [UNESP]Silva, Giselle Schwab [UNESP]da Silva, Carolina de Marchi SantiagoThompson, Andrew J.Macleod, KyleOliveira, Paulo Marcelo RaynerCavalheiro, Mariana FeitosaDomingues, Douglas Silva [UNESP]Habermann, Gustavo [UNESP]2021-06-25T11:11:21Z2021-06-25T11:11:21Z2021-05-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.envexpbot.2021.104404Environmental and Experimental Botany, v. 185.0098-8472http://hdl.handle.net/11449/20838710.1016/j.envexpbot.2021.1044042-s2.0-85100558673Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengEnvironmental and Experimental Botanyinfo:eu-repo/semantics/openAccess2021-10-23T19:02:09Zoai:repositorio.unesp.br:11449/208387Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T21:35:14.790747Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
NCED expression is related to increased ABA biosynthesis and stomatal closure under aluminum stress |
title |
NCED expression is related to increased ABA biosynthesis and stomatal closure under aluminum stress |
spellingShingle |
NCED expression is related to increased ABA biosynthesis and stomatal closure under aluminum stress Gavassi, Marina Alves [UNESP] ABA Aluminum Citrus NCED genes Plant signalling Water relations |
title_short |
NCED expression is related to increased ABA biosynthesis and stomatal closure under aluminum stress |
title_full |
NCED expression is related to increased ABA biosynthesis and stomatal closure under aluminum stress |
title_fullStr |
NCED expression is related to increased ABA biosynthesis and stomatal closure under aluminum stress |
title_full_unstemmed |
NCED expression is related to increased ABA biosynthesis and stomatal closure under aluminum stress |
title_sort |
NCED expression is related to increased ABA biosynthesis and stomatal closure under aluminum stress |
author |
Gavassi, Marina Alves [UNESP] |
author_facet |
Gavassi, Marina Alves [UNESP] Silva, Giselle Schwab [UNESP] da Silva, Carolina de Marchi Santiago Thompson, Andrew J. Macleod, Kyle Oliveira, Paulo Marcelo Rayner Cavalheiro, Mariana Feitosa Domingues, Douglas Silva [UNESP] Habermann, Gustavo [UNESP] |
author_role |
author |
author2 |
Silva, Giselle Schwab [UNESP] da Silva, Carolina de Marchi Santiago Thompson, Andrew J. Macleod, Kyle Oliveira, Paulo Marcelo Rayner Cavalheiro, Mariana Feitosa Domingues, Douglas Silva [UNESP] Habermann, Gustavo [UNESP] |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade de São Paulo (USP) Cranfield University Universidade Estadual de Campinas (UNICAMP) |
dc.contributor.author.fl_str_mv |
Gavassi, Marina Alves [UNESP] Silva, Giselle Schwab [UNESP] da Silva, Carolina de Marchi Santiago Thompson, Andrew J. Macleod, Kyle Oliveira, Paulo Marcelo Rayner Cavalheiro, Mariana Feitosa Domingues, Douglas Silva [UNESP] Habermann, Gustavo [UNESP] |
dc.subject.por.fl_str_mv |
ABA Aluminum Citrus NCED genes Plant signalling Water relations |
topic |
ABA Aluminum Citrus NCED genes Plant signalling Water relations |
description |
Aluminum (Al)-induced decrease in leaf hydration has been associated with low gas exchange, especially stomatal conductance (gs). However, the mechanisms explaining these responses are unclear. Citrus limonia was exposed to 0 and 1480 μM Al in nutrient solution for 90 days to test whether the low gs and leaf hydration in plants exposed to Al is associated with increased 9-cis-epoxycarotenoid dioxygenase (NCED) gene expression and abscisic acid (ABA) biosynthesis. Relative leaf water content (RWC), water potential (Ψw) and gas exchange in the leaves, as well as leaf and root ClNCED3, ClNCED1 and ClNCED5 expression and accumulation of ABA and its metabolites (phaseic acid, dihydrophaseic acid, (+)-7′-hydroxy-ABA and ABA-β-D-glucosyl ester) were measured. Aluminum up-regulated ClNCED3 and induced ABA accumulation in the roots before impairments in leaf water status (low Ψw, RWC and gs) could be observed. Leaf ABA concentration increased from 7 to 90 days and this could be partially explained by the up-regulation of ClNCED3, ClNCED1 and ClNCED5 in this organ. Stomatal closure occurred concomitantly with the increase of ABA concentration, and this result provides further evidence of the role of ABA modulation of plant hydration under Al stress. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-06-25T11:11:21Z 2021-06-25T11:11:21Z 2021-05-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.envexpbot.2021.104404 Environmental and Experimental Botany, v. 185. 0098-8472 http://hdl.handle.net/11449/208387 10.1016/j.envexpbot.2021.104404 2-s2.0-85100558673 |
url |
http://dx.doi.org/10.1016/j.envexpbot.2021.104404 http://hdl.handle.net/11449/208387 |
identifier_str_mv |
Environmental and Experimental Botany, v. 185. 0098-8472 10.1016/j.envexpbot.2021.104404 2-s2.0-85100558673 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Environmental and Experimental Botany |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1808129339295793152 |