Bone morphogenetic protein 15 supplementation enhances cumulus expansion, nuclear maturation and progesterone production of in vitro-matured bovine cumulus-oocyte complexes
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1111/rda.13914 http://hdl.handle.net/11449/210716 |
Resumo: | In vitro embryo production (IVP) efficiency is reduced when compared to in vivo. The basic knowledge of bovine in vitro oocyte maturation (IVM) mechanisms provides support to improve in vitro embryo production yields. The present study assessed the effects of bone morphogenetic protein 15 (BMP15), fibroblast growth factor 16 (FGF16) and their combined action on cumulus cells (CC) expansion, oocyte and CC DNA fragmentation, oocyte nuclear maturation, energetic metabolism and progesterone production in bovine IVM. Cumulus-oocyte complexes (COC) were matured in control or supplemented media containing BMP15 (100 ng/ml), FGF16 (10 ng/ml) or BMP15 combined with FGF16; and assessed at 0 and 22 hr of IVM. BMP15 alone or its association with FGF16 enhanced cumulus expansion. BMP15 decreased DNA fragmentation in both CC and oocytes, and improved oocyte nuclear maturation rate. In addition, BMP15 increased CC progesterone production, an effect not previously reported. The present study reinforces previous data pointing to a beneficial influence of BMP15 during IVM, while providing novel evidence that the underlying mechanisms involve increased progesterone production. |
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Bone morphogenetic protein 15 supplementation enhances cumulus expansion, nuclear maturation and progesterone production of in vitro-matured bovine cumulus-oocyte complexesFGF16glucose consumptionin vitro maturationIVM mediumlactate productionoocyte DNA fragmentationIn vitro embryo production (IVP) efficiency is reduced when compared to in vivo. The basic knowledge of bovine in vitro oocyte maturation (IVM) mechanisms provides support to improve in vitro embryo production yields. The present study assessed the effects of bone morphogenetic protein 15 (BMP15), fibroblast growth factor 16 (FGF16) and their combined action on cumulus cells (CC) expansion, oocyte and CC DNA fragmentation, oocyte nuclear maturation, energetic metabolism and progesterone production in bovine IVM. Cumulus-oocyte complexes (COC) were matured in control or supplemented media containing BMP15 (100 ng/ml), FGF16 (10 ng/ml) or BMP15 combined with FGF16; and assessed at 0 and 22 hr of IVM. BMP15 alone or its association with FGF16 enhanced cumulus expansion. BMP15 decreased DNA fragmentation in both CC and oocytes, and improved oocyte nuclear maturation rate. In addition, BMP15 increased CC progesterone production, an effect not previously reported. The present study reinforces previous data pointing to a beneficial influence of BMP15 during IVM, while providing novel evidence that the underlying mechanisms involve increased progesterone production.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Univ Sao Paulo, Sch Vet Med & Anim Sci, Dept Anim Reprod, Ave Prof Dr Orlando Marques de Paiva 87, BR-05508 Sao Paulo, SP, BrazilState Univ Sao Paulo, Inst Biociences, Dept Struct & Funct Biol, Botucatu, SP, BrazilIst Clin Zucchi, Biogenesi Reprod Med Ctr, Monza, ItalyState Univ Sao Paulo, Inst Biociences, Dept Struct & Funct Biol, Botucatu, SP, BrazilFAPESP: 2013/06063-4CAPES: 001Wiley-BlackwellUniversidade de São Paulo (USP)Universidade Estadual Paulista (Unesp)Ist Clin ZucchiDelgado, Juliana de CarvalhoHamilton, Thais Rose dos SantosMendes, Camilla MotaSiqueira, Adriano Felipe PerezGoissis, Marcelo DemarchiBuratini, Jose [UNESP]Assumpcao, Mayra Elena Ortiz D'Avila2021-06-26T03:35:36Z2021-06-26T03:35:36Z2021-03-08info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article754-763http://dx.doi.org/10.1111/rda.13914Reproduction In Domestic Animals. Hoboken: Wiley, v. 56, n. 5, p. 754-763, 2021.0936-6768http://hdl.handle.net/11449/21071610.1111/rda.13914WOS:000626275800001Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengReproduction In Domestic Animalsinfo:eu-repo/semantics/openAccess2021-10-23T22:13:54Zoai:repositorio.unesp.br:11449/210716Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-23T22:13:54Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Bone morphogenetic protein 15 supplementation enhances cumulus expansion, nuclear maturation and progesterone production of in vitro-matured bovine cumulus-oocyte complexes |
title |
Bone morphogenetic protein 15 supplementation enhances cumulus expansion, nuclear maturation and progesterone production of in vitro-matured bovine cumulus-oocyte complexes |
spellingShingle |
Bone morphogenetic protein 15 supplementation enhances cumulus expansion, nuclear maturation and progesterone production of in vitro-matured bovine cumulus-oocyte complexes Delgado, Juliana de Carvalho FGF16 glucose consumption in vitro maturation IVM medium lactate production oocyte DNA fragmentation |
title_short |
Bone morphogenetic protein 15 supplementation enhances cumulus expansion, nuclear maturation and progesterone production of in vitro-matured bovine cumulus-oocyte complexes |
title_full |
Bone morphogenetic protein 15 supplementation enhances cumulus expansion, nuclear maturation and progesterone production of in vitro-matured bovine cumulus-oocyte complexes |
title_fullStr |
Bone morphogenetic protein 15 supplementation enhances cumulus expansion, nuclear maturation and progesterone production of in vitro-matured bovine cumulus-oocyte complexes |
title_full_unstemmed |
Bone morphogenetic protein 15 supplementation enhances cumulus expansion, nuclear maturation and progesterone production of in vitro-matured bovine cumulus-oocyte complexes |
title_sort |
Bone morphogenetic protein 15 supplementation enhances cumulus expansion, nuclear maturation and progesterone production of in vitro-matured bovine cumulus-oocyte complexes |
author |
Delgado, Juliana de Carvalho |
author_facet |
Delgado, Juliana de Carvalho Hamilton, Thais Rose dos Santos Mendes, Camilla Mota Siqueira, Adriano Felipe Perez Goissis, Marcelo Demarchi Buratini, Jose [UNESP] Assumpcao, Mayra Elena Ortiz D'Avila |
author_role |
author |
author2 |
Hamilton, Thais Rose dos Santos Mendes, Camilla Mota Siqueira, Adriano Felipe Perez Goissis, Marcelo Demarchi Buratini, Jose [UNESP] Assumpcao, Mayra Elena Ortiz D'Avila |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade de São Paulo (USP) Universidade Estadual Paulista (Unesp) Ist Clin Zucchi |
dc.contributor.author.fl_str_mv |
Delgado, Juliana de Carvalho Hamilton, Thais Rose dos Santos Mendes, Camilla Mota Siqueira, Adriano Felipe Perez Goissis, Marcelo Demarchi Buratini, Jose [UNESP] Assumpcao, Mayra Elena Ortiz D'Avila |
dc.subject.por.fl_str_mv |
FGF16 glucose consumption in vitro maturation IVM medium lactate production oocyte DNA fragmentation |
topic |
FGF16 glucose consumption in vitro maturation IVM medium lactate production oocyte DNA fragmentation |
description |
In vitro embryo production (IVP) efficiency is reduced when compared to in vivo. The basic knowledge of bovine in vitro oocyte maturation (IVM) mechanisms provides support to improve in vitro embryo production yields. The present study assessed the effects of bone morphogenetic protein 15 (BMP15), fibroblast growth factor 16 (FGF16) and their combined action on cumulus cells (CC) expansion, oocyte and CC DNA fragmentation, oocyte nuclear maturation, energetic metabolism and progesterone production in bovine IVM. Cumulus-oocyte complexes (COC) were matured in control or supplemented media containing BMP15 (100 ng/ml), FGF16 (10 ng/ml) or BMP15 combined with FGF16; and assessed at 0 and 22 hr of IVM. BMP15 alone or its association with FGF16 enhanced cumulus expansion. BMP15 decreased DNA fragmentation in both CC and oocytes, and improved oocyte nuclear maturation rate. In addition, BMP15 increased CC progesterone production, an effect not previously reported. The present study reinforces previous data pointing to a beneficial influence of BMP15 during IVM, while providing novel evidence that the underlying mechanisms involve increased progesterone production. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-06-26T03:35:36Z 2021-06-26T03:35:36Z 2021-03-08 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1111/rda.13914 Reproduction In Domestic Animals. Hoboken: Wiley, v. 56, n. 5, p. 754-763, 2021. 0936-6768 http://hdl.handle.net/11449/210716 10.1111/rda.13914 WOS:000626275800001 |
url |
http://dx.doi.org/10.1111/rda.13914 http://hdl.handle.net/11449/210716 |
identifier_str_mv |
Reproduction In Domestic Animals. Hoboken: Wiley, v. 56, n. 5, p. 754-763, 2021. 0936-6768 10.1111/rda.13914 WOS:000626275800001 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Reproduction In Domestic Animals |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
754-763 |
dc.publisher.none.fl_str_mv |
Wiley-Blackwell |
publisher.none.fl_str_mv |
Wiley-Blackwell |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799964467716423680 |