Growth Curve, Morphological and Molecular Characterization of Two Strains of Trypanosoma cruzi (Kinetoplastida, Trypanosomatidae) isolated from Triatoma sherlocki (Hemiptera, Reduviidae, Triatominae)

Kunii, Gabriela Kinue Watase [UNESP]; Falcone, Rossana [UNESP]; Clementino, Leandro da Costa [UNESP]; Rosa, João Aristeu da[UNESP]; Nascimento, Juliana Damieli; Belintani, Tiago; Oliveira, Jader de; Ribeiro, Aline Rimoldi

Growth Curve, Morphological and Molecular Characterization of Two Strains of Trypanosoma cruzi (Kinetoplastida, Trypanosomatidae) isolated from Triatoma sherlocki (Hemiptera, Reduviidae, Triatominae)

Detalhes bibliográficos
Autor(a) principal:	Kunii, Gabriela Kinue Watase [UNESP]
Data de Publicação:	2022
Outros Autores:	Falcone, Rossana [UNESP], Clementino, Leandro da Costa [UNESP], Rosa, João Aristeu da[UNESP], Nascimento, Juliana Damieli, Belintani, Tiago, Oliveira, Jader de, Ribeiro, Aline Rimoldi
Tipo de documento:	Artigo
Idioma:	eng
Título da fonte:	Repositório Institucional da UNESP
Texto Completo:	http://dx.doi.org/10.1590/0037-8682-0521-2021 http://hdl.handle.net/11449/239872
Resumo:	Background: Trypanosoma cruzi presents great variability in morphology, virulence, pathogenicity, avoidance of the host immune system, and antigenic constitution, associated with different clinical manifestations of the disease. Methods: Two strains of T. cruzi were cultivated in liver infusion tryptose to determine growth kinetics, morphometry and molecular characterization using restriction fragment length polymorphism polymerase chain reaction. Results: The biological parameters showed sharp growth by the 7th day. Morphologically, both strains showed short and thin forms and were classified as Group I. Conclusion: Group TcI presents cardiac manifestations and T. sherlocki is adapting to the home environment, requiring attention to future problems.

Metadados do item

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network_acronym_str	UNSP
network_name_str	Repositório Institucional da UNESP
repository_id_str	2946
spelling	Growth Curve, Morphological and Molecular Characterization of Two Strains of Trypanosoma cruzi (Kinetoplastida, Trypanosomatidae) isolated from Triatoma sherlocki (Hemiptera, Reduviidae, Triatominae)Chagas diseaseEpidemiologyTrypanosoma cruziVector biologyBackground: Trypanosoma cruzi presents great variability in morphology, virulence, pathogenicity, avoidance of the host immune system, and antigenic constitution, associated with different clinical manifestations of the disease. Methods: Two strains of T. cruzi were cultivated in liver infusion tryptose to determine growth kinetics, morphometry and molecular characterization using restriction fragment length polymorphism polymerase chain reaction. Results: The biological parameters showed sharp growth by the 7th day. Morphologically, both strains showed short and thin forms and were classified as Group I. Conclusion: Group TcI presents cardiac manifestations and T. sherlocki is adapting to the home environment, requiring attention to future problems.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Universidade Estadual Paulista “Júlio de Mesquita Filho” Faculdade de Ciências Farmacêuticas, SPUniversidade Estadual de Campinas Departamento de Biologia Animal, SPUniversidade de São Paulo Departamento de Epidemiologia, SPUniversidade Estadual de Campinas Departamento de Genética Evolução Microbiologia e Imunologia, SPUniversidade Estadual Paulista “Júlio de Mesquita Filho” Faculdade de Ciências Farmacêuticas, SPFAPESP: 2018/25413-0CNPq: 307 398/2018-8Universidade Estadual Paulista (UNESP)Universidade Estadual de Campinas (UNICAMP)Universidade de São Paulo (USP)Kunii, Gabriela Kinue Watase [UNESP]Falcone, Rossana [UNESP]Clementino, Leandro da Costa [UNESP]Rosa, João Aristeu da[UNESP]Nascimento, Juliana DamieliBelintani, TiagoOliveira, Jader deRibeiro, Aline Rimoldi2023-03-01T19:51:09Z2023-03-01T19:51:09Z2022-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1590/0037-8682-0521-2021Revista da Sociedade Brasileira de Medicina Tropical, v. 55.0037-8682http://hdl.handle.net/11449/23987210.1590/0037-8682-0521-20212-s2.0-85128154709Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengRevista da Sociedade Brasileira de Medicina Tropicalinfo:eu-repo/semantics/openAccess2023-03-01T19:51:09Zoai:repositorio.unesp.br:11449/239872Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-03-01T19:51:09Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv	Growth Curve, Morphological and Molecular Characterization of Two Strains of Trypanosoma cruzi (Kinetoplastida, Trypanosomatidae) isolated from Triatoma sherlocki (Hemiptera, Reduviidae, Triatominae)
title	Growth Curve, Morphological and Molecular Characterization of Two Strains of Trypanosoma cruzi (Kinetoplastida, Trypanosomatidae) isolated from Triatoma sherlocki (Hemiptera, Reduviidae, Triatominae)
spellingShingle	Growth Curve, Morphological and Molecular Characterization of Two Strains of Trypanosoma cruzi (Kinetoplastida, Trypanosomatidae) isolated from Triatoma sherlocki (Hemiptera, Reduviidae, Triatominae) Kunii, Gabriela Kinue Watase [UNESP] Chagas disease Epidemiology Trypanosoma cruzi Vector biology
title_short	Growth Curve, Morphological and Molecular Characterization of Two Strains of Trypanosoma cruzi (Kinetoplastida, Trypanosomatidae) isolated from Triatoma sherlocki (Hemiptera, Reduviidae, Triatominae)
title_full	Growth Curve, Morphological and Molecular Characterization of Two Strains of Trypanosoma cruzi (Kinetoplastida, Trypanosomatidae) isolated from Triatoma sherlocki (Hemiptera, Reduviidae, Triatominae)
title_fullStr	Growth Curve, Morphological and Molecular Characterization of Two Strains of Trypanosoma cruzi (Kinetoplastida, Trypanosomatidae) isolated from Triatoma sherlocki (Hemiptera, Reduviidae, Triatominae)
title_full_unstemmed	Growth Curve, Morphological and Molecular Characterization of Two Strains of Trypanosoma cruzi (Kinetoplastida, Trypanosomatidae) isolated from Triatoma sherlocki (Hemiptera, Reduviidae, Triatominae)
title_sort	Growth Curve, Morphological and Molecular Characterization of Two Strains of Trypanosoma cruzi (Kinetoplastida, Trypanosomatidae) isolated from Triatoma sherlocki (Hemiptera, Reduviidae, Triatominae)
author	Kunii, Gabriela Kinue Watase [UNESP]
author_facet	Kunii, Gabriela Kinue Watase [UNESP] Falcone, Rossana [UNESP] Clementino, Leandro da Costa [UNESP] Rosa, João Aristeu da[UNESP] Nascimento, Juliana Damieli Belintani, Tiago Oliveira, Jader de Ribeiro, Aline Rimoldi
author_role	author
author2	Falcone, Rossana [UNESP] Clementino, Leandro da Costa [UNESP] Rosa, João Aristeu da[UNESP] Nascimento, Juliana Damieli Belintani, Tiago Oliveira, Jader de Ribeiro, Aline Rimoldi
author2_role	author author author author author author author
dc.contributor.none.fl_str_mv	Universidade Estadual Paulista (UNESP) Universidade Estadual de Campinas (UNICAMP) Universidade de São Paulo (USP)
dc.contributor.author.fl_str_mv	Kunii, Gabriela Kinue Watase [UNESP] Falcone, Rossana [UNESP] Clementino, Leandro da Costa [UNESP] Rosa, João Aristeu da[UNESP] Nascimento, Juliana Damieli Belintani, Tiago Oliveira, Jader de Ribeiro, Aline Rimoldi
dc.subject.por.fl_str_mv	Chagas disease Epidemiology Trypanosoma cruzi Vector biology
topic	Chagas disease Epidemiology Trypanosoma cruzi Vector biology
description	Background: Trypanosoma cruzi presents great variability in morphology, virulence, pathogenicity, avoidance of the host immune system, and antigenic constitution, associated with different clinical manifestations of the disease. Methods: Two strains of T. cruzi were cultivated in liver infusion tryptose to determine growth kinetics, morphometry and molecular characterization using restriction fragment length polymorphism polymerase chain reaction. Results: The biological parameters showed sharp growth by the 7th day. Morphologically, both strains showed short and thin forms and were classified as Group I. Conclusion: Group TcI presents cardiac manifestations and T. sherlocki is adapting to the home environment, requiring attention to future problems.
publishDate	2022
dc.date.none.fl_str_mv	2022-01-01 2023-03-01T19:51:09Z 2023-03-01T19:51:09Z
dc.type.status.fl_str_mv	info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv	info:eu-repo/semantics/article
format	article
status_str	publishedVersion
dc.identifier.uri.fl_str_mv	http://dx.doi.org/10.1590/0037-8682-0521-2021 Revista da Sociedade Brasileira de Medicina Tropical, v. 55. 0037-8682 http://hdl.handle.net/11449/239872 10.1590/0037-8682-0521-2021 2-s2.0-85128154709
url	http://dx.doi.org/10.1590/0037-8682-0521-2021 http://hdl.handle.net/11449/239872
identifier_str_mv	Revista da Sociedade Brasileira de Medicina Tropical, v. 55. 0037-8682 10.1590/0037-8682-0521-2021 2-s2.0-85128154709
dc.language.iso.fl_str_mv	eng
language	eng
dc.relation.none.fl_str_mv	Revista da Sociedade Brasileira de Medicina Tropical
dc.rights.driver.fl_str_mv	info:eu-repo/semantics/openAccess
eu_rights_str_mv	openAccess
dc.source.none.fl_str_mv	Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP
instname_str	Universidade Estadual Paulista (UNESP)
instacron_str	UNESP
institution	UNESP
reponame_str	Repositório Institucional da UNESP
collection	Repositório Institucional da UNESP
repository.name.fl_str_mv	Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_	1803046174302666752

Growth Curve, Morphological and Molecular Characterization of Two Strains of Trypanosoma cruzi (Kinetoplastida, Trypanosomatidae) isolated from Triatoma sherlocki (Hemiptera, Reduviidae, Triatominae)

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