Growth Curve, Morphological and Molecular Characterization of Two Strains of Trypanosoma cruzi (Kinetoplastida, Trypanosomatidae) isolated from Triatoma sherlocki (Hemiptera, Reduviidae, Triatominae)

Detalhes bibliográficos
Autor(a) principal: Kunii, Gabriela Kinue Watase [UNESP]
Data de Publicação: 2022
Outros Autores: Falcone, Rossana [UNESP], Clementino, Leandro da Costa [UNESP], Rosa, João Aristeu da[UNESP], Nascimento, Juliana Damieli, Belintani, Tiago, Oliveira, Jader de, Ribeiro, Aline Rimoldi
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1590/0037-8682-0521-2021
http://hdl.handle.net/11449/239872
Resumo: Background: Trypanosoma cruzi presents great variability in morphology, virulence, pathogenicity, avoidance of the host immune system, and antigenic constitution, associated with different clinical manifestations of the disease. Methods: Two strains of T. cruzi were cultivated in liver infusion tryptose to determine growth kinetics, morphometry and molecular characterization using restriction fragment length polymorphism polymerase chain reaction. Results: The biological parameters showed sharp growth by the 7th day. Morphologically, both strains showed short and thin forms and were classified as Group I. Conclusion: Group TcI presents cardiac manifestations and T. sherlocki is adapting to the home environment, requiring attention to future problems.
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spelling Growth Curve, Morphological and Molecular Characterization of Two Strains of Trypanosoma cruzi (Kinetoplastida, Trypanosomatidae) isolated from Triatoma sherlocki (Hemiptera, Reduviidae, Triatominae)Chagas diseaseEpidemiologyTrypanosoma cruziVector biologyBackground: Trypanosoma cruzi presents great variability in morphology, virulence, pathogenicity, avoidance of the host immune system, and antigenic constitution, associated with different clinical manifestations of the disease. Methods: Two strains of T. cruzi were cultivated in liver infusion tryptose to determine growth kinetics, morphometry and molecular characterization using restriction fragment length polymorphism polymerase chain reaction. Results: The biological parameters showed sharp growth by the 7th day. Morphologically, both strains showed short and thin forms and were classified as Group I. Conclusion: Group TcI presents cardiac manifestations and T. sherlocki is adapting to the home environment, requiring attention to future problems.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Universidade Estadual Paulista “Júlio de Mesquita Filho” Faculdade de Ciências Farmacêuticas, SPUniversidade Estadual de Campinas Departamento de Biologia Animal, SPUniversidade de São Paulo Departamento de Epidemiologia, SPUniversidade Estadual de Campinas Departamento de Genética Evolução Microbiologia e Imunologia, SPUniversidade Estadual Paulista “Júlio de Mesquita Filho” Faculdade de Ciências Farmacêuticas, SPFAPESP: 2018/25413-0CNPq: 307 398/2018-8Universidade Estadual Paulista (UNESP)Universidade Estadual de Campinas (UNICAMP)Universidade de São Paulo (USP)Kunii, Gabriela Kinue Watase [UNESP]Falcone, Rossana [UNESP]Clementino, Leandro da Costa [UNESP]Rosa, João Aristeu da[UNESP]Nascimento, Juliana DamieliBelintani, TiagoOliveira, Jader deRibeiro, Aline Rimoldi2023-03-01T19:51:09Z2023-03-01T19:51:09Z2022-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1590/0037-8682-0521-2021Revista da Sociedade Brasileira de Medicina Tropical, v. 55.0037-8682http://hdl.handle.net/11449/23987210.1590/0037-8682-0521-20212-s2.0-85128154709Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengRevista da Sociedade Brasileira de Medicina Tropicalinfo:eu-repo/semantics/openAccess2023-03-01T19:51:09Zoai:repositorio.unesp.br:11449/239872Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T16:03:29.566320Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Growth Curve, Morphological and Molecular Characterization of Two Strains of Trypanosoma cruzi (Kinetoplastida, Trypanosomatidae) isolated from Triatoma sherlocki (Hemiptera, Reduviidae, Triatominae)
title Growth Curve, Morphological and Molecular Characterization of Two Strains of Trypanosoma cruzi (Kinetoplastida, Trypanosomatidae) isolated from Triatoma sherlocki (Hemiptera, Reduviidae, Triatominae)
spellingShingle Growth Curve, Morphological and Molecular Characterization of Two Strains of Trypanosoma cruzi (Kinetoplastida, Trypanosomatidae) isolated from Triatoma sherlocki (Hemiptera, Reduviidae, Triatominae)
Kunii, Gabriela Kinue Watase [UNESP]
Chagas disease
Epidemiology
Trypanosoma cruzi
Vector biology
title_short Growth Curve, Morphological and Molecular Characterization of Two Strains of Trypanosoma cruzi (Kinetoplastida, Trypanosomatidae) isolated from Triatoma sherlocki (Hemiptera, Reduviidae, Triatominae)
title_full Growth Curve, Morphological and Molecular Characterization of Two Strains of Trypanosoma cruzi (Kinetoplastida, Trypanosomatidae) isolated from Triatoma sherlocki (Hemiptera, Reduviidae, Triatominae)
title_fullStr Growth Curve, Morphological and Molecular Characterization of Two Strains of Trypanosoma cruzi (Kinetoplastida, Trypanosomatidae) isolated from Triatoma sherlocki (Hemiptera, Reduviidae, Triatominae)
title_full_unstemmed Growth Curve, Morphological and Molecular Characterization of Two Strains of Trypanosoma cruzi (Kinetoplastida, Trypanosomatidae) isolated from Triatoma sherlocki (Hemiptera, Reduviidae, Triatominae)
title_sort Growth Curve, Morphological and Molecular Characterization of Two Strains of Trypanosoma cruzi (Kinetoplastida, Trypanosomatidae) isolated from Triatoma sherlocki (Hemiptera, Reduviidae, Triatominae)
author Kunii, Gabriela Kinue Watase [UNESP]
author_facet Kunii, Gabriela Kinue Watase [UNESP]
Falcone, Rossana [UNESP]
Clementino, Leandro da Costa [UNESP]
Rosa, João Aristeu da[UNESP]
Nascimento, Juliana Damieli
Belintani, Tiago
Oliveira, Jader de
Ribeiro, Aline Rimoldi
author_role author
author2 Falcone, Rossana [UNESP]
Clementino, Leandro da Costa [UNESP]
Rosa, João Aristeu da[UNESP]
Nascimento, Juliana Damieli
Belintani, Tiago
Oliveira, Jader de
Ribeiro, Aline Rimoldi
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (UNESP)
Universidade Estadual de Campinas (UNICAMP)
Universidade de São Paulo (USP)
dc.contributor.author.fl_str_mv Kunii, Gabriela Kinue Watase [UNESP]
Falcone, Rossana [UNESP]
Clementino, Leandro da Costa [UNESP]
Rosa, João Aristeu da[UNESP]
Nascimento, Juliana Damieli
Belintani, Tiago
Oliveira, Jader de
Ribeiro, Aline Rimoldi
dc.subject.por.fl_str_mv Chagas disease
Epidemiology
Trypanosoma cruzi
Vector biology
topic Chagas disease
Epidemiology
Trypanosoma cruzi
Vector biology
description Background: Trypanosoma cruzi presents great variability in morphology, virulence, pathogenicity, avoidance of the host immune system, and antigenic constitution, associated with different clinical manifestations of the disease. Methods: Two strains of T. cruzi were cultivated in liver infusion tryptose to determine growth kinetics, morphometry and molecular characterization using restriction fragment length polymorphism polymerase chain reaction. Results: The biological parameters showed sharp growth by the 7th day. Morphologically, both strains showed short and thin forms and were classified as Group I. Conclusion: Group TcI presents cardiac manifestations and T. sherlocki is adapting to the home environment, requiring attention to future problems.
publishDate 2022
dc.date.none.fl_str_mv 2022-01-01
2023-03-01T19:51:09Z
2023-03-01T19:51:09Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/0037-8682-0521-2021
Revista da Sociedade Brasileira de Medicina Tropical, v. 55.
0037-8682
http://hdl.handle.net/11449/239872
10.1590/0037-8682-0521-2021
2-s2.0-85128154709
url http://dx.doi.org/10.1590/0037-8682-0521-2021
http://hdl.handle.net/11449/239872
identifier_str_mv Revista da Sociedade Brasileira de Medicina Tropical, v. 55.
0037-8682
10.1590/0037-8682-0521-2021
2-s2.0-85128154709
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Revista da Sociedade Brasileira de Medicina Tropical
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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