Dysbiosis and Predicted Functions of the Dental Biofilm of Dairy Goats with Periodontitis
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1007/s00248-022-02062-0 http://hdl.handle.net/11449/241997 |
Resumo: | Periodontitis is a polymicrobial biofilm-induced inflammatory disease associated with a dysbiotic microbial community and severely affects the health and welfare of animals. However, little is known regarding the dental microbiota associated with this disease in goats. In this study, we used high-throughput sequencing, network analysis, and predicted functions to investigate the microbiota of clinically healthy goats and those with periodontitis and identify possible pathogens and proteins associated with the disease. Dental microbiomes of goats with periodontitis were richer, and network analyses showed that the number of negative interactions was higher in the networks of animals with periodontitis. Based on the interrelationships, Porphyromonas, Fusobacterium, and Prevotella were suggested to play an important role in the dental microbiota associated with goat periodontitis. Protein families linked to translation, cytoplasmatic translation, and rRNA processing were more abundant in the dental microbiota of goats with periodontitis. In conclusion, the dental biofilm microbiota associated with goat periodontitis seems to be dysbiotic and has significant antagonistic interactions, which discriminate healthy animals from diseased animals and highlight the importance of key bacteria. Thus, these novel findings contribute to the evolution of knowledge regarding the etiopathogenesis of goat periodontitis and possibly to the development of periodontitis control measures. |
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Dysbiosis and Predicted Functions of the Dental Biofilm of Dairy Goats with PeriodontitisBiofilmCo-occurrence network analysisGoatHigh-throughput sequencingMicrobiotaPeriodontitisPeriodontitis is a polymicrobial biofilm-induced inflammatory disease associated with a dysbiotic microbial community and severely affects the health and welfare of animals. However, little is known regarding the dental microbiota associated with this disease in goats. In this study, we used high-throughput sequencing, network analysis, and predicted functions to investigate the microbiota of clinically healthy goats and those with periodontitis and identify possible pathogens and proteins associated with the disease. Dental microbiomes of goats with periodontitis were richer, and network analyses showed that the number of negative interactions was higher in the networks of animals with periodontitis. Based on the interrelationships, Porphyromonas, Fusobacterium, and Prevotella were suggested to play an important role in the dental microbiota associated with goat periodontitis. Protein families linked to translation, cytoplasmatic translation, and rRNA processing were more abundant in the dental microbiota of goats with periodontitis. In conclusion, the dental biofilm microbiota associated with goat periodontitis seems to be dysbiotic and has significant antagonistic interactions, which discriminate healthy animals from diseased animals and highlight the importance of key bacteria. Thus, these novel findings contribute to the evolution of knowledge regarding the etiopathogenesis of goat periodontitis and possibly to the development of periodontitis control measures.Department of Veterinary Medicine School of Veterinary Medicine and Animal Science Universidade Federal de Goiás (UFG), GoiasDepartment of Production and Animal Health School of Veterinary Medicine São Paulo State University (Unesp), Araçatuba CampusPostgraduate Program in Veterinary Medicine São Paulo State University (Unesp) Jaboticabal CampusSchool of Medicine Dentistry and Nursing University of GlasgowDepartment of Production and Animal Health School of Veterinary Medicine São Paulo State University (Unesp), Araçatuba CampusPostgraduate Program in Veterinary Medicine São Paulo State University (Unesp) Jaboticabal CampusUniversidade Federal de Goiás (UFG)Universidade Estadual Paulista (UNESP)University of GlasgowBorsanelli, Ana CarolinaAthayde, Flávia R. F. [UNESP]Saraiva, Júlia R. [UNESP]Riggio, Marcello P.Dutra, Iveraldo S. [UNESP]2023-03-02T06:29:30Z2023-03-02T06:29:30Z2022-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1007/s00248-022-02062-0Microbial Ecology.1432-184X0095-3628http://hdl.handle.net/11449/24199710.1007/s00248-022-02062-02-s2.0-85133236122Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengMicrobial Ecologyinfo:eu-repo/semantics/openAccess2024-09-04T19:16:05Zoai:repositorio.unesp.br:11449/241997Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-04T19:16:05Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Dysbiosis and Predicted Functions of the Dental Biofilm of Dairy Goats with Periodontitis |
title |
Dysbiosis and Predicted Functions of the Dental Biofilm of Dairy Goats with Periodontitis |
spellingShingle |
Dysbiosis and Predicted Functions of the Dental Biofilm of Dairy Goats with Periodontitis Borsanelli, Ana Carolina Biofilm Co-occurrence network analysis Goat High-throughput sequencing Microbiota Periodontitis |
title_short |
Dysbiosis and Predicted Functions of the Dental Biofilm of Dairy Goats with Periodontitis |
title_full |
Dysbiosis and Predicted Functions of the Dental Biofilm of Dairy Goats with Periodontitis |
title_fullStr |
Dysbiosis and Predicted Functions of the Dental Biofilm of Dairy Goats with Periodontitis |
title_full_unstemmed |
Dysbiosis and Predicted Functions of the Dental Biofilm of Dairy Goats with Periodontitis |
title_sort |
Dysbiosis and Predicted Functions of the Dental Biofilm of Dairy Goats with Periodontitis |
author |
Borsanelli, Ana Carolina |
author_facet |
Borsanelli, Ana Carolina Athayde, Flávia R. F. [UNESP] Saraiva, Júlia R. [UNESP] Riggio, Marcello P. Dutra, Iveraldo S. [UNESP] |
author_role |
author |
author2 |
Athayde, Flávia R. F. [UNESP] Saraiva, Júlia R. [UNESP] Riggio, Marcello P. Dutra, Iveraldo S. [UNESP] |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade Federal de Goiás (UFG) Universidade Estadual Paulista (UNESP) University of Glasgow |
dc.contributor.author.fl_str_mv |
Borsanelli, Ana Carolina Athayde, Flávia R. F. [UNESP] Saraiva, Júlia R. [UNESP] Riggio, Marcello P. Dutra, Iveraldo S. [UNESP] |
dc.subject.por.fl_str_mv |
Biofilm Co-occurrence network analysis Goat High-throughput sequencing Microbiota Periodontitis |
topic |
Biofilm Co-occurrence network analysis Goat High-throughput sequencing Microbiota Periodontitis |
description |
Periodontitis is a polymicrobial biofilm-induced inflammatory disease associated with a dysbiotic microbial community and severely affects the health and welfare of animals. However, little is known regarding the dental microbiota associated with this disease in goats. In this study, we used high-throughput sequencing, network analysis, and predicted functions to investigate the microbiota of clinically healthy goats and those with periodontitis and identify possible pathogens and proteins associated with the disease. Dental microbiomes of goats with periodontitis were richer, and network analyses showed that the number of negative interactions was higher in the networks of animals with periodontitis. Based on the interrelationships, Porphyromonas, Fusobacterium, and Prevotella were suggested to play an important role in the dental microbiota associated with goat periodontitis. Protein families linked to translation, cytoplasmatic translation, and rRNA processing were more abundant in the dental microbiota of goats with periodontitis. In conclusion, the dental biofilm microbiota associated with goat periodontitis seems to be dysbiotic and has significant antagonistic interactions, which discriminate healthy animals from diseased animals and highlight the importance of key bacteria. Thus, these novel findings contribute to the evolution of knowledge regarding the etiopathogenesis of goat periodontitis and possibly to the development of periodontitis control measures. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-01-01 2023-03-02T06:29:30Z 2023-03-02T06:29:30Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/s00248-022-02062-0 Microbial Ecology. 1432-184X 0095-3628 http://hdl.handle.net/11449/241997 10.1007/s00248-022-02062-0 2-s2.0-85133236122 |
url |
http://dx.doi.org/10.1007/s00248-022-02062-0 http://hdl.handle.net/11449/241997 |
identifier_str_mv |
Microbial Ecology. 1432-184X 0095-3628 10.1007/s00248-022-02062-0 2-s2.0-85133236122 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Microbial Ecology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
repositoriounesp@unesp.br |
_version_ |
1810021411795763200 |