New insights into the anti-erosive property of a sugarcane-derived cystatin: different vehicle of application and potential mechanism of action
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/1678-7757-2021-0698 http://hdl.handle.net/11449/241477 |
Resumo: | OBJECTIVE: A new sugarcane-derived cystatin (CaneCPI-5) showed anti-erosive properties when included in solutions and strong binding force to enamel, but the performance of this protein when added to gel formulations and its effect on surface free energy (SFE) requires further studies. 1) to evaluate the protective effect of gels containing different concentrations of CaneCPI-5 against initial enamel erosion (Experiment 1); and 2) to analyze the SFE (γS) after treating the enamel surface with CaneCPI-5 solution (Experiment 2). METHODOLOGY: In Experiment 1, 75 bovine enamel specimens were divided into five groups according to the gel treatments: placebo (negative control); 0.27%mucin+0.5%casein (positive control); 0.1 mg/mL CaneCPI-5; 1.0 mg/mL CaneCPI-5; or 2.0 mg/mL CaneCPI-5. Specimens were treated with the gels for 1 min, the AP was formed (human saliva) for 2 h and the specimens were incubated in 0.65% citric acid (pH=3.4) for 1 min. The percentage of surface hardness change (%SHC) was estimated. In Experiment 2, measurements were performed by an automatic goniometer using three probing liquids: diiodomethane, water and ethylene glycol. Specimens (n=10/group) remained untreated (control) or were treated with solution containing 0.1 mg/mL CaneCPI-5, air-dried for 45 min, and 0.5 µL of each liquid was dispensed on the surface to measure contact angles. RESULTS: Gels containing 0.1 and 1.0 mg/mL CaneCPI-5 significantly reduced %SHC compared to the other treatments (p<0.05). Treated enamel showed significantly lower γS than control, without changes in the apolar component (γSLW), but the polar component (γSAB=Lewis acid-base) became more negative (p<0.01). Moreover, CaneCPI-5 treatment showed higher γS - (electron-donor) values compared to control (p<0.01). CONCLUSIONS: Gels containing 0.1 mg/mL or 1.0 mg/mL CaneCPI-5 protected enamel against initial dental erosion. CaneCPI-5 increased the number of electron donor sites on the enamel surface, which may affect AP formation and could be a potential mechanism of action to protect from erosion. |
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New insights into the anti-erosive property of a sugarcane-derived cystatin: different vehicle of application and potential mechanism of actionOBJECTIVE: A new sugarcane-derived cystatin (CaneCPI-5) showed anti-erosive properties when included in solutions and strong binding force to enamel, but the performance of this protein when added to gel formulations and its effect on surface free energy (SFE) requires further studies. 1) to evaluate the protective effect of gels containing different concentrations of CaneCPI-5 against initial enamel erosion (Experiment 1); and 2) to analyze the SFE (γS) after treating the enamel surface with CaneCPI-5 solution (Experiment 2). METHODOLOGY: In Experiment 1, 75 bovine enamel specimens were divided into five groups according to the gel treatments: placebo (negative control); 0.27%mucin+0.5%casein (positive control); 0.1 mg/mL CaneCPI-5; 1.0 mg/mL CaneCPI-5; or 2.0 mg/mL CaneCPI-5. Specimens were treated with the gels for 1 min, the AP was formed (human saliva) for 2 h and the specimens were incubated in 0.65% citric acid (pH=3.4) for 1 min. The percentage of surface hardness change (%SHC) was estimated. In Experiment 2, measurements were performed by an automatic goniometer using three probing liquids: diiodomethane, water and ethylene glycol. Specimens (n=10/group) remained untreated (control) or were treated with solution containing 0.1 mg/mL CaneCPI-5, air-dried for 45 min, and 0.5 µL of each liquid was dispensed on the surface to measure contact angles. RESULTS: Gels containing 0.1 and 1.0 mg/mL CaneCPI-5 significantly reduced %SHC compared to the other treatments (p<0.05). Treated enamel showed significantly lower γS than control, without changes in the apolar component (γSLW), but the polar component (γSAB=Lewis acid-base) became more negative (p<0.01). Moreover, CaneCPI-5 treatment showed higher γS - (electron-donor) values compared to control (p<0.01). CONCLUSIONS: Gels containing 0.1 mg/mL or 1.0 mg/mL CaneCPI-5 protected enamel against initial dental erosion. CaneCPI-5 increased the number of electron donor sites on the enamel surface, which may affect AP formation and could be a potential mechanism of action to protect from erosion.Universidade de São Paulo Departamento de Ciências Biológicas, Faculdade de Odontologia de BauruUniversidade Federal de São Carlos Departamento de Genética e EvoluçãoUniversidade Estadual Paulista Faculdade de Odontologia de Araçatuba Departamento de Odontologia Preventiva e RestauradoraUniversidade Estadual Paulista Faculdade de Odontologia de Araçatuba Departamento de Odontologia Preventiva e RestauradoraUniversidade de São Paulo (USP)Universidade Federal de São Carlos (UFSCar)Universidade Estadual Paulista (UNESP)Gironda, Carlos CondarcoPelá, Vinícius TaioquiHenrique-Silva, FlávioDelbem, Alberto Carlos Botazzo [UNESP]Pessan, Juliano Pelim [UNESP]Buzalaf, Marília Afonso Rabelo2023-03-01T21:05:03Z2023-03-01T21:05:03Z2022-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlee20210698http://dx.doi.org/10.1590/1678-7757-2021-0698Journal of applied oral science : revista FOB, v. 30, p. e20210698-.1678-7765http://hdl.handle.net/11449/24147710.1590/1678-7757-2021-06982-s2.0-85135501628Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of applied oral science : revista FOBinfo:eu-repo/semantics/openAccess2023-03-01T21:05:03Zoai:repositorio.unesp.br:11449/241477Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-03-01T21:05:03Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
New insights into the anti-erosive property of a sugarcane-derived cystatin: different vehicle of application and potential mechanism of action |
title |
New insights into the anti-erosive property of a sugarcane-derived cystatin: different vehicle of application and potential mechanism of action |
spellingShingle |
New insights into the anti-erosive property of a sugarcane-derived cystatin: different vehicle of application and potential mechanism of action Gironda, Carlos Condarco |
title_short |
New insights into the anti-erosive property of a sugarcane-derived cystatin: different vehicle of application and potential mechanism of action |
title_full |
New insights into the anti-erosive property of a sugarcane-derived cystatin: different vehicle of application and potential mechanism of action |
title_fullStr |
New insights into the anti-erosive property of a sugarcane-derived cystatin: different vehicle of application and potential mechanism of action |
title_full_unstemmed |
New insights into the anti-erosive property of a sugarcane-derived cystatin: different vehicle of application and potential mechanism of action |
title_sort |
New insights into the anti-erosive property of a sugarcane-derived cystatin: different vehicle of application and potential mechanism of action |
author |
Gironda, Carlos Condarco |
author_facet |
Gironda, Carlos Condarco Pelá, Vinícius Taioqui Henrique-Silva, Flávio Delbem, Alberto Carlos Botazzo [UNESP] Pessan, Juliano Pelim [UNESP] Buzalaf, Marília Afonso Rabelo |
author_role |
author |
author2 |
Pelá, Vinícius Taioqui Henrique-Silva, Flávio Delbem, Alberto Carlos Botazzo [UNESP] Pessan, Juliano Pelim [UNESP] Buzalaf, Marília Afonso Rabelo |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade de São Paulo (USP) Universidade Federal de São Carlos (UFSCar) Universidade Estadual Paulista (UNESP) |
dc.contributor.author.fl_str_mv |
Gironda, Carlos Condarco Pelá, Vinícius Taioqui Henrique-Silva, Flávio Delbem, Alberto Carlos Botazzo [UNESP] Pessan, Juliano Pelim [UNESP] Buzalaf, Marília Afonso Rabelo |
description |
OBJECTIVE: A new sugarcane-derived cystatin (CaneCPI-5) showed anti-erosive properties when included in solutions and strong binding force to enamel, but the performance of this protein when added to gel formulations and its effect on surface free energy (SFE) requires further studies. 1) to evaluate the protective effect of gels containing different concentrations of CaneCPI-5 against initial enamel erosion (Experiment 1); and 2) to analyze the SFE (γS) after treating the enamel surface with CaneCPI-5 solution (Experiment 2). METHODOLOGY: In Experiment 1, 75 bovine enamel specimens were divided into five groups according to the gel treatments: placebo (negative control); 0.27%mucin+0.5%casein (positive control); 0.1 mg/mL CaneCPI-5; 1.0 mg/mL CaneCPI-5; or 2.0 mg/mL CaneCPI-5. Specimens were treated with the gels for 1 min, the AP was formed (human saliva) for 2 h and the specimens were incubated in 0.65% citric acid (pH=3.4) for 1 min. The percentage of surface hardness change (%SHC) was estimated. In Experiment 2, measurements were performed by an automatic goniometer using three probing liquids: diiodomethane, water and ethylene glycol. Specimens (n=10/group) remained untreated (control) or were treated with solution containing 0.1 mg/mL CaneCPI-5, air-dried for 45 min, and 0.5 µL of each liquid was dispensed on the surface to measure contact angles. RESULTS: Gels containing 0.1 and 1.0 mg/mL CaneCPI-5 significantly reduced %SHC compared to the other treatments (p<0.05). Treated enamel showed significantly lower γS than control, without changes in the apolar component (γSLW), but the polar component (γSAB=Lewis acid-base) became more negative (p<0.01). Moreover, CaneCPI-5 treatment showed higher γS - (electron-donor) values compared to control (p<0.01). CONCLUSIONS: Gels containing 0.1 mg/mL or 1.0 mg/mL CaneCPI-5 protected enamel against initial dental erosion. CaneCPI-5 increased the number of electron donor sites on the enamel surface, which may affect AP formation and could be a potential mechanism of action to protect from erosion. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-01-01 2023-03-01T21:05:03Z 2023-03-01T21:05:03Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/1678-7757-2021-0698 Journal of applied oral science : revista FOB, v. 30, p. e20210698-. 1678-7765 http://hdl.handle.net/11449/241477 10.1590/1678-7757-2021-0698 2-s2.0-85135501628 |
url |
http://dx.doi.org/10.1590/1678-7757-2021-0698 http://hdl.handle.net/11449/241477 |
identifier_str_mv |
Journal of applied oral science : revista FOB, v. 30, p. e20210698-. 1678-7765 10.1590/1678-7757-2021-0698 2-s2.0-85135501628 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of applied oral science : revista FOB |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
e20210698 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799964657971101696 |