In vitro genotoxicity assessment of caffeic, cinnamic and ferulic acids

• Autor(a) principal: Maistro, Edson Luis [UNESP]
• Data de Publicação: 2011
• Outros Autores: Angeli, J. P. F., Andrade, S. F., Mantovani, M. S.
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://dx.doi.org/10.4238/vol10-2gmr1278; http://hdl.handle.net/11449/10816
• topic: Caffeic acid; Cinnamic acid; Ferulic acid; Comet assay; Micronucleus test; Clastogenic effects

Phenols are a large and diverse class of compounds, many of which occur naturally in a variety of food plants; they exhibit a wide range of biological effects, including antibacterial, anti-inflammatory, antiallergic, hepatoprotective, antithrombotic, antiviral, anticarcinogenic, and vasodilatory actions. We examined the genotoxic and clastogenic potential of three phenolic compounds: caffeic, cinnamic and ferulic acids, using the comet and micronucleus assays in vitro. Drug-metabolizing rat hepatoma tissue cells (HTCs) were used. Three different concentrations (50, 500 and 1500 mu M) of these phenolic acids were tested on the HTCs for 24 h. The caffeic, cinnamic and ferulic acids were not genotoxic by the comet assay (P > 0.05). However, the micronucleus test showed an increase in the frequency of micronucleated cells for the three compounds, indicating that these substances have clastogenic effects in HTC.